Bacteriological Examination of Air, Water and Milk

Author(s):  
Nanda Maheshwari
2018 ◽  
pp. 37-40
Author(s):  
Phuong Thao Tien Nguyen ◽  
Anh Hung Tran ◽  
Van Tam Le ◽  
Huu Thao Phung ◽  
Phan Quynh Anh Nguyen

Objective: To evaluate the effect of gamma sterilization and its effect on microscopic structure of deeply frozen dog skull. Subjects and methods: 50 pairs of dog skull fragments have the same size,which were taken symmetrically through the midline of the skull, were divided into two lots. Evident lot consisting of 50 pieces are just deeply frozen, not irradicated. Experimental lot which was composed of 50 pieces were deeply frozen and exposed to 25kGy of gamma radiation. Afterwards, to evaluate the effect of gamma sterilization and compare the alter of bone matrix between lot groups. Results: After gamma irradiation, all dog skull fragments were negative with bacteriological examination. In microscopic scale, no difference was found between the two lots and there was no structural change in the irradiated fragments of all dog skull. Conclusion: Gamma irradiation with 25 kGy: (i) It has the ability to kill bacteria completely in the type of bacteria commonly infected tissue. (ii) It does not alter the structure of dog skull in microstructures. Key words: dog skull, gamma, sterilize


1993 ◽  
Vol 27 (3-4) ◽  
pp. 1-13 ◽  
Author(s):  
Arie H. Havelaar ◽  
Siem H. Heisterkamp ◽  
Janneke A. Hoekstra ◽  
Kirsten A. Mooijman

The general concept of measurement errors is applied to quantitative bacteriological counts on membrane filters or agar plates. The systematic errors of these methods are related to the growth characteristics of the medium (recovery of target organisms and inhibition of non-target organisms) and to its differential characteristics (sensitivity and specificity). Factors that influence the precision of microbiological counts are the variation between replicates, within samples, between operators and between laboratories. It is also affected by the linearity of the method, the verification rate and, where applicable, the number of colonies subcultured for verification. Repeatability (r) and reproducibility (R) values can be calculated on the logarithmic scale.


1939 ◽  
Vol 31 (4) ◽  
pp. 707-713 ◽  
Author(s):  
James E. Weiss ◽  
Charles A. Hunter

1983 ◽  
Vol 17 (4) ◽  
pp. 285-289 ◽  
Author(s):  
R. Boot ◽  
J. Oosterom ◽  
H. C. Walvoort

In the course of post-mortem bacteriological examination of conventional guineapigs, 88 isolates belonging to the Pasteurella-Actinobacillus-group were recovered from 69 of 279 animals (25%). Most isolates were recovered from pneumonic lung, enteritic jejunum and inflamed mammary gland. No relationship was found between biotype and source of isolation. About 50% of isolates were recovered in pure culture or as the predominant micro-organism. It is concluded that members of the Pasteurella-Actinobacillus-group must be considered potentially pathogenic for guineapigs.


1907 ◽  
Vol 7 (1) ◽  
pp. 22-31 ◽  
Author(s):  
R. Tanner Hewlett ◽  
George S. Barton

In view of the importance of a pure milk supply, we considered that it might be of interest to examine chemically, microscopically, and bacteriologically, a number of specimens of milk coming into the Metropolis for which purpose we decided to select samples from the various counties, the milk of which is consigned to London. We found that milk so consigned comes from about twenty-six counties extending from Derby in the North, to Hampshire and Devonshire in the South and South-West, and from Hereford in the West, to Norfolk in the East.


The bacteriological investigation of tetanus, the results of which are summarised in the present communication, was undertaken on behalf of the War Office Committee for the Study of Tetanus. Before the bacteriological examination of a relatively large number of cases of tetanus could be effected, it was found necessary to elaborate a suitable technique, as the existing methods for the cultivation of B. tetani proved to be wholly inadequate.


1921 ◽  
Vol 20 (2) ◽  
pp. 125-131
Author(s):  
K. Freear ◽  
A. T. R. Mattick ◽  
R. Stenhouse Williams

The following paper contains a study of the results of bacteriological examinations of samples of Grade “A” (Certified) milk, some of which were 24 hours old and others 30 hours old at the time of examination. This work was started in 1916 and is still being carried on. Some of the results have already been published. For purposes of comparison, these results, together with further series, are included in the present paper.


1953 ◽  
Vol 51 (2) ◽  
pp. 185-194 ◽  
Author(s):  
L. A. Allen ◽  
J. Grindley ◽  
Eileen Brooks

Chemical and bacteriological examination of muds from sources differing widely in the degree of pollution to which they were subject showed great differences in the contents of carbon, nitrogen and sulphide. These differences were not correlated with differences in the severity of faecal pollution. The amount of organic matter available for growth of micro-organisms in the mud of different depths was not reflected in the figures for organic carbon. A convenient index of this factor was obtained by measuring the volume of gas evolved during anaerobic digestion over a prolonged period of incubation. The rate of evolution was increased by the addition of an inoculum of digested sludge from a sewage works.Sulphate-reducing bacteria appeared to be of two different types. In samples of mud from fresh-water lakes much higher counts were usually obtained in a medium containing comparatively low concentrations of inorganic salts and of lactate than in a medium containing much higher concentrations of these constituents. In samples from locations where conditions were more saline the reverse was usually true.Counts of Bact. coli and of Strep, faecalis together probably constitute the best index of faecal pollution in the examination of samples of mud. These organisms are, however, largely confined to the surface layers.


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