THE EFFECT OF GAMMA STERILIZATION AND ITS EFFECT ON MICROSCOPIC STRUCTURE OF DEEP FROZEN DOG SKULL

2018 ◽  
pp. 37-40
Author(s):  
Phuong Thao Tien Nguyen ◽  
Anh Hung Tran ◽  
Van Tam Le ◽  
Huu Thao Phung ◽  
Phan Quynh Anh Nguyen
Keyword(s):  
Structural Change ◽  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Key Words ◽  
Bone Matrix ◽  
Infected Tissue ◽  
Microscopic Structure ◽  
Bacteriological Examination ◽  
Microscopic Scale

Objective: To evaluate the effect of gamma sterilization and its effect on microscopic structure of deeply frozen dog skull. Subjects and methods: 50 pairs of dog skull fragments have the same size,which were taken symmetrically through the midline of the skull, were divided into two lots. Evident lot consisting of 50 pieces are just deeply frozen, not irradicated. Experimental lot which was composed of 50 pieces were deeply frozen and exposed to 25kGy of gamma radiation. Afterwards, to evaluate the effect of gamma sterilization and compare the alter of bone matrix between lot groups. Results: After gamma irradiation, all dog skull fragments were negative with bacteriological examination. In microscopic scale, no difference was found between the two lots and there was no structural change in the irradiated fragments of all dog skull. Conclusion: Gamma irradiation with 25 kGy: (i) It has the ability to kill bacteria completely in the type of bacteria commonly infected tissue. (ii) It does not alter the structure of dog skull in microstructures. Key words: dog skull, gamma, sterilize

FIXATION AND IMMOBILIZATION OF RECENTLY ADDED IN SELECTED ONTARIO AND QUEBEC SOILS

1989 ◽  
Vol 69 (2) ◽  
pp. 391-400 ◽  
Author(s):  
C. F. DRURY ◽  
E. G. BEAUCHAMP ◽  
L. J. EVANS
Keyword(s):  
Biological Activity ◽  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Key Words ◽  
High Recovery ◽  
Air Drying ◽  
Ammonium Fixation

Low recovery of recently added [Formula: see text] fertilizer as [Formula: see text] was observed in a Bainsville (Orthic Humic Gleysol) and a Dalhousie (Gleyed Eutric Brunisol) soil, whereas a Brookston (Orthic Humic Gleysol) and a Conestogo (Gleyed Melanic Brunisol) soil had a high recovery. The former two soils had higher vermiculite contents than the latter two soils. Therefore, [Formula: see text] fixation was believed to be responsible for the low recovery. Between 18 and 23% of added [Formula: see text] was fixed after 15 d in the Bainsville and Dalhousie soils whereas only 1–3% of added [Formula: see text] was fixed in the Brookston and Conestogo soils. Potassium pre-addition (with/without air drying) increased the apparent recovery in the Bainsville and Dalhousie soils but did not affect the actual recovery. Potassium pre-addition did not block subsequent [Formula: see text] fixation, rather it resulted in a release of native 14N. Gamma irradiation was used to inhibit biological activity. Between 1 and 7% of added 15N was immobilized in these soils after 15 d. Key words: Ammonium fixation, immobilization, vermiculites, 15N gamma radiation, potassium, native N, ammonium exchange

Mass Treatment of Flooded Archival Materials by Gamma Radiation

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Matteo Montanari ◽  
Sara Pipponzi ◽  
Pietro Livi ◽  
Antonio Prodi
Keyword(s):  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Culture Method ◽  
Test Species ◽  
Atp Assay ◽  
Recovery Processes ◽  
Mycelia Growth ◽  
Thermal Drying ◽  
Radiation Treatments ◽  
Main Components

Abstract This work describes mass recovery processes of flooded archival materials at industrial scale. The presence of fungi on paper represents a threat to the integrity of the document because they degrade cellulose, one of the main components of paper. Gamma radiation treatments are investigated as mass disinfection agents for their high penetrating power, speed of treatment, and absence of risk due to chemical residuals. We compared two different recovery processes: thermal drying followed by gamma irradiation and gamma irradiation followed by thermal drying. Both these processes were conducted simultaneously on naturally contaminated archival items and on paper specimens artificially contaminated with species test. Efficacy was assessed by culture method and ATP assay, right after the treatments and after four years of storage at room temperature. Coupling gamma irradiation with a drying step with dry heat at 55–60 °C reduces the fungal loads on natural items up to levels close to the detection limits, and the reduction is maintained after four years. On artificial specimens, spore germination is completely inhibited, mycelia growth is also highly affected, but the melanised test species appear to be more resistant. A synergistic effect between gamma irradiation, water content, and thermal drying is highlighted in this paper.

