Evaluation of cell surface hydrophobicity and biofilm formation as pathogenic determinants among ESBL producing uropathogenic Escherichia coli

The attachment of the bacteria to the host cell and ability to invade the cell are regarded as important steps in the infectious process. The hydrophobicity of the microbial surface plays a critical role in the adherence of bacteria to the surface. The ability of biofilm formation can increase survival chance of microorganism, as cell growing in biofilm are highly resistant to the components of the immune system and many antimicrobial agents. Infection caused by ESBL- producers are associated with severe adverse outcomes and may be related to increased virulence of these strains.: A total of 100 urinary were selected for the study, of which 50 strains were from ESBL producers and 50 from non- ESBL-producing uropathogenic(UPEC) strains. The urinary isolates that were resistant to at least one of the three indicator cephalosporins (cefotaxime, cefpodoxime and ceftazidime) were tested for ESBL production by quantitative E-strip method. All the 100 urinary strains were tested for cell surface hydrophobicity (CSH) by salt aggregation method and Biofilm production by tissue culture plate method.Among ESBL producers, 19 (38%) were CSH positive and 34 (68%) were biofilm producers. However among non-ESBL producers, 05 (10%) were CSH positive and 12 (24%) were biofilm producers. Statistically significant difference (<0.001) was seen in the occurrence of CSH and biofilm production between ESBL and non ESBL producing UPEC isolates.In the present study, it was found that the ESBL producing isolates had a higher ability to form biofilm and CSH; both of them are among the important virulence factors associated with cell surface adherence which is the first step in bacterial infection.

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Effect of Eugenol on Cell Surface Hydrophobicity, Adhesion, and Biofilm ofCandida tropicalisandCandida dubliniensisIsolated from Oral Cavity of HIV-Infected Patients

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/505204 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 20

Author(s):

Suelen Balero de Paula ◽

Thais Fernanda Bartelli ◽

Vanessa Di Raimo ◽

Jussevania Pereira Santos ◽

Alexandre Tadachi Morey ◽

...

Keyword(s):

Cell Surface ◽

Oral Cavity ◽

Biofilm Formation ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Planktonic Cells ◽

Hydrophobic Behavior ◽

Significant Difference ◽

Substrate Surfaces ◽

Sessile Cells

MostCandidaspp. infections are associated with biofilm formation on host surfaces. Cells within these communities display a phenotype resistant to antimicrobials and host defenses, so biofilm-associated infections are difficult to treat, representing a source of reinfections. The present study evaluated the effect of eugenol on the adherence properties and biofilm formation capacity ofCandida dubliniensisandCandida tropicalisisolated from the oral cavity of HIV-infected patients. All isolates were able to form biofilms on different substrate surfaces. Eugenol showed inhibitory activity against planktonic and sessile cells ofCandidaspp. No metabolic activity in biofilm was detected after 24 h of treatment. Scanning electron microscopy demonstrated that eugenol drastically reduced the number of sessile cells on denture material surfaces. MostCandidaspecies showed hydrophobic behavior and a significant difference in cell surface hydrophobicity was observed after exposure of planktonic cells to eugenol for 1 h. Eugenol also caused a significant reduction in adhesion of mostCandidaspp. to HEp-2 cells and to polystyrene. These findings corroborate the effectiveness of eugenol againstCandidaspecies other thanC. albicans, reinforcing its potential as an antifungal applied to limit both the growth of planktonic cells and biofilm formation on different surfaces.

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SubMICs of penicillin and erythromycin enhance biofilm formation and hydrophobicity of Corynebacterium diphtheriae strains

Journal of Medical Microbiology ◽

10.1099/jmm.0.052373-0 ◽

2013 ◽

Vol 62 (5) ◽

pp. 754-760 ◽

Cited By ~ 18

Author(s):

D. L. R. Gomes ◽

R. S. Peixoto ◽

E. A. B. Barbosa ◽

F. Napoleão ◽

P. S. Sabbadini ◽

...

Keyword(s):

Cell Surface ◽

Surface Properties ◽

Biofilm Formation ◽

Antimicrobial Agents ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Corynebacterium Diphtheriae ◽

