scholarly journals Mobile Phone Electromagnetic Waves and Its Effect on Human Ejaculated Semen: An in vitro Study

2012 ◽  
Vol 3 (1) ◽  
pp. 15-21 ◽  
Author(s):  
SS Vasan ◽  
Srinivas Belur Veerachari
Keyword(s):  
Mobile Phone ◽  
Dna Fragmentation ◽  
Mobile Phones ◽  
Electromagnetic Waves ◽  
In Vitro Study ◽  
Human Semen ◽  
Fragmentation Index ◽  
Increasing Demand ◽  
Dna Fragmentation Index

ABSTRACT Mobile phones usage has seen an exponential growth recently. With this increasing demand, the amount of electromagnetic radiation (EMR) exposed is also increasing. Hence, we studied the effect of these radiations on ejaculated human semen and speculate the contribution of these harmful radiations in male infertility. Samples exposed to EMR showed a significant decrease in sperm motility and viability, increase in reactive oxygen species (ROS) and DNA fragmentation index (DFI) compared to unexposed group. We concluded that mobile phones emit electromagnetic waves which lead to oxidative stress in human semen and also cause changes in DNA fragmentation. We extrapolate these findings to speculate that these radiations may negatively affect spermatozoa and impair male fertility. How to cite this article Veerachari SB, Vasan SS. Mobile Phone Electromagnetic Waves and Its Effect on Human Ejaculated Semen: An in vitro Study. Int J Infertility Fetal Med 2012;3(1):15-21.

scholarly journals Synergistic Effect of Radiofrequency Electromagnetic Fields of Dental Light Cure Devices and Mobile Phones Accelerating Microleakage of Amalgam Restorations: An in vitro Study

2019 ◽  
Vol 9 (2Apr) ◽  
Author(s):  
S M J Mortazavi ◽  
A Dehghani Nazhvani ◽  
M Paknahad
Keyword(s):  
Mobile Phone ◽  
Electromagnetic Fields ◽  
Mobile Phones ◽  
In Vitro Study ◽  
Control Group ◽  
Human Teeth ◽  
Fourth Group ◽  
Mobile Phone Radiation ◽  
Amalgam Restorations

Background: Previous studies have shown that exposure to electromagnetic fields produced by magnetic resonance imaging or mobile phones can lead to increased microleakage of dental amalgam.Objective: The aim of the present study was to investigate the effect of electromagnetic field of a commercial dental light cure device and a common GSM mobile phone on microleakage of amalgam restorations.Materials and Methods: Identical class V cavities were prepared on the buccal surfaces of 60 non-carious extracted human teeth. The samples were randomly divided into 4 groups of 20 samples each. The samples in the first group were not exposed to electromagnetic fields, while the second and the third groups were exposed to electromagnetic fields produced by a commercial light cure device, or mobile phone radiation (60 min), respectively. The fourth group was exposed to electromagnetic radiations emitted by both mobile phone for 60 min and light cure device. Then, teeth samples were scored for microleakage according to a standard dye penetration protocol by examination under a stereomicroscope.Results: The mean score of microleakage in the fourth group (light cure + mobile phone) was significantly higher than that of the control group (P =0.030). Moreover, the scores of microleakage in this group were significantly higher than that of the second group (light cure only) (P= 0.043).Conclusion: Exposure of amalgam restorations to electromagnetic fields produced by both light cure devices and mobile phones can synergistically increase the microleakage of amalgam restorations.

scholarly journals DNA fragmentation index, pAKT and pERK1/2 in cumulus cells are related to oocyte competence in patients undergoing in vitro fertilization programme

Zygote ◽  
2019 ◽  
Vol 27 (05) ◽  
pp. 350-354
Author(s):  
Giovanni Ruvolo ◽  
Maria Carmela Roccheri ◽  
Claudio Luparello ◽  
Domenica Matranga ◽  
Alberto Ferrigno ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Dna Fragmentation ◽  
Nuclear Localization ◽  
Inverse Relationship ◽  
Cumulus Cells ◽  
Intracellular Accumulation ◽  
Significant Inverse Relationship ◽  
Fragmentation Index ◽  
Dna Fragmentation Index