scholarly journals Effect of gamma irradiation on fatty acid composition of total lipids in rabbit skin

2014 ◽  
Vol 68 (3) ◽  
pp. 34-36
Author(s):  
S. Kostyuk ◽  
A. Busenko
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Acid Composition ◽  
Total Lipids ◽  
Rabbit Skin

It is found that gamma radiation leads to a significant decrease in the skin of rabbits content of these fatty acids, as meristinіс, pantadekanovaya, palmitic, palmitoleic, linoleic, arachidonic, and at the end issledvany, ie 76 Tide day, the concentration of fatty acids increased, and meristinіс palmitoleic and close to the physiological norm.

Histopathology of Albugo tragopogonis on stems and petioles of sunflower

1999 ◽  
Vol 77 (1) ◽  
pp. 175-178
Author(s):  
H Krüger ◽  
A Viljoen ◽  
P S Van Wyk
Keyword(s):  
Cell Division ◽  
Key Words ◽  
Helianthus Annuus ◽  
Systemic Infection ◽  
Infected Tissue ◽  
Tissue Integrity ◽  
Callose Formation ◽  
Stem Lesions ◽  
Lignin Deposition

Stem lesions in sunflower caused by Albugo tragopogonis (Pers.) S.F. Gray developed individually from primary infections and did not result from a systemic infection. Cell division and callose formation were not observed, but weak lignin deposition occurred in infected tissues. Hyphae occurred intercellularly in stems in the cortex, cambium, vascular rays, and pith. In petioles parenchymatous tissue was heavily colonized in contrast to lightly colonized collenchymatous hypodermis. The middle lamellae of cells in infected tissue were dissolved, and cells degenerated and eventually collapsed. Stem infections lead to deterioration of tissue integrity, weakening of stems, and finally to lodging of stems (breaking over).Key words: Albugo tragopogonis, Helianthus annuus, histopathology, stem lodging.

Presente e futuro della cultura contadina

2009 ◽  
pp. 206-214
Author(s):  
Osvaldo Pieroni
Keyword(s):  
Structural Change ◽  
Key Words ◽  
Family Farm ◽  
Farm Land ◽  
Scientific Level ◽  
Specific Relationship ◽  
The Family ◽  
Classical Image ◽  
Definition Of ◽  
Peasant Culture

- While a re-evaluation of the peasant role is emerging at the scientific level, public and institutional opinion is still influenced by the classical image of antimodern peasants. In the last years, rural sociologists and some agrarian economists have emphasized the persistence of the "peasant model of farming". Considering the present food and environmental crises, the new functions developed in the family farm represent a structural change, both in social and economic terms. By proposing the definition of strategic fertility as a specific relationship with the soil in view of a durable reproduction, the author is outlining the new relevant aspects of the peasant culture. Key words: peasant culture; peasant agriculture; family farm; land; biological fertility; co-evolution.

scholarly journals Gamma Radiation-Induced Disruption of Cellular Junctions in HUVECs Is Mediated through Affecting MAPK/NF-κB Inflammatory Pathways

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
H. Wang ◽  
R. Chandra Segaran ◽  
L. Y. Chan ◽  
Aref A. M. Aladresi ◽  
A. Chinnathambi ◽  
...  
Keyword(s):  
Gamma Radiation ◽  
Cell Viability ◽  
Gamma Irradiation ◽  
Mapk Pathway ◽  
Umbilical Vein ◽  
Mitogen Activated Protein Kinase ◽  
Migration Ability ◽  
Protein Levels ◽  
Cellular Junctions ◽  
Radiation Induced

Ionizing radiation-induced cardiovascular diseases (CVDs) have been well documented. However, the mechanisms of CVD genesis are still not fully understood. In this study, human umbilical vein endothelial cells (HUVECs) were exposed to gamma irradiation at different doses ranging from 0.2 Gy to 5 Gy. Cell viability, migration ability, permeability, oxidative and nitrosative stresses, inflammation, and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) pathway activation were evaluated postirradiation. It was found that gamma irradiation at doses ranging from 0.5 Gy to 5 Gy inhibited the migration ability of HUVECs without any significant effects on cell viability at 6 h and 24 h postirradiation. The decreased transendothelial electrical resistance (TEER), increased permeability, and disruption of cellular junctions were observed in HUVECs after gamma irradiation accompanied by the lower levels of junction-related proteins such as ZO-1, occludin, vascular endothelial- (VE-) cadherin, and connexin 40. The enhanced oxidative and nitrosative stresses, e.g., ROS and NO2- levels and inflammatory cytokines IL-6 and TNF-α were demonstrated in HUVECs after gamma irradiation. Western blot results showed that protein levels of mitogen-activated protein kinase (MAPK) pathway molecules p38, p53, p21, and p27 increased after gamma irradiation, which further induced the activation of the NF-κB pathway. BAY 11-7085, an inhibitor of NF-κB activation, was demonstrated to partially block the effects of gamma radiation in HUVECs examined by TEER and FITC-dextran permeability assay. We therefore concluded that the gamma irradiation-induced disruption of cellular junctions in HUVECs was through the inflammatory MAPK/NF-κB signaling pathway.

