Abstract This paper describes the preparation of a chiral stationary phase based on a 2-hydroxypropyl-β-cyclodextrin polymer coated on porous silica. This method requires first the synthesis of a water-soluble cyclodextrin polymer carried out by the polycondensation of 2-hydroxypropyl-β-cyclodextrin with epichlorohydrin under controlled conditions. In the second step, the obtained copolymer was immobilised onto the porous silica surface by physisorption. The macromolecular features of the copolymer were investigated by size exclusion chromatography coupled with a multiple angle laser-light scattering detector. The evolution of the structural parameters of the modified silica beads (specific surface area and pore size distribution) was determined using a nitrogen adsorption method. The enantio-separation capability of the coated silica phase was evaluated for different polycyclic molecules. It was found that the enantioselectivity was strongly influenced by the geometry and shape of the solutes under study and the presence of an acidic modifier in the mobile phase.
Allyltrimethoxysilane as the Reagent of Double Bond Introduction to Porous Silica for Preparation of a Chiral Stationary Phase in Comparison with Allyltriethoxysilane