Effects of Lactobacillus plantarum, Pediococcus acidilactici, and Pediococcus pentosaceus on the Growth of Listeria monocytogenes and Salmonella on Alfalfa Sprouts

2019 ◽  
Vol 82 (3) ◽  
pp. 522-527 ◽  
Author(s):  
FRANCA ROSSI ◽  
AMANDA LATHROP
Keyword(s):  
Listeria Monocytogenes ◽  
Lactobacillus Plantarum ◽  
High Temperatures ◽  
Pediococcus Acidilactici ◽  
Low Light ◽  
Pediococcus Pentosaceus ◽  
Initial Concentration ◽  
Seedling Length ◽  
Survival And Growth ◽  
Alfalfa Sprouts

ABSTRACT The germination conditions of sprouted vegetables consisting of relatively high temperatures and humidity, low light, and abundance of nutrients are ideal for pathogen survival and growth. The continual occurrence of outbreaks and recalls associated with sprout vegetables indicate additional measures are needed to improve product safety. The objective of this study was to evaluate the efficacy of a mixture of Lactobacillus plantarum, Pediococcus acidilactici, and Pediococcus pentosaceus (LPP) against Listeria monocytogenes and Salmonella on alfalfa sprouts during 5 days of sprouting at 20°C and its influence on sprout quality. Alfalfa seeds were inoculated with L. monocytogenes or Salmonella (each at 1 and 3 log CFU/g) and LPP (7 log CFU/g). Populations of LPP were maintained at 7.5 to 8.0 log CFU/g throughout sprouting. LPP had a significant effect on the growth of L. monocytogenes and Salmonella (P < 0.05). After 5 days of sprouting, populations of L. monocytogenes at an initial concentration of 1 and 3 log CFU/g of seeds treated with LPP were approximately 4.5 and 1.0 log CFU/g less than the untreated seeds, respectively. Populations of Salmonella at an initial concentration of 1 and 3 log CFU/g were 1.0 log CFU/g less than the control. LPP did not compromise the yield, seedling length, or pH of the sprouts.

Growth and Metabolite Profile of Pediococcus pentosaceus and Lactobacillus plantarum in Different Juice

2015 ◽  
Vol 1 (1) ◽  
pp. 1 ◽  
Author(s):  
Soundarrajan Ilavenil ◽  
Srisesharam Srigopalram ◽  
Hyung Soo Park ◽  
Ki Choon Choi
Keyword(s):  
Lactobacillus Plantarum ◽  
Metabolite Profile ◽  
Pediococcus Pentosaceus

Growth of Listeria monocytogenes in milk and its control by pediocin 5 produced by Pediococcus acidilactici UL5

1994 ◽  
Vol 4 (5) ◽  
pp. 429-443 ◽  
Author(s):  
Jun Huang ◽  
Christophe Lacroix ◽  
Hocine Daba ◽  
Ronald E. Simard
Keyword(s):  
Listeria Monocytogenes ◽  
Pediococcus Acidilactici

Performance of stress resistant variants of Listeria monocytogenes in mixed species biofilms with Lactobacillus plantarum

2015 ◽  
Vol 213 ◽  
pp. 24-30 ◽  
Author(s):  
Karin I. Metselaar ◽  
Paula Saá Ibusquiza ◽  
Angela R. Ortiz Camargo ◽  
Myriam Krieg ◽  
Marcel H. Zwietering ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Lactobacillus Plantarum ◽  
Mixed Species

Improving the quality of Suancai by inoculating with Lactobacillus plantarum and Pediococcus pentosaceus

2021 ◽  
pp. 110581
Author(s):  
Ge Song ◽  
Zhen He ◽  
Xinyi Wang ◽  
Mingwei Zhao ◽  
Xinying Cao ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Pediococcus Pentosaceus

scholarly journals Perfil fermentativo, estabilidade aeróbia e valor nutritivo de silagens de capim-marandu ensilado com aditivos

2008 ◽  
Vol 37 (10) ◽  
pp. 1728-1736 ◽  
Author(s):  
Thiago Fernandes Bernardes ◽  
Ricardo Andrade Reis ◽  
Rafael Camargo do Amaral ◽  
Gustavo Rezende Siqueira ◽  
Anna Paula de Toledo Piza Roth ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Pediococcus Acidilactici ◽  
Lactobacillus Buchneri

Com o objetivo de avaliar as perdas em silagem de capim-marandu produzidas com aditivos foram desenvolvidos dois experimentos. No experimento 1, objetivou-se conhecer o perfil de fermentação e a estabilidade aeróbia de quatro silagens: 1) forragem não tratada (Controle); 2) tratada com Lactobacillus plantarum e Propionibacterium; 3) tratada com Lactobacillus buchneri; e 4) tratada com 0,1% de benzoato de sódio. No experimento 2, foram utilizados nove novilhos castrados Nelore (PC de 350 ± 38,9 kg), alocados em três quadrados latinos 3 x 3 para avaliação do consumo e da digestibilidade das rações contendo 85,4% das seguintes silagens de capim-marandu: 1) controle; 2) controle com L. plantarum, Pediococcus acidilactici + enzimas fibrolíticas; e 3) tratamento 2 + L. buchneri. No experimento 1, as silagens apresentaram baixas recuperações de MS durante a fermentação (média de 86%) e os coeficientes de digestibilidade in vitro da matéria seca reduziram de 65,5% (momento da ensilagem) para 50,0% no 60º dia após o fechamento dos silos. No experimento 2, o valor médio de consumo das rações foi de 5,7 kg MS/dia (1,6% PC) e a digestibilidade de 51,6% e não diferiram entre as rações. As silagens apresentaram perdas acentuadas na fase fermentativa e o uso de aditivos não alterou essas perdas. A inoculação com bactérias não influenciou o consumo ou a digestibilidade das rações.

