scholarly journals EVALUATION OF THE PRESENCE OF COLOSTRAL ANTIBODIES IN PIGLETS OF DIFFERENT AGE ORIGINATING FROM SOWS VACCINATED WITH CHINA CLASSICAL SWINE FEVER VIRUS

2009 ◽  
Vol 2 (1) ◽  
pp. 21-27
Author(s):  
Jasna Prodanov Radulović ◽  
Radoslav Došen ◽  
Dušan Orlić ◽  
Marko Maljković ◽  
Miroslav Valčić ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Life Time ◽  
Elisa Test ◽  
Fever Virus ◽  
Maternal Antibodies ◽  
Future Research ◽  
Precise Control ◽  
Immunoenzyme Method ◽  
Modified Live Vaccine

Since the placenta is multilayered, there is no transfer of maternal antibodies into the pig fetal circulation, so passive immunity is completely acquired by post-farrowing colostral intake. When speaking about investigation of maternal immunity, the problem of lasting colostral protection of piglets deriving from sows that were repeatedly vaccinated with modified live vaccine (China strain - C strain) is stressed. The objective of this investigation was to detect the presence of maternal antibodies against classical swine fever virus (CSFV) in piglets of different age deriving from sows that were repeatedly vaccinated with China strain CSFV. The examination was carried out on 35 piglets of different age deriving from sows that were repeatedly vaccinated with C-virus strain of CSF by applying immunoenzyme method (ELISA test). On the bases of obtained serological results, it may be concluded that in farm conditions there was a certain number of piglets that did not suck sufficient quantity of colostrum in early post-farrowing period and the problem may be analyzed from the aspect of half-life time of maternal antibodies against CSF. Certainly, future research should include laboratory methods that can provide more precise control of changed values of antibody titre against CSFV, both in sows and in piglets of different age with the aim to view the dynamics of persistence specific antibodies against CSFV in vaccinated pig population.

scholarly journals Genotyping and Molecular Characterization of Classical Swine Fever Virus Isolated in China during 2016–2018

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 664
Author(s):  
Madiha Fatima ◽  
Yuzi Luo ◽  
Li Zhang ◽  
Peng-Ying Wang ◽  
Hao Song ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Full Genome ◽  
Genotype 1 ◽  
E2 Gene ◽  
The Past ◽  
Genotype 2 ◽  
Modified Live Vaccine

Classical swine fever (CSF) is a highly contagious disease of swine caused by classical swine fever virus (CSFV). For decades the disease has been controlled in China by a modified live vaccine (C-strain) of genotype 1. The emergent genotype 2 strains have become predominant in China in the past years that are genetically distant from the vaccine strain. Here, we aimed to evaluate the current infectious status of CSF, and for this purpose 24 isolates of CSFV were identified from different areas of China during 2016–2018. Phylogenetic analysis of NS5B, E2 and full genome revealed that the new isolates were clustered into subgenotype 2.1d and 2.1b, while subgenotype 2.1d was predominant. Moreover, E2 and Erns displayed multiple variations in neutralizing epitope regions. Furthermore, the new isolates exhibited capacity to escape C-strain-derived antibody neutralization compared with the Shimen strain (genotype 1). Potential positive selection sites were identified in antigenic regions of E2 and Erns, which are related with antibody binding affinity. Recombination events were predicted in the new isolates with vaccine strains in the E2 gene region. In conclusion, the new isolates showed molecular variations and antigenic alterations, which provide evidence for the emergence of vaccine-escaping mutants and emphasize the need of updated strategies for CSF control.

scholarly journals Seroprevalence against classical swine fever virus in vaccinated pigs in Ho Chi Minh City

2020 ◽  
Vol 19 (03) ◽  
pp. 48-51
Author(s):  
Mai C. Duong
Keyword(s):  
Classical Swine Fever Virus ◽  
Humoral Response ◽  
Classical Swine Fever ◽  
Herd Size ◽  
Elisa Test ◽  
Fever Virus ◽  
Ho Chi Minh City ◽  
Serological Response ◽  
Post Vaccination ◽  
Test Kit

