Determination of Fumonisins B1 and B2 in Herbal Tea and Medicinal Plants in Turkey by High-Performance Liquid Chromatography

2004 ◽  
Vol 67 (8) ◽  
pp. 1782-1786 ◽  
Author(s):  
GÜLDEN Z. OMURTAG ◽  
DUYGU YAZICIOĞILU
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Medicinal Plants ◽  
High Performance ◽  
Fumonisin B1 ◽  
Black Tea ◽  
Herbal Tea ◽  
Two Samples ◽  
Fumonisin B2

The purpose of this study was to measure the potential levels of fumonisin B1 (FB1) and fumonisin B2 (FB2) contamination in several herbal teas and medicinal plants that are consumed regularly in Turkey. FB1 and FB2 were detected using high-performance liquid chromatography with fluorescence detection after derivatization with o-phthaldialdehyde. A total of 115 commercially available herbal tea and medicinal plant samples were analyzed. The recoveries in black tea were 86.9 ± 8.42% for FB1 and 102 ± 6.80% for FB2 spiked with 1 μg/g of each analyte. Similarly, the mean recovery results in lime (linden) for FB1 and FB2 were 85.2 ± 9.76% and 78.6 ± 5.67%, respectively. The minimum detectable amounts for the o-phthaldialdehyde derivatives of FB1 and FB2 were 0.025 μg/g (1 ng injected) and 0.125 μg/g (5 ng), respectively. FB1 was detected in two samples (0.160 and 1.487 μg/g), and FB2 was detected in none of the samples.

Development of reverse phase-high performance liquid chromatography (RP-HPLC) method for determination of selected antihypertensive active flavonoids (rutin, myricetin, quercetin, and kaempferol) in medicinal plants found in Botswana

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Katso Binang ◽  
David T. Takuwa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Medicinal Plants ◽  
High Performance ◽  
Zingiber Officinale ◽  
Hplc Method ◽  
Reverse Phase ◽  
Rp Hplc ◽  
Lippia Javanica

Abstract The aim of the study was to develop a rapid, efficient, and cheap chromatographic method for determining four selected antihypertensive active flavonoid compounds in medicinal plants in Botswana. The determination of rutin, quercetin, and kaempferol in selected medicinal plants was conducted in less than 6 min using the developed reverse phase-high performance liquid chromatography (RP-HPLC) method with a 2.7 µm Ascentis C18 express column (150 × 4.60 mm i.d) at 340, 360, and 368 nm detection wavelengths and mobile phase of methanol and 0.068% of formic acid solution in isocratic elution. Validation results showed good selectivity, linearity (r 2 > 0.99), high percentage recoveries (90.2–104.7%), and precision (% RSD < 2) for n = 3, confirming suitability of the method for determination of the investigated flavonoids in Zingiber officinale (ginger). Application of the developed RP-HPLC method was performed in selected medicinal plants (Lippia javanica ) (mosukujane), Myrothanmus flabellious (galalatshwene), and Elephantorrhiza elephantina (mositsana) used to manage hypertension by herbalists in Botswana. M. flabellious a very commonly used plant for managing hypertension was found to contain highest amounts of rutin and myricetin, whereas nothing was detected for E. elephantina.

scholarly journals Determination of Ferulic Acid in Angelica sinensis by Temperature-Controlled Hydrophobic Ionic Liquids-Based Ultrasound/Heating-Assisted Extraction Coupled with High Performance Liquid Chromatography

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3356
Author(s):  
Hongwei Wu ◽  
Qianqian Huang ◽  
Shujun Chao ◽  
Jie Yu ◽  
Shengrui Xu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Ionic Liquids ◽  
Liquid Chromatography ◽  
Medicinal Plants ◽  
High Performance ◽  
Angelica Sinensis ◽  
Assisted Extraction ◽  
Temperature Controlled ◽  
Hydrophobic Ionic Liquids

Hydrophilic ionic liquids are often used to extract the active ingredients of medicinal plants, while hydrophobic ionic liquids are rarely used to directly extract solid samples. In this paper, a simple, novel and efficient temperature-controlled hydrophobic ionic liquids-based ultrasound/heating-assisted extraction (TC-ILs-UHAE) procedure coupled with high-performance liquid chromatography (HPLC) was developed and applied to the determination of ferulic acid (FA) in Chinese herbal medicine Angelica sinensis. During the extraction procedure, hydrophobic ionic liquids (ILs) were dispersed into water to form cloudy solution (fine droplets) with the aid of ultrasound and heating simultaneous. After extraction, phase separation was easily achieved by centrifuging at 0 °C. Among all ILs used, 1-butyl-3-methylimidazolium bis(trifluoromethanesulphonyl)imide ([C4mim]NTf2) exhibited the highest extraction ability and the possible extraction mechanism was discussed. Additionally, the synergistic effect of heating and ultrasound on the extraction efficiency was investigated. Under the optimized conditions, a good linearity was observed with correlation coefficient (r) of 0.9995. The limit of detection of FA (LOD, S/N = 3) was 9.6 µg/L and the spiked recoveries of FA for real samples were in the range of 91.67 to 102.00% with relative standard deviation (RSD) lower than 3.87%. Compared with the traditional extraction methods, the proposed method gave the highest yield of FA and had the shortest extraction time. Therefore, this method is a potential simple, green and highly efficient technique and expected to be applied to the extraction of other bioactive ingredients in medicinal plants.

