Effect of NRAMP1 Gene Polymorphism on levels of(TNF-α and IL-1β) cytokinesin Cutaneous Leishmaniasis patients in Iraq

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Ghada B Alomashi ◽  
Hasan R Khudhur
Keyword(s):  
Gene Polymorphism ◽  
Cutaneous Leishmaniasis ◽  
Inflammatory Cytokines ◽  
Essential Role ◽  
Natural Resistance ◽  
Leishmania Infection ◽  
Control Groups ◽  
Tnf Α ◽  
Nramp1 Gene ◽  
And Control

Cutaneous leishmaniasis (CL) is vector-borne disease, and endemic in most regions of Iraq specially with poor populations. Natural resistance associated macrophage protein 1 (NRAMP1) gene play a essential role in susceptibility to CL and disease pathology, NRAMP1 influences aproduction and activation of pro-inflammatory cytokines (TNF-α and IL-1β). Pro- and anti-inflammatory cytokines play a essential role in susceptibility/ resistance and the immunopathogenesis of Leishmania infection, these cytokines are crucial factors in the initiationand enhances of protective immunity against Leishmania infection, this study aimed to studding effect of polymorphism in NRAMP1 genes on cytokines secretion, and their effect in susceptibility to CL infection. Samples of blood were collected from (60) patients with CL and (32) apparently healthy controls. Polymorphism of NRAMP1 (D543N) detected in patients and control groups by PCR-RFLP technique. While (TNF-α and IL-1β) cytokine concentration detected by ELIS technique using a quantitative sandwich enzyme immunoassay technique.indicate to effect of NRAMP1 Gene Polymorphism on levels of(TNF-α and IL-1β) cytokines and this a clearly recorded in present study were A allele is associated with lower levels of(TNF-α and IL-1β)in patients and control groups compression to that absorbed in allele Gwith statically significant (P ≤ 0.05).

Association between Vaspin rs2236242 Gene Polymorphism and Psoriasis Vulgaris

2020 ◽  
pp. 1-6
Author(s):  
Nazli Dizen-Namdar ◽  
Raziye Akcilar ◽  
Zeynep Bayat
Keyword(s):  
Gene Polymorphism ◽  
Metabolic Disorders ◽  
Psoriasis Vulgaris ◽  
Turkish Population ◽  
Inflammatory Skin Disease ◽  
Control Groups ◽  
Diagnostic Biomarkers ◽  
Increased Risk ◽  
Visceral Adipose ◽  
And Control

<b><i>Background:</i></b> Psoriasis known as a chronic inflammatory skin disease is accompanied by metabolic disorders such as obesity, diabetes, and dyslipidemia. Vaspin (a serine protease inhibitor derived from visceral adipose tissue) is a newly identified adipokine and a link between inflammation and obesity has been reported. We aimed to determine whether vaspin gene polymorphism is associated with the development and/or clinical features of psoriasis vulgaris. <b><i>Methods:</i></b> Our study group consisted of 96 psoriasis vulgaris patients and 100 matched controls. Vaspin rs2236242 gene was genotyped using PCR. <b><i>Results:</i></b> The vaspin genotypes showed a meaningful difference between psoriasis and control groups (<i>p</i> = 0.02). The frequency of the vaspin rs2236242 TT genotype was lower in psoriasis patients than in control participants (<i>p</i> &#x3c; 0.05). The TA genotype was associated with a 2.38-fold increased risk of psoriasis compared to the TT genotype (<i>p</i> = 0.007, odds ratio: 2.38; 95% confidence interval: 1.25–4.55), but not the AA genotype. All subjects were the Turkish population, the study in other populations is needed and the sample size was small in number. <b><i>Conclusion:</i></b> Our study demonstrated that vaspin rs2236242 polymorphism is related to psoriasis in the Turkish population. Polymorphisms of the vaspin gene might serve as diagnostic biomarkers of psoriasis.

scholarly journals In Vivo Study of the Action of a Topical Anti-Inflammatory Drug In Rat Teeth Submitted To Dental Bleaching

