scholarly journals Monitoring the in-situ Bioremediation of Spend engine-oil contaminated soil After Irrigation with Fermented Chicken-droppings

2020 ◽  
Vol 24 (3) ◽  
pp. 411-416
Author(s):  
V.E. Okpashi ◽  
O.A. Ushie ◽  
F.E. Abeng ◽  
I.H. Inyang
Keyword(s):  
Soil Fertility ◽  
Contaminated Soil ◽  
Low Cost ◽  
Positive Control ◽  
Engine Oil ◽  
Control Group ◽  
Used Engine Oil ◽  
Uncontaminated Soil ◽  
Tandem Mass Spectroscopy

Preparation for the bioremediation of petroleum contaminants is explored to provide a low-cost and capable strategy for biodegradation of  contaminants and renewal of soil fertility. In this study, fermented chicken droppings were used as novel in-situ bio-stimulants and bio- augmentation materials. The investigation determines the capability of fermented chicken droppings to biodegrade the residual total petroleum  hydrocarbon compounds in-used engine oil contaminated soil – in the case of auto mechanic shops. The soil was collected at 10 cm depth from the ground, air-dried and sieved with 2.5mm mesh. A 4 kg of soil was weighed into 13 perforated buckets to allow aeration and prevent waterlogging. The setup consists of three replicates that were spiked with 150 ml of used engine oil. 500 ml of the fermented chicken droppings were used to irrigate the contaminated soil at ratio 1:8 periodically for every 3 days per irrigation for 21 days. The uncontaminated soil used as the control group was irrigated with normal water. The TPH composition of the contaminated and remediated soil samples was screened using gas Chromatography tandem mass spectroscopy. Results show that the uncontaminated soil (A-group) had C10 - 24.058 ± 0.02 ppm, C12 - 37.327 ± 0.01 ppm, C14 - 28.515 ± 0.02 ppm and C16 - 12.097 ± 0.02 ppm, respectively out of about 35 TPH compounds that ought to be detected from C8 to C40. The Concentration of TPH in Contaminated soil before irrigation with Chicken droppings – positive control (ppm) B-group gave a significant qualitative and quantitative presence of TPHs in contaminated soil at varying concentrations. 36 TPHs were detected out of forty, starting from C10 - 1.836 ± 0.01 ppm to C38 -  50.150±0.01 ppm. Whereas, the Concentration of residual TPH in Contaminated soil after irrigation with Chicken droppings gave varying levels of residual TPHs ranging from C8 - 1.519 ± 0.02 ppm to C38 - 41.487 ±0.02 ppm. This also gave a resultant/differences in the degradation level of TPHs. Differences in TPH Concentration between before the irrigation and after irrigation of contaminated soil were calculated, C8 had - 0.317 ppm while another TPHs concentration varies accordingly. From the results, one can be deduced that despite other environmental factors that influence the degradation of TPHs, fermented chicken droppings showed great capability in the degradation of TPHs in the contaminated soil. Keywords: Bioremediation, auto-mechanic-yard, Soil-fertility, Used-engine-oil, fermented-chicken-droppings

scholarly journals Effect Of Agitated Chicken-Droppings for in Situ Bioremediation of Pahs in Spend Engine-Oil Contaminated Soil

2021 ◽  
pp. 1-9
Author(s):  
VE Okpashi ◽  
NE Etim ◽  
Inyang IH ◽  
UN Obeten
Keyword(s):  
Contaminated Soil ◽  
Oil Pollution ◽  
Sandy Loam ◽  
Low Cost ◽  
Ground Level ◽  
Positive Control ◽  
Negative Control ◽  
Engine Oil ◽  
Used Engine Oil ◽  
Normal Water

The populace are concern about how their environment is impacted by spend or used engine oil. The spills from spend engine oil posed serious problems to the land and aquatic habitats. Efficient methods but low-cost technique for remediating spends engine oil contaminated soil and restorations of soil fertility are being explored. In response to used engine oil pollution to the soil, the present investigation examined the comparative biodegradation level of spend engine oil contaminated soil using fermented chicken droppings. The soil sample was collected from the uncontaminated site 10 cm below ground level. The sandy/loam soil was sieved with 2.5 mm mesh. A 4 kg of the soil was weighed into each bucket and contaminated with spend engine oil, the soil was contaminated with 150 ml of the spend engine oil at a ratio of 1:27. A 27 kg of chicken droppings was mixed with 27 liters of water in a ratio of 1 kg: 1L. The mixture was left for 7days to ferment and increase the microbial load. The contaminated soil was irrigated using an experimental design that included uncontaminated soil that was irrigated using normal water (positive control), contaminated soil that was irrigated using normal water (negative control) and contaminated soil irrigated using fermented chicken droppings (test). Five hundred mL of the chicken droppings mixture was used to irrigate the soil for 21 days at an interval of 3 days per irrigation. Thereafter, the irrigated soil was screened with gas chromatography linked with mass spectroscopy. There was an observed change in contaminant concentration without fermented chicken droppings. The percentage of differences in the concentrations was recorded in × 10–5% with the presence of Naphthalene and Acenaphthylene with the percent difference of 2.0 × 10–5% and 5.0 × 10– 5%. Result revealed an appreciable decrease in PAHs level compared to the positive and negative control. The findings suggest the positive effect and rapid uptake of the contaminant in the application of fermented chicken droppings. J. Bio-Sci. 29(1): 01-09, 2021 (June)

