scholarly journals Bioremediation of engine-oil contaminated soil using local residual organic matter

2019 ◽  
Author(s):  
Kawina Robichaud ◽  
Miriam Lebeau ◽  
Sylvain Martineau ◽  
Marc Amyot
Keyword(s):  
Contaminated Soil ◽  
Treatment Time ◽  
Low Cost ◽  
Carbon Sources ◽  
Engine Oil ◽  
Fertilizer Treatment ◽  
Biolog Ecoplates ◽  
Catabolic Diversity ◽  
Remediation Efficiency ◽  
Spent Grain

Soil remediation industries continue to seek technologies to speed-up treatment and reduce operating costs. Some processes are energy intensive and, in some cases, transport can be the main source of carbon emissions. Residual fertilizing matter (RFM), such as organic residues, have the potential to be beneficial bioremediation agents. Following a circular economy framework, we investigated the feasibility of sourcing RFMs locally to reduce transport and assess possible bioremediation efficiency gains. RFMs were recruited within 100 km of the treatment site: ramial chipped wood (RCW), horse manure (MANR) and brewer spent grain (BSG). They were added to the land treatment unit’s baseline fertilizer treatment (FERT, ‘F’) to measure if they improved the remediation efficiency of an engine oil-contaminated soil (7500 ± 100 mg kg-1). Results indicate that MANR-F was the only amendment more effective than FERT for PHC reduction, while emitting the least CO2overall. RCW-F was equivalent to FERT but retained more moisture. Although BSG contributed the most nitrogen to the soil, BSG-F retained excessive moisture, emitted more VOCs, contained less soil O2, and was less effective than the baseline treatment. Significantly more of the C16-C22fraction was removed (63 ± 22%) than all other fractions (C22-C28, C28-C34, C34-C40), which were equally removed. Microbial community-level physiological profiling (CLPP) was conducted with Biolog EcoplatesTM, and catabolic diversity differed between treatments (utilization rates of 31 carbon sources). MANR-F has the potential to increase PHC-remediation speed and efficiency compared to inorganic fertilizer alone. Other RFM promote moisture retention and diverse microbial catabolic activity. A variety of RFM are present across the globe and some can offer low-cost amendments to boost remediation efficiency, while reducing treatment time compared to traditional fertilizer-only methods.

scholarly journals Bioremediation of engine-oil contaminated soil using local residual organic matter

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7389 ◽  
Author(s):  
Kawina Robichaud ◽  
Miriam Lebeau ◽  
Sylvain Martineau ◽  
Marc Amyot
Keyword(s):  
Contaminated Soil ◽  
Treatment Time ◽  
Low Cost ◽  
Carbon Sources ◽  
Engine Oil ◽  
Fertilizer Treatment ◽  
Biolog Ecoplates ◽  
Catabolic Diversity ◽  
Remediation Efficiency ◽  
Spent Grain

Soil remediation industries continue to seek technologies to speed-up treatment and reduce operating costs. Some processes are energy intensive and, in some cases, transport can be the main source of carbon emissions. Residual fertilizing materials (RFM), such as organic residues, have the potential to be beneficial bioremediation agents. Following a circular economy framework, we investigated the feasibility of sourcing RFMs locally to reduce transport and assess possible bioremediation efficiency gains. RFMs were recruited within 100 km of the treatment site: ramial chipped wood (RCW), horse manure (MANR) and brewer spent grain (BSG). They were added to the land treatment unit’s baseline fertilizer treatment (FERT, “F”) to measure if they improved the remediation efficiency of an engine oil-contaminated soil (7,500 ± 100 mg kg−1). Results indicate that MANR-F was the only amendment more effective than FERT for petroleum hydrocarbons (PHC) reduction, while emitting the least CO2 overall. RCW-F was equivalent to FERT but retained more moisture. Although BSG contributed the most nitrogen to the soil, BSG-F retained excessive moisture, emitted more volatile organic compounds, contained less soil O2, and was less effective than the baseline treatment. Significantly more of the C16–C22 fraction was removed (63% ± 22%) than all other fractions (C22–C28, C28–C34, C34–C40), which were equally removed. Microbial community-level physiological profiling was conducted with Biolog Ecoplates™, and catabolic diversity differed between treatments (utilization rates of 31 carbon sources). MANR-F has the potential to increase PHC-remediation speed and efficiency compared to inorganic fertilizer alone. Other RFM promote moisture retention and diverse microbial catabolic activity. A variety of RFM are present across the globe and some can offer low-cost amendments to boost remediation efficiency, while reducing treatment time compared to traditional fertilizer-only methods.

