scholarly journals Detection of Verocytotoxin-Producing Escherichia coli O157 by Polymerase Chain Reaction Assay After Immunomagnetic Separation-Plating.

2001 ◽  
Vol 6 (1) ◽  
pp. 43-47 ◽  
Author(s):  
SHIGEKO UEDA ◽  
YOSHIHIRO KUWABARA
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Assay ◽  
Immunomagnetic Separation ◽  
Escherichia Coli O157 ◽  
Chain Reaction ◽  
Polymerase Chain

Rapid and sensitive immunomagnetic separation–polymerase chain reaction method for the detection of Escherichia coli O157[ratio ]H7 in raw milk and ice-cream

1997 ◽  
Vol 64 (1) ◽  
pp. 87-93 ◽  
Author(s):  
CHRISTOPHER M. GOODING ◽  
PRABHAKARA V. CHOUDARY
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Ice Cream ◽  
Raw Milk ◽  
Immunomagnetic Separation ◽  
Polymerase Chain Reaction Method ◽  
Escherichia Coli O157 ◽  
Agarose Gel Electrophoresis ◽  
Chain Reaction ◽  
Polymerase Chain

Escherichia coli O157[ratio ]H7 in spiked samples of raw milk and ice-cream was enriched in tryptic soy broth for 4 h, captured by immunomagnetic separation, subjected to amplification by polymerase chain reaction of parts of the verotoxin genes (SLT-I and SLT-II), and detected by agarose gel electrophoresis. Using this method, as few as 1 cfu Esch. coli O157[ratio ]H7/g food could be detected in <10 h.

A rapid (one day), sensitive real-time polymerase chain reaction assay for detecting Escherichia coli O157:H7 in ground beef

2006 ◽  
Vol 52 (10) ◽  
pp. 992-998 ◽  
Author(s):  
Jane Holicka ◽  
Rebecca A Guy ◽  
Anita Kapoor ◽  
David Shepherd ◽  
Paul A Horgen
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Polymerase Chain Reaction Assay ◽  
Ground Beef ◽  
Automatic Process ◽  
Escherichia Coli O157 ◽  
Chain Reaction ◽  
E Coli ◽  
Polymerase Chain

The purpose of this study was to apply our rapid, integrated double enrichment 5′ nuclease real-time polymerase chain reaction assay for the detection of Escherichia coli O157:H7 and evaluate its efficacy. The assay targeted ground beef, an important vehicle in disease epidemiology. The assay reliably determined in 8 h the presence of E. coli O157:H7 in ground beef at the level of 1 colony-forming unit (CFU)/g. The sensitivity and specificity of the assay were compared with that of standard enrichment diagnostic techniques. A correlation of 100% in detection was achieved to the limit of 1 CFU/g. This assay can be used as a rapid, automatic process for identification of E. coli O157:H7 in ground beef or can be integrated with standard culture procedures, resulting in considerable cost and time savings.Key words: real-time PCR, E. coli O157:H7, ground beef, molecular diagnostics, rapid O157:H7 assay.

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