Topical Application of Eupatilin Ameliorates Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

Background: Langerhans cells (LCs) polarize the immune milieu towards a T helper type (Th) 1 or Th2 immune response. We investigated the effects of selected tetracyclines on Th cells development mediated by LCs, and their implications for the treatment of atopic dermatitis (AD). Methods: Mice were primed with ovalbumin (OVA) peptide-pulsed LCs, which had been treated with each antibiotic, via the hind footpad. After 5 days, the Th1/Th2 cytokine response in the popliteal lymph nodes was investigated by enzyme-linked immunosorbent assay. The expression of cell surface molecules on LCs was investigated using reverse transcriptase polymerase chain reaction. The therapeutic effects of a selected antibiotic on AD-like skin lesions of NC/Nga mice were assessed in terms of the skin severity score, histological changes in the lesioned skin, the serum level of total IgE, and expression of Th1/Th2 cytokines in lymph nodes and skin lesions. Results: Antibiotic-treated, OVA peptide-pulsed LCs inhibited development of Th2 cells but not Th1 cells. This was accompanied by suppression of T-cell immunoglobulin and mucin domain-containing protein (TIM)-4 expression in LCs. Doxycycline had the greatest activity against Staphylococcus aureus strains isolated from skin lesions of patients with AD, and a strong inhibitory effect on Th2 cell development. Doxycycline suppressed the increase in the skin severity score during the acute phase in NC/Nga mice similar to betamethasone. This suppressive effect was associated with a decrease in the serum IgE level and production of Th2 cytokines in auricular lymph node cells and skin lesions. Conclusion: Topical application of doxycycline to AD lesions would act on both superficial S. aureus colonization and epidermal LCs, thus possibly inhibiting the development of Th2 cells in vivo, with benefits for control of acute inflammation in AD.

Download Full-text

Topical Application of Herbal Mixture Extract Inhibits Ovalbumin- or 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/545497 ◽

2012 ◽

Vol 2012 ◽

pp. 1-9 ◽

Cited By ~ 11

Author(s):

Soon Re Kim ◽

Han-Seok Choi ◽

Hye Sook Seo ◽

Youn Kyung Choi ◽

Yong Cheol Shin ◽

...

Keyword(s):

Atopic Dermatitis ◽

Topical Application ◽

Immunoglobulin E ◽

Elevated Serum ◽

Ethanol Extract ◽

Inflammatory Cells ◽

Skin Lesions ◽

Serum Ige ◽

Beneficial Effects ◽

Blood Eosinophils

KM110329 is four traditional herbal medicine mixtures with anti-inflammatory properties. Atopic dermatitis (AD) is an inflammatory skin disease associated with enhanced T-helper2 (Th2) lymphocyte response to allergens that results in elevated serum eosinophil and Immunoglobulin E (IgE) levels and leukocyte infiltration in atopic skin sites. In this study, we investigated the effect of topical application of KM110329 ethanol extract on the ovalbumin (OVA) or 2,4-dinitrochlorobenzene- (DNCB-) induced AD mouse models. For that purpose, we observed the effects of KM110329 on blood eosinophils, skin mast cells, production of serum IgE, and expression of cytokine mRNA in the atopic dermatitis skin lesions of OVA allergen- or DNCB-treated BALB/c mice. KM110329 significantly reduced blood eosinophils cell numbers in OVA or DNCB-treated BALB/c mice. Histological analyses demonstrated decreased mast cell count as well as dermal infiltration by inflammatory cells. In the skin lesions, mRNA expression of interleukine (IL)-4, IL-13, and IL-17 was inhibited by KM110329. KM110329 also suppressed the production of serum IgE level in both the OVA- and DNCB-induced atopic dermatitis model. Taken together, our results showed that topical application of KM110329 extracts exerts beneficial effects in AD symptoms, suggesting that KM110329 might be a useful candidate for the treatment of AD.