scholarly journals Bioremediation of engine-oil contaminated soil using local residual organic matter

2019 ◽  
Author(s):  
Kawina Robichaud ◽  
Miriam Lebeau ◽  
Sylvain Martineau ◽  
Marc Amyot
Keyword(s):  
Contaminated Soil ◽  
Treatment Time ◽  
Low Cost ◽  
Carbon Sources ◽  
Engine Oil ◽  
Fertilizer Treatment ◽  
Biolog Ecoplates ◽  
Catabolic Diversity ◽  
Remediation Efficiency ◽  
Spent Grain

Soil remediation industries continue to seek technologies to speed-up treatment and reduce operating costs. Some processes are energy intensive and, in some cases, transport can be the main source of carbon emissions. Residual fertilizing matter (RFM), such as organic residues, have the potential to be beneficial bioremediation agents. Following a circular economy framework, we investigated the feasibility of sourcing RFMs locally to reduce transport and assess possible bioremediation efficiency gains. RFMs were recruited within 100 km of the treatment site: ramial chipped wood (RCW), horse manure (MANR) and brewer spent grain (BSG). They were added to the land treatment unit’s baseline fertilizer treatment (FERT, ‘F’) to measure if they improved the remediation efficiency of an engine oil-contaminated soil (7500 ± 100 mg kg-1). Results indicate that MANR-F was the only amendment more effective than FERT for PHC reduction, while emitting the least CO2overall. RCW-F was equivalent to FERT but retained more moisture. Although BSG contributed the most nitrogen to the soil, BSG-F retained excessive moisture, emitted more VOCs, contained less soil O2, and was less effective than the baseline treatment. Significantly more of the C16-C22fraction was removed (63 ± 22%) than all other fractions (C22-C28, C28-C34, C34-C40), which were equally removed. Microbial community-level physiological profiling (CLPP) was conducted with Biolog EcoplatesTM, and catabolic diversity differed between treatments (utilization rates of 31 carbon sources). MANR-F has the potential to increase PHC-remediation speed and efficiency compared to inorganic fertilizer alone. Other RFM promote moisture retention and diverse microbial catabolic activity. A variety of RFM are present across the globe and some can offer low-cost amendments to boost remediation efficiency, while reducing treatment time compared to traditional fertilizer-only methods.

scholarly journals Comparative Studies on the Bioremediation of Used Engine Oil Contaminated Soil using Urea Fertilizer (UF), Goat Manure (GM), Pig Manure (PM) and Brewery Spent Grain (BSG)

2019 ◽  
Vol 3 (1) ◽  
pp. 186-199
Author(s):  
I. R. Ilaboya ◽  
E. A. Otuaro
Keyword(s):  
Linear Regression ◽  
Removal Efficiency ◽  
Contaminated Soil ◽  
Engine Oil ◽  
Pig Manure ◽  
Urea Fertilizer ◽  
Used Engine Oil ◽  
Hydrocarbon Content ◽  
Spent Grain ◽  
Goat Manure