2018 ◽  
Vol 29 (6) ◽  
pp. 555-561 ◽  
Author(s):  
Francine Benetti ◽  
André Luiz Fraga Briso ◽  
Luciana Louzada Ferreira ◽  
Marina Carminatti ◽  
Larissa Álamo ◽  
...  
Keyword(s):  
Statistical Tests ◽  
Control Groups ◽  
Pulp Tissue ◽  
Necrosis Factor Alpha ◽  
Dental Bleaching ◽  
Tnf Α ◽  
Pulp Inflammation ◽  
Significant Difference ◽  
Anti Inflammatory ◽  
And Control

Abstract Bleaching gel containing hydrogen peroxide (H2O2) cause damages in pulp tissue. This study investigated the action of a topical anti-inflammatory, the Otosporin®, in rats’ bleached teeth with the null hypothesis of which the Otosporin® is no able to minimize the pulp inflammation that bleaching gel generates. The rat’s molars were divided into groups: BLE: bleached (35% H2O2 concentration /single application of 30 min); BLE-O: bleached followed by Otosporin® (10 min); and control: placebo gel. In the second day after dental bleaching, the rats were killed, and the jaws were processed for hematoxylin-eosin and immunohistochemistry analysis for tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-17. The data collected were subjected to Kruskal-Wallis and Dunn statistical tests with at a 5% level of significance (p<0.05). The BLE group had moderate to strong inflammation in the occlusal third of the coronary pulp, with necrotic areas; and BLE-O, mild inflammation (p<0.05). There was a significant difference in the occlusal and middle thirds of the coronary pulp between the BLE with BLE-O and control groups (p<0.05). There was no difference in the cervical third (p>0.05). The BLE group had a high immunoexpression of TNF-α than BLE-O and control groups (p<0.05), with moderate and mild immunoexpression, respectively. Regarding IL-6 and IL-17, the BLE group had higher immunoexpression than control (p<0.05); the BLE-O was similar to the control (p>0.05). The topical anti-inflammatory Otosporin® can reduce pulp inflammation after dental bleaching in the rat teeth.

scholarly journals Association between Luteinizing Hormone/Choriogonadotropin Receptor Ins18LQ Gene Polymorphism and Polycystic Ovary Syndrome

2020 ◽  
Vol 8 (A) ◽  
pp. 517-520
Author(s):  
Hilma Putri Lubis ◽  
Muhammad Fidel Ganis Siregar ◽  
Ichwanul Adenin ◽  
Binarwan Halim ◽  
Henry Salim Siregar ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Polycystic Ovary Syndrome ◽  
Gene Polymorphism ◽  
Polycystic Ovary ◽  
Control Group ◽  
Control Groups ◽  
Ovary Syndrome ◽  
Significant Difference ◽  
Pcos Group ◽  
And Control

BACKGROUND: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders of women in the childbearing period. However, its pathophysiology is still unclear. Certain polymorphisms of the luteinizing hormone/choriogonadotropin receptor (LHCGR) genes may lead to changes in the bioactivity of this hormone. The important functional role of LHCGR in the metabolism of androgen and ovulation, the LHCGR gene variant, may be related to the risk of PCOS. AIM: The aim of this study was to evaluate the association between LHCGR Ins18LQ gene polymorphism and PCOS. METHODS: A case–control study was performed in women with PCOS and non-PCOS from May 2019 to October 2019 in HFC IVF Center. We included 50 women with PCOS and 50 healthy controls. Polymorphism of the LHCGR (ins18LQ) gene was genotyped using polymerase chain reaction-restriction fragment length polymorphism. RESULTS: From this study, we found that there was no significant difference in the proportion of ages between the groups (p > 0.05). There were significant differences in the characteristics of body mass index, FSH level, LH level, and LH/FSH ratio between the PCOS and control groups (p < 0.05). We also found that the proportion of heterozygote variant non-ins/ins was higher in the PCOS group compared to the control group, but there was no significant difference between the polymorphisms of the non-ins and non-nonins variants between the PCOS and control groups (p = 0.269). The frequency of ins alleles was higher in the PCOS group compared to the control group. CONCLUSION: There was no significant association between LHCGR ins18LQ gene polymorphism and PCOS.

