Regulatory effect of Tripterygium wilfordii polycoride (TWP) towards TLR4/ MyD88 independent pathway in TNBS/ethanol ulcerative colitis (UC) rat model

2016 ◽  
Keyword(s):  
Ulcerative Colitis ◽  
Rat Model ◽  
Regulatory Effect ◽  
Tripterygium Wilfordii

Carvedilol Attenuates Inflammatory Biomarkers and Oxidative Stress in a Rat Model of Ulcerative Colitis

2015 ◽  
Vol 76 (4) ◽  
pp. 204-214 ◽  
Author(s):  
Amal J. Fatani ◽  
Khaled A. Al-Hosaini ◽  
Mohammed M. Ahmed ◽  
Hatem M. Abuohashish ◽  
Mihir Y. Parmar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ulcerative Colitis ◽  
Rat Model ◽  
Inflammatory Biomarkers ◽  
And Oxidative Stress

scholarly journals Lung and Intestine: A Specific Link in an Ulcerative Colitis Rat Model

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Yuan Liu ◽  
Xin-Yue Wang ◽  
Xue Yang ◽  
Shan Jing ◽  
Li Zhu ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Lung Tissue ◽  
Rat Model ◽  
Close Association ◽  
Trinitrobenzene Sulfonic Acid ◽  
Microvascular Endothelium ◽  
Functional Changes ◽  
Liver And Kidney ◽  
Embryonic Origin ◽  
Endothelial Growth Factor

Background. To investigate the link and mechanisms between intestine and lung in the ulcerative colitis (UC) rat model.Materials and Methods. We used the UC rat model by immunological sensitization combined with local 2, 4, 6-trinitrobenzene sulfonic acid (TNBS) in 50% ethanol enema, observed dynamically animal general state and body weight, examined the histological and functional changes in the colon, lung, liver, and kidney tissues, and detected microvascular endothelium response towards inflammation characterized with the expression of iNOS, TXB2, P-selectin, ICAM-1, and vascular endothelial growth factor A (VEGF-A) in the colon and lung tissue.Results. Pulmonary function results suggested ventilator disorder, and pathological findings showed interstitial pneumonia. There were no significant changes in the liver and kidney function and histopathology. The colon and lung tissue iNOS, TXB2, P-selectin, ICAM-1, and VEGF-A expression of the model rats was significantly higher than the normal rats at both time points.Conclusions. Our study is the first to demonstrate the close association between the large intestine and lung in the immune-TNBS-ethanol-induced UC rat model. Different organs and tissues with the same embryonic origin may share the same pathological specificities in a disease. The present study provided a new way of thinking for pathological changes in clinical complex diseases manifested with multiorgan damage.

scholarly journals Moxibustion Inhibits the Expression of Colonic NLRP3 through miR7/RNF183/NF-κB Signaling Pathway in UC Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Xi-Ying Li ◽  
Yan-Ting Yang ◽  
Yue Zhao ◽  
Xie-He Kong ◽  
Guang Yang ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Disease Activity ◽  
Signaling Pathway ◽  
Rat Model ◽  
Activity Index ◽  
Disease Activity Index ◽  
Moxa Stick Moxibustion ◽  
Control Group ◽  
Colon Injury ◽  
Moxa Stick

Background. Moxibustion has been recognized as an effective approach for ulcerative colitis, yet its mechanism is not clear. The research aimed to investigate the influence of moxibustion on the activation of NLRP3 inflammasome and its mechanism in treating ulcerative colitis by observing miR7/RNF183 inducing IκB α ubiquitination to regulate NF-κB signaling pathway in an ulcerative colitis rat model. Methods. An ulcerative colitis rat model was established by unlimited access to self-administration of 3.5% (w/v) dextran sulfate sodium solution. Mild moxibustion was applied to bilateral Tianshu points (ST25) in the moxa-stick moxibustion group; rats in the control group were intervened by intraperitoneal injection of ubiquitination inhibitor, MG132. The disease activity index was determined at the end of the intervention; colon injury was observed and scored after hematoxylin-eosin staining; the immunohistochemical method was adopted to detect the expressions of colonic IL-1β and NLRP3 proteins; Western blot determined the expressions of RNF183, IκB α, and NF-κB p65 proteins in the colon; the immunofluorescence test was used to observe the coexpression of IκB α/ubiquitin and IκB α/RNF183 proteins in the colon; immunoprecipitation assay was adopted to observe the interaction between IκB α and RNF183 proteins; and quantitative real-time polymerase chain reaction determined the expression of colonic miR7. Results. Moxibustion lowered the disease activity index, manifesting as restored colonic tissue and reduced inflammatory reaction, and decreased expression levels of NLRP3 and IL-1β proteins, compared with the model group. It also reduced colonic expression of NF-κB p65 protein, together with the increased level of IκB α protein and weaker expression levels of ubiquitin and RNF183 proteins and mRNAs and stronger expression of miR7. There were no significant differences between the moxa-stick moxibustion group and the control group except the expressions of RNF183 protein and mRNA and miR7. Conclusion. Moxibustion encourages the recovery of colon injury probably by regulating the expression of NLRP3 protein in ulcerative colitis rats through miR7/RNF183/NF-κB signaling pathway.

Effects of N-acetylcysteine on acetic-acid-induced ulcerative colitis in experimental rat model

2017 ◽  
Vol 45 (1) ◽  
pp. 36
Author(s):  
AsmaaMahmoud Ghalwash ◽  
NemaAli Soliman ◽  
HalaElsaid Hamouda ◽  
SalwaAkhnoukh Absakhron
Keyword(s):  
Ulcerative Colitis ◽  
Acetic Acid ◽  
Rat Model ◽  
Experimental Rat Model

scholarly journals P167 CHARACTERIZATION OF THE NEUROCHEMICAL AND NEUROMUSCULAR ADAPTIONS IN A RAT MODEL OF ULCERATIVE COLITIS

2007 ◽  
Vol 1 (1) ◽  
pp. 46
Author(s):  
M. Auli ◽  
Y. Nasser ◽  
W. Ho ◽  
C.M. Keenan ◽  
J.F. Burgueño ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Rat Model
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