The focus of the study was to evaluate and compare the performance of urea fertilizer, goat manure, pig manure and brewery spent grain for the bioremediation of used engine oil contaminated soil. Soil with no pollution history was collected and subjected to detailed laboratory analysis to determine the total heterotrophic bacterial population, pH, moisture content, total organic carbon, total nitrogen and total hydrocarbon content. Used engine oil contaminated soil was prepared by adding 250g of used engine oil into a clean dry plastic bucket containing 1kg of unpolluted soil. The mixture was properly mixed and covered with aluminium foil paper before use. The used engine oil contaminated soil was left for a period of four (4) days for stabilization before the commencement of treatment. The entire setup and its content was open throughout the period of experimentation to allow for the influence of atmospheric oxidation. The setup was monitored for twelve (12) weeks and sampling/analysis of the samples was done on a weekly basis to ascertain the progress of treatment. The residual hydrocarbon content after each treatment was determined using Atomic Absorption Spectrophotometer. Results obtained shows that pig manure is the best substrate for the clean-up of used engine oil contaminated soil with calculated removal efficiency of 64.4% followed by BSG with 51.4% removal efficiency, Goat Manure with removal efficiency of 39.9% and Urea Fertilizer with 33.7% efficiency. The kinetic modelling shows that experimental data fitted well with pseudo-first order kinetic model with calculated error sum of square (SSE) values of (0.0002, 0.0005, 0.0012 and 0.0072) and root mean square errors (RMSE) of (0.0028, 0.0073, 0.0082 and 0.0481). On the accurate prediction of the optimum remediation time, it was observed that the non-linear regression model gave higher coefficient of determination of 0.9824, 0.9812, 0.9886 and 0.9899 compared to linear regression.

scholarly journals Monitoring the in-situ Bioremediation of Spend engine-oil contaminated soil After Irrigation with Fermented Chicken-droppings

2020 ◽  
Vol 24 (3) ◽  
pp. 411-416
Author(s):  
V.E. Okpashi ◽  
O.A. Ushie ◽  
F.E. Abeng ◽  
I.H. Inyang
Keyword(s):  
Soil Fertility ◽  
Contaminated Soil ◽  
Low Cost ◽  
Positive Control ◽  
Engine Oil ◽  
Control Group ◽  
Used Engine Oil ◽  
Uncontaminated Soil ◽  
Tandem Mass Spectroscopy

Preparation for the bioremediation of petroleum contaminants is explored to provide a low-cost and capable strategy for biodegradation of  contaminants and renewal of soil fertility. In this study, fermented chicken droppings were used as novel in-situ bio-stimulants and bio- augmentation materials. The investigation determines the capability of fermented chicken droppings to biodegrade the residual total petroleum  hydrocarbon compounds in-used engine oil contaminated soil – in the case of auto mechanic shops. The soil was collected at 10 cm depth from the ground, air-dried and sieved with 2.5mm mesh. A 4 kg of soil was weighed into 13 perforated buckets to allow aeration and prevent waterlogging. The setup consists of three replicates that were spiked with 150 ml of used engine oil. 500 ml of the fermented chicken droppings were used to irrigate the contaminated soil at ratio 1:8 periodically for every 3 days per irrigation for 21 days. The uncontaminated soil used as the control group was irrigated with normal water. The TPH composition of the contaminated and remediated soil samples was screened using gas Chromatography tandem mass spectroscopy. Results show that the uncontaminated soil (A-group) had C10 - 24.058 ± 0.02 ppm, C12 - 37.327 ± 0.01 ppm, C14 - 28.515 ± 0.02 ppm and C16 - 12.097 ± 0.02 ppm, respectively out of about 35 TPH compounds that ought to be detected from C8 to C40. The Concentration of TPH in Contaminated soil before irrigation with Chicken droppings – positive control (ppm) B-group gave a significant qualitative and quantitative presence of TPHs in contaminated soil at varying concentrations. 36 TPHs were detected out of forty, starting from C10 - 1.836 ± 0.01 ppm to C38 -  50.150±0.01 ppm. Whereas, the Concentration of residual TPH in Contaminated soil after irrigation with Chicken droppings gave varying levels of residual TPHs ranging from C8 - 1.519 ± 0.02 ppm to C38 - 41.487 ±0.02 ppm. This also gave a resultant/differences in the degradation level of TPHs. Differences in TPH Concentration between before the irrigation and after irrigation of contaminated soil were calculated, C8 had - 0.317 ppm while another TPHs concentration varies accordingly. From the results, one can be deduced that despite other environmental factors that influence the degradation of TPHs, fermented chicken droppings showed great capability in the degradation of TPHs in the contaminated soil. Keywords: Bioremediation, auto-mechanic-yard, Soil-fertility, Used-engine-oil, fermented-chicken-droppings

