Crucial role of stimulator of interferon genes-dependent signaling in house dust mite extract-induced IgE production

Stimulator of interferon genes (STING) is a DNA sensor that responds to pathogens and induces type I interferon production. Herein, the role of STING in house dust mite extract (HDM)-induced allergic asthma was investigated. C57BL/6 wild-type (WT) and Sting−/− mice were intratracheally sensitized with HDM, and the bronchoalveolar lavage fluid (BALF), sera, lungs, and mediastinal lymph nodes (MLNs) were analyzed. The total and HDM-specific serum IgE levels were lower in Sting−/− mice than in WT mice. B cell and IgE-positive B cell proportion in BALF and MLNs, respectively, was significantly lower in Sting−/− mice than in WT mice. Additionally, cyclic GMP-AMP, a STING ligand, augmented total and HDM-specific serum IgE levels and B cell proportion in BALF when applied in combination with HDM. To elucidate the role of STING in IgE production, follicular helper T (Tfh) cells, which are involved in B cell maturation, were investigated. Tfh cell proportion in MLNs decreased in Sting−/− mice, and IL-4 and IL-13 production by HDM-restimulated MLN cells from HDM-sensitized mice was decreased in Sting−/− mice compared with WT mice. Thus, STING plays an important role in the maturation and class switching of IgE-producing B cells in allergic inflammation via Tfh cells.

Comparison of Therapeutic Efficacy of Spa Mud and Bath Applications in the Treatment of Experimentally Induced Psoriasis and Eczema in Rats

Psoriasis and eczema are most common diseses of human and seen some animals. In this research project, a total of 49 Albino rats, 25 of which were psoriasis and 24 of eczema, were used. The back of all the animals were shaved 3 cm x 2 cm in size, and were deepilated using a 50% barium sulfate solution and deepillation tape. Then, 5% imiquimod was applied to the area for 5-6 days in the rats in psoriasis group, whereas 2%, 4-dinitrochlorobenzene was performed 2 times per day for one week in eczema group animals along with 150 μg of mite extract (Dermatophagoides farinae). After diseases formation in both groups, animals were divided into control and study groups, and then treatment period was started. In the rats with psoriasis and eczema, clinical, hematological, blood biochemical and histopathological findings were determined. In the light of the data obtained; it was concluded that a 21-day spa treatment in rats with psoriasis and eczema was very successful and could be considered as an option in the treatment of these diseases or it would increase the success of the treatment when combinated with classical medical treatments.

Safety and effectiveness of a microcrystalline tyrosine-associated mite extract immunotherapy for allergic rhinitis

Aim: To assess the safety and effectiveness of an allergen immunotherapy (AIT) with a microcrystalline tyrosine-associated mite allergoid in real-world patients with allergic rhinitis (AR). Materials & methods: Retrospective, multicenter study assessing the safety of AIT in patients aged 5 to 65 years with AR, with or without asthma, sensitized to mites. Secondary objective was effectiveness, measured as unscheduled visits to healthcare centers and emergency rooms, rhinitis and asthma evolution, medication use and patients’ and physicians’ disease perception 12 months before and after treatment. Results: The 306 patients evaluated, with a mean (standard deviation) age of 29.68 (14.66) years, received different treatment compositions and regimens, and 25 (8.2%) experienced nonserious adverse reactions. Unscheduled visits to the specialist and emergency room admissions significantly decreased after immunotherapy (mean [standard deviation] 2.11 [1.95] and 0.3 [0.93] vs 0.66 [1.09] and 0.02 [0.2], before and after treatment, respectively). Rhinitis and asthma classification (‘AR and its impact on asthma’ and ‘Guía Española para el Manejo del Asma’, respectively) significantly changed (p < 0.0001 for all classifications), showing symptom reduction after AIT. Median (interquartile range)-combined rhinitis and combined asthma medication scores significantly decreased (4.0 [1.33, 7.0] vs 0.25 [0, 10.0]; p < 0.0001 and 6.94 [1.5, 6.0] vs 0.67 [0, 4.67]; p < 0.0001) within 12 months before and after starting AIT, respectively. Conclusion: AIT with microcrystalline tyrosine-associated mite allergoid appears to be safe and effective in treating rhinitis caused by mites.

Utilization of Human Skin Equivalent in Research of Scabies Pathogenesis

Scabies is a tropical disease that requires large-scale control. Research on the pathogenesis of scabies in Indonesia is still rare. Meanwhile, in tropical countries, studies related to the course of scabies have been done quite a lot, one of which is by using a human skin model. The purpose of writing this article is to discuss the use of human skin modeling technology in researching the course of scabies. Research using HSE demonstrated modulation of cytokine secretion, expression of cell adhesion molecules, and gene expression in response to the scabies mite extract molecule.