Relative Bioremediation of Used engine oil Contaminated soil from an Industrialised area by Various microbes

2019 ◽  
Vol 12 (1) ◽  
pp. 331
Author(s):  
J. Sivakumar ◽  
C. Shanmuga Sundaram ◽  
L. Krishnasamy ◽  
U. S. Mahadeva Rao
Keyword(s):  
Contaminated Soil ◽  
Engine Oil ◽  
Used Engine Oil

scholarly journals Effective phenanthrene and pyrene biodegradation using Enterobacter sp. MM087 (KT933254) isolated from used engine oil contaminated soil

2018 ◽  
Vol 27 (3) ◽  
pp. 349-359 ◽  
Author(s):  
Zubairu Darma Umar ◽  
Abd Aziz Nor Azwady ◽  
Syaizwan Zahmir Zulkifli ◽  
Mustafa Muskhazli
Keyword(s):  
Contaminated Soil ◽  
Engine Oil ◽  
Used Engine Oil

scholarly journals Bioremediation of engine-oil contaminated soil using local residual organic matter

2019 ◽  
Author(s):  
Kawina Robichaud ◽  
Miriam Lebeau ◽  
Sylvain Martineau ◽  
Marc Amyot
Keyword(s):  
Contaminated Soil ◽  
Treatment Time ◽  
Low Cost ◽  
Carbon Sources ◽  
Engine Oil ◽  
Fertilizer Treatment ◽  
Biolog Ecoplates ◽  
Catabolic Diversity ◽  
Remediation Efficiency ◽  
Spent Grain

Soil remediation industries continue to seek technologies to speed-up treatment and reduce operating costs. Some processes are energy intensive and, in some cases, transport can be the main source of carbon emissions. Residual fertilizing matter (RFM), such as organic residues, have the potential to be beneficial bioremediation agents. Following a circular economy framework, we investigated the feasibility of sourcing RFMs locally to reduce transport and assess possible bioremediation efficiency gains. RFMs were recruited within 100 km of the treatment site: ramial chipped wood (RCW), horse manure (MANR) and brewer spent grain (BSG). They were added to the land treatment unit’s baseline fertilizer treatment (FERT, ‘F’) to measure if they improved the remediation efficiency of an engine oil-contaminated soil (7500 ± 100 mg kg-1). Results indicate that MANR-F was the only amendment more effective than FERT for PHC reduction, while emitting the least CO2overall. RCW-F was equivalent to FERT but retained more moisture. Although BSG contributed the most nitrogen to the soil, BSG-F retained excessive moisture, emitted more VOCs, contained less soil O2, and was less effective than the baseline treatment. Significantly more of the C16-C22fraction was removed (63 ± 22%) than all other fractions (C22-C28, C28-C34, C34-C40), which were equally removed. Microbial community-level physiological profiling (CLPP) was conducted with Biolog EcoplatesTM, and catabolic diversity differed between treatments (utilization rates of 31 carbon sources). MANR-F has the potential to increase PHC-remediation speed and efficiency compared to inorganic fertilizer alone. Other RFM promote moisture retention and diverse microbial catabolic activity. A variety of RFM are present across the globe and some can offer low-cost amendments to boost remediation efficiency, while reducing treatment time compared to traditional fertilizer-only methods.

In situ remediation of arsenic-rich mine tailings using slag zero valence iron

2020 ◽  
Vol 84 (3) ◽  
pp. 420-434
Author(s):  
Tingting Yue ◽  
Shu Chen ◽  
Jing Liu
Keyword(s):  
Photoelectron Spectroscopy ◽  
Solid Phase ◽  
Low Cost ◽  
Selective Extraction ◽  
Hunan Province ◽  
Control Group ◽  
X Ray Diffraction ◽  
Land Occupation ◽  
X Ray

AbstractArsenopyrite (FeAsS) and realgar (As4S4) are two common arsenic minerals that often cause serious environmental issues. Centralised treatment of arsenic-containing tailings can reduce land occupation and save management costs. The current work examined the remediation schemes of tailings from Hunan Province, China, where by different tailings containing arsenopyrite and realgar were blended with exogenous slag zero valence iron (ZVI). Introducing Fe-oxidising bacteria (Acidithiobacillus ferrooxidans) recreates a biologically oxidative environment. All bioleaching experiments were done over three stages, each for 7 days and the solid phase of all tests was characterised by scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy and selective extraction analyses. The results showed that the mixture group reduced arsenic release by 72.9–74.7% compared with the control group. The addition of 0.2 g ZVI clearly decreased arsenic release, and the addition of 4.0 g ZVI led to the lowest arsenic release among all tests. The decrease of arsenic released from the tailings was due to the adsorption and uptake of arsenic by secondary iron-containing minerals and Fe–As(V) secondary mineralisation. The addition of large amounts of ZVI reduced the arsenic detected in the amorphous Fe precipitates. Therefore, a low cost and integrated strategy to reduce arsenic release from tailings is to mix two typical tailings and apply exogenous slag ZVI, which can apply to the in situ remediation of two kinds or more arsenic-containing tailings.

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