Performance of the Microplate BacTrace™ ELISA Technique for Detection of Foodborne Salmonella

1990 ◽  
Vol 53 (10) ◽  
pp. 841-845 ◽  
Author(s):  
J.-Y. D'AOUST ◽  
E. DALEY ◽  
A. M. SEWELL
Keyword(s):  
False Positive ◽  
Brilliant Green ◽  
Test System ◽  
Citrobacter Freundii ◽  
High Moisture ◽  
Elisa System ◽  
False Positive Reactions ◽  
Pure Cultures ◽  
Elisa Technique ◽  
Structural Antigen

The developmental BacTrace™ ELISA system which recognizes a common structural antigen (CSA-1) in the cell wall of target microorganisms was tested with pure cultures and naturally contaminated foods. The system readily detected all of the 104 Salmonella test strains but produced 38 (52.1%) false-positive reactions upon examination of 73 nonsalmonellae cultures. Citrobacter freundii, Escherichia coli, and Proteus mirabilis were primarily responsible for erroneous results. Of 119 foods tested, 37 (31.1%) were found to contain Salmonella by a standard cultural procedure. Parallel BacTrace™ testing of the nutrient broth (NB), tetrathionate brilliant green (TBG43), and selenite cystine (SC35) broth cultures arising from standard cultural analyses identified 24 (64.9%), 25 (67.6%), and 31 (83.8%) Salmonella contaminated foods, respectively. Maximum sensitivity of the test system (89.2%) could be attained through combination of ELISA results from both TBG43 and SC35. False-positive reactions were particularly prominent with high moisture foods.

Detection of Salmonella with the BioEnzabead™ Enzyme Immunoassay Technique

1988 ◽  
Vol 51 (7) ◽  
pp. 538-541 ◽  
Author(s):  
J.-Y. D'AOUST ◽  
A. M. SEWELL
Keyword(s):  
Brilliant Green ◽  
Cross Reactivity ◽  
High Rate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Citrobacter Freundii ◽  
High Moisture ◽  
Enrichment Broth ◽  
Immunoassay Technique ◽  
Pure Cultures ◽  
Enzyme Immunoassay Technique

The performance of the double-antibody (MOPC 467 and 6H4) BioEnzabead™ enzyme linked immunosorbent assay was evaluated with pure cultures and naturally contaminated foods. The immunoassay detected all but four (1.6%) of the 250 Salmonella test strains and showed high levels of cross-reactivity (30.7%) among the 75 strains of non salmonellae examined. Citrobacter freundii figured prominently as a source of erroneous results. Although the BioEnzabead™ system identified the 41 foods found to be contaminated by a standard cultural procedure, a high rate of false positive reactions (46%) was nevertheless encountered with high moisture foods. Attempt at method brevity through application of the immunoassay at the preenrichment level was unsuccessful resulting in the detection of only 24 (58.5%) of the 41 contaminated foods. Greater recoveries were obtained with tetrathionate brilliant green enrichment broth incubated at 43°C than at 35°C.

Comparative Efficiency of Brilliant Green, Bismuth Sulfite, Salmonella-Shigella, Hektoen Enteric, and Xylose Lysine Desoxycholate Agars for the Recovery of Salmonella from Foods: Collaborative Study

1981 ◽  
Vol 64 (4) ◽  
pp. 899-928
Author(s):  
Wallace H Andrews ◽  
Paul L Poelma ◽  
Clyde R Wilson ◽  
◽  
C Abeyta ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
False Positive ◽  
Relative Efficiency ◽  
Brilliant Green ◽  
Collaborative Study ◽  
False Negative ◽  
Reaction Rates ◽  
Negative Reaction ◽  
Comparative Efficiency ◽  
False Positive Reactions

Abstract The relative efficiency of brilliant green (BG), bismuth sulfite (BS), Salmonella-Shigella (SS), xylose lysine desoxycholate (XLD), and Hektoen enteric (HE) agars for the recovery of Salmonella from 5 foods was collaboratively studied in 11 laboratories. The analytical efficiency of various paired combinations of the 5 agars was statistically compared according to 3 parameters: (1) productivity or recovery of Salmonella, (2) rate of enumeration of cultures that were false positive for Salmonella, and (3) rate of enumeration of false-negative reactions. In descending order of productivity, the sequential rankingwasBS, XLD, HE, BG, and SS agars. In ascending order, the rates of false-positive reactions based on a statistical analysis of paired agar combinations was HE, BS, BG and XLD (tie), and SS agars. Analogously, in ascending order, the sequence of false-negative reaction rates was BS, XLD, HE, BG, and SS agars. The combination of BS, XLD, and HE agars is more efficient for recovery of Salmonella from foods than is the present official combination of BG, BS, and SS agars. The revision of official final action method 46.054 to replace the combination of BG, BS, and SS agars with a combination of BS, XLD, and HE agars has been adopted official first action.

