Silver nitrate and silver-thiosulphate mitigates callus and leaf abscission during Shisham clonal micro-propagation

Silver impregnation techniques have been used for the demonstration of the complex carbohydrates in electron microscopy. However, the silver stains were believed to be technically sensitive and time consumming to perform. Currently, due to the need to more specifically evaluate immune complex for localization in certain renal diseases, a simplified procedure in conjunction with the use of the microwave has been developed and applied to renal and other biopsies. The procedure is as follows:Preparation of silver methenamine solution:1. 15ml graduated, clear polystyrene centrifuge tube (Falcon, No. 2099) was rinsed once with distilled water.2. 3% hexamethylene tetramine (methenamine) was added into the centrifuge tube to the 6ml mark.3. 3% silver nitrate was added slowly to the methenamine to the 7ml mark while agitating. (Solution will instantly turn milky in color and then clear rapidly by mixing. No precipitate should be formed).4. 2% sodium borate was added to the solution to the 8ml mark, mixed and centrifuged before use.

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Development of a Protocol for in vitro Micro Propagation of Celastrus paniculatus Willd. – A Threatened Medicinal Plant

Planta Medica ◽

10.1055/s-0030-1251770 ◽

2010 ◽

Vol 76 (05) ◽

Author(s):

MAN De Silva ◽

WTPSK Senarath ◽

KSS Sugathadasa

Keyword(s):

Medicinal Plant ◽

Threatened Medicinal Plant ◽

Micro Propagation ◽

Celastrus Paniculatus

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Effect of silver nitrate on some mechanical properties of heat polymerizing acrylic resins

Mustansiria Dental Journal ◽

10.32828/mdj.v14i1.768 ◽

2019 ◽

Vol 14 (1) ◽

pp. 110

Author(s):

Assiss. Prof. Dr. Sabiha Mahdi Mahdi ◽

Dr. Firas Abd K. Abd K.

Keyword(s):

Mechanical Properties ◽

Tensile Strength ◽

Silver Nitrate ◽

Surface Hardness ◽

Mechanical Tests ◽

Hardness Tester ◽

Acrylic Resins ◽

The Difference

Aim: The aimed study was to evaluate the influence of silver nitrate on surfacehardness and tensile strength of acrylic resins.Materials and methods: A total of 60 specimens were made from heat polymerizingresins. Two mechanical tests were utilized (surface hardness and tensile strength)and 4 experimental groups according to the concentration of silver nitrate used.The specimens without the use of silver nitrate were considered as control. Fortensile strength, all specimens were subjected to force till fracture. For surfacehardness, the specimens were tested via a durometer hardness tester. Allspecimens data were analyzed via ANOVA and Tukey tests.Results: The addition of silver nitrate to acrylic resins reduced significantly thetensile strength. Statistically, highly significant differences were found among allgroups (P≤0.001). Also, the difference between control and experimental groupswas highly significant (P≤0.001). For surface hardness, the silver nitrate improvedthe surface hardness of acrylics. Highly significant differences were statisticallyobserved between control and 900 ppm group (P≤0.001); and among all groups(P≤0.001)with exception that no significant differences between control and150ppm; and between 150ppm and 900ppm groups(P>0.05).Conclusion: The addition of silver nitrate to acrylics reduced significantly the tensilestrength and improved slightly the surface hardness.

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