Herpes Simplex Virus Type 1 Applied Experimentally to Gloves Used for Food Preparation

Droplets of saliva containing herpes simplex virus type 1 were placed on latex disposable gloves. The temperature at the surface of the gloved hand was 34°C. There was no loss of infectious virus before 15 min. Between 15 and 30 min there was a 2-log-cycle drop in titer, and infectious virus could still be recovered after 1 h, the longest period tested. The drop in titer was due to drying of the saliva, which occurred at approximately 21 min. Infectious virus was transferred by touch to lettuce and ham at 0 min when the virus-containing droplets were in a liquid condition, and after 30 and 60 min when the droplets were dry.

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Survival of Herpes Simplex Virus Type 1 on Some Common Foods Routinely Touched before Consumption

Journal of Food Protection ◽

10.4315/0362-028x-60.10.1259 ◽

1997 ◽

Vol 60 (10) ◽

pp. 1259-1261 ◽

Cited By ~ 3

Author(s):

D. BARDELL

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

Virus Type ◽

Herpes Simplex Virus Type ◽

Infectious Virus ◽

Virus Infectivity ◽

Refrigerator Temperature ◽

Simplex Virus ◽

Infectivity Titer

Droplets of saliva containing herpes simplex virus type 1 were placed on the skin of tomatoes and the upper surface of lettuce leaves. There was no loss of virus infectivity titer at refrigerator temperature (2°C) at any time examined up to 1 h, the longest period tested. At room temperature (22 to 24°C) there was a 2-log drop in titer between 30 and 60 min, but some infectious virus was still present at 1 h. The virus-containing saliva remained in a liquid state at 2°C. At 22 to 24°C the droplets became dry at approximately 50 min. Implications of the findings are discussed.

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Herpes Simplex Virus Type 1 Cytoplasmic Envelopment Requires Functional Vps4

Journal of Virology ◽

10.1128/jvi.00222-07 ◽

2007 ◽

Vol 81 (14) ◽

pp. 7380-7387 ◽

Cited By ~ 97

Author(s):

Colin M. Crump ◽

Catherine Yates ◽

Tony Minson

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

Virus Type ◽

Herpes Simplex Virus Type ◽

Infectious Virus ◽

Virus Production ◽

Dominant Negative ◽

Simplex Virus ◽

Multivesicular Endosomes

ABSTRACT The assembly and egress of herpesviruses are complex processes that require the budding of viral nucleocapsids into the lumen of cytoplasmic compartments to form mature infectious virus. This envelopment stage shares many characteristics with the formation of luminal vesicles in multivesicular endosomes. Through expression of dominant-negative Vps4, an enzyme that is essential for the formation of luminal vesicles in multivesicular endosomes, we now show that Vps4 function is required for the cytoplasmic envelopment of herpes simplex virus type 1. This is the first example of a large enveloped DNA virus engaging the multivesicular endosome sorting machinery to enable infectious virus production.

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The Influence of Cyclosporin Immunosuppression on the Efficacy of Famciclovir or Valaciclovir Chemotherapy Studied in a Murine Herpes Simplex Virus Type 1 Infection Model

Antiviral Chemistry and Chemotherapy ◽

10.1177/095632029700800404 ◽

1997 ◽

Vol 8 (4) ◽

pp. 317-326 ◽

Cited By ~ 5

Author(s):

AM Thackray ◽

HJ Field

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

Virus Type ◽

Herpes Simplex Virus Type ◽

Infectious Virus ◽

Clinical Signs ◽

Infection Model ◽

Post Inoculation ◽

Simplex Virus

Mice with or without immunosuppression by cyclosporin (Cy) were inoculated with herpes simplex virus type 1 in the ear pinna. Without immunosuppression, 20% of the mice died; clinical signs resolved in survivors and infectious virus was cleared by 7 to 10 days post-inoculation (p.i.). With Cy, mortality was 50%, clinical signs increased and infectious virus persisted. Mice were treated with either valaciclovir (VACV) or famciclovir (FCV) from days 1–5 or 5–10 p.i. and both compounds moderated the disease, but only FCV led to rapid restoration of body weight and complete protection from mortality. Resolution of clinical signs was more marked with immunosuppression. On cessation of VACV therapy, infectious virus recurred on individual days. Without immunosuppression, recurrence was detected in neural tissues only, but with Cy, infectious virus also recurred in skin. No recurrences of infectious virus were observed in any FCV-treated mice.

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