scholarly journals Patch testing with aluminium Finn Chambers could give false positive reactions in patients with contact allergy to aluminium

2021 ◽  
Author(s):  
Lisbeth Rosholm Comstedt ◽  
Jakob Dahlin ◽  
Magnus Bruze ◽  
Youlanda Hedberg ◽  
Mihály Matura ◽  
...  
Keyword(s):  
Patch Testing ◽  
False Positive ◽  
Contact Allergy ◽  
False Positive Reactions

scholarly journals False Positive Reactions in Treponemal Tests

1963 ◽  
Vol 39 (1) ◽  
pp. 30-32
Author(s):  
A. J. Julian ◽  
J. Portnoy ◽  
T. L. H. N. Bossak
Keyword(s):  
False Positive ◽  
False Positive Reactions

scholarly journals False-Positive Reactions Against HLA Class II Molecules Detected in Luminex Single-Antigen Bead Assays

2014 ◽  
Vol 34 (5) ◽  
pp. 408-410 ◽  
Author(s):  
Ji Won In ◽  
Eun Youn Rho ◽  
Sue Shin ◽  
Kyoung Un Park ◽  
Eun Young Song
Keyword(s):  
False Positive ◽  
Class Ii ◽  
Hla Class Ii ◽  
False Positive Reactions ◽  
Single Antigen ◽  
Hla Class Ii Molecules

scholarly journals A comparison of ten USEPA approved total coliform/E. coli tests

2007 ◽  
Vol 5 (2) ◽  
pp. 267-282 ◽  
Author(s):  
Jeremy Olstadt ◽  
James Jay Schauer ◽  
Jon Standridge ◽  
Sharon Kluender
Keyword(s):  
Environmental Protection Agency ◽  
False Positive ◽  
The United States ◽  
Total Coliform ◽  
Test System ◽  
E Coli ◽  
Test Systems ◽  
High Concentrations ◽  
Different Strains ◽  
Positive Results

Since 2002, the United States Environmental Protection Agency (USEPA) has approved ten enzyme-based total coliform and E. coli detection tests for examination of drinking water. These tests include: Colilert®, Colilert-18®, Colisure®, m-Coli Blue 24®, Readycult® Coliforms 100, Chromocult®, Coliscan®, E*Colite®, Colitag™ and MI Agar. The utility of the enzyme based test systems is based on both the ability of the test to detect the target organisms at low levels and the ability of the test system to suppress the growth of non-target organisms that might result in false positive results. Differences in the ability of some of these methods to detect total coliform and E. coli, as well as suppress Aeromonas spp., a common cause of “false positive” results, have been observed. As a result, this study was undertaken to elucidate the strengths and weaknesses of each method. Water samples were collected from three geographically and chemically diverse groundwaters in Wisconsin. One-hundred milliliter aliquots were individually spiked with both low concentrations (one to ten organisms) and high concentrations (fifty to one-hundred) of each of five different total coliform organisms (Serratia, Citrobacter, Enterobacter, E. coli, & Klebsiella). These spiked samples were used to test the capability of ten enzyme-based test systems to both detect and enumerate the spiked organisms. In addition, 100 ml samples were independently spiked with two different strains of Aeromonas spp. at six different levels, to assess the ability of each enzyme-based test to suppress Aeromonas spp. Analysis of the data indicated that wide variability exists among USEPA approved tests to detect and quantify total coliforms, as well as suppress Aeromonas spp.

Marine Biosurfactants, III. Toxicity Testing with Marine Microorganisms and Comparison with Synthetic Surfactants

1991 ◽  
Vol 46 (3-4) ◽  
pp. 210-216 ◽  
Author(s):  
Knut Poremba ◽  
Wilfried Gunkel ◽  
Siegmund Lang ◽  
Fritz Wagner
Keyword(s):  
Marine Bacterium ◽  
Sea Water ◽  
Toxicity Testing ◽  
Test System ◽  
Marine Microorganisms ◽  
Oil Dispersants ◽  
Test Organisms ◽  
Pure Cultures ◽  
Microtox Test ◽  
Synthetic Surfactants

Eight synthetic and nine biogenetic surfactants were tested on their toxicity. Because of their possible application as oil dispersants against oil slicks on sea. the test organisms used were marine microorganisms (mixed and pure cultures of bacteria, microalgae, and protozoa). Bacterial growth was hardly effected or stimulated, whilst that of algae and flagellates was reduced. All substances tested were biodegradaded in sea water. The bioluminescence of Photobacter phosphoreum (Microtox test) was the most sensitive test system used. A ranking shows that most biogenetic surfactants were less toxic than synthetic surfactants. No toxicity could be detected with the glucose-lipid GL. produced by the marine bacterium Alcaligenes sp. MM 1.

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