TNF-α and intPLA2 genes' polymorphism in anorexia nervosa

2004 ◽  
Vol 16 (6) ◽  
pp. 290-294 ◽  
Author(s):  
Agnieszka Slopien ◽  
Filip Rybakowski ◽  
Monika Dmitrzak-Weglarz ◽  
Piotr Czerski ◽  
Joanna Hauser ◽  
...  
Keyword(s):  
Anorexia Nervosa ◽  
Gene Polymorphism ◽  
Control Group ◽  
Study Group ◽  
Intron 1 ◽  
Tnf Α ◽  
The Mean ◽  
Polymerase Chain ◽  
And Control ◽  
Necrosis Factor

Objective:The aim of this study was the assessment of −308G/A tumor necrosis factor (TNF)-α gene polymorphism and intPLA2 gene polymorphism in patients with anorexia nervosa (AN) and healthy controls.Subjects:We studied 91 non-related patients with AN and 144 healthy women (blood donors and students). The mean age of women from study group was 18.22 years (SD ± 3.13 years) and from control group was 31.71 years (SD ± 8.22).Methods:Gene polymorphisms were studied with the use of polymerase chain reaction-restriction fragment length polymorphism method. TNF-α gene polymorphism consists of G/A substitution in −308 promoter region. IntPLA2 gene polymorphism is related to intron 1, in which restrictive region is found and recognized by BanI enzyme.Results:We did not obtain statistically significant differences in the frequency of genotypes and alleles of −308G/A TNF-α polymorphism between the study and control groups (genotypes: P = 0.106, alleles: P = 0.076). We did analogous analysis in the restrictive and bulimic subgroups. We did not observe statistically relevant differences in the frequency of genotypes (P = 0.700) and alleles (P = 0.305). We did not obtain statistically relevant difference in the frequency of genotypes and alleles of intPLA2 gene between the study group and controls (genotypes: P = 0.300, alleles: P = 0.331). We did analogous analysis in both subgroups of AN. We did not observe statistically relevant differences in the frequency of genotypes (P = 0.344) and alleles (P = 0.230).Conclusions:There was no statistically relevant trend for the association between TNF-α polymorphism and AN. We did not find association between studied polymorphism of intPLA2 gene and risk of AN.

scholarly journals Modulation of Adipokines and Cytokines in Gestational Diabetes and Macrosomia

2006 ◽  
Vol 91 (10) ◽  
pp. 4137-4143 ◽  
Author(s):  
J.-M. Atègbo ◽  
O. Grissa ◽  
A. Yessoufou ◽  
A. Hichami ◽  
K. L. Dramane ◽  
...  
Keyword(s):  
Gestational Diabetes ◽  
Inflammatory Cytokines ◽  
Interferon Γ ◽  
Down Regulation ◽  
Tnf Α ◽  
Low Levels ◽  
Th1 Cytokines ◽  
And Control ◽  
Diabetic Mothers

Abstract Context/Objective: Not much is known about the implication of adipokines and different cytokines in gestational diabetes mellitus (GDM) and macrosomia. The purpose of this study was to assess the profile of these hormones and cytokines in macrosomic babies, born to gestational diabetic women. Design/Subjects: A total of 59 women (age, 19–42 yr) suffering from GDM with their macrosomic babies (4.35 ± 0.06 kg) and 60 healthy age-matched pregnant women and their newborns (3.22 ± 0.08 kg) were selected. Methods: Serum adipokines (adiponectin and leptin) were quantified using an obesity-related multiple ELISA microarray kit. The concentrations of serum cytokines were determined by ELISA. Results: Serum adiponectin levels were decreased, whereas the concentrations of leptin, inflammatory cytokines, such as IL-6 and TNF-α, were significantly increased in gestational diabetic mothers compared with control women. The levels of these adipocytokines were diminished in macrosomic babies in comparison with their age-matched control newborns. Serum concentrations of T helper type 1 (Th1) cytokines (IL-2 and interferon-γ) were decreased, whereas IL-10 levels were significantly enhanced in gestational diabetic mothers compared with control women. Macrosomic children exhibited high levels of Th1 cytokines and low levels of IL-10 compared with control infants. Serum IL-4 levels were not altered between gestational diabetic mothers and control mothers or the macrosomic babies and newborn control babies. Conclusions: GDM is linked to the down-regulation of adiponectin along with Th1 cytokines and up-regulation of leptin and inflammatory cytokines. Macrosomia was associated with the up-regulation of Th1 cytokines and the down-regulation of the obesity-related agents (IL-6 and TNF-α, leptin, and adiponectin).

