Quality Assessment of Curcuma longa L. by Gas Chromatography-Mass Spectrometry Fingerprint, Principle Components Analysis and Hierarchical Clustering Analysis

Adulteration has gained much concern in the oils and fats industry due to health and religious issues. This study assesses the effect of heating on the profiling of lard (15% and 30% lard) spiked in refined bleached deodorized (RBD) palm oil at 120 oC, 180 oC and 240 oC for 1, 2 and 3 hours. The volatile compounds released were identified using (gas chromatography mass spectrometry headspace) GC-MS-HS method. Multivariate data from GC-MS-HS were mean centred prior to Principal Components Analysis (PCA) using Unscrambler software. The result obtained from the scores plot for 30% lard and 15% lard in the RBD palm oil showed the same pattern for heating temperature at 120 oC and 180 oC. At 240 oC, both sample and control were scattered in the scores plot. However, the GC-MS-HS technique did not differentiate between 0% lard with 15% or 30% lard in RBD palm oil.

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Clustering analysis of different hop varieties according to their essential oil composition measured by GC/MS

Chemical Papers ◽

10.1515/chempap-2016-0092 ◽

2016 ◽

Vol 70 (12) ◽

Cited By ~ 5

Author(s):

Paulius Kaškonas ◽

Žydrūnas Stanius ◽

Vilma Kaškonienė ◽

Kȩestutis Obelevičius ◽

Ona Ragažinskienė ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Essential Oil ◽

Essential Oils ◽

Volatile Compounds ◽

Clustering Analysis ◽

Essential Oil Composition ◽

Gas Chromatography Mass Spectrometry ◽

Oil Composition ◽

Chromatography Mass Spectrometry

AbstractThis study describes the analysis of total hops essential oils from 18 cultivated varieties of hops, five of which were bred in Lithuania, and 7 wild hop forms using gas chromatography-mass spectrometry. The study sought to organise the samples of hops into clusters, according to 72 semi-volatile compounds, by applying a well-known method,

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Analyses for progesterone in serum by gas chromatography/mass spectrometry: target data for external quality assessment of routine assays.

Clinical Chemistry ◽

10.1093/clinchem/30.10.1696 ◽

1984 ◽

Vol 30 (10) ◽

pp. 1696-1700 ◽

Cited By ~ 15

Author(s):

S J Gaskell ◽

B G Brownsey ◽

G V Groom

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Quality Assessment ◽

External Quality Assessment ◽

Accurate Determination ◽

Gas Chromatography Mass Spectrometry ◽

External Quality ◽

External Quality Assessment Scheme ◽

Chromatography Mass Spectrometry ◽

Target Data

Abstract We describe a procedure for measuring progesterone in plasma and serum by isotope dilution and mass spectrometry. Extraction with use of a microcellulose-coupled antiserum is followed by conversion to the 3-enol heptafluorobutyrate and analysis by gas chromatography/mass spectrometry (GC/MS) with selected ion monitoring, at a resolution of 5000. Interassay CVs were 1.5 to 5.4% for the concentration range 13 to 43 nmol/L. Analyses of various serum volumes showed excellent linearity. Accurate determination of progesterone added to serum was demonstrated. Plasma and serum pools were analyzed to provide target data for use in the U.K. national external quality-assessment scheme for progesterone assays. Direct, non-extraction radioimmunoassays and those incorporating solvent extraction both showed a positive bias with respect to data obtained by the present procedure, but the bias was more marked with the direct assays.

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External quality assessment of assays for cortisol in plasma: use of target data obtained by gas chromatography/mass spectrometry.

Clinical Chemistry ◽

10.1093/clinchem/29.5.862 ◽

1983 ◽

Vol 29 (5) ◽

pp. 862-867 ◽

Cited By ~ 20

Author(s):

S J Gaskell ◽

C J Collins ◽

G C Thorne ◽

G V Groom

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Solvent Extraction ◽

Quality Assessment ◽

High Resolution Mass Spectrometry ◽

External Quality Assessment ◽

Gas Chromatography Mass Spectrometry ◽

Good Precision ◽

External Quality ◽

Target Data

Abstract We describe procedures for measuring cortisol in plasma and serum by isotope dilution and mass spectrometry. A method that incorporated solvent extraction, derivatization, and gas chromatography/high-resolution mass spectrometry provided data of good precision; interassay CVs were generally 3 to 4% for the concentration range of 100-650 nmol/L. Replacing solvent extraction with extraction on a column of Lipidex 1000 or extraction by immunoadsorption yielded data in excellent agreement with the first method. Plasma and serum pools were analyzed to provide target data for use in the U.K. National External Quality Assessment Scheme for cortisol assays. Routine laboratory assays, as judged by comparison with mass-spectrometric data, were generally positively biased except for analysis of a charcoal-stripped plasma supplemented with cortisol. The results emphasize the importance of using unadulterated plasma or serum pools in assessments of steroid assay procedures.

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