Surface Charges ◽

Bacterial Surface ◽

Bacterial Morphology

Subinhibitory concentrations (subMICs) of antibiotics may alter bacterial surface properties and change microbial physiology. This study aimed to investigate the effect of a subMIC (⅛ MIC) of penicillin (PEN) and erythromycin (ERY) on bacterial morphology, haemagglutinating activity, cell-surface hydrophobicity (CSH) and biofilm formation on glass and polystyrene surfaces, as well as the distribution of cell-surface acidic anionic residues of Corynebacterium diphtheriae strains (HC01 tox − strain; CDC-E8392 and 241 tox + strains). All micro-organisms tested were susceptible to PEN and ERY. Growth in the presence of PEN induced bacterial filamentation, whereas subMIC of ERY caused cell-size reduction of strains 241 and CDC-E8392. Adherence to human erythrocytes was reduced after growth in the presence of ERY, while CSH was increased by a subMIC of both antibiotics in bacterial adherence to n-hexadecane assays. Conversely, antibiotic inhibition of biofilm formation was not observed. All strains enhanced biofilm formation on glass after treatment with ERY, while only strain 241 increased glass adherence after cultivation in the presence of PEN. Biofilm production on polystyrene surfaces was improved by ⅛ MIC of ERY. After growth in the presence of both antimicrobial agents, strains 241 and CDC-E8392 exhibited anionic surface charges with focal distribution. In conclusion, subMICs of PEN and ERY modified bacterial surface properties and enhanced not only biofilm formation but also cell-surface hydrophobicity. Antibiotic-induced biofilm formation may contribute to the inconsistent success of antimicrobial therapy for C. diphtheriae infections.

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Cell surface hydrophobicity, biofilm formation, adhesives properties and molecular detection of adhesins genes in Staphylococcus aureus associated to dental caries

Microbial Pathogenesis ◽

10.1016/j.micpath.2010.03.007 ◽

2010 ◽

Vol 49 (1-2) ◽

pp. 14-22 ◽

Cited By ~ 58

Author(s):

Bochra Kouidhi ◽

Tarek Zmantar ◽

Hajer Hentati ◽

Amina Bakhrouf

Keyword(s):

Staphylococcus Aureus ◽

Dental Caries ◽

Cell Surface ◽

Biofilm Formation ◽

Molecular Detection ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity

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Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease

Scientific Reports ◽

10.1038/s41598-020-74977-y ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Zhiyan He ◽

Xu Zhang ◽

Zhongchen Song ◽

Lu Li ◽

Haishuang Chang ◽

...

Keyword(s):

Cell Surface ◽

Periodontal Disease ◽

Biofilm Formation ◽

Cell Structure ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Laser Scanning Microscopy ◽

Confocal Laser ◽

Antimicrobial Effects ◽

Virulence Properties

Abstract Porphyromonas gingivalis is a causative agent in the onset and progression of periodontal disease. This study aims to investigate the effects of quercetin, a natural plant product, on P. gingivalis virulence properties including gingipain, haemagglutinin and biofilm formation. Antimicrobial effects and morphological changes of quercetin on P. gingivalis were detected. The effects of quercetin on gingipains activities and hemolytic, hemagglutination activities were evaluated using chromogenic peptides and sheep erythrocytes. The biofilm biomass and metabolism with different concentrations of quercetin were assessed by the crystal violet and MTT assay. The structures and thickness of the biofilms were observed by confocal laser scanning microscopy. Bacterial cell surface properties including cell surface hydrophobicity and aggregation were also evaluated. The mRNA expression of virulence and iron/heme utilization was assessed using real time-PCR. Quercetin exhibited antimicrobial effects and damaged the cell structure. Quercetin can inhibit gingipains, hemolytic, hemagglutination activities and biofilm formation at sub-MIC concentrations. Molecular docking analysis further indicated that quercetin can interact with gingipains. The biofilm became sparser and thinner after quercetin treatment. Quercetin also modulate cell surface hydrophobicity and aggregation. Expression of the genes tested was down-regulated in the presence of quercetin. In conclusion, our study demonstrated that quercetin inhibited various virulence factors of P. gingivalis.

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Factors associated with adherence to and biofilm formation on polystyrene byStenotrophomonas maltophilia: the role of cell surface hydrophobicity and motility

FEMS Microbiology Letters ◽

10.1111/j.1574-6968.2008.01292.x ◽

2008 ◽

Vol 287 (1) ◽

pp. 41-47 ◽

Cited By ~ 94

Author(s):

Arianna Pompilio ◽

Raffaele Piccolomini ◽

Carla Picciani ◽

Domenico D'Antonio ◽

Vincenzo Savini ◽

...

Keyword(s):

Cell Surface ◽

Biofilm Formation ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Factors Associated

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Biofilm Formation and Cell Surface Properties among Pathogenic and Nonpathogenic Strains of the Bacillus cereus Group

Applied and Environmental Microbiology ◽

10.1128/aem.00155-09 ◽

2009 ◽

Vol 75 (20) ◽

pp. 6616-6618 ◽

Cited By ~ 81

Author(s):

Sandrine Auger ◽

Nalini Ramarao ◽

Christine Faille ◽

Agnès Fouet ◽

Stéphane Aymerich ◽

...

Keyword(s):

Epithelial Cells ◽

Cell Surface ◽

Bacillus Cereus ◽

Digestive Tract ◽

Biofilm Formation ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Bacillus Cereus Group ◽

Cell Surface Properties ◽

Tract Infections

ABSTRACT Biofilm formation by 102 Bacillus cereus and B. thuringiensis strains was determined. Strains isolated from soil or involved in digestive tract infections were efficient biofilm formers, whereas strains isolated from other diseases were poor biofilm formers. Cell surface hydrophobicity, the presence of an S layer, and adhesion to epithelial cells were also examined.

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