Isolation and Identification of Fungi Associated with Habanero Pepper (Capsicum chinense jacq) Using Basic Molecular Techniques

Habanero pepper (Capsicum chinense Jacq.) is an economically important vegetable. It is used as spices for foods and for medicinal purposes in many parts of the country. Despite the importance of the crop, it has been observed that during storage, the fruits of the Habanero pepper (HP) shelf-life is reduced by several post-harvest diseases caused by fungal pathogens. A study was conducted to identify the common fungal pathogens causing post-harvest rot of Habanero fruits at the daily market in Omoko Aluu Town, Rivers State. The DNA of the most common fungal isolate HP-02 was molecularly characterized using Internal Transcribed Spacer 1 (ITS-1) molecular marker. The HP-02 isolate DNA sequence was aligned using the Basic Local Alignment Search Tool for nucleotide (BLASTN) 2.8.0 version of the National Center for Biotechnology Information (NCBI) database. The results indicated that the HP-02 isolate sequence was 79% identical to Mucor irregularis and also Rhizomucor viriabilis isolate SAPB3. These findings showed that Mucor irregularis, which was formerly known as Rhizomucor variabilis is one of the emerging causal fungal pathogens of post-harvest Habanero pepper.

Download Full-text

Molecular characterization of fungi associated with stored soybean (Glycine max L) seeds

Nigerian Journal of Biotechnology ◽

10.4314/njb.v36i2.4 ◽

2020 ◽

Vol 36 (2) ◽

pp. 27-32

Author(s):

C.G. Ikechi-Nwogu ◽

F.C. Okene

Keyword(s):

Fungal Pathogens ◽

Fungal Species ◽

Molecular Techniques ◽

Local Alignment ◽

Post Harvest ◽

High Quality Protein ◽

Search Tool ◽

Food And Feed ◽

Glycine Max L

Soybean is an important legume that has high quality protein and oil for food and feed. Despite the importance of this legume, the crop is affected by several post-harvest diseases caused by fungi. A study was carried out to identify the fungal species associated with the seeds of soybean using molecular techniques. The DNA of the isolate, was molecularly characterized using Internal Transcribed Spacer 1 (ITS-1) molecular marker. The isolate DNA sequence, was aligned using the Basic Local Alignment Search Tool for nucleotide (BLASTN) 2.8.0 version of the National Center for Biotechnology Information (NCBI) database. The results showed that the isolate sequence was 98% identical to Diaporthe spp. Voucher VP51, 98% identical to Diaporthe schini isolate L5N71 and 98% identical to Diaporthe schini strain B125. These findings showed that Diaporthe spp. is one of the causal fungal pathogens of post-harvest diseases of soybean seeds. It is anticipated that these results will provide information on culturing Diaporthe species also provide the basis for further study to show their antibiotic and anti-cancerous, enzymes and secondary metabolites producing ability. Keywords: Soybean, Diaporthe schini and RBCL marker

Download Full-text

Isolation and Identification of a Volatile Compound in Habanero Pepper (Capsicum chinense)

Journal of Oleo Science ◽

10.5650/jos.ess18055 ◽

2018 ◽

Vol 67 (10) ◽

pp. 1219-1225 ◽

Cited By ~ 1

Author(s):

Yusuke Murakami ◽

Hisakatsu Iwabuchi ◽

Manabu Horikawa ◽

Shoko Mori ◽

Yukie Ohba ◽

...

Keyword(s):

Volatile Compound ◽

Capsicum Chinense ◽

Isolation And Identification ◽

Habanero Pepper

Download Full-text

Isolation and Identification of Fungal Pathogen Associated with Post Harvest Deterioration of Cucumber (Cucumis sativus L.) Fruits in Three Selected Markets in Jos, Nigeria

International Journal of Plant & Soil Science ◽

10.9734/ijpss/2019/v30i630196 ◽

2019 ◽

pp. 1-8

Author(s):

M. Ishaya ◽

A. E. Anzaku ◽

W. C. John ◽

N. Janfa ◽

O. Oke ◽

...

Keyword(s):

Fungal Pathogens ◽

Sampling Technique ◽

Pathogenic Fungi ◽

Geotrichum Candidum ◽

Isolation And Identification ◽

Cucumis Sativus L ◽

Post Harvest ◽

Fungal Isolates ◽

Cucumber Fruit ◽

Fruit Samples

Aim: This study was carried out to isolate, identify and characterize fungal pathogens associated with post-harvest spoilage of cucumber. Study Design: This research study was done using random sampling technique. Place and Duration of Study: The experiment was carried out from March to June, 2019 at biology laboratory Federal College of Forestry, Jos. Methodology: Fungi isolates were obtained from diseased portions of the cucumber fruit samples cultured on PDA media and incubated at 28ºC2 for seven days. The mycelial were identified macroscopically and microscopically. Pathogenicity tests were conducted for all the fungal pathogens identified by inoculating healthy cucumber samples and incubating for ten days with the readings being taken at two day intervals. The data obtained were analyzed using ANOVA and means were separated using LSD at P ≤ 0.05. Results: Aspergillus fumigatus, Fusarium sp., Geotrichum candidum and Yeast sp were isolated and identified. Result showed that Gada biu market was the most heavily infested location with all the fungal isolates. Geotrichum candidum constituted the highest (50%) occurrence of fungal isolates from all locations. All the fungal isolates were found to be pathogenic on cucumber fruits, with Fusarium being the most destructive, followed by yeast and Geotrichum candidum and the least was Aspergilus fumigatus. Conclusion: The results obtained in this study showed the isolates identified were involved in Cucumber spoilage. Therefore, Careful handling of Cucumber fruits should be ensured to prevent the spread of these pathogenic fungi.