scholarly journals Evaluation of immune cellular enzymes, oxidative and nitrosative Stress in Saudi Patients with Cutaneous Leishmaniasis in Al-AHssa, before and after treatment

2020 ◽  
Author(s):  
Mossad Ahmad Saif ◽  
Hamdan Ibrahim Al-Mohammad
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Reactive Nitrogen Species ◽  
Nitrosative Stress ◽  
Reduced Glutathione ◽  
Oxygen Species ◽  
Control Groups ◽  
Oxidative And Nitrosative Stress ◽  
Before And After ◽  
And Control ◽  
Saudi Patients

Abstract Background Macrophages, within which Leishmania sp. replicate, generate large amounts of reactive oxygen species (ROS) and reactive nitrogen species (RNS) to kill these parasites. Methods The aim of the present study was to assess oxidative, nitrosative stresses, and some immune enzymes in blood of cutaneous leishmaniasis (CL) patients before and after treatment as well as in control individuals. Serum activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidas (GSH-Px) and the levels of reduced glutathione, malondialdehyde (MAD) and nitric oxide (NO) as well as L-arginase, myeloperoxidase (MPO), adenosine deaminase (ADA) have been studied. Results The activities of the L-arginase, MPO and ADA, the levels of MDA and NO are significantly elevated (P < 0.001), while that of SOD, CAT, and GSH-Px, and GSH level were significantly (P < 0.001) reduced in untreated patients compared with the corresponding activities of the treated and control individuals. The treatment ameliorated these agents in comparison to the untreated group but there was still variations between the values of treated and control groups. Conclusion These results suggested that oxidative and nitrusative stress may play an important role in the pathogenesis of untreated cutaneous leishmaniasis

TNF-α plays a role in sleep disturbance in patients with rotator cuff tear

2021 ◽  
Author(s):  
Chul-Hyun Cho ◽  
Du Hwan Kim ◽  
Eun Hee Baek ◽  
Du-Han Kim
Keyword(s):  
Rotator Cuff ◽  
Sleep Disturbance ◽  
Serum Levels ◽  
Control Group ◽  
Control Groups ◽  
Cuff Tear ◽  
Clinical Scores ◽  
Tnf Α ◽  
And Control ◽  
Concentration Levels

Abstract Background The purpose of this study was to determine serum levels of sleep-related cytokines in patients with rotator cuff tear (RCT) and to investigate the correlations between serum levels of sleep-related cytokines and clinical scores. Methods Peripheral blood samples were collected from 63 study participants were divided into three groups: RCT patients with sleep disturbance (sleep disturbance group; SD group) (n = 21), RCT patients without sleep disturbance (normal sleep group; NS group) (n = 21), and patients with shoulder instability (control group) (n = 21). Serum concentration levels of sleep-related cytokines including interleukin-1α (IL-1α), IL-1β, IL-2, IL-6, IL-8, IL-10, and tumor necrosis factor-α (TNF-α) were measured via enzyme-linked immunosorbent assay. The associations between serum levels of sleep-related cytokines and clinical scores including the Visual Analogue Scale (VAS) pain score, the University of California at Los Angeles (UCLA) score, and the Pittsburgh Sleep Quality Index (PSQI) were analyzed. Results Serum concentration levels of TNF-α were significantly higher in the SD group compared with those of the NS and control groups (P = < 0.001 and 0.05). Serum levels of IL-8 and IL-10 were significantly higher in the SD group compared with those of control group (P = 0.01 and = 0.05), but did not differ significantly from that of the NS group (P > 0.05). Serum level of IL-6 was significantly lower in the SD group compared with those of the NS and control groups (P = < 0.001 and 0.01). There were no associations between serum levels of sleep-related cytokines and all clinical scores including VAS pain, UCLA, and PSQI scores (all P > 0.05). Conclusion The current findings suggest that TNF-α may play a significant role in the pathophysiology of sleep disturbance in patients with RCT and be a possible therapeutic target to improve sleep disturbance in patients with RCT.

