scholarly journals Abundance of pathogenic bacteria and viral indicators in chlorinated effluents produced by four wastewater treatment plants in the Gauteng Province, South Africa

Water SA ◽  
2010 ◽  
Vol 36 (5) ◽  
Author(s):  
M Dungeni ◽  
R.R Van der Merwe ◽  
M Momba
Keyword(s):  
South Africa ◽  
Wastewater Treatment ◽  
Pathogenic Bacteria ◽  
Wastewater Treatment Plants ◽  
Gauteng Province ◽  
Viral Indicators

The occurrence of opioid compounds in wastewater treatment plants and their receiving water bodies in Gauteng province, South Africa

2021 ◽  
pp. 118048
Author(s):  
Ilunga Kamika ◽  
Shohreh Azizi ◽  
Adolph A. Muleja ◽  
Ramganesh Selvarajan ◽  
Mohamed Azab El-Liethy ◽  
...  
Keyword(s):  
South Africa ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Water Bodies ◽  
Gauteng Province ◽  
Receiving Water

scholarly journals Bacteria from the Genus Arcobacter Are Abundant in Effluent from Wastewater Treatment Plants

2020 ◽  
Vol 86 (9) ◽  
Author(s):  
Jannie Munk Kristensen ◽  
Marta Nierychlo ◽  
Mads Albertsen ◽  
Per Halkjær Nielsen
Keyword(s):  
Wastewater Treatment ◽  
Pathogenic Bacteria ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Amplicon Sequencing ◽  
Rrna Gene ◽  
Content Type ◽  
Effluent Quality ◽  
Culture Independent ◽  
Influent Wastewater

ABSTRACT Pathogenic bacteria in wastewater are generally considered to be efficiently removed in biological wastewater treatment plants. This understanding is almost solely based on culture-based control measures, and here we show, by applying culture-independent methods, that the removal of species in the genus Arcobacter was less effective than for many other abundant genera in the influent wastewater. Arcobacter was one of the most abundant genera in influent wastewater at 14 municipal wastewater treatment plants and was also abundant in the “clean” effluent from all the plants, reaching up to 30% of all bacteria as analyzed by 16S rRNA gene amplicon sequencing. Metagenomic analyses, culturing, genome sequencing of Arcobacter isolates, and visualization by fluorescent in situ hybridization (FISH) confirmed the presence of the human-pathogenic Arcobacter cryaerophilus and A. butzleri in both influent and effluent. The main reason for the high relative abundance in the effluent was probably that Arcobacter cells, compared to those of other abundant genera in the influent, did not flocculate and attach well to the activated sludge flocs, leaving a relatively large fraction dispersed in the water phase. The study shows there is an urgent need for new standardized culture-independent measurements of pathogens in effluent wastewaters, e.g., amplicon sequencing, and an investigation of the problem on a global scale to quantify the risk for humans and livestock. IMPORTANCE The genus Arcobacter was unexpectedly abundant in the effluent from 14 Danish wastewater treatment plants treating municipal wastewater, and the species included the human-pathogenic A. cryaerophilus and A. butzleri. Recent studies have shown that Arcobacter is common in wastewater worldwide, so the study indicates that discharge of members of the genus Arcobacter may be a global problem, and further studies are needed to quantify the risk and potentially minimize the discharge. The study also shows that culture-based analyses are insufficient for proper effluent quality control, and new standardized culture-independent measurements of effluent quality encompassing most pathogens should be considered.

scholarly journals Isolation and antibiotic profile ofVibriospp. in final effluents of two wastewater treatment plants in the Eastern Cape of South Africa

2018 ◽  
Author(s):  
Olayinka Osuolale ◽  
Anthony Okoh
Keyword(s):  
South Africa ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Treated Wastewater ◽  
Eastern Cape ◽  
Sources Of Pollution ◽  
Antibiotic Drugs ◽  
Treated Effluents ◽  
Vibrio Spp

ABSTRACTBackgroundPoorly or partially treated wastewater disposed of can contaminate water and even properly treated sewage can have its problems. The highlight of this danger is wastewater treatment plants serving as reservoir for proliferation of antibiotic resistant organisms. We have reported the state of two wastewater treatment in the Eastern Cape of South Africa which discharge poorly and partially treated effluents. Our aims to identify Vibrio spp. and their antibiotic profiles in treated final effluent discharge from wastewater treatment plant.MethodsCulture based approach using the TCBS agar for isolationVibriospp., presumptive isolates were purified and confirmed using PCR. The confirmed isolated were also genotyped to identify the species present. The antibiotic profiling of the confirmed isolates was using the CLSI recommended first line antibiotics for Vibrio.ResultsOut of the 786 presumptive isolates, 374 were confirmed asVibriospp. None of the Vibrio spp. pathotypes were present in the confirmed isolates. Randomized isolates of 100 Vibrio spp. were selected, > 90 % of the isolates were susceptible to Ciprofloxacin, and > 50 – 80 % for Ampicillin, Chloramphenicol, Tetracycline, Cefotaxime, and Trimethoprim-sulfamethoxazole respectively.ConclusionsWe are able to isolate Vibrio spp. from treated effluents but none of their pathotypes were present. The antibiotic agents considered for primary testing which are ciprofloxacin was the most effective of the antibiotic drugs, followed by cefotaxime, tetracycline with less susceptibility. Contamination from discharged effluents from wastewater treatment can lead to spread of spread of disease in this environment. The WWTPs studied are sources of pollution to surface water with environmental and public health.

scholarly journals Reduction of Arcobacter at Two Conventional Wastewater Treatment Plants in Southern Arizona, USA

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 175 ◽  
Author(s):  
Ghaju Shrestha ◽  
Sherchan ◽  
Kitajima ◽  
Tanaka ◽  
Gerba ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Quantitative Pcr ◽  
Pathogenic Bacteria ◽  
Wastewater Treatment Plants ◽  
The United States ◽  
Rrna Gene ◽  
Metagenomic Sequencing ◽  
Wwtp Effluent

This study aimed to identify the bacterial community in two wastewater treatment plants (WWTPs) and to determine the occurrence and reduction of Arcobacter, along with virulence genes (ciaB and pldA). A total of 48 samples (24 influent and 24 effluent) were collected at two WWTPs in southern Arizona in the United States, monthly from August 2011 to July 2012. Bacterial DNA extract was utilized for 16S rRNA metagenomic sequencing. Quantification of Arcobacter 16S rRNA gene was conducted using a recently developed SYBR Green-based quantitative PCR assay. Among 847 genera identified, 113 (13%) were identified as potentially pathogenic bacteria. Arcobacter 16S rRNA gene was detected in all influent samples and ten (83%) and nine (75%) effluent samples at each plant, respectively. Log reduction ratios of Arcobacter 16S rRNA gene in Plant A and Plant B were 1.7 ± 0.9 (n = 10) and 2.3 ± 1.5 (n = 9), respectively. The ciaB gene was detected by quantitative PCR in eleven (92%) and twelve (100%) of 12 influent samples from Plant A and Plant B, respectively, while the pldA gene was detected in eight (67%) and six (50%) influent samples from Plant A and Plant B, respectively. The prevalence of potentially pathogenic bacteria in WWTP effluent indicated the need for disinfection before discharge into the environment.

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