Characterization of serum protein fractions of dogs naturally infected with Ehrlichia canis or Anaplasma platys associated with uveitis

Ticks are vectors of different types of viruses, protozoans, and other microorganisms, which include Gram-negative prokaryotes of the genera Rickettsiales, Ehrlichia, Anaplasma, and Borrelia. Canine monocytic ehrlichiosis caused by Ehrlichia canis and canine cyclic thrombocytopenia caused by Anaplasma platys are of veterinary importance worldwide. In Myanmar, there is limited information concerning tick-borne pathogens, Ehrlichia and Anaplasma spp., as well as genetic characterization of these species. We performed nested PCR for the gltA gene of the genus Ehrlichia spp. and the 16S rRNA gene of the genus Anaplasma spp. with blood samples from 400 apparently healthy dogs in Nay Pyi Taw area. These amplicon sequences were compared with other sequences from GenBank. Among the 400 blood samples from dogs, 3 (0.75%) were positive for E. canis and 1 (0.25%) was positive for A. platys. The partial sequences of the E. canis gltA and A. platys 16SrRNA genes obtained were highly similar to E. canis and A. platys isolated from different other countries.

Download Full-text

Molecular detection and characterization of Anaplasma platys and Ehrlichia canis in dogs from northern Colombia

Veterinary Microbiology ◽

10.1016/j.vetmic.2019.05.002 ◽

2019 ◽

Vol 233 ◽

pp. 184-189 ◽

Cited By ~ 2

Author(s):

Risa Pesapane ◽

Janet Foley ◽

Richard Thomas ◽

Lyda R. Castro

Keyword(s):

Molecular Detection ◽

Ehrlichia Canis ◽

Anaplasma Platys

Download Full-text

Molecular identification and characterization of Anaplasma platys and Ehrlichia canis in dogs in Mexico

Ticks and Tick-borne Diseases ◽

10.1016/j.ttbdis.2015.11.002 ◽

2016 ◽

Vol 7 (2) ◽

pp. 276-283 ◽

Cited By ~ 22

Author(s):

Consuelo Almazán ◽

Vicente H. González-Álvarez ◽

Isabel G. Fernández de Mera ◽

Alejandro Cabezas-Cruz ◽

Rafael Rodríguez-Martínez ◽

...

Keyword(s):

Molecular Identification ◽

Ehrlichia Canis ◽

Anaplasma Platys ◽

Identification And Characterization

Download Full-text

Molecular characterization of Thai Ehrlichia canis and Anaplasma platys strains detected in dogs

Infection Genetics and Evolution ◽

10.1016/j.meegid.2007.06.002 ◽

2008 ◽

Vol 8 (4) ◽

pp. 433-438 ◽

Cited By ~ 33

Author(s):

Danai Pinyoowong ◽

Sathaporn Jittapalapong ◽

Fanan Suksawat ◽

Roger W. Stich ◽

Arinthip Thamchaipenet

Keyword(s):

Molecular Characterization ◽

Ehrlichia Canis ◽

Anaplasma Platys

Download Full-text

Studies on Parturient Paresis in Dairy Cows. V. On the Composition and Calcium Binding Capacity of Two Bovine Serum Protein Fractions, with Special Regard to Parturient Paresis

Acta Veterinaria Scandinavica ◽

10.1186/bf03548006 ◽

1970 ◽

Vol 11 (1) ◽

pp. 89-102

Author(s):

Gunnar Carlström

Keyword(s):

Dairy Cows ◽

Serum Protein ◽

Calcium Binding ◽

Bovine Serum ◽

Binding Capacity ◽

Protein Fractions ◽

Special Regard ◽

Parturient Paresis ◽

Bovine Serum Protein

Download Full-text

Characterization of the Major Antigenic Protein 2 of Ehrlichia canis and Ehrlichia chaffeensis and Its Application for Serodiagnosis of Ehrlichiosis

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.10.4.520-524.2003 ◽

2003 ◽

Vol 10 (4) ◽

pp. 520-524 ◽

Cited By ~ 7

Author(s):

Tamece T. Knowles ◽

A. Rick Alleman ◽

Heather L. Sorenson ◽

David C. Marciano ◽

Edward B. Breitschwerdt ◽

...

Keyword(s):

Blot Analysis ◽

Single Copy ◽

Ehrlichia Canis ◽

Specific Method ◽

Ehrlichia Chaffeensis ◽

Antigenic Protein ◽

Canine Monocytic Ehrlichiosis ◽

Copy Gene ◽

Species Specific

ABSTRACT Canine monocytic ehrlichiosis, caused by Ehrlichia canis or Ehrlichia chaffeensis, can result in clinical disease in naturally infected animals. Coinfections with these agents may be common in certain areas of endemicity. Currently, a species-specific method for serological diagnosis of monocytic ehrlichiosis is not available. Previously, we developed two indirect enzyme-linked immunosorbent assays (ELISAs) using the major antigenic protein 2 (MAP2) of E. chaffeensis and E. canis. In this study, we further characterized the conservation of MAP2 among various geographic isolates of each organism and determined if the recombinant MAP2 (rMAP2) of E. chaffeensis would cross-react with E. canis-infected dog sera. Genomic Southern blot analysis using digoxigenin-labeled species-specific probes suggested that map2 is a single-copy gene in both Ehrlichia species. Sequences of the single map2 genes of seven geographically different isolates of E. chaffeensis and five isolates of E. canis are highly conserved among the various isolates of each respective ehrlichial species. ELISA and Western blot analysis confirmed that the E. chaffeensis rMAP2 failed to serologically differentiate between E. canis and E. chaffeensis infections.

Download Full-text