scholarly journals Effect Of Agitated Chicken-Droppings for in Situ Bioremediation of Pahs in Spend Engine-Oil Contaminated Soil

2021 ◽  
pp. 1-9
Author(s):  
VE Okpashi ◽  
NE Etim ◽  
Inyang IH ◽  
UN Obeten
Keyword(s):  
Contaminated Soil ◽  
Oil Pollution ◽  
Sandy Loam ◽  
Low Cost ◽  
Ground Level ◽  
Positive Control ◽  
Negative Control ◽  
Engine Oil ◽  
Used Engine Oil ◽  
Normal Water

The populace are concern about how their environment is impacted by spend or used engine oil. The spills from spend engine oil posed serious problems to the land and aquatic habitats. Efficient methods but low-cost technique for remediating spends engine oil contaminated soil and restorations of soil fertility are being explored. In response to used engine oil pollution to the soil, the present investigation examined the comparative biodegradation level of spend engine oil contaminated soil using fermented chicken droppings. The soil sample was collected from the uncontaminated site 10 cm below ground level. The sandy/loam soil was sieved with 2.5 mm mesh. A 4 kg of the soil was weighed into each bucket and contaminated with spend engine oil, the soil was contaminated with 150 ml of the spend engine oil at a ratio of 1:27. A 27 kg of chicken droppings was mixed with 27 liters of water in a ratio of 1 kg: 1L. The mixture was left for 7days to ferment and increase the microbial load. The contaminated soil was irrigated using an experimental design that included uncontaminated soil that was irrigated using normal water (positive control), contaminated soil that was irrigated using normal water (negative control) and contaminated soil irrigated using fermented chicken droppings (test). Five hundred mL of the chicken droppings mixture was used to irrigate the soil for 21 days at an interval of 3 days per irrigation. Thereafter, the irrigated soil was screened with gas chromatography linked with mass spectroscopy. There was an observed change in contaminant concentration without fermented chicken droppings. The percentage of differences in the concentrations was recorded in × 10–5% with the presence of Naphthalene and Acenaphthylene with the percent difference of 2.0 × 10–5% and 5.0 × 10– 5%. Result revealed an appreciable decrease in PAHs level compared to the positive and negative control. The findings suggest the positive effect and rapid uptake of the contaminant in the application of fermented chicken droppings. J. Bio-Sci. 29(1): 01-09, 2021 (June)

scholarly journals Burkholderia thailandensis E264 as a promising safe rhamnolipids’ producer towards a sustainable valorization of grape marcs and olive mill pomace

2021 ◽  
Author(s):  
Alif Chebbi ◽  
Massimiliano Tazzari ◽  
Cristiana Rizzi ◽  
Franco Hernan Gomez Tovar ◽  
Sara Villa ◽  
...  
Keyword(s):  
Energy Source ◽  
Olive Oil ◽  
Low Cost ◽  
Carbon Sources ◽  
Burkholderia Thailandensis ◽  
Rhamnolipid Production ◽  
Key Points ◽  
Wide Range ◽  
Long Chain ◽  
Olive Mill

