HEAT STRESS-INDUCED ALTERATIONS IN ANTIOXIDATIVE ENZYMES OF SOME PLANTS OF CUCURBITACEA FAMILY

The effects of high temperatures on melon cvs. Miranda and Poli, watermelon cv. Crimson Tide and zucchini cv. Asma leaves. The leaves obtained from plants were subjected to 35, 40, 45, 50, 55 and 60°C temperatures with gradual increments every 30-minutes. Samples, obtained at each treatment, were analyzed for ascorbic acid content, NADP(H) oxidase, catalase, gluthatione reductase, peroxidases activities and isoperoxidase patterns. The ascorbic acid content slightly increased parallel to temperature increment in zucchini but did not change in watermelon and in both melon cultivars. Melon cv. Poli exhibited comparatively less oxidative damage than cv. Miranda with a lower NAD(P)H oxidase activity. Heat stress induced NAD(P)H activity in watermelon and zucchini comparing to control plants. Results revealed that antioxidative enzyme activities were increased generally up to 50°C then decreased gradually in melon cultivars. Besides cv. Poli generally had higher enzyme activities than cv. Miranda. The activity of catalaes become prominent in watermelon while the activity of ascorbate peroxidase become prominent in zucchini. Acidic isoperoxidase bands with different relative mobility values were found in all species. Besides, basic isoperoxidase band could not be determined in both melon cultivars and watermelon while a basic isoperoxidase band was found in zucchini.

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Effect of Gibberellic Acid and Ethephon on Ascorbic Acid Content And Ascorbic Acid Oxidase Activity of Redhaven Peaches

Canadian Institute of Food Science and Technology Journal ◽

10.1016/s0315-5463(77)73540-0 ◽

1977 ◽

Vol 10 (4) ◽

pp. 233-235 ◽

Cited By ~ 2

Author(s):

M.A. Douglas ◽

J. Vanderstoep ◽

A.T. Paulson

Keyword(s):

Ascorbic Acid ◽

Gibberellic Acid ◽

Acid Content ◽

Oxidase Activity ◽

Ascorbic Acid Content ◽

Acid Oxidase ◽

Ascorbic Acid Oxidase

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Ascorbic acid content and ascorbate oxidase activity in small-leaved linden leaves in urban environments

IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future" ◽

10.26907/978-5-00130-204-9-2019-247 ◽

2019 ◽

Author(s):

P.A. Kuzmin ◽

◽

I.L. Bukharina ◽

A.M. Kuzmina ◽

A.R. Gilyazova ◽

...

Keyword(s):

Ascorbic Acid ◽

Acid Content ◽

Oxidase Activity ◽

Ascorbic Acid Content ◽

Urban Environments ◽

Ascorbate Oxidase

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EFFECT OF ALLYLISOTHIOCYANATE ON ASCORBIC ACID CONTENT OF SHREDDED CABBAGE

HortScience ◽

10.21273/hortsci.27.6.651g ◽

1992 ◽

Vol 27 (6) ◽

pp. 651g-652

Author(s):

Masayasu Nagata ◽

Ichiji Yamashita

Keyword(s):

Ascorbic Acid ◽

Protein Synthesis ◽

Acid Content ◽

Oxidase Activity ◽

De Novo ◽

Biological Activities ◽

Ascorbic Acid Content ◽

Enzymatic Reactions ◽

Ethylene Evolution ◽

Inhibitor Of Protein Synthesis

Allylisothiocyanate (ATTC) is known as a main pungent compound in cabbage, which indicates several biological activities. Our previous studies have suggested that AITC acts as a natural regulator which inhibits browning and ethylene evolution of shredded cabbage. It was found that AITC mainly inhibits de novo protein synthesis and in some case, it partially inhibits enzymatic reactions directly. This time we investigated the changes in ascorbic acid (AsA) content and AsA oxidase activity of shredded cabbage, treated with various amounts of AITC. AsA content of shredded cabbage was initially 16.9 mg/100g fresh wt. After 24 h incubation at 10°C, it was reached to 26.3 mg/100g fresh wt. This increase was suppressed by AITC and also by cycloheximide, well known inhibitor of protein synthesis. It was suggested that the increase in AsA was a result of wounding reaction that took place in shredded cabbage. AsA oxidase activity increased during incubation, which was suppressed by AITC, too. From these results, AITC was thought to be a natural regulator of wounding reactions in cabbage.

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Zinc (Zn) and Iron (Fe) Fertilization for Improving the Antioxidant Enzyme Activity and Biochemical Constituents in Capsicum Hybrids

International Journal of Plant & Soil Science ◽

10.9734/ijpss/2021/v33i2430774 ◽

2021 ◽

pp. 251-261

Author(s):

Prakash Ranjan Behera ◽

T. Chitdeshwari ◽

P. Malarvizhi ◽

U. Sivakumar ◽

P. Irene Vethamoni

Keyword(s):

Ascorbic Acid ◽

Antioxidant Enzyme ◽

Enzyme Activities ◽

Acid Content ◽

Ascorbic Acid Content ◽

Soluble Solids ◽

Antioxidant Enzyme Activities ◽

Fresh Weight ◽

Total Soluble Solids ◽

Biochemical Constituents

Micronutrients, particularly Iron (Fe) and Zinc (Zn), play a vital role in the growth and development of plants due to their catalytic effect on many metabolic processes. However, the biochemical responses to the applied micronutrients vary with cultivars and their species. A screening experiment was conducted during 2020 to know the antioxidants enzyme activities and biochemical constituents in response to iron and zinc fertilization by six capsicum hybrids grown in grow bags under shade net conditions. The experiment consists of three treatments viz., Control (No Fe & Zn), 50 kg FeSO4 and 37.5 kg ZnSO4 ha-1 as a basal soil application with six capsicum hybrids viz., Indra, Priyanka, Inspiration, Massilia, Bachata, and Local green. Leaf samples of the capsicum hybrids were collected at Fruiting stage and analysed for antioxidant enzyme activities. The fruit samples were used for quantifying the biochemical constituents. The results revealed that, application of ferrous sulphate (FeSO4) and zinc sulphate (ZnSO4) to capsicum hybrids increased the biochemical constituents in fruits and the antioxidant enzyme activities in leaves. Out of the six hybrids tested, Indra possessed higher ascorbic acid content (9.20 mg 100 g-1 fresh weight), acidity (6.0), and total soluble solids (6.10 Brix) in the fruits, which was followed by Inspiration and Bachata. The superoxide dismutase (6.70 unit’s mg-1 protein) and peroxidase (6.90 unit’s g-1 fresh weight) activities were also higher with the same genotypes. The biochemical constituents and antioxidant enzyme response to Zn addition was better than Fe. There was 13.2, 10.9 and 9.5 per cent increase in titratable acidity, total soluble solids, ascorbic acid content in the fruits of Indra due to ZnSO4 application.The Principal Component Analysis (PCA) and hierarchical clustering revealed that Indra is highly responsive to Zn and Fe fertilization, while the local green showed very less response. The rest of the genotypes such as Inspiration, Bachata, Massilia, and Priyanka, are medium responsive for Zn and Fe fertilization.

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