Cutaneous leishmaniasis (CL) is vector-borne disease, and endemic in most regions of Iraq specially with poor populations. Natural resistance associated macrophage protein 1 (NRAMP1) gene play a essential role in susceptibility to CL and disease pathology, NRAMP1 influences aproduction and activation of pro-inflammatory cytokines (TNF-α and IL-1β). Pro- and anti-inflammatory cytokines play a essential role in susceptibility/ resistance and the immunopathogenesis of Leishmania infection, these cytokines are crucial factors in the initiationand enhances of protective immunity against Leishmania infection, this study aimed to studding effect of polymorphism in NRAMP1 genes on cytokines secretion, and their effect in susceptibility to CL infection. Samples of blood were collected from (60) patients with CL and (32) apparently healthy controls. Polymorphism of NRAMP1 (D543N) detected in patients and control groups by PCR-RFLP technique. While (TNF-α and IL-1β) cytokine concentration detected by ELIS technique using a quantitative sandwich enzyme immunoassay technique.indicate to effect of NRAMP1 Gene Polymorphism on levels of(TNF-α and IL-1β) cytokines and this a clearly recorded in present study were A allele is associated with lower levels of(TNF-α and IL-1β)in patients and control groups compression to that absorbed in allele Gwith statically significant (P ≤ 0.05).
NRAMP1 Gene Polymorphism and Susceptibility to Cutaneous Leishmaniasis in Iraq