scholarly journals In vitro Antimicrobial Activity of Ethanolic Leaf Extracts of Hibiscus Asper Hook. F. and Hibiscus Sabdariffa L. on some Pathogenic Bacteria

2021 ◽  
Vol 2 (3) ◽  
pp. 1-12
Author(s):  
Joseph Olowo Arogbodo ◽  
Oyetayo Bolanle Faluyi ◽  
Festus Omotere Igbe
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Hibiscus Sabdariffa ◽  
Bacterial Isolates ◽  
Leaf Extracts ◽  
In Vitro Antimicrobial Activity ◽  
Significant Difference ◽  
Ethanolic Leaf Extract

Purpose: The study aims to assess the antimicrobial activity of ethanolic leaf extracts of Hibiscus asper and Hibiscus sabdariffa against eight bacterial isolates. Materials and Methods:  An in vitro Antimicrobial activity of ethanolic leaf extract of the two plants against eight nosocomical and pathogenic bacteria viz; Pseudomonas aeruginosa (PAE), Proteus vulgaris (PVU), Klebsiella aerogenes (KAE), Staphylococcus aureus (SAU), Bacillus cereus (BCE), Escherichia coli (ECO), Moraxella catarrhalis (MCA) and Salmonella typhi (STY) was carried out using agar well diffusion assay with the concentration range of 3.13 – 100 mg/mL. Results: H. asper and H. sabdariffa showed significant difference (p< 0.05) in antimicrobial activity against BCE over the rest of the isolates. Inhibition zone diameters exhibited by the isolates to ethanolic leaf extract of H. asper was in descending order of BCE (15.00 ± 1.00a) >ECO (11.67 ± 0.58b) >SAU (7.67 ± 0.58c) >PAE (6.67 ± 0.58d) >STY (5.67 ± 0.58e) while that of H. sabdariffa was in the order BCE (15.33 ± 1.15a) > MCA (11.33 ± 1.15b) > SAU (11.00 ± 1.00bc) > KAE (9.67 ± 0.58c) > PAE (8.00 ± 1.00d) >PVU (7.67 ± 0.57e). PVU, KAE and MCA were resistant to the extract of H. asper while only STY was resistant to that of H. sabdariffa. Conclusion: H. sabdariffa extract demonstrated higher antimicrobial activity against the selected bacterial isolates than H. asper. However, the two extracts minimum inhibition concentrations (MICs) ranged from 25 mg/mL to 12.5 mg/mL. This is worthy of further exploration by pharmacological industries in the formulation of potent broad spectrum antibiotics for combating the present health challenge due to antimicrobial resistance.

scholarly journals In vitro antimicrobial activity of artemisia annua leaf extracts against pathogenic bacteria

2014 ◽  
Vol 6 ◽  
pp. 93-97 ◽  
Author(s):  
Ahmad Tajehmiri ◽  
Fahimeh Issapour ◽  
Mina Nasiri Moslem ◽  
Maryam Tavakoli Lakeh ◽  
Masoud Hassani Kolavani
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Artemisia Annua ◽  
Leaf Extracts ◽  
In Vitro Antimicrobial Activity

scholarly journals In Vitro Antimicrobial Activity of Zodia (Evodia suaveolens) Leaf Extract on Pathogenic Agents Dragon Fruit Plant

Jurnal Biota ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 78-85
Author(s):  
Trio Ageng Prayitno ◽  
Nuril Hidayati
Keyword(s):  
Antimicrobial Activity ◽  
Leaf Extract ◽  
Plant Pathogens ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Leaf Extracts ◽  
Dragon Fruit ◽  
Fruit Plants ◽  
Pathogenic Agents

The use of antimicrobials from plant extracts has not been used optimally to control pathogenic agents in dragon fruit plants. The purpose of this research was to determine the antimicrobial activity of zodia (Evodia suaveolens) leaf extracts on pathogenic agents of dragon fruit plants in vitro. The research method is laboratory research with Completely Randomized Design (CRD). The antimicrobial concentrations of zodia (E. suaveolens) leaf extract used six types including 50%, 60%, 70%, 80%, 90%, and 100% with four replications. The research sample was the leaf of zodia (E. suaveolens), Pseudomonas aeruginosa and Fusarium oxysporum strain Malang. Test the antimicrobial activity of zodia (E. suaveolens) leaf extracts on the growth of P. aeruginosa and F. oxysporum using the disc-diffusion method and wells method. The research instrument was used the observation sheet of the diameter of inhibition zone indicated by the clear zone. The diameter of inhibition zone data were analyzed using the One Way ANOVA test. The results showed that the antimicrobial activity of zodia (E. suaveolens) leaf extract significantly inhibited the growth of P. aeruginosa and F. oxysporum (P <0.05). These results recommend zodia (E. suaveolens) leaf extract as an antimicrobial agent for dragon fruit plant pathogens.