Download Full-text

Molecular Characterization of Post Harvest Fungus Associated with Spoilt Broccoli

Asian Journal of Biology ◽

10.9734/ajob/2021/v13i330189 ◽

2021 ◽

pp. 47-51

Author(s):

Ikechi – Nwogu, Chinyerum Gloria ◽

B. A. Odogwu ◽

O. G. Obiakoeze

Keyword(s):

Aspergillus Niger ◽

Fungal Pathogens ◽

Sequence Similarity ◽

Local Alignment ◽

Control Approach ◽

Post Harvest ◽

Morphological Studies ◽

Search Tool ◽

Fungal Organisms ◽

Post Harvest Deterioration

Broccoli (Brassica oleracea var. italica) is a nutritional vegetable that looks like a small tree. Despite the fact that it is extensively loaded with arrays of vitamins, minerals, fiber and antioxidants, it has been observed that it has a short lifespan of not more than 2-5 days due to post-harvest deterioration. A study was conducted to isolate and identify the common fungal pathogens causing post-harvest deterioration of broccoli crown. Diseased broccoli crowns were collected from Ogunabali Fruit Garden Market in D-Line, Port Harcourt Local Government Area of Rivers State. Fungal isolates were collected and morphologically identified. The DNA of the most common fungal isolate, BC-3B was molecularly characterized using Internal Transcribed Spacer 4 and 5 (ITS-4 and 5) molecular markers. The morphological studies revealed that the BC-3B isolate was an Aspergillus niger. The BC-3B isolate DNA sequence was aligned using Basic Local Alignment Search Tool for Nucleotide (BLASTN) 2.8.0 version of National Center for Biotechnology Information (NCBI) database. The molecular weight of the DNA of the isolates was over 600base pairs. Based on sequence similarity, it was observed that the broccoli isolate BC-3B was 93% identical to Aspergillus niger. From the above results, these findings showed that Aspergillus niger is the causal fungal pathogen of post-harvest rot of broccoli. Phylogenetic tree was constructed to access the relationship between the isolates obtained from this study. This study has provided information on some of the fungal organisms found in broccoli. It is anticipated that this result will provide information for disease control approach for alleviating the post-harvest losses of broccoli caused by Aspergillus niger and provide a foundation for further study of possible harm of consuming diseased broccoli.

Download Full-text

Isolation and identification of fungi associated with the spoilage of sweet orange (Citrus sinensis L) and banana (Musa sapientum L) in Sokoto Metropolis

Journal of Applied Biotechnology & Bioengineering ◽

10.15406/jabb.2018.05.00135 ◽

2018 ◽

Vol 5 (3) ◽

Author(s):

Muhammad AS ◽

Mohammed IU ◽

Ameh M ◽

Bello I ◽

Haliru BS ◽

...

Keyword(s):

Citrus Sinensis ◽

Sweet Orange ◽

Isolation And Identification ◽

Musa Sapientum ◽

Identification Of Fungi

Download Full-text

Diagnostic Accuracy of a Direct Panfungal Polymerase Chain Reaction Assay Performed on Stained Cytology Slides

Veterinary Pathology ◽

10.1177/0300985821991562 ◽

2021 ◽

pp. 030098582199156

Author(s):

Alexandra N. Myers ◽

Unity Jeffery ◽

Zachary G. Seyler ◽

Sara D. Lawhon ◽

Aline Rodrigues Hoffmann

Keyword(s):

Polymerase Chain Reaction ◽

Diagnostic Accuracy ◽

Molecular Techniques ◽

Fungal Culture ◽

Chain Reaction ◽

Identification Of Fungi ◽

Fungal Dna ◽

Polymerase Chain ◽

Panfungal Pcr ◽

Pcr Targeting

Molecular techniques are increasingly being applied to stained cytology slides for the diagnosis of neoplastic and infectious diseases. Such techniques for the identification of fungi from stained cytology slides have not yet been evaluated. This study aimed to assess the diagnostic accuracy of direct (without nucleic acid isolation) panfungal polymerase chain reaction (PCR) followed by sequencing for identification of fungi and oomycetes on stained cytology slides from dogs, cats, horses, and other species. Thirty-six cases were identified with cytologically identifiable fungi/oomycetes and concurrent identification via fungal culture or immunoassay. Twenty-nine controls were identified with no cytologically or histologically visible organisms and a concurrent negative fungal culture. Direct PCR targeting the internal transcribed spacer region followed by sequencing was performed on one cytology slide from each case and control, and the sensitivity and specificity of the assay were calculated. The sensitivity of the panfungal PCR assay performed on stained cytology slides was 67% overall, 73% excluding cases with oomycetes, and 86% when considering only slides with abundant fungi. The specificity was 62%, which was attributed to amplification of fungal DNA from control slides with no visible fungus and negative culture results. Direct panfungal PCR is capable of providing genus- or species-level identification of fungi from stained cytology slides. Given the potential of panfungal PCR to amplify contaminant fungal DNA, this assay should be performed on slides with visible fungi and interpreted in conjunction with morphologic assessment by a clinical pathologist.

Download Full-text