scholarly journals Increasing Mucuna's potential as a food and feed crop: An effort to deliver BNF to farmers

2009 ◽  
Vol 6 (1) ◽  
Author(s):  
RJ Carsky ◽  
M Ellitta
Keyword(s):  

2015 ◽  
Vol 134 (2) ◽  
pp. 135-147 ◽  
Author(s):  
Salej Sood ◽  
Rajesh K. Khulbe ◽  
Arun K. Gupta ◽  
Pawan K. Agrawal ◽  
Hari D. Upadhyaya ◽  
...  




Amaranth is a valuable food and feed crop that can solve the problem of vegetable protein in animal husbandry. The aim of the research was to study elements of amaranth cultivation technology: the dose of nitrogen fertilizers (without fertilizers, N15, N20, N25) and the row spacing (15, 30, 45 cm). The work was carried out on sod-podzolic medium loamy gleyic soil on the experimental field of FSBEI HE Yaroslavl State Agricultural Academy in 2018. The studied parameters were determined according to generally ac-cepted methods. The use of nitrogen fertilizers contributed to a significant increase in leaf area and pho-tosynthetic potential of amaranth crops at the highest values of the background N20 – 4.7 thousand m2 / ha and 324.08 thousand m2 / ha × days, respectively. Increase in row spacing when sowing amaranth raised the leaf surface area value, accumulation of dry aboveground mass, photosynthetic potential, ger-mination and safety at maximum values in the variant with a row spacing of 45 cm. abundance of weed flea, cereal aphid and sunflower fire. Sowing amaranth with a width of 45 cm between rows caused a sta-tistically significant decrease in the number of studied pests, as well as the number and dry weight of young and perennial weeds. The use of nitrogen fertilizers in a dose of 25 kg AI / ha contributed to a sig-nificant decrease in the yield of amaranth green mass from 145.12 q / ha on the «no-fertilizer» variant to 127.93 q / ha, with maximum values for the background N20 – 156, 55 kg / ha. Thus, the greatest effi- 128 ciency was shown by the introduction of nitrogen fertilizers at a dose of 20 kg ai / ha when sown with a row spacing of 45 cm.



2010 ◽  
Vol 2 (2) ◽  
pp. 140-153 ◽  
Author(s):  
Laura Anfossi ◽  
Claudio Baggiani ◽  
Cristina Giovannoli ◽  
Gianfranco Giraudi


2021 ◽  
Vol 22 (2) ◽  
pp. 480
Author(s):  
Jongrae Kim ◽  
Kwang Suk Chang ◽  
Sangmuk Lee ◽  
EonSeon Jin

To date, Chlorella vulgaris is the most used species of microalgae in the food and feed additive industries, and also considered as a feasible cell factory for bioproducts. However, the lack of an efficient genetic engineering tool makes it difficult to improve the physiological characteristics of this species. Therefore, the development of new strategic approaches such as genome editing is trying to overcome this hurdle in many research groups. In this study, the possibility of editing the genome of C. vulgaris UTEX395 using clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) has been proven to target nitrate reductase (NR) and adenine phosphoribosyltransferase (APT). Genome-edited mutants, nr and apt, were generated by a DNA-mediated and/or ribonucleoprotein (RNP)-mediated CRISPR-Cas9 system, and isolated based on the negative selection against potassium chlorate or 2-fluoroadenine in place of antibiotics. The null mutation of edited genes was demonstrated by the expression level of the correspondent proteins or the mutation of transcripts, and through growth analysis under specific nutrient conditions. In conclusion, this study offers relevant empirical evidence of the possibility of genome editing in C. vulgaris UTEX395 by CRISPR-Cas9 and the practical methods. Additionally, among the generated mutants, nr can provide an easier screening strategy during DNA transformation than the use of antibiotics owing to their auxotrophic characteristics. These results will be a cornerstone for further advancement of the genetics of C. vulgaris.



ACS Omega ◽  
2020 ◽  
Vol 5 (51) ◽  
pp. 33242-33252
Author(s):  
Marja Nappa ◽  
Michael Lienemann ◽  
Camilla Tossi ◽  
Peter Blomberg ◽  
Jussi Jäntti ◽  
...  


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