Degradation and Anticarboxylesterase Activity of Disulfoton and Phorate after 60Co Gamma Irradiation

1969 ◽  
Vol 52 (4) ◽  
pp. 805-811
Author(s):  
D L Grant ◽  
C R Sherwood ◽  
K A Mccully
Keyword(s):  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Low Temperatures ◽  
Brilliant Green ◽  
Organophosphorus Pesticides ◽  
Beef Liver ◽  
Oxygen Analog ◽  
60Co Gamma ◽  
Dose Levels

Abstract Disulfoton and phorate were subjected to gamma radiation from 60Co at dose levels of 0, 0.1, 0.5, 1, 2, and 4 Mrad. The inhibition of beef liver carboxylesterases by disulfoton and phorate in vitro was greater with increasing doses of radiation. The degradation of these organophosphorus pesticides was monitored by GLC with electron capture and phosphorus detectors, by TLC with detection by Brilliant green, and by enzyme inhibition with 5-bromoindoxyl acetate as substrate. The pesticides were degraded to the greatest extent in hexane or acetone solution. Less decomposition occurred at low temperatures. The sulfoxide and oxygen analog were present in most irradiated samples. The sulfone, oxygen analog sulfone, and oxygen analog sulfoxide were present in some of the disulfoton and phorate samples which were irradiated with 4 Mrad.

Effect of Gamma Radiation on Dyeing of Cotton Fabric with Reactive Blue 13

2011 ◽  
Vol 15 (3) ◽  
pp. 107-114 ◽  
Author(s):  
Iram Batool ◽  
Ijaz Ahmad Bhatti ◽  
Shahid Adeel ◽  
Muhammad Abbas
Keyword(s):  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Radiation Treatment ◽  
Reactive Dye ◽  
Iso Standard ◽  
Standard Methods ◽  
Colour Strength ◽  
Dyeing Parameters ◽  
Optimized Conditions ◽  
Temperature Time

The effect of gamma radiation on the dyeing of cotton with Reactive Blue 13 dye powder has been investigated. The cotton and dye powder are irradiated to absorb doses of 100, 200, 300, 400 and 500 Gy by using a Co-60 gamma irradiator. Dyeing is performed by using irradiated and un-irradiated cotton with irradiated and un-irradiated dye powder, in order to observe the effect of radiation treatment on the colour strength of the reactive dye on cotton. Different dyeing parameters such as temperature, time and p H are optimized using irradiated cotton and dye powder. The effect of gamma irradiation on the colour strength and Lab values has been observed through optimized conditions of dyeing. The colour strength and Lab values are obtained by spectra flash spectrophotometer (SF650). ISO standard methods are employed to study the effect of gamma radiation on the Colourfastness properties of fabric. It can be demonstrated that gamma irradiation not only improves the colour strength of the dye in irradiated cotton, but also enhances the Colourfastness properties.

The effectiveness of prophylactic sterilisation of beehive materials by gamma radiation

1988 ◽  
Vol 28 (6) ◽  
pp. 809 ◽  
Author(s):  
BP Oldroyd ◽  
C Reinganum
Keyword(s):  
Weight Gain ◽  
Apis Mellifera ◽  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Average Weight ◽  
Nosema Apis ◽  
Average Gain ◽  
The Cost ◽  
Average Weight Gain

Honeybee (Apis mellifera) colonies hived in beehive materials that had been sterilised by gamma irradiation had an average weight gain of 29.3 kg while those housed in untreated hives had an average gain of 27.1 kg.Bees sampled from both the treated and untreated colonies were examined for the presence of Nosema apis and virus, causitive organisms of stress related diseases, at the end of the season. No significant differences were found. The slight increase in weight (2.2 kg) of the sterilised colonies was not sufficient to justify the cost of prophylactic sterilisation of beehive equipment.

Sign in / Sign up

Export Citation Format

Share Document