scholarly journals In vitro effect of lysophosphatidic acid on proliferation, invasion and migration of human ovarian cancer cells

2018 ◽  
Vol 17 (2) ◽  
pp. 219
Author(s):  
Qingfu Wang ◽  
Lixin Zhu ◽  
Aiping Qin ◽  
Tiefeng Chen ◽  
Jinpen Li ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Lysophosphatidic Acid ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Invasion And Migration ◽  
And Migration

scholarly journals Long non-coding RNA XIST regulates ovarian cancer progression via modulating miR-335/BCL2L2 axis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Qingjuan Meng ◽  
Ningning Wang ◽  
Guanglan Duan
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Invasion And Migration ◽  
Non Coding Rna ◽  
And Migration ◽  
Long Non Coding Rna

Abstract Background X inactivation-specific transcript (XIST) is the long non-coding RNA (lncRNA) related to cancer, which is involved in the development and progression of various types of tumor. However, up to now, the exact role and molecular mechanism of XIST in the progression of ovarian cancer are not clear. We studied the function of XIST in ovarian cancer cells and clinical tumor specimens. Methods RT-qPCR was performed to detect the expression levels of miR-335 and BCL2L2 in ovarian cancer cells and tissues. MTT and transwell assays were carried out to detect cell proliferation, migration, and invasion abilities. Western blot was performed to analyze the expression level of BCL2L2. The interaction between miR-335 and XIST/BCL2L2 was confirmed using a luciferase reporter assay. Results The inhibition of XIST can inhibit the proliferation invasion and migration of human ovarian cancer cells. In addition, the miR-335/BCL2L2 axis was involved in the functions of XIST in ovarian cancer cells. These results suggested that XIST could regulate tumor proliferation and invasion and migration via modulating miR-335/BCL2L2. Conclusion XIST might be a carcinogenic lncRNA in ovarian cancer by regulating miR-335, and it can serve as a therapeutic target in human ovarian cancer.

scholarly journals microRNA-4488 in extracellular vesicles derived from marrow mesenchymal stem cells suppresses ABHD8 to inhibit ovarian cancer progression

2020 ◽  
Author(s):  
Lei Chang ◽  
Junying Zhou ◽  
Wanjia Tian ◽  
Mengyu Chen ◽  
Ruixia Guo ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Proliferation ◽  
Cancer Cells ◽  
Cancer Progression ◽  
Luciferase Reporter ◽  
Ovarian Cancer Cells ◽  
Invasion And Migration ◽  
And Migration

Abstract Background Extracellular vesicle (EV) that delivered microRNAs (miRNAs) have been found as the important biomarkers participating in the pathological mechanism of ovarian cancer. Consequently, this study sought to examine the underlying mechanism of mesenchymal stem cell (MSC)-derived EVs containing miR-4488 in ovarian cancer. Methods The normal ovarian tissues and ovarian cancer tissues were extracted, and the information of MSC-EV miRNA was obtained by Bioinformatics analysis. RT-qPCR and western blot analysis were applied to detect miR-4488 and α/β-hydrolase domain-containing (ABHD)8 expression followed by determination of relationship between miR-4488 and ABHD8 by dual-luciferase reporter assay. After transfection with different plasmids and treatment with DMSO or GW4869 (inhibitor of EV), the regulatory roles of MSC-EV-miR-4488 in invasion, proliferation, apoptosis, and migration of cancer cells were explored. Besides, xenograft tumor in nude mice was conducted to explore the role of miR-4488 and ABHD8 in ovarian cancer in vivo. Results miR-4488 was poorly expressed and ABHD8 was highly expressed in ovarian cancer cells and tissues. ABHD8 was a target gene of miR-4488 while the knockdown of ABHD8 resulted in the suppression of proliferation, invasion, and migration while promoting the apoptosis of cancer cells. Functionally, MSC-EV-derived miR-4488 inhibited the expression of ABHD8. Additionally, miR-4488 over-expressed in MSC-EVs inhibited the cell proliferation, invasion, and migration through down-regulation of ABHD8 expression. At last, these in vitro findings were also confirmed in vivo. Conclusion To summarize, miR-4488 overexpressed in MSC-EVs suppressed ABHD8 expression to inhibit the cancer cell proliferation, invasion, and migration, thus suppressing ovarian cancer.

scholarly journals E-cadherin promotes proliferation of human ovarian cancer cells in vitro via activating MEK/ERK pathway

2012 ◽  
Vol 33 (6) ◽  
pp. 817-822 ◽  
Author(s):  
Ling-ling Dong ◽  
Lian Liu ◽  
Chun-hong Ma ◽  
Ji-sheng Li ◽  
Chao Du ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Erk Pathway ◽  
E Cadherin

Heterogeneous interaction of interferon-β and taxol in human ovarian cancer cells in vitro

1994 ◽  
Vol 30 (6) ◽  
pp. 892-893
Author(s):  
S. Sen ◽  
A. Zocchetti ◽  
P. Beccaglia ◽  
G. Balconi ◽  
E. Erba ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Interferon Β ◽  
Heterogeneous Interaction

569 Synergistic effect of cisplatin combination with indole-3-ethyl isothiocyanate on proliferation of human ovarian cancer cells in vitro

2010 ◽  
Vol 8 (5) ◽  
pp. 145
Author(s):  
L. Hunakova ◽  
P. Stehlik ◽  
H. Paulikova ◽  
P. Gronesova ◽  
M. Pastorek ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Synergistic Effect ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer

scholarly journals Antitumor properties of salinomycin on cisplatin-resistant human ovarian cancer cells in vitro and in vivo: Involvement of p38 MAPK activation

2013 ◽  
Vol 29 (4) ◽  
pp. 1371-1378 ◽  
Author(s):  
BEI ZHANG ◽  
XUEYA WANG ◽  
FENGFENG CAI ◽  
WEIJIE CHEN ◽  
ULI LOESCH ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
P38 Mapk ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Mapk Activation ◽  
Antitumor Properties

In vitro metastatic colonization of human ovarian cancer cells to the omentum

2010 ◽  
Vol 27 (3) ◽  
pp. 185-196 ◽  
Author(s):  
Shaheena M. Khan ◽  
Holly M. Funk ◽  
Sophie Thiolloy ◽  
Tamara L. Lotan ◽  
Jonathan Hickson ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Metastatic Colonization
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