scholarly journals Enhanced Bacteriocin Production by Pediococcus pentosaceus 147 in Co-culture With Lactobacillus plantarum LE27 on Cheese Whey Broth

2018 ◽  
Vol 9 ◽  
Author(s):  
Carolina Gutiérrez-Cortés ◽  
Héctor Suarez ◽  
Gustavo Buitrago ◽  
Luis Augusto Nero ◽  
Svetoslav Dimitrov Todorov
Keyword(s):  
Lactobacillus Plantarum ◽  
Cheese Whey ◽  
Bacteriocin Production ◽  
Pediococcus Pentosaceus

Bacteriocin Inhibition of Clostridium botulinum Spores by Lactic Acid Bacteria

1991 ◽  
Vol 54 (5) ◽  
pp. 349-353 ◽  
Author(s):  
AMECHI OKEREKE ◽  
THOMAS J. MONTVILLE
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Acidophilus ◽  
Clostridium Botulinum ◽  
Lytic Phage ◽  
Pediococcus Pentosaceus ◽  
Inhibitory Cell ◽  
Simultaneous Testing ◽  
Inhibition Zones

Twenty-three strains of lactic acid bacteria were tested by deferred antagonism methods for bacteriocin-like activity against types A and B spores from 11 proteolytic and nonproteolytic Clostridium botulinum strains. Pediococcus pentosaceus ATCC 43200, Pediococcus pentosaceus ATCC 43201, Lactococcus lactis subsp. lactis ATCC 11454, Lactobacillus acidophilus N2, Lactobacillus plantarum Lb75, Lactobacillus plantarum Lb592, and Lactobacillus plantarum BN exhibited bacteriocin-like inhibition of all C. botulinum strains tested. By excluding inhibition due to hydrogen peroxide, acid, and lytic phage and confirming their proteinaceous nature, the inhibitors were confirmed as bacteriocins. The minimum inhibitory cell concentrations (MICC) required to produce 1 mm radius inhibition zones were determined by direct antagonism testing. Only strains 43200, 43201, 11454, and N2 were inhibitory when cultured simultaneously with the botulinal spores. The MICCs of strains antagonistic to C. botulinum spores by simultaneous testing ranged between 1.6 × 105and 4.7 × 107CFU/ml. Based on the MICCs, P. pentosaceus 43200 was most inhibitory to C. botulinum.

scholarly journals Estudio del potencial probiótico de lactobacilos aislados de fuentes naturales

2003 ◽  
Vol 6 (1) ◽  
pp. 30-35
Author(s):  
Karin L. Zamudio. ◽  
Amparo I. Zavaleta.
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Lactobacillus Plantarum

El uso de los lactobacilos como probióticos se ha incrementado debido a sus propiedades benéficas para la salud animal y humana. Con el objetivo de identificar y obtener cepas nativas de lactobacilos para ser utilizadas como probióticos, se seleccionaron 9 lactobacilos aislados de diferentes fuentes naturales. Las especies de Lactobacillus fueron identificadas por el perfil de fermentación de carbohidratos y por la Reacción en Cadena de la Polimerasa utilizando cebadores específicos diseñados a partir de los espaciadores intergénicos de los genes ribosómicos 16S-23S. Los lactobacilos seleccionados pertenecen a las especies: Lactobacillus plantarum, Lb. acidophilus, Lb. casei y Lb. fermentum correspondiendo en número de cepas por especie 3, 3, 2 y 1 respectivamente. El potencial probiótico de estos microorganismos se determinó por su estabilidad frente a pH básico, su tolerancia a la bilis y a su actividad antimicrobiana contra los patógenos entéricos Listería monocytogenes y Escherichia coli. Se encontró que Lb. acidophilus 4P-1 presenta las mejores características probióticas por resistir más de 24 horas a pH 3.5 e inhibir a los patógenos ensayados.

Survival and Growth of Listeria monocytogenes on Fresh-Cut “Athena” and “Rocky Ford” Cantaloupes During Storage at 4°C and 10°C

2016 ◽  
Vol 13 (11) ◽  
pp. 587-591 ◽  
Author(s):  
Esmond Nyarko ◽  
Kalmia E. Kniel ◽  
Russell Reynnells ◽  
Cheryl East ◽  
Eric T. Handy ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Survival And Growth ◽  
Fresh Cut

Inhibition of Listeria monocytogenes by Lactobacillus plantarum is abolished by food intake, in an in vitro simulation of the pig colon

1998 ◽  
Vol 1998 ◽  
pp. 164-164
Author(s):  
K. Hillman ◽  
R. Khaddour ◽  
D. R. Fenlon
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Food Intake ◽  
Lactobacillus Plantarum ◽  
Enteric Pathogen ◽  
Intestinal Health ◽  
Microbial Cultures ◽  
In Vitro Simulation ◽  
Physiological Differences

Probiotics are live microbial cultures fed to animals with the intention of promoting intestinal health. However, one of the problems with these preparations is the variability in their effectiveness, reasons for which include genetic and physiological differences between herds of animals or between individuals, and the type of feed presented to the animals. This study has examined the effects of different food types on the efficacy of a strain of Lactobacillus plantarum as an inhibitor of Listeria monocytogenes. The strain of L. plantarum used (PF31) has been previously shown to inhibit the growth of the porcine enteric pathogen Escherichia coli O 149:K88:K91 in coculture (Hillman and Fox, 1994). In a subsequent series of tests, L. plantarum PF31 was also shown to inhibit the growth of List, monocytogenes in coculture.

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