SummaryActivated pERK1/2 and pAKT are key players in supporting cell survival and proliferation pathways. Translocation of pERK1/2 into the nucleus, where it interacts with transcription factors and DNA itself, is instrumental in exerting an anti-apoptotic effect. In this study, pAKT levels, pERK1/2 nuclear localization and DNA fragmentation index (DFI) in cumulus cells of single cumulus–oocyte complexes of patients undergoing in vitro fertilization programmes were evaluated and correlated with the clinical outcome of the related embryos. For a positive clinical outcome of blastocyst development, pERK1/2 nuclear localization and DFI value had a significant inverse relationship, whereas the former and the intracellular accumulation of pAKT had a significant direct relationship. This relationship was not observed for the negative clinical outcome of the arrested embryos. Moreover, intracellular accumulation of pAKT and DFI value had a significant inverse relationship in all samples examined. The obtained data suggest that the intranuclear relocation of pERK1/2, along with an enhanced intracellular accumulation of pAKT, may exert a survival effect and increase cell viability, therefore providing a novel marker tool to choose the best oocyte to be fertilized and submitted to an intracytoplasmic sperm injection cycle.

scholarly journals Efficient and selective catalytic hydroxylation of unsaturated plant oils: a novel method for producing anti-pathogens

BMC Chemistry ◽  
2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Ahmed M. Senan ◽  
Binru Yin ◽  
Yaoyao Zhang ◽  
Mustapha M. Nasiru ◽  
Yong‐Mei Lyu ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
In Vitro Study ◽  
Functional Method ◽  
Plant Oils ◽  
Main Process ◽  
Catalytic Method ◽  
Novel Method ◽  
Pharmaceutical Industries ◽  
Increasing Demand

AbstractWith the increasing demand for antimicrobial agents and the spread of antibiotic resistance in pathogens, the exploitation of plant oils to partly replace antibiotic emerges as an important source of fine chemicals, functional food utility and pharmaceutical industries. This work introduces a novel catalytic method of plant oils hydroxylation by Fe(III) citrate monohydrate (Fe3+-cit.)/Na2S2O8 catalyst. Methyl (9Z,12Z)-octadecadienoate (ML) was selected as an example of vegetable oils hydroxylation to its hydroxy-conjugated derivatives (CHML) in the presence of a new complex of Fe(II)-species. Methyl 9,12-di-hydroxyoctadecanoate 1, methyl-9-hydroxyoctadecanoate 2 and methyl (10E,12E)-octadecanoate 3 mixtures is produced under optimized condition with oxygen balloon. The specific hydroxylation activity was lower in the case of using Na2S2O8 alone as a catalyst. A chemical reaction has shown the main process converted of plantoils hydroxylation and (+ 16 Da) of OH- attached at the methyl linoleate (ML-OH). HPLC and MALDI-ToF-mass spectrometry were employed for determining the obtained products. It was found that adding oxidizing agents (Na2S2O8) to Fe3+ in the MeCN mixture with H2O would generate the new complex of Fe(II)-species, which improves the C-H activation. Hence, the present study demonstrated a new functional method for better usage of vegetable oils.Producing conjugated hydroxy-fatty acids/esters with better antipathogenic properties. CHML used in food industry, It has a potential pathway to food safety and packaging process with good advantages, fundamental to microbial resistance. Lastly, our findings showed that biological monitoring of CHML-minimum inhibitory concentration (MIC) inhibited growth of various gram-positive and gram-negative bacteria in vitro study. The produced CHML profiles were comparable to the corresponding to previousstudies and showed improved the inhibition efficiency over the respective kanamycin derivatives.

scholarly journals Correlation between the DNA fragmentation index (DFI) and sperm morphology of infertile patients

2021 ◽  
Author(s):  
Alberto Ferrigno ◽  
Giovanni Ruvolo ◽  
Giuseppina Capra ◽  
Nicola Serra ◽  
Liana Bosco
Keyword(s):  
Dna Fragmentation ◽  
Sperm Morphology ◽  
Seminal Fluid ◽  
Image Analyzer ◽  
Fragmented Dna ◽  
Fragmentation Index ◽  
Group A ◽  
Group B ◽  
Infertile Patients ◽  
Dna Fragmentation Index

Abstract Purpose To evaluate the correlation between the DNA Fragmentation Index (DFI) and sperm morphology in patients undergoing ICSI, as a predictive parameter in reproductive outcomes. Methods A retrospective study was conducted on 125 infertile patients enrolled in a fertility clinic. Seminal characteristics were measured following the WHO guidelines (2010) for the examination of the seminal fluid. After collecting motile sperm population by pellet swim up, DFI was calculated and simultaneously associated with sperm morphology using in situ TUNEL assay and an image analyzer software in at least 250 spermatozoa for each patient. Results All subjects were divided into two groups according to a cutoff established, by choice, of the sperm DFI (15%): group A (< 15%) consisting of 65 patients and group B (≥ 15%) of 60 patients. Data were analyzed using non-parametric statistical methods. The results demonstrate that there is no statistical difference between the two groups in seminal characteristics. The collective data show a high significant correlation, suggesting that spermatozoa with abnormal morphology are the best candidates to contain DNA damage (p < 0.001). Also, when group A is compared with group B, an increased percentage of morphologically normal spermatozoa with fragmented DNA was observed in patients, with DFI values ≥ 15% (p < 0.001). Conclusion These results are aimed at providing an exact value of DFI in morphologically normal spermatozoa, which will be helpful to the embryologist in evaluating the risk of transferring, during the ICSI procedure, a spermatozoon whit normal morphology but fragmented DNA.

