Effects of Beauveria bassiana on Survival, Blood-Feeding Success, and Fecundity of Aedes aegypti in Laboratory and Semi-Field Conditions

This research is about effectivity analysis of two kinds of fungi i.e. Beauveria bassiana and Metharrizium anisopliae as Aedes aegyptilarvacide, it has been conducted at laboratories Zoology in Biology Department, Mathematics and Natural Sciences Faculty, Syiah KualaUniversity. Aims of this research were estimating maximum time storage of fungi powder as effective larvacide and the number of bacteriacolonies were found in water used for experiment. This research was used Completely Randomized Design (CRD). The experiments consistof two factors i.e kind and saving time of fungi. The result show that the longer the storage time of Beauveria bassiana the more ineffectiveit was as larvacide and the least fungi colonies were found. Metharrizium anisopliae on the other hand, the longer it was kept, the moreeffective it was as larvacide and the more fungi colonies were found.

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AKTIVITAS LARVISIDAL EKSTRAK ETIL ASETAT DAN HEKSANA DARI FILTRAT Beauveria bassiana TERHADAP Aedes aegypti

BERITA BIOLOGI ◽

10.14203/beritabiologi.v18i3.3618 ◽

2019 ◽

Vol 18 (3) ◽

Author(s):

I Nyoman Pugeg Aryantha ◽

Wahyu Setyaji Dwiantara

Keyword(s):

Aedes Aegypti ◽

Ethyl Acetate ◽

Beauveria Bassiana ◽

Larvicidal Activity ◽

Control Agent ◽

Probit Analysis ◽

Hexane Extract ◽

Potato Dextrose Broth ◽

Synthetic Pesticides ◽

Biological Insect Control

Beauveria bassiana produces several metabolites that are toxic to insects so that it can be used as a biological insect control agent as an alternative to synthetic pesticides. The aim of this study was to determine the larvicidal activity of ethyl acetate and hexane extract from B. bassiana filtrate culture against Aedes aegypti 2nd instar larvae. This research was it cooked by determining the optimum age of spore inoculum of B. bassiana on the Potato Dextrose Agar (PDA) based on the number of spores and its viability. Afterwards, we determine the incubation time of B. bassiana in the Potato Dextrose Broth (PDB) in order to obtain filtrate culture which have highest mortality effects against Ae. aegypti 2nd instar larvae. B. bassiana filtrate culture was extracted with hexane and ethyl acetate and tested aegypti for larvicidal activity with a concentration range of 50, 100, 200, 300 ppm. The LC50 value was carried out by probit analysis. The results showed that ninth day old culture in the PDA was the optimum age of spore inoculum with the spore number and viability were 2.54 x 107 spore/mL and 93.46% respectively. The filtrate of sixth day old culture in PDB medium gave 100% mortality against 2nd instar Ae. Aegypti larvae. LC50 values of ethyl acetate and hexane extract were 117.28 dan 287.09 ppm. These results showed that the ethyl acetate and hexane extract of B. bassiana filtrate culture have biopesticide potential against 2nd instar Ae. aegypti larvae.

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The Use of Metarhizium anisopliae and Beauveria bassiana as Fungal Pathogen to Control the Growth of Aedes aegypti Population: A Preliminary Result

3rd International Conference on Chemical, Agricultural and Medical Sciences (CAMS-2015) Dec. 10-11, 2015 Singapore ◽

10.15242/iicbe.c1215008 ◽

2015 ◽

Keyword(s):

Aedes Aegypti ◽

Beauveria Bassiana ◽

Metarhizium Anisopliae ◽

Fungal Pathogen ◽

Aegypti Population

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Characterization of a vasodilator from the salivary glands of the yellow fever mosquito Aedes aegypti

Journal of Experimental Biology ◽

10.1242/jeb.165.1.61 ◽

1992 ◽

Vol 165 (1) ◽

pp. 61-71 ◽

Cited By ~ 4

Author(s):

J. M. Ribeiro

Keyword(s):

Salivary Gland ◽

Aedes Aegypti ◽

Salivary Glands ◽

Posterior Part ◽

Aortic Ring ◽

Blood Feeding ◽

Reversed Phase ◽

Relative Molecular Mass ◽

Vasodilatory Activity ◽

Carboxy Terminal

Salivary gland homogenates and oil-induced saliva of the mosquito Aedes aegypti dilate the rabbit aortic ring and contract the guinea pig ileum. The vasodilatory activity is endothelium-dependent, heat-stable, sensitive to both trypsin and chymotrypsin treatments, and both smooth muscle activities cross-desensitize to the tachykinin peptide substance P. Both bioactivities co-elute when salivary gland homogenates are fractionated by reversed-phase HPLC. Molecular sieving chromatography indicates a relative molecular mass of 1400. A monoclonal antibody specific to the carboxy terminal region of tachykinins reacts with material in the posterior part of the central lobe of paraformaldehyde-fixed salivary glands. The presence of a vasodilatory peptide of the tachykinin family in the salivary glands of A. aegypti is proposed and its role in blood feeding is discussed.

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