USE OF AN IRON SUPEROXIDE DISMUTASE EXCRETED BY TRYPANOSOMA CRUZI IN THE DIAGNOSIS OF CHAGAS DISEASE: SEROPREVALENCE IN RURAL ZONES OF THE STATE OF QUERETARO, MEXICO

2005 ◽  
Vol 73 (3) ◽  
pp. 510-516 ◽  
Author(s):  
MARÍA E. VILLAGRÁN ◽  
CLOTILDE MARÍN ◽  
ISABEL RODRÍGUEZ-GONZALEZ ◽  
MANUEL SÁNCHEZ-MORENO ◽  
JOSE A. DE DIEGO
Keyword(s):  
Superoxide Dismutase ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
The State ◽  
Iron Superoxide Dismutase

scholarly journals Triatomine Fauna and Recent Epidemiological Dynamics of Chagas Disease in an Endemic Area of Northeast Brazil

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Cláudia M. Melo ◽  
Ana Carla F. G. Cruz ◽  
Antônio Fernando V. A. Lima ◽  
Luan R. Silva ◽  
Rubens R. Madi ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Disease Control ◽  
Chagas Disease ◽  
Control Program ◽  
The State ◽  
Positive Serology ◽  
Serological Tests ◽  
Systemic Analysis ◽  
South Central ◽  
Disease Control Program

Updated information of the dispersion dynamics of Chagas disease (CD) and a systemic analysis of these data will aid the early identification of areas that are vulnerable to transmission and enable efficient intervention. This work synthesized spatiotemporal information regarding triatomine fauna and analyzed this information in combination with the results from serological tests to elucidate the epidemiological panorama of CD in the state of Sergipe, Brazil. This is a retrospective analytical study that utilized information from the database of the National Chagas Disease Control Program. Between 2010 and 2016, 838 triatomines of eight species, namely, Panstrongylus geniculatus, which was first recorded in the state of Sergipe, Panstrongylus lutzi, P. megistus, Triatoma brasiliensis, T. pseudomaculata, T. tibiamaculata, T. melanocephala, and Rhodnius neglectus, were collected. Optical microscopy revealed that 13.2% of triatomines examined were infected by Trypanosoma cruzi-like flagellates. The distribution of triatomines exhibits an expanding south-central to northern dispersion, with a preference for semiarid and agreste areas and occasional observations in humid coastal areas due to anthropogenic actions reflected in the environment. Of the human cases analyzed from 2012 to 2016, 8.3% (191/2316) presented positive serology for Trypanosoma cruzi, and this proportion showed a gradual increase in the southern center of the state and new notifications in coastal regions. There is a need for intensification and continuity of the measures adopted by the Chagas Disease Control Program in Sergipe, identifying new priority areas for intervention and preferential ecotopes of the vectors, considering the occurrence of positive triatomines intradomicilliary and a source of new triatomines in the peridomiciles.

Efficient Inhibition of Iron Superoxide Dismutase and of Trypanosoma cruzi Growth by Benzo[g]phthalazine Derivatives Functionalized with One or Two Imidazole Rings

2008 ◽  
Vol 51 (6) ◽  
pp. 1962-1966 ◽  
Author(s):  
Ana M. Sanz ◽  
Fernando Gómez-Contreras ◽  
Pilar Navarro ◽  
Manuel Sánchez-Moreno ◽  
Samira Boutaleb-Charki ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Trypanosoma Cruzi ◽  
Iron Superoxide Dismutase

scholarly journals An in vitro iron superoxide dismutase inhibitor decreases the parasitemia levels of Trypanosoma cruzi in BALB/c mouse model during acute phase

2015 ◽  
Vol 5 (3) ◽  
pp. 110-116 ◽  
Author(s):  
Francisco Olmo ◽  
Kristína Urbanová ◽  
Maria Jose Rosales ◽  
Ruben Martín-Escolano ◽  
Manuel Sánchez-Moreno ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Mouse Model ◽  
Trypanosoma Cruzi ◽  
Acute Phase ◽  
Iron Superoxide Dismutase

scholarly journals Mitochondrial superoxide radicals mediate programmed cell death in Trypanosoma cruzi: cytoprotective action of mitochondrial iron superoxide dismutase overexpression

2007 ◽  
Vol 403 (2) ◽  
pp. 323-334 ◽  
Author(s):  
Lucía Piacenza ◽  
Florencia Irigoín ◽  
María Noel Alvarez ◽  
Gonzalo Peluffo ◽  
Martin C. Taylor ◽  
...  
Keyword(s):  
Cell Death ◽  
Superoxide Dismutase ◽  
Programmed Cell Death ◽  
Trypanosoma Cruzi ◽  
Early Period ◽  
Pentose Phosphate ◽  
Iron Superoxide Dismutase ◽  
Mitochondrial Superoxide ◽  
Glucose Flux ◽  
Mitochondrial Aconitase