Download Full-text

Topical Application of Josamycin Inhibits Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

Journal of Pharmacy & Pharmaceutical Sciences ◽

10.18433/j3gw3d ◽

2017 ◽

Vol 20 ◽

pp. 38 ◽

Cited By ~ 1

Author(s):

Katsuhiko Matsui ◽

Kanta Tachioka ◽

Kei Onodera ◽

Reiko Ikeda

Keyword(s):

Atopic Dermatitis ◽

Topical Application ◽

Serum Levels ◽

Skin Lesions ◽

Th2 Cells ◽

Therapeutic Effects ◽

Severity Scores ◽

Superficial Skin ◽

Ifn Γ ◽

Th1 And Th2

Background: Patients with atopic dermatitis (AD) have superficial skin colonization by Staphylococcus aureus and an increased number of T helper type 2 (Th2) cells in their peripheral blood. Our previous study showed that josamycin, a macrolide antibiotic, had excellent bactericidal activity against S. aureus strains isolated from AD patients and simultaneously inhibited Th1 and Th2 cell development mediated by Langerhans cells. The purpose of the present study was to evaluate the effect of topical application of josamycin on AD-like skin lesions in NC/Nga mice. Methods: Josamycin (0.1%) was topically administered to NC/Nga mice with AD-like skin lesions induced by 2, 4, 6-trinitrochlorobenzene (TNCB). The therapeutic effects of josamycin were assessed by measurement of the skin severity scores, histological changes in the lesioned skin, serum levels of total IgE, and expression of interferon (IFN)-γ and interleukin (IL)-4 in lymph nodes and skin lesions. Results: Topical treatment with josamycin significantly suppressed the increase in the skin severity score in NC/Nga mice. This suppressive effect was equal to that of betamethasone, and was associated with a decrease in the density of cellular infiltration into the dermis, the mast cell count in the dermis and the serum IgE level. Furthermore, topical application of josamycin reduced the expression of IFN-γ and IL-4 in auricular lymph node cells and the skin lesions. Conclusion: The present results show that topical application of josamycin inhibits the development of AD-like skin lesions in NC/Nga mice. This suggests that topical application of josamycin to AD lesions colonized by S. aureus would be beneficial for control of AD by acting on superficially located S. aureus and by inhibiting the development of Th1 and Th2 cells.This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

Download Full-text

Topical Application of 7,3′,4′-Trihydroxyisoflavone Alleviates Atopic Dermatitis-Like Symptoms in NC/Nga Mice

Planta Medica ◽

10.1055/a-1068-7983 ◽

2019 ◽

Vol 86 (03) ◽

pp. 190-197

Author(s):

Sang Hun Park ◽

Chang Hyung Lee ◽

Ji Yun Lee ◽

Hee Yang ◽

Jong Hun Kim ◽

...

Keyword(s):

Atopic Dermatitis ◽

Topical Application ◽

Histological Analysis ◽

Skin Lesions ◽

Ortho Position ◽

Food Material ◽

Inflammatory Lesions ◽

Tnf Α ◽

Ifn Γ ◽

Phenol Ring

AbstractAtopic dermatitis is a skin disease characterized by chronic inflammatory lesions, and new therapies are needed to address its rising prevalence. Soy isoflavone has been highlighted as a potential new cosmeceutical material that may have applications in atopic dermatitis care. We have developed a technique to attach an additional -OH group to the ortho position of -OH in the phenol ring using a special enzyme. By adding the -OH group to daidzein, 7,3′,4′-trihydroxyisoflavone can be generated for possible use as a cosmeceutical and functional food material. In this study, we sought to examine the anti-atopic effects of 7,3′,4′-trihydroxyisoflavone, an analog of daidzein. Topical application of 7,3′,4′-trihydroxyisoflavone reduced Dermatophagoides farina extract-induced atopic dermatitis symptoms in NC/Nga mice. Histological analysis demonstrated that 7,3′,4′-trihydroxyisoflavone suppressed D. farina extract-induced infiltration of eosinophils and mast cells into skin lesions. We also found that 7,3′,4′-trihydroxyisoflavone significantly reduces the D. farina extract-induced increases in serum IgE and macrophage-derived chemokine (CCL22) levels. We observed that 7,3′,4′-trihydroxyisoflavone suppresses atopic markers including macrophage-derived chemokine (CCL22) and thymus and activation-regulated chemokine (CCL17) in HaCaT cells. 7,3′,4′-Trihydroxyisoflavone also reduced TNF-α/IFN-γ-induced phosphorylation of ERK1/2 and JNK1/2. These results highlight several desirable properties of 7,3′,4′-trihydroxyisoflavone, which support its use as a cosmeceutical ingredient for the treatment of atopic dermatitis.

