Extracts of edible, medicinal Thai plants inhibit the human breast cancer cells

Purpose: To evaluate the effects of ten edible, medicinal Thai plant extracts on MCF-7 cell viability and cell migration, as well as their mechanism(s) of action. Methods: Ethanolic plant extracts of ten edible, medicinal plants were tested for their cytotoxicity against MCF-7 cells using sulforhodamine B (SRB). To investigate the cytotoxic mechanism(s) of action of these extracts, the study was examined gene expression and protein expression by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Cell migration was studied by wound healing assay. Results: Four of the ten test extracts were potently cytotoxic, Careya sphaerica (CS), Azadirachta indica (AI), Piper nigrum (PN) and Oroxylum indicum (OI) with half maximal inhibitory concentrations (IC50) less than 100 μg/mL. All four extracts stimulated ROS overgeneration, increased caspase 3 activity and decreased growth-related gene expression including cdk2, cdk4, cdk6, cyclin D1 and cyclin E. Furthermore, the extracts significantly enhanced cyclin-dependent kinase inhibitor (CDKI) p21 levels and activated cancer cell death. The four extracts, CS, AI, PN and OI, also significantly reduced cancer cell migration, with PN being the most potent. Conclusion: Extract of the edible plants CS, AI, PN and OI have in vitro anticancer activity and are promising starting points for the development of breast cancer drugs. Keywords: Careya sphaerica (CS), Azadirachta indica (AI), Piper nigrum (PN), Oroxylum indicum (OI), Breast cancer, Cell death

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Effects of protein kinase Cδ and phospholipase C-γ1 on monocyte chemoattractant protein-1 expression in taxol-induced breast cancer cell death

International Journal of Molecular Medicine ◽

10.3892/ijmm_00000303 ◽

2009 ◽

Vol 24 (06) ◽

Cited By ~ 1

Author(s):

Keyword(s):

Breast Cancer ◽

Cell Death ◽

Protein Kinase ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Monocyte Chemoattractant Protein 1 ◽

Monocyte Chemoattractant ◽

Monocyte Chemoattractant Protein ◽

Cancer Cell Death ◽

Protein Kinase Cδ

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Calcitriol and menadione produces cell cycle arrest, oxidative and nitrosative stress, mitochondrial damage and cell death on MCF-7 breast cancer cell

Bone ◽

10.1016/j.bone.2015.12.032 ◽

2016 ◽

Vol 89 ◽

pp. 67

Author(s):

S. Guizzardi ◽

G. Picotto ◽

V. Rodríguez ◽

L. Bohl ◽

N. Tolosa de Talamoni

Keyword(s):

Breast Cancer ◽

Cell Cycle ◽

Cell Death ◽

Cell Cycle Arrest ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Nitrosative Stress ◽

Oxidative And Nitrosative Stress ◽

Cycle Arrest ◽

Mcf 7

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Factors regulating pS2-reporter gene expression in MCF-7 breast cancer cell line

International Journal of Oncology ◽

10.3892/ijo.10.5.1051 ◽

1997 ◽

Author(s):

S Beck ◽

P Fegert ◽

P Gott

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Cell Line ◽

Reporter Gene ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Breast Cancer Cell Line ◽

Cancer Cell Line ◽

Reporter Gene Expression ◽

Mcf 7

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The potential utility of acetyltanshinone IIA in the treatment of HER2-overexpressed breast cancer: Induction of cancer cell death by targeting apoptotic and metabolic signaling pathways

Oncotarget ◽

10.18632/oncotarget.4156 ◽

2015 ◽

Vol 6 (26) ◽

pp. 21865-21877 ◽

Cited By ~ 16

Author(s):

Mounia Guerram ◽

Zhen-Zhou Jiang ◽

Bashir Alsiddig Yousef ◽

Aida Mejda Hamdi ◽

Hozeifa Mohamed Hassan ◽

...