Trypanosoma cruzi undergo PCD (programmed cell death) under appropriate stimuli, the mechanisms of which remain to be established. In the present study, we show that stimulation of PCD in T. cruzi epimastigotes by FHS (fresh human serum) results in rapid (<1 h) externalization of phosphatidylserine and depletion of the low molecular mass thiols dihydrotrypanothione and glutathione. Concomitantly, enhanced generation of oxidants was established by EPR and immuno-spin trapping of radicals using DMPO (5,5-dimethylpyrroline-N-oxide) and augmentation of the glucose flux through the pentose phosphate pathway. In the early period (<20 min), changes in mitochondrial membrane potential and inhibition of respiration, probably due to the impairment of ADP/ATP exchange with the cytosol, were observed, conditions that favour the generation of O2•−. Accelerated rates of mitochondrial O2•− production were detected by the inactivation of the redox-sensitive mitochondrial aconitase and by oxidation of a mitochondrial-targeted probe (MitoSOX). Importantly, parasites overexpressing mitochondrial FeSOD (iron superoxide dismutase) were more resistant to the PCD stimulus, unambiguously indicating the participation of mitochondrial O2•− in the signalling process. In summary, FHS-induced PCD in T. cruzi involves mitochondrial dysfunction that causes enhanced O2•− formation, which leads to cellular oxidative stress conditions that trigger the initiation of PCD cascades; moreover, overexpression of mitochondrial FeSOD, which is also observed during metacyclogenesis, resulted in cytoprotective effects.

Synthetic single and double aza-scorpiand macrocycles acting as inhibitors of the antioxidant enzymes iron superoxide dismutase and trypanothione reductase in Trypanosoma cruzi with promising results in a murine model

RSC Advances ◽  
2014 ◽  
Vol 4 (110) ◽  
pp. 65108-65120 ◽  
Author(s):  
F. Olmo ◽  
M. P. Clares ◽  
C. Marín ◽  
J. González ◽  
M. Inclán ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Survival Rate ◽  
Trypanosoma Cruzi ◽  
Murine Model ◽  
Trypanothione Reductase ◽  
Iron Superoxide Dismutase

Synthetic scorpiand-like azamacrocycles selectively inhibit SOD and TR enzymes of Trypanosoma cruzi in mice causing death of the parasites and increasing the mouse survival rate after infection and treatment.

scholarly journals Detection of Trypansoma cruzi in Kissing Bugs (Hemiptera: Reduviidae: Triatominae) Collected Across Oklahoma

2022 ◽  
Author(s):  
Kelly E Allen ◽  
Megan W Lineberry
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Causative Agent ◽  
The State ◽  
Vertebrate Species ◽  
Human Dna ◽  
Mucous Membranes ◽  
Insect Collection

Abstract Trypanosoma cruzi is the causative agent of Chagas disease in humans and dogs in the Americas. Transmission predominantly occurs via the feces of infected kissing bugs (Hemiptera: family Reduviidae; subfamily Triatominae) contaminating bite site wounds or mucous membranes. To better understand Chagas disease entomologic risk in Oklahoma, kissing bugs collected from within the state were tested for T. cruzi DNA. Data including county of insect collection, species and instar, and specific locations where specimens were found were collated. Triatomines were also tested by PCR to potentially identify DNA of vertebrate species on which specimens had recently fed. In total, 110 kissing bugs from 22 counties were tested. All triatomines were identified as Triatoma sanguisuga nymphs or adults, with the exception of one possible T. lecticularia adult. Trypanosoma cruzi DNA was detected in 22 (20%) triatomines from 12 counties spanning the state. The majority of T. cruzi PCR positive kissing bugs were found inside homes or associated structures (i.e., garages, porches). Vertebrate DNA was identified in 27 (24.5%) triatomines, with human DNA detected in 25 (92.6%) of these specimens, and canine and raccoon DNA detected in one specimen each (3.7%). Two specimens tested positive for both T. cruzi and human DNA and one specimen tested positive for both T. cruzi and raccoon DNA. Results from this study indicate that kissing bugs carrying T. cruzi are widespread in Oklahoma, that positive kissing bugs infest homes and associated structures, and that human-vector, canine-vector, and wildlife-vector contact all occur within the state.

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