Oligoclonal bands and increased cytokine levels in idiopathic intracranial hypertension

Cephalalgia ◽  
2015 ◽  
Vol 35 (13) ◽  
pp. 1153-1161 ◽  
Author(s):  
Güneş Altıokka-Uzun ◽  
Erdem Tüzün ◽  
Esme Ekizoğlu ◽  
Canan Ulusoy ◽  
Sibel Yentür ◽  
...  
Keyword(s):  
Intracranial Hypertension ◽  
Idiopathic Intracranial Hypertension ◽  
Vision Loss ◽  
Oligoclonal Bands ◽  
Control Groups ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Immunologic Factors ◽  
Ifn Γ ◽  
And Control

Objectives The pathogenesis of idiopathic intracranial hypertension (IIH) is currently unknown and there are speculations about the contribution of some immunologic factors. The aim of this study is to investigate the presence of oligoclonal bands (OCBs) and cerebrospinal fluid (CSF) and/or serum cytokine levels in patients with IIH. Methods Patients fulfilling revised diagnostic criteria for IIH were included. Their demographic, clinical, ophthalmologic and laboratory features were examined. Serum and CSF samples were detected by isoelectric focusing and immunoblotting for OCBs. The samples of IIH patients and control groups were investigated by ELISA for cytokine levels. Results We detected OCBs in eight (30.77%) patients diagnosed with IIH. There were no other obvious clinical and laboratory differences of IIH profiles between the patients with and without OCBs, but frequency of vision loss was significantly higher in the group with OCBs in comparison to OCB negatives ( p = 0.038). Patients with IIH had highly elevated TNF-α, IFN-γ, IL-4, IL-10, IL-12, IL-17 in their sera compared to patients with multiple sclerosis (MS) and healthy controls. Furthermore, all cytokines except TNF-α in the CSF were found significantly higher in IIH patients compared to MS controls. Conclusion The presence of OCBs and elevated cytokine levels in IIH patients may support an immunologic background in the pathophysiological pathway of this disorder.

scholarly journals Mesenchymal stem cell-derived exosomes protect trabecular meshwork from oxidative stress

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ying-chao Li ◽  
Juan Zheng ◽  
Xi-zi Wang ◽  
Xin Wang ◽  
Wen-jing Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Survival Rate ◽  
Candidate Genes ◽  
Inflammatory Cytokines ◽  
Trabecular Meshwork ◽  
Control Groups ◽  
Beneficial Effects ◽  
Protein Levels ◽  
Trabecular Meshwork Cells ◽  
And Control

AbstractThis study aims to investigate the beneficial effects of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) on trabecular meshwork cells under oxidative stress and predict candidate genes associated with this process. Trabecular meshwork cells were pretreated with BMSC-derived exosomes for 24 h, and exposed to 0.1 mM H2O2 for 6 h. Survival rate of trabecular meshwork cells was measured with CCK-8 assay. Production of intracellular reactive oxygen species (iROS) was measured using a flow cytometer. RT-PCR and ELISA were used to detect mRNA and protein levels of inflammatory cytokines and matrix metalloproteinases (MMPs). Sequencing of RNA and miRNA for trabecular meshwork cells from Exo and control groups was performed on BGISEQ500 platform. Phenotypically, pretreatment of BMSC-derived exosomes improves survival rate of trabecular meshwork cells exposed to H2O2, reduces production of iROS, and inhibits expression of inflammatory cytokines, whereas increases expression of MMPs. There were 23 miRNAs, 307 lncRNAs, and 367 mRNAs differentially expressed between Exo and control groups. Exosomes derived from BMSCs may protect trabecular meshwork cells from oxidative stress. Candidate genes responsible for beneficial effects, such as DIO2 and HMOX1, were predicted.

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