scholarly journals Validation of an UV-Visible spectrophotometry assay method for the determination of chlorpheniramine maleate tablets without prior extraction

2021 ◽  
Vol 15 (1) ◽  
pp. 273-281
Author(s):  
Linda Carole Djiambeu Chawe ◽  
Nassifatou Koko Tittikpina ◽  
Serigne Momar Ndiaye ◽  
Amadou Diop ◽  
Bara Ndiaye ◽  
...  
Keyword(s):  
Raw Material ◽  
Assay Method ◽  
Tolerance Limits ◽  
Chlorpheniramine Maleate ◽  
Visible Spectrophotometry ◽  
Accuracy Profile ◽  
Upper Limits ◽  
Uv Visible ◽  
Lower Limits

Methods proposed by pharmacopeias to check the quality of chlorpheniramine maleate tablets are multi steps methods which involve extraction and present issues with repeatability. An alternative method is proposed with a sensitive, reliable, simple and rapid UV-VISIBLE spectrophotometry method developed for the determination without extraction of chlorpheniramine maleate in tablets. The method was validated using the accuracy profile approach with an accuracy ranging from 99.70 to 100.46%. Analysis was done using 0.25 mol/L sulfuric acid, distilled water, and raw material in a room at 23 °C for 25 to 30 minutes. Chlorpheniramine maleate concentration varied from 0.018 to 0.03 mg/mL. The method was found to be specific with the appearance of the corresponding maxima at 265 nm and a correlation coefficient (R2 ) of 0.9993. Limits of detection and quantification were respectively 1.39 × 10-4and 2.26 × 10-3 mg/mL. The tolerance limits for the different concentration levels (75, 100 and 125%) were respectively: 88.37; 92.74 and91.62% for lower limits and 111.03; 108.17 and 108.02% for upper limits. It was observed that the tolerance limits were within the limits of acceptability set at 20%. Consequently, the method was declared valid and reliable for routine analysis of chlorpheniramine maleate in tablets containing chlorphenamine as active principle.Keywords : Chlorpheniramine maleate, tablets, accuracy profile, assay method without extraction, validation

scholarly journals Development and validation of a UV-Visible method for the determination of the active principle Efavirenz in tablets

2020 ◽  
Vol 14 (1) ◽  
pp. 279-288
Author(s):  
Nassifatou Koko Tittikpina ◽  
Thierno Mouhamed Wane ◽  
Diery Diouf ◽  
Khadidiatou Thiam ◽  
Amadou Diop ◽  
...  
Keyword(s):  
Active Principle ◽  
Tolerance Limits ◽  
African Countries ◽  
Accuracy Profile ◽  
Antiretroviral Drug ◽  
Upper Limits ◽  
Uv Visible ◽  
Development And Validation ◽  
Lower Limits

Methods proposed by pharmacopeias to control the quality of Efavirenz, an antiretroviral drug used in the treatment of AIDS are based on expensive equipment which are most of the time not available in African countries. To solve this issue, using UV-visible spectrophotometry, a cheap and easy-to-use device available in many laboratories on the African continent; a sensitive, reliable, simple and rapid method has been validated for the determination of the active substance Evafirenz in Efavirenz tablets, to control their quality by checking its dosage. The accuracy profile approach was adopted. The response function gave a correlation coefficient R2 = 0.9987. The detection and quantification limits were 0.15625 μg / mL and 0.515625 μg / mL, respectively. The acceptability limit has been set at 15%. The tolerance limits for the different concentration levels (80, 100 and 120%) are represented respectively as follows: lower limits 86.2; 90.3 and 96.1%; upper limits 100.2; 106.3 and 113.7%. The tolerance limits are within the acceptability limits for values around 100% concentration (7 μg / mL). Therefore, the method was declared valid and reliable for the analysis of Efavirenz in Efavirenz tablets and could routinely be used in laboratories for the quality control of EFAVIRENZ drug. © 2020 International Formulae Group. All rights reserved. Keywords: Efavirenz, accuracy profile, spectrophotometry, pharmaceutical formulation

Design of a novel optical sensor for determination of trace amounts of copper by UV–visible spectrophotometry in real samples

2017 ◽  
Vol 32 (3) ◽  
pp. e4110
Author(s):  
Eslam Pourbasheer ◽  
Somayeh Morsali ◽  
Zhila Azari ◽  
Mohammad Ali Karimi ◽  
Mohammad Reza Ganjali
Keyword(s):  
Optical Sensor ◽  
Real Samples ◽  
Visible Spectrophotometry ◽  
Uv Visible

Determination of Functional Components of Peanut Skins Pigment

2013 ◽  
Vol 807-809 ◽  
pp. 2035-2042
Author(s):  
Lei Du ◽  
Xin Hua Li ◽  
Ying Liu
Keyword(s):  
Ethyl Acetate ◽  
Chemical Reaction ◽  
Ethanol Solution ◽  
Macroporous Resin ◽  
Visible Spectrophotometry ◽  
Preliminary Identification ◽  
Uv Visible ◽  
Peanut Skins ◽  
Functional Components

The pigments in black and red peanut skins were comparative analyzed in this paper. The pigments were extracted with ethanol solution, purified by AB-8 macroporous resin, and black peanut skins pigment (BPSP) was separated by ethyl acetate to obtain different compositions. The compositions of pigments were qualitatively analyzed by special chemical reaction of organics and UV-visible spectrophotometry. The results show that the compositions of BPSP are mainly anthocyanins and proanthocyanidins, and the contents are 9.39% and 16.62%, respectively. The proanthocyanidins are found in red peanut skins pigment (RPSP), and the content is 24.51%, and no anthocyanin is checked out. It is convenient, rapid, economical and effective for special chemical reaction of organics and UV-visible spectrophotometry, and it is suitable for preliminary identification of natural organics structure.

UV-Visible Spectrophotometry with Arsenazo III for the Determination of Samarium

2019 ◽  
Vol 86 (3) ◽  
pp. 542-548
Author(s):  
Xing-Min Guo ◽  
Qi-Cao Yan ◽  
Xiao-Ting Meng ◽  
Rui-Xin Ma
Keyword(s):  
Arsenazo Iii ◽  
Visible Spectrophotometry ◽  
Uv Visible

scholarly journals Determination of Triiodide Ion Concentration Using UV-Visible Spectrophotometry

2014 ◽  
Vol 26 (13) ◽  
pp. 4084-4086 ◽  
Author(s):  
Sang-Hyuk Jung ◽  
Jei-Won Yeon ◽  
Yong Kang ◽  
Kyuseok Song
Keyword(s):  
Ion Concentration ◽  
Visible Spectrophotometry ◽  
Uv Visible

Liquid Chromatographic Determination of Cromolyn Sodium and Related Compounds in Raw Materials

1994 ◽  
Vol 77 (6) ◽  
pp. 1439-1442 ◽  
Author(s):  
Randy M Duhaime ◽  
Lorna K Rollins ◽  
Dennis J K Gorecki ◽  
Edward G Lovering
Keyword(s):  
Mobile Phase ◽  
Raw Materials ◽  
Chromatographic Determination ◽  
Cromolyn Sodium ◽  
Raw Material ◽  
Assay Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Related Compounds

Abstract Liquid chromatographic methods for the assay of cromolyn sodium and 3 related compounds were developed. The methods, which differ only in the composition of the mobile phase, use an octadecylsilane bonded-phase column and an acetonitrile–water–cetyltrimethylammonium bromide mobile phase. Minimum quantitatable amounts of the related compounds were less than 0.05%; assay method precision was better than 1 %. Impurity levels in raw material samples from 4 manufacturers were less than 0.1 %.

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