scholarly journals Adhesion Conditions of Bifidobacterium pseudocatenulatum KAKii to Human Enterocyte-like Caco-2 Cell Lines

2018 ◽  
Vol 17 (2) ◽  
pp. 96-102
Author(s):  
Mizanurfakhri GHAZALI ◽  
Nurul Wahida SHOKHIMI ◽  
Mazatulikhma MAT ZAIN ◽  
Khalilah ABDUL KHALIL
Keyword(s):  
Cell Surface ◽  
Cell Lines ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Intestinal Cell ◽  
Cell Hydrophobicity ◽  
Intestinal Cell Line ◽  
Significant Difference ◽  
Human Enterocyte ◽  
Attachment Ability

Attachment ability of bifidobacteria strains to the human intestinal surface is an important criterion as a probiotic candidate. However, attachment activity is influenced by external and internal conditions. This study was conducted to screen cell surface hydrophobicity and adhesion scores of bifidobacteria strains. Attachment conditions (pH and exposure time) of selected strains to human enterocyte-like Caco-2 cell lines were subsequently investigated. Three different solvents (n-hexadecane, Toluene, and Xylene) were used in cell surface hydrophobicity analysis. Based on the results obtained, xylene presented consistent cell hydrophobicity activity in all strains used. Bifidobacterium pseudocatenulatum KAKii (wild type strain) gave promising cell hydrophobicity activity with no significant difference (p > 0.05) when compared to Lactobacillus plantarum NBRC 3070 with xylene as a solvent, and also presented a significantly higher attachment score (p < 0.05) compared to all strains used. The influence of pH and time exposure on adhesion of B. pseudocatenulatum KAKii to Caco-2 cells revealed that this strain was favored to attach to the intestinal cell line at pH 6 and after 120 min of exposure. Further optimization of attachment conditions will be carried out.

scholarly journals Effect of Eugenol on Cell Surface Hydrophobicity, Adhesion, and Biofilm ofCandida tropicalisandCandida dubliniensisIsolated from Oral Cavity of HIV-Infected Patients

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Suelen Balero de Paula ◽  
Thais Fernanda Bartelli ◽  
Vanessa Di Raimo ◽  
Jussevania Pereira Santos ◽  
Alexandre Tadachi Morey ◽  
...  
Keyword(s):  
Cell Surface ◽  
Oral Cavity ◽  
Biofilm Formation ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Planktonic Cells ◽  
Hydrophobic Behavior ◽  
Significant Difference ◽  
Substrate Surfaces ◽  
Sessile Cells

MostCandidaspp. infections are associated with biofilm formation on host surfaces. Cells within these communities display a phenotype resistant to antimicrobials and host defenses, so biofilm-associated infections are difficult to treat, representing a source of reinfections. The present study evaluated the effect of eugenol on the adherence properties and biofilm formation capacity ofCandida dubliniensisandCandida tropicalisisolated from the oral cavity of HIV-infected patients. All isolates were able to form biofilms on different substrate surfaces. Eugenol showed inhibitory activity against planktonic and sessile cells ofCandidaspp. No metabolic activity in biofilm was detected after 24 h of treatment. Scanning electron microscopy demonstrated that eugenol drastically reduced the number of sessile cells on denture material surfaces. MostCandidaspecies showed hydrophobic behavior and a significant difference in cell surface hydrophobicity was observed after exposure of planktonic cells to eugenol for 1 h. Eugenol also caused a significant reduction in adhesion of mostCandidaspp. to HEp-2 cells and to polystyrene. These findings corroborate the effectiveness of eugenol againstCandidaspecies other thanC. albicans, reinforcing its potential as an antifungal applied to limit both the growth of planktonic cells and biofilm formation on different surfaces.

scholarly journals Evaluation of cell surface hydrophobicity and biofilm formation as pathogenic determinants among ESBL producing uropathogenic Escherichia coli

2021 ◽  
Vol 8 (4) ◽  
pp. 263-267
Author(s):  
Anandkumar H ◽  
Amaresh Nigudgi ◽  
Vinay Hajare ◽  
Sunil Biradar
Keyword(s):  
Cell Surface ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Critical Role ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Adverse Outcomes ◽  
Biofilm Production ◽  
Significant Difference ◽  
Esbl Producers

The attachment of the bacteria to the host cell and ability to invade the cell are regarded as important steps in the infectious process. The hydrophobicity of the microbial surface plays a critical role in the adherence of bacteria to the surface. The ability of biofilm formation can increase survival chance of microorganism, as cell growing in biofilm are highly resistant to the components of the immune system and many antimicrobial agents. Infection caused by ESBL- producers are associated with severe adverse outcomes and may be related to increased virulence of these strains.: A total of 100 urinary were selected for the study, of which 50 strains were from ESBL producers and 50 from non- ESBL-producing uropathogenic(UPEC) strains. The urinary isolates that were resistant to at least one of the three indicator cephalosporins (cefotaxime, cefpodoxime and ceftazidime) were tested for ESBL production by quantitative E-strip method. All the 100 urinary strains were tested for cell surface hydrophobicity (CSH) by salt aggregation method and Biofilm production by tissue culture plate method.Among ESBL producers, 19 (38%) were CSH positive and 34 (68%) were biofilm producers. However among non-ESBL producers, 05 (10%) were CSH positive and 12 (24%) were biofilm producers. Statistically significant difference (&#60;0.001) was seen in the occurrence of CSH and biofilm production between ESBL and non ESBL producing UPEC isolates.In the present study, it was found that the ESBL producing isolates had a higher ability to form biofilm and CSH; both of them are among the important virulence factors associated with cell surface adherence which is the first step in bacterial infection.

Autecology of scum producing bacteria

1998 ◽  
Vol 37 (4-5) ◽  
pp. 527-530 ◽  
Author(s):  
Hilde Lemmer ◽  
George Lind ◽  
Margit Schade ◽  
Birgit Ziegelmayer
Keyword(s):  
Wastewater Treatment ◽  
Cell Surface ◽  
Wastewater Treatment Plants ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Residence Times ◽  
Loading Conditions ◽  
Emulsifying Activity ◽  
Substrate Preferences

Non-filamentous hydrophobic scum bacteria were isolated from scumming wastewater treatment plants (WWTP) by means of adhesion to hydrocarbons. They were characterized with respect to taxonomy, substrate preferences, cell surface hydrophobicity, and emulsification capability. Their role during flotation events is discussed. Rhodococci are selected by hydrolysable substrates and contribute to flotation both by cell surface hydrophobicity and emulsifying activity at long mean cell residence times (MCRT). Saprophytic Acinetobacter strains are able to promote flotation by hydrophobicity and producing emulsifying agents under conditions when hydrophobic substrates are predominant. Hydrogenophaga and Acidovorax species as well as members of the Cytophaga/Flavobacterium group are prone to proliferate under low loading conditions and contribute to flotation mainly by emulsification.

Phenol and n-alkanes (C12 and C16) utilization: influence on yeast cell surface hydrophobicity

2008 ◽  
Vol 24 (9) ◽  
pp. 1943-1949 ◽  
Author(s):  
Łukasz Chrzanowski ◽  
Katarzyna Bielicka-Daszkiewicz ◽  
Mikołaj Owsianiak ◽  
Andreas Aurich ◽  
Ewa Kaczorek ◽  
...  
Keyword(s):  
Cell Surface ◽  
Yeast Cell ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Yeast Cell Surface
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