scholarly journals Genetic Diversity in the Camel Tick <i>Hyalomma dromedarii</i> (Acari: Ixodidae) Based on Mitochondrial Cytochrome c Oxidase Subunit I (COI) and Randomly Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR)

2018 ◽  
Vol 06 (04) ◽  
pp. 285-294
Author(s):  
Mohammad Ali Al-Deeb ◽  
Mohamed Rizk Enan
Keyword(s):  
Genetic Diversity ◽  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Mitochondrial Cytochrome ◽  
Randomly Amplified Polymorphic Dna ◽  
Hyalomma Dromedarii ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Camel Tick ◽  
Polymerase Chain

Genetic diversity among microsporidian isolates from the silkworm, Bombyx mori, as revealed by randomly amplified polymorphic DNA (RAPD) markers

2011 ◽  
Vol 56 (4) ◽  
Author(s):  
B. Surendra Nath ◽  
W. Hassan ◽  
S. Nageswara Rao ◽  
N. Vijaya Prakash ◽  
S. Gupta ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Type Species ◽  
Rapd Markers ◽  
Randomly Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Random Amplification ◽  
Major Cluster ◽  
Sources Of Infection ◽  
Polymerase Chain

AbstractRandom amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) was carried out to assess the genetic diversity of five new microsporidian isolates viz., NIWB-11bp, NIWB-12n, NIWB-13md, NIWB-14b and NIWB-15mb identified from the silkworms. A type species, NIK-1s_mys was used as control for comparison. Differences in the spore shape, length and width were observed. Of the 30 decamer random primers tested, 22 primers gave repeatable RAPD profiles and yielded a total of 143 fragments, of which 78 were polymorphic (55%). The resulting data was used to derive genetic similarity values for constructing a dendrogram. The neighbour joining method based on Dice coefficients indicate a major cluster comprising NIK-1s_mys, NIWB-11bp and NIWB-12n, whereas NIWB-13md, NIWB-14b and NIWB-15mb appear to be different from each other as well from the major cluster mentioned above which includes the type species (NIK-1s_mys). Based on the reproducibility of RAPD profiles, we are able to identify these microsporidians as different isolates. The RAPD technique may be useful in detecting sources of infection of this economically important domestic insect.

DNA FINGERPRINTING OF SINGLE APHID EMBRYOS BY RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION (RAPD–PCR)

1999 ◽  
Vol 131 (2) ◽  
pp. 229-230 ◽  
Author(s):  
C.K. Chan ◽  
D.J. Petersen ◽  
T.C. Vrain
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Acyrthosiphon Pisum ◽  
Random Amplified Polymorphic Dna ◽  
Aphis Gossypii ◽  
Aphis Fabae ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Laboratory Colonies ◽  
Polymerase Chain

Extraction of DNA from whole aphids, in combination with random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) (Williams et al. 1990) markers can detect interspecific and intraspecific genetic variation (Black et al. 1992; Cenis et al. 1993). However, these techniques entail destructive sampling of fresh or preserved specimens. To allow experimental replication from a single sample while preserving the same aphid for morphometrical or karyotyping analyses, we describe a technique for RAPD-PCR using DNA from single aphid embryos. We evaluated the usefulness and reliability of single-embryo analysis, using four species of our laboratory colonies, namely Acyrthosiphon pisum (Harris), Aphis fabae Scopoli, Aphis frangulae group, and Aphis gossypii Glover.

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