Abstract Within the circular economy framework, our study aims to assess the rhamnolipid production from winery and olive oil residues as low-cost carbon sources by nonpathogenic strains. After evaluating various agricultural residues from those two sectors, Burkholderia thailandensis E264 was found to use the raw soluble fraction of nonfermented (white) grape marcs (NF), as the sole carbon and energy source, and simultaneously, reducing the surface tension to around 35 mN/m. Interestingly, this strain showed a rhamnolipid production up to 1070 mg/L (13.37 mg/g of NF), with a higher purity, on those grape marcs, predominately Rha-Rha C14-C14, in MSM medium. On olive oil residues, the rhamnolipid yield of using olive mill pomace (OMP) at 2% (w/v) was around 300 mg/L (15 mg/g of OMP) with a similar CMC of 500 mg/L. To the best of our knowledge, our study indicated for the first time that a nonpathogenic bacterium is able to produce long-chain rhamnolipids in MSM medium supplemented with winery residues, as sole carbon and energy source. Key points • Winery and olive oil residues are used for producing long-chain rhamnolipids (RLs). • Both higher RL yields and purity were obtained on nonfermented grape marcs as substrates. • Long-chain RLs revealed stabilities over a wide range of pH, temperatures, and salinities

Antioxidant Activity of Peptides Extracted from Brewers’ Spent Grain Peptides

2012 ◽  
Vol 554-556 ◽  
pp. 891-899
Author(s):  
Xu Yan Zong ◽  
Li Li ◽  
Xu Qiao Feng ◽  
Hui Bo Luo ◽  
Jian Zhou ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Nutritive Value ◽  
Superoxide Radical ◽  
Reduced Glutathione ◽  
Protein Hydrolysate ◽  
Low Cost ◽  
Reducing Power ◽  
Gel Permeation Chromatography ◽  
Cosmetic Products ◽  
Spent Grain

Brewer’s spent grain (BSG) protein extracted from BSG was hydrolyzed using Alcalase to produce BSG protein hydrolysate. BSG protein hydrolysate was fractionated by ultrafiltration to obtain brown color BSG peptides. Antioxidant activity of BSG peptides was analyzeded and compared with reduced glutathione (GSH). BSG peptides exhibited 50% of scavenging activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and superoxide radical with concentrations less than 0.8 mg/mL, 0.6 mg/mL and 0.6 mg/mL, respectively. The reducing power of BSG peptides was 0.70 at the concentration of 2.00 mg/mL. 86.30% of the total amount of the BSG peptides purified by gel permeation chromatography was below 2000 Da. Because of its antioxidant activity, stability, nutritive value and low cost, BSG peptides exerts a possibility to use in food or cosmetic products.

scholarly journals Analysis of Drying of Brewers’ Spent Grain

Proceedings ◽  
2018 ◽  
Vol 2 (23) ◽  
pp. 1467 ◽  
Author(s):  
José Ignacio Arranz ◽  
María Teresa Miranda ◽  
Francisco José Sepúlveda ◽  
Irene Montero ◽  
Carmen Victoria Rojas
Keyword(s):  
Energy Use ◽  
Low Cost ◽  
Matter Content ◽  
Raw Material ◽  
Dry Matter Content ◽  
Brewing Industry ◽  
Livestock Feed ◽  
Spent Grain ◽  
Very High ◽  
Animal Consumption

Brewing industry generates a main residue, brewers’ spent grain (BSG), which has good properties both for use in animal consumption and for thermal use, but contains a very high content of moisture (20–25% dry matter content), so that its elimination or treatment should be immediate, since it can cause degeneration problems of the product. Currently, brewers often supply this material at low cost for use as livestock feed. This solution is not efficiently carried out without reporting too much benefit to the brewers more than to eliminate waste from their facilities. However, BSG is a raw material of interest for application in different areas due to its low price, availability throughout the year and a valuable chemical composition, so it seems necessary to look for an alternative use to give value to these characteristics. In this paper a drying study is carried out in order to establish the foundations for its energy use by thermal of BSG. BSG has been used from a craft brewery located at Badajoz, Spain. Drying analysis was carried out for various temperatures and inlet air flow by means a convective dryer. The properties studied show that BSG can be used for thermal utilization in large installations, being necessary heat drying processes as a pretreatment in order to obtain a biofuel with acceptable efficiency.

Sign in / Sign up

Export Citation Format

Share Document