Keyword(s):

Breast Cancer ◽

Cell Death ◽

Signaling Pathways ◽

Cancer Cell ◽

Potential Utility ◽

Metabolic Signaling ◽

Cancer Induction ◽

Cancer Cell Death

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PMCA2 silencing potentiates MDA-MB-231 breast cancer cell death initiated with the Bcl-2 inhibitor ABT-263

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2016.09.030 ◽

2016 ◽

Vol 478 (4) ◽

pp. 1792-1797 ◽

Cited By ~ 11

Author(s):

Merril Curry ◽

Sarah J. Roberts-Thomson ◽

Gregory R. Monteith

Keyword(s):

Breast Cancer ◽

Cell Death ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Cancer Cell Death

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Garcinia morella fruit, a promising source of antioxidant and anti-inflammatory agents induces breast cancer cell death via triggering apoptotic pathway

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2018.04.068 ◽

2018 ◽

Vol 103 ◽

pp. 562-573 ◽

Cited By ~ 13

Author(s):

Bhaswati Choudhury ◽

Raghuram Kandimalla ◽

R. Elancheran ◽

Rupjyoti Bharali ◽

Jibon Kotoky

Keyword(s):

Breast Cancer ◽

Cell Death ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Apoptotic Pathway ◽

Cancer Cell Death ◽

Anti Inflammatory ◽

Promising Source

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Enhancement of paclitaxel-induced breast cancer cell death via the glycogen synthase kinase-3β-mediated B-cell lymphoma 2 regulation

BMB Reports ◽

10.5483/bmbrep.2016.49.1.102 ◽

2016 ◽

Vol 49 (1) ◽

pp. 51-56 ◽

Cited By ~ 1

Author(s):

Kyung Tae Noh ◽

Gil Sun Cha ◽

Tae Heung Kang ◽

Joon Cho ◽

In Duk Jung ◽

...

Keyword(s):

Breast Cancer ◽

Cell Death ◽

B Cell ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Cell Lymphoma ◽

Glycogen Synthase ◽

B Cell Lymphoma ◽

Glycogen Synthase Kinase 3Β ◽

Cancer Cell Death

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Potent Cytotoxicity of Four Cameroonian Plant Extracts on Different Cancer Cell Lines

Pharmaceuticals ◽

10.3390/ph13110357 ◽

2020 ◽

Vol 13 (11) ◽

pp. 357

Author(s):

Ahmed Somaida ◽

Imran Tariq ◽

Ghazala Ambreen ◽

Ahmed Mohamed Abdelsalam ◽

Abdallah Mohamed Ayoub ◽

...

Keyword(s):

Cell Death ◽

Cell Migration ◽

Cell Lines ◽

Cancer Cell ◽

Plant Extracts ◽

Caspase 3 ◽

Cancer Cell Lines ◽

Imperata Cylindrica ◽

Tumor Cell Death ◽

Genetic Toxicity

In this study, the potential cytotoxicity of four plant extracts originated from Cameroon: Xylopia aethiopica (XA), Imperata cylindrica (IC), Echinops giganteus (EG) and Dorstenia psilurus (DP) were examined in vitro. We tested the anti-proliferative activity of the methanolic extracts of these compounds using MTT assay on seven different human cancer cell lines: HeLa, MDA-MB-231, A549, HepG2, U-87, SK-OV-3 and HL60. Induction of cell death was assessed by cell cycle analysis, apoptosis was determined by Annexin V-FITC binding and caspase 3/7 activity. As well, changes in mitochondrial membrane potential (MMP) and cell migration were tested. The genetic toxicity, using the alkaline comet assay, was evaluated. The studied extracts inhibited the cell proliferation of all tested cancer cell lines with concentration dependent effect over time. All of these extracts mainly induced apoptosis of HeLa cells by the accumulation of hypodiploid cells in the sub-G0/G1 phase and increasing the activity of caspase 3/7, as well they showed potential MMP disturbance and expressed a marked inhibitory effect on cell migration. Assessment of probable genetic toxicity by these extracts revealed no or minimum incidence of genetic toxicity. Therefore, the studied plant extracts are exhibiting potent anticancer activity based upon marked induction of tumor-cell death.

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