Use of Eye Bank Pre-cut Donor Tissue in DSAEK

Purpose. To determine whether the rate of graft detachment in patients undergoing the DSAEK procedure is influenced by the time elapsed between donor death and tissue implantation. Methods. Data on procedure outcome and donor tissue for patients undergoing the DSAEK procedure were reviewed. Data on time elapsed between harvest, processing, storage, and implantation of the tissue, as well as donor tissue endothelial cell count were obtained from reports made available from the Eye Bank of Canada. The adverse outcome of interest was graft detachment. Results. 71 cases were reviewed, with 14% resulting in detachment. The following time periods were compared between detachment and nondetachment groups: donor death to enucleation; enucleation to processing; duration of storage at the Eye Bank to implantation. No statistically significant differences were found (Student's t-test, ). Endothelial cell counts of donor tissue were compared between the two groups, and no statistically significant difference was found (Student's t-test, ). Conclusion. The range of processing times and endothelial cell counts in donor tissue available from the Eye Bank did not predict a change in the rate of graft dislocation in one surgeon's practice.

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A new storage medium for pre-cut donor tissue for DSAEK in eye bank

Acta Ophthalmologica ◽

10.1111/j.1755-3768.2009.2273.x ◽

2009 ◽

Vol 87 ◽

pp. 0-0 ◽

Cited By ~ 1

Author(s):

A POCOBELLI ◽

D AMATO ◽

P DUCOLI

Keyword(s):

Storage Medium ◽

Eye Bank ◽

Donor Tissue

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Characterization of Corneal Donor Tissue Lesions by Anterior Segment Optical Coherence Tomography Compared With Eye Bank Technician Slit-Lamp Examination

Cornea ◽

10.1097/ico.0000000000001700 ◽

2018 ◽

Vol 37 (10) ◽

pp. 1318-1323

Author(s):

S. Tammy Hsu ◽

Isaac Perry ◽

Sara Botsay ◽

Narae Ko ◽

Sandra S. Stinnett ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Anterior Segment ◽

Optical Coherence ◽

Slit Lamp ◽

Eye Bank ◽

Slit Lamp Examination ◽

Donor Tissue ◽

Corneal Donor ◽

Corneal Donor Tissue

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How Implementing a Quality Management System at the LIONS Eye Bank Saar-Lor-Lux, Trier/Western Palatinate from 2006 to 2016 Impacted the Rate and Reasons for Discarding Human Organ-Cultured Corneas

Klinische Monatsblätter für Augenheilkunde ◽

10.1055/a-1327-3835 ◽

2021 ◽

Author(s):

Daniel Laun ◽

Shady Suffo ◽

Kristina Kramp ◽

Mona Bischoff ◽

Manuela Huber ◽

...

Keyword(s):

Quality Management ◽

Management System ◽

Quality Management System ◽

Iso 9001 ◽

Eye Bank ◽

Human Organ ◽

Donor Tissue ◽

Microsoft Access ◽

Conjunctival Swab ◽

And Training

Abstract Background The aim of this study was to explore how the implementation of the quality management system (QM; according to DIN EN ISO 9001 : 2008) at the LIONS Eye Bank impacted the rate and reasons for discarding donated corneas. The QM system was introduced in November 2010. Material and methods The rate of and reasons for discarding cornea tissues at the LIONS Eye Bank Saar-Lor-Lux,Trier/Western Palatinate from 2006 to 2016 were analysed retrospectively. 4,140 corneas from 2,084 donors were analysed. 1,640 corneas were processed before the QM system had been introduced and 2,500 corneas thereafter. Main reasons for discarding cornea tissues were endothelial quality, contamination of the medium and a positive conjunctival swab. The data was extracted from clinical files and data filing systems, entered into a Microsoft Access database and analysed statistically with SPSS. Outcome Following the implementation of a QM system, the discard rate decreased significantly from 50.1% to 39.7% (p < 0.0001). Prior to the implementation of the QM system, 493 (30.1%) cornea tissues had been discarded due to endothelial quality, while this figure improved significantly to 604 (24.2%) after the QM system had been implemented (p < 0.0001). Contamination was the reason for discarding tissue in 173 (10.5%) cases before implementation of the QM system, and in 124 (5.0%) cases thereafter (p < 0.0001). Positive conjunctival swab was the reason for discarding tissue in 10 (0.6%) cases before the QM system had been implemented, and in 53 (2.1%) cases thereafter (p < 0.0001). Conclusion Implementation of a QM system significantly reduced the rate of discarded donor tissue in the LIONS Eye Bank. Better management of contamination as a reason for discarding cornea tissues can be attributed to improved standards, protocols and training that are part of this QM system.

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AGE BASED SPECULAR MICROSCOPIC EVALUATION OF FIRST 50 CORNEAL TISSUE OBTAINED THROUGH HCRP AT A NEWLY ESTABLISHED EYE BANK IN EASTERN INDIA

10.36106/5101639 ◽

2021 ◽

pp. 41-43

Author(s):

Mamta Singh ◽

Pradeep Karak ◽

Bibhuti P Sinha ◽

Manish kumar

Keyword(s):

Corneal Thickness ◽

P Value ◽

Corneal Tissue ◽

Age Group ◽

Progressive Decrease ◽

Eastern India ◽

Eye Bank ◽

Donor Tissue ◽

Microscopic Evaluation ◽

Significant Difference

Background – Cornea transplantation has an important role in reducing the burden of corneal blindness but availability of corneal donor tissue is a significant limitation. The overall utilization of available cornea is further limited by the quality of donor corneal tissue.Specular microscopic evaluation of donor cornea is emerging as an indispensable tool of endothelial evaluation. Aim – Aim of this study to do an age based specular microscopic evaluation of first 50 corneal tissue obtained through hospital cornea retrieval program at a newly established eye bank in Eastern India Material and method – Total 50 corneo-scleral donor tissue were included in study after excluding the contraindication of cornea donation. Three tissues were could be not included in final assessment due to unavailability of specular microscopic image.47 tissues were divided in different age groups – <20 years (10 tissues),20-40 years (10 tissues),40-60 years (10 tissue) an >60 years (17 tissues). Specular microscopic evaluation was done with Konan eye bank keratoanlyser - CellChek® D® (Konan Medical, Irvine, CA). Endothelial cell density (ECD), corneal thickness (CT), coefficient of variance (CV) and percentage of hexagonal cells (6A) were evaluated and correlated with age. Result – The average age of donor was 47.02 years. The average CT, ECD, CV and 6A were 523.42 +/- 16.37 micron, 2222.87 +/- 514.62 cells/mm2, 35.27 +/- 4.04, 53.06 +/-8.30 % respectively.The difference in ECD was significantly high in age group <20 years than in comparison to those between 20-40 years (p value - 0.018),40-60 years (p value - 0.003) and more than 60 years (p value - 0.0001). There was a progressive decrease in ECD count with age but the change in ECD count between age group 20-40years and 40-60 years was not significant (p value - 0.12).ECD was significantly low in age group > 60 years in comparison to all other group i.e.<20 years (p value – 0.0001), 20-40 years (p value - 0.003) and 40-60 years (p value - 0.016). There was no significant difference in any other parameter (CT, CV, 6A) in any of the age group. Conclusion - Eye bank specular microscope is an important tool in proper evaluation of donor corneo-scleral button. The ECD is significantly high in tissue from younger age of donor with a progressive decrease in age.CCT,CV and 6A are found to have no correlation with age.

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