Download Full-text

Topical Application of Rosa multiflora Root Extract Improves Atopic Dermatitis-Like Skin Lesions Induced by Mite Antigen in NC/Nga Mice

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.b13-00619 ◽

2014 ◽

Vol 37 (1) ◽

pp. 178-183 ◽

Cited By ~ 6

Author(s):

Kwan Hee Park ◽

Mi Sook Jeong ◽

Kwang Jun Park ◽

Young Wook Choi ◽

Seong Jun Seo ◽

...

Keyword(s):

Atopic Dermatitis ◽

Topical Application ◽

Skin Lesions ◽

Root Extract ◽

Rosa Multiflora

Download Full-text

Atopic Dermatitis-Like Skin Lesions Induced by Topical Application of Mite Antigens in NC/Nga Mice

International Archives of Allergy and Immunology ◽

10.1159/000049520 ◽

2001 ◽

Vol 126 (3) ◽

pp. 239-247 ◽

Cited By ~ 58

Author(s):

Tatsuya Sasakawa ◽

Yasuyuki Higashi ◽

Syozo Sakuma ◽

Yoshitaka Hirayama ◽

Yuka Sasakawa ◽

...

Keyword(s):

Atopic Dermatitis ◽

Topical Application ◽

Skin Lesions

Download Full-text

Topical application of a novel ceramide derivative, K6PC-9, inhibits dust mite extract-induced atopic dermatitis-like skin lesions in NC/Nga mice

International Immunopharmacology ◽

10.1016/j.intimp.2007.08.009 ◽

2007 ◽

Vol 7 (13) ◽

pp. 1589-1597 ◽

Cited By ~ 16

Author(s):

Jong Soon Kang ◽

Jong-Kyung Youm ◽

Se Kyoo Jeong ◽

Byeong Deog Park ◽

Won Kee Yoon ◽

...

Keyword(s):

Atopic Dermatitis ◽

Topical Application ◽

Skin Lesions ◽

Mite Extract ◽

Dust Mite

Download Full-text

Topical Application ofChrysanthemum indicum L.Attenuates the Development of Atopic Dermatitis-Like Skin Lesions by Suppressing Serum IgE Levels, IFN-γ, and IL-4 in Nc/Nga Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/821967 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 15

Author(s):

Sunmin Park ◽

Jung Bok Lee ◽

Suna Kang

Keyword(s):

Atopic Dermatitis ◽

Topical Application ◽

Immunoglobulin E ◽

Clinical Symptoms ◽

Secondary Infection ◽

Skin Lesions ◽

Mrna Levels ◽

Dorsal Skin ◽

Gene Expressions ◽

Serum Ige

Chrysanthemum indicum L. (CIL) is widely used as an anti-inflammatory agent in Asia and our preliminary study revealed that CIL reduced interleukin (IL)-4 and IL-13 in 2,4-dinitrochlorobenzene (DNCB)-treated HaCaT cells, a human keratinocyte cell line. We investigated the atopic dermatitis (AD) effect of topically applied CIL in mice with AD-like symptoms. After topical application of 1,3-butylen glycol (control), CIL-Low (5%), CIL-High (30%), or 0.1% hydrocortisone (HC) on the AD-like skin lesions in DNCB-treated NC/Nga mice for 5 weeks, the ear thickness, mast cell infiltration, and serum immunoglobulin E (IgE), IgG1, IL-4 and interferon (IFN)-γwere measured. The gene expressions of IL-4, IL-13, and IFN-γin the dorsal skin were assayed. CIL treatment dosedependently reduced severity of clinical symptoms of dorsal skin, ear thickness, and the number of mast cells and eosinophils. CIL-High significantly decreased serum IgE, IgG1, IL-4, and IFN-γlevels and reduced mRNA levels of IFN-γ, IL-4, and IL-13 in dorsal skin lesion. The improvement by CIL-High was similar to HC, but without its adverse effects such as skin atrophy maceration, and secondary infection. In conclusion, CIL may be an effective alternative substance for the management of AD.

Download Full-text