The aim of this study was to survey the serological response to classical swine fever disease in vaccinated pigs in Cu Chi, Ho Chi Minh City. By using the PrioCHECK® CSFV Ab 2.0 ELISA test kit to detect antibodies against CSF in 410 vaccinated pigs and IDEXX CSFV Ag Serum Plus Test to detect the Erns protein of the CSFV in pigs without antibodies against CSFV. Results showed that the overall seroprevalence observed in vaccinated pigs in other Farms varied from 70% - 100% (P < 0.05), but in Farm 5, no pigs produced a positive humoral response against CSFV were found. The highest seroprevalence of antibodies against CSFV was found in Farms with a herd size of ≥ 1000 - < 6000 animals (91.26%) and the lowest was a Farms with less than 1000 animals (51.81%). The highest ratio of positive pigs for antibodies against CSFV belonging to Group of > 40 - ≤ 60 days post-vaccination was 98.36%. The lowest rate was found in Group of 30 - ≤ 40 days postvaccination (51.96%). That grower pigs had the highest proportion of positive pigs for antibodies against CSFV accounting for 81.40%; next, the proportion of positive sows was 73.24%. Significant differences in the seroprevalence observed in vaccinated pigs across herd size, days post-vaccination, type of pigs (P < 0.05). In this study, no pig was found to contain CSFV antigen.

ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells

2021 ◽  
Vol 255 ◽  
pp. 109034
Author(s):  
Liang Zhang ◽  
Mingxing Jin ◽  
Mengzhao Song ◽  
Shanchuan Liu ◽  
Tao Wang ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Ns5a Protein

Genome Variability of Classical Swine Fever Virus during the 2018–2020 Epidemic in Japan

2021 ◽  
pp. 109128
Author(s):  
Tatsuya Nishi ◽  
Katsuhiko Fukai ◽  
Tomoko Kato ◽  
Kotaro Sawai ◽  
Takehisa Yamamoto
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Genome Variability

scholarly journals A Novel E2 Glycoprotein Subunit Marker Vaccine Produced in Plant Is Able to Prevent Classical Swine Fever Virus Vertical Transmission after Double Vaccination

Vaccines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 418
Author(s):  
Youngmin Park ◽  
Yeonsu Oh ◽  
Miaomiao Wang ◽  
Llilianne Ganges ◽  
José Alejandro Bohórquez ◽  
...  
Keyword(s):  
Vertical Transmission ◽  
Classical Swine Fever Virus ◽  
Neutralizing Antibodies ◽  
Subunit Vaccine ◽  
Vaccine Dose ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Tissue Samples ◽  
Marker Vaccine ◽  
E2 Glycoprotein

The efficacy of a novel subunit vaccine candidate, based in the CSFV E2 glycoprotein produced in plants to prevent classical swine fever virus (CSFV) vertical transmission, was evaluated. A Nicotiana benthamiana tissue culture system was used to obtain a stable production of the E2-glycoprotein fused to the porcine Fc region of IgG. Ten pregnant sows were divided into three groups: Groups 1 and 2 (four sows each) were vaccinated with either 100 μg/dose or 300 μg/dose of the subunit vaccine at 64 days of pregnancy. Group 3 (two sows) was injected with PBS. Groups 1 and 2 were boosted with the same vaccine dose. At 10 days post second vaccination, the sows in Groups 2 and 3 were challenged with a highly virulent CSFV strain. The vaccinated sows remained clinically healthy and seroconverted rapidly, showing efficient neutralizing antibodies. The fetuses from vaccinated sows did not show gross lesions, and all analyzed tissue samples tested negative for CSFV replication. However, fetuses of non-vaccinated sows had high CSFV replication in tested tissue samples. The results suggested that in vaccinated sows, the plant produced E2 marker vaccine induced the protective immunogenicity at challenge, leading to protection from vertical transmission to fetuses.

scholarly journals Classical swine fever virus employs the PERK- and IRE1-dependent autophagy for viral replication in cultured cells.

Virulence ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 130-149
Author(s):  
Erpeng Zhu ◽  
Huawei Wu ◽  
Wenxian Chen ◽  
Yuwei Qin ◽  
Jiameng Liu ◽  
...  
Keyword(s):  
Viral Replication ◽  
Classical Swine Fever Virus ◽  
Cultured Cells ◽  
Classical Swine Fever ◽  
Fever Virus

Genetic heterogeneity of classical swine fever virus in Central Europe

1997 ◽  
Vol 52 (2) ◽  
pp. 195-204 ◽  
Author(s):  
T Stadejek ◽  
Š Vilček ◽  
J.P Lowings ◽  
A Ballagi-Pordány ◽  
D.J Paton ◽  
...  
Keyword(s):  
Central Europe ◽  
Genetic Heterogeneity ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus
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