DETERMINATION OF THE HUMAN SPERM DNA FRAGMENTATION INDEX

Vestnik ◽  
2021 ◽  
pp. 226-232
Author(s):  
К. К. Тлеуханов ◽  
Н. А. Алтыбаева ◽  
М. К. Отарбаев ◽  
Е. М. Тойшибеков ◽  
А. А. Тлеуханова
Keyword(s):  
Dna Fragmentation ◽  
Human Sperm ◽  
Sperm Dna Fragmentation ◽  
Fragmentation Index ◽  
Sperm Dna ◽  
Sperm Dna Fragmentation Index ◽  
Dna Fragmentation Index

В статье представлены собранные данные о методах устранения повышенной частоты фрагментации ДНК у сперматозоидов, которые в некоторых исследованиях подтверждают, что введение антиоксидантов может снизить уровень фрагментации ДНК у сперматозоидов. The article presents collected data on methods of eliminating the increased frequency of DNA fragmentation in spermatozoa, which in some studies confirm that the introduction of antioxidants can reduce the level of DNA fragmentation in spermatozoa.

O-212 MACS vs TESA for raised sperm DNA fragmentation index – a RCT

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
K C Mantravadi ◽  
D R Gedela
Keyword(s):  
Dna Fragmentation ◽  
Live Birth ◽  
Trial Registration ◽  
Sperm Dna Fragmentation ◽  
Sperm Selection ◽  
Reproductive Outcomes ◽  
Fragmentation Index ◽  
Sperm Dna ◽  
Sperm Dna Fragmentation Index ◽  
Dna Fragmentation Index

Abstract Study question In Individuals with raised Sperm DNA Fragmentation Index (SDF), will sperm selection by magnetic activated cell sorting (MACS) or surgical retrieval of testicular sperms (TESA) optimize the reproductive outcomes? Summary answer Couples with failed implantation raised SDF, TESA /MACS offer similar results. This RCT doesn’t prove superiority or added benefit with any of the above interventions. What is known already It is evident that raised SDF negatively affects the reproductive outcomes. Management for raised SDF to optimize reproductive outcomes is still elusive. Study design, size, duration This was a Randomized Control Trial (RCT) with prior approval from institutional Ethical Committee and trial registration. Couples undergoing stimulation with raised SDF were randomized to MACS (n = 75) and TESA (n = 75) for sperm selection between April2019 & February2020. Participants/materials, setting, methods Couples with history of one failed IVF had SDF testing and SDF&gt;30% were recruited. SDF test done with SCSA method and randomized using software. ICSI was the method of insemination. Extended embryo culture till blastocyst was done and freeze all policy was opted. Two Blastocysts that showed 100% survival were transferred in a Frozen Embryo transfer (FET) cycle. Embryonic and Reproductive outcomes were compared between both groups. Live birth and Miscarriage were the primary outcomes. Main results and the role of chance Reproductive Outcomes of MACS Vs TESA were: Average Blastocyst conversion - 32% Vs 39% (RR 1.22, CI1.00 to 1.50) Implantation rate (IR) - 50% Vs 35% (RR - 0.71, CI 0.51 to 0.98) Miscarriage rate (MR) - 5.3% Vs 11% (RR1.6333, CI 0.5227 to 5.1039) Multiple Pregnancy rate (MPR) - 8% Vs 4% Live birth Rate (LBR) per Intention to treat (ITT) - 41.3% Vs 44% (RR 0.95, 95% CI 0.72 to 1.26) LBR per ET cycle - 63% Vs 56% (RR 1.23, 95% CI 0.77 to 1.94) Our preliminary results suggest that despite greater availability of blastocysts for transfer in the TESA group, no difference in ART outcomes was observed between the groups. Though the IR was statistically low with TESA, our primary outcomes LBR and MR were comparable. TESA or MACS seem to offer similar outcomes. Considering the invasiveness with TESA, MACS can be offered for better sperm selection for couples with raised sperm DFI & failed implantation. Limitations, reasons for caution Small sample size. TESA is a surgical intervention Wider implications of the findings Optimal intervention for management of SDF still needs further research. Trial registration number CTRI/2019/07/020140

Sign in / Sign up

Export Citation Format

Share Document