IN VITRO ANTIMICROBIAL ACTIVITY OF CYNODON DACTYLON (L.) PERS. LEAF EXTRACTS. ANTIMICROBIAL ACTIVITY BY ZONE OF INHIBITION ESTIMATION

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (04) ◽  
pp. 37-41
Author(s):  
S. M Pawaskar ◽  
◽  
K. C. Sasangan
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Cynodon Dactylon ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
In Vitro Antimicrobial Activity

The present study was undertaken to evaluate in vitro antimicrobial activity of the successive leaf extracts of Cynodon dactylon in petroleum ether, ethyl acetate, acetone, ethanol, methanol and water, against various gram positive & gram negative bacterial strains using zone of inhibition. Both Agar well diffusion method & Agar disc diffusion method were used to evaluate the antibacterial efficacy. The microorganisms used in the test were – Escherichia coli, Proteus vulgaris, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Shigella flexneri, Salmonella typhi, Salmonella paratyphi A, Salmonella paratyphi B, Bacillus subtilis, Streptococcus pyogenes, Vibrio cholerac and Enterobacter aerogenes. Two fungal strains - Candida albicans and Saccharomyces cerevisiae were also tested to evaluate the antifungal potential of the said plant extract. The reference antibiotics chloramphenicol & ampicillin (antibacterial); nystatin & clotrimazole and (antifungal) were also tested against these standard microorganisms used in the assay and the results were compared with that of the plant extracts.The results of the study revealed that all the seven successive extracts of the leaf powder of Cynodon dactylon ( L.) Pers. exhibited prominent antimicrobial and antifungal activity against all microorganisms used in the study. The nonpolar extracts i.e. petroleum ether, ethyl acetate and acetone showed the most significant antibacterial and antifungal activity against all tested organisms. The petroleum ether and ethyl acetate extracts showing maximum inhibition in the range of 8 mm - 15 mm. This was closely followed by, acetone extract, which showed the inhibition in the range of 9 mm - 13 mm.

scholarly journals In vitro antimicrobial activity of leaf extract of Berlina grandiflora Hutch. and Dalz.

2011 ◽  
Vol 5 (11) ◽  
pp. 1358-1360 ◽  
Author(s):  
G C Akuodor ◽  
S C Onyewenjo ◽  
N A Anyalewechi ◽  
A D Akpan J L Essien ◽  
D O Okoroafor ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Leaf Extract ◽  
In Vitro Antimicrobial Activity

scholarly journals Chemical characterization and in vitro antimicrobial activity of honeybee propolis and Scaptotrigona jujuyensis geopropolis against tomato pathogenic bacteria

2020 ◽  
Vol 42 (5) ◽  
pp. 1799-1808
Author(s):  
Irene Laura Cibanal ◽  
◽  
Leticia Andrea Fernández ◽  
Giovanni Galietta Positano ◽  
Lucía Bóffano Chebataroff ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Chemical Characterization ◽  
In Vitro Antimicrobial Activity

scholarly journals In Vitro Antimicrobial Activity of a New Cephalosporin, Ceftaroline, and Determination of Quality Control Ranges for MIC Testing

2008 ◽  
Vol 53 (3) ◽  
pp. 1271-1274 ◽  
Author(s):  
Steven D. Brown ◽  
Maria M. Traczewski
Keyword(s):  
Antimicrobial Activity ◽  
Quality Control ◽  
Disk Diffusion ◽  
Bacterial Isolates ◽  
In Vitro Antimicrobial Activity ◽  
Reference Strains ◽  
Phenotypic Groups

ABSTRACT The spectrum of activity of ceftaroline was evaluated against 1,247 bacterial isolates representing 44 different species or phenotypic groups. For the majority of species, the activity of ceftaroline was comparable or superior to that of ceftriaxone. MIC and/or disk diffusion quality control ranges of ceftaroline were determined for five standard ATCC reference strains.

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