Determination of Fumonisin B1 and B2 in Corn and Corn-Based Products in Turkey by High-Performance Liquid Chromatography

2001 ◽  
Vol 64 (7) ◽  
pp. 1072-1075 ◽  
Author(s):  
GÜLDEN Z. OMURTAG
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
High Performance ◽  
Fumonisin B1 ◽  
Turkish Population ◽  
Detectable Amount ◽  
Derivatives Of ◽  
Minimum Detectable Amount

The purpose of this study was to investigate fumonisin B1 (FB1)- and B2 (FB2)-contaminated corn and corn-based products consumed especially by the Turkish population. FB1 and FB2 were detected using high-performance liquid chromatography with fluorescence detection. The total number of commercially available corn and corn-based product samples analyzed in this research was 82. The recoveries were found to be 94.4 ± 4.62% and 86.5 ± 4.86% for cornmeal spiked with known amounts of FB1 and FB2 (1 ppm), respectively. The minimum detectable amount for the o-phthaldialdehyde derivatives of FB1 and FB2 were 1 ng and 5 ng, respectively. Detected levels of FB1 were between 0.25 ppm and 2.66 ppm in 25.6% of the samples, and detected level of FB2 in a single cornmeal sample was 0.55 ppm.

Fumonisin Production by Fusarium Species Isolated from Cereals and Feeds in Spain

1999 ◽  
Vol 62 (7) ◽  
pp. 811-813 ◽  
Author(s):  
G. CASTELLÁ ◽  
M. R. BRAGULAT ◽  
F. J. CABAÑES
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Fusarium Species ◽  
Fumonisin B1 ◽  
Fluorescence Method ◽  
Fumonisin B2 ◽  
Corn Kernels

The ability of Fusarium species to produce fumonisins was studied with 145 isolates of the following species: F. moniliforme (119 isolates), F. subglutinans (12 isolates), F. proliferatum (9 isolates), F. avenaceum (1 isolate), F. oxysporum (1 isolate), and F. semitectum (3 isolates). All isolates were cultured on autoclaved corn kernels. The production of fumonisins was determined by a high-performance liquid chromatography-o-phthaldialdehyde-fluorescence method. Fumonisin production was restricted to isolates of F. moniliforme (94.1%) and F. proliferatum (100%), in the section Liseola, including all strains isolated from wheat, barley, peas, and soybean. One strain of F. proliferatum isolated from maize produced 30,949 μg/g of fumonisin B1 and 16,966 μg/g of fumonisin B2.

scholarly journals Simultaneous determination of artificial sweeteners in possible counterfeited wines, using high performance liquid chromatography with DAD detection

2012 ◽  
Vol 23 (1) ◽  
pp. 77-81
Author(s):  
Elisabeta-Irina Geana ◽  
Andreea-Maria Iordache ◽  
Roxana Elena Ionete
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Quantitative Methods ◽  
Wine Industry ◽  
Artificial Sweeteners ◽  
Official Method ◽  
Sweet Wines ◽  
Two Samples

Abstract Increased use of food, beverages or drugs containing synthetic sweeteners presents a real danger to health, which is why the EU Member States had to establish a system of regular surveys to monitor sweetener consumption. In case of wine industry, according to law no. 244/2002, Romania prohibits the addition of synthetic sweeteners in wine in order to obtain sweet wine. The official method for detection of adulterated sweet wines with synthetic sweeteners is TLC-Thin Layer Chromatography. However, quantitative methods of analysis are needed to measure levels of sweeteners in different food matrices and high performance liquid chromatography has proved to be a powerful tool for quantitative analysis of compounds at traces levels. In this paper, a high performance liquid chromatographic (HPLC) method for the simultaneous separation and determination of three of the most popular artificial sweeteners (acesulfame potassium, saccharine and aspartame) in a single injection was developed. The described method is rapid, accurate and highly sensitive. Detection limit were 4 mg/L for acesulfame K, 1 mg/L for saccharine and 9 mg/L for aspartame respectively. The precision of the method was about 2% and recovery ranged between 92.6% and 103.3%. There were analyzed commercial wine samples, in order to detect possible counterfeits-sweet and medium sweet wines. Therefore, of the 20 analyzed wine samples, only two samples consisting in wine sweet table, were counterfeited by adding saccharin.

Determination of Fumonisin B1 in Corn by High Performance Liquid Chromatography With Fluorescence Detection

1993 ◽  
Vol 26 (8) ◽  
pp. 1751-1770 ◽  
Author(s):  
George M. Ware ◽  
Octave Francis ◽  
Shia S. Kuan Pesi Umrigar ◽  
Allen Carman ◽  
Lawrence Carter ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
High Performance ◽  
Fumonisin B1
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