scholarly journals Prospecção in silico de enzimas do complexo ligninocelulolítico em bacillus thuringiensis

2021 ◽  
Author(s):  
Dimitri Sokolowskei ◽  
Edvar Carneiro Da Silva Junior ◽  
Paulo Roberto Martins Queiroz
Keyword(s):  
Bacillus Thuringiensis ◽  
In Silico ◽  
Bacillus Spp ◽  
Clustal Omega

A busca por fontes de energia alternativa avançam a medida que a disponibilidade de recursos petróleo dependentes diminuem. As biomassas são matrizes orgânicas capazes de serem convertidas em energia. Bactérias do gênero Bacillus spp. são produtoras de enzimas do complexo ligninocelulolítico e apresentam grande potencial de uso na produção de biocombustíveis. Algumas espécies do gênero ainda carecem de maiores investigações na busca destas enzimas, como é o caso do Bacillus thuringiensis. Portanto, o objetivo do presente trabalho é identificar e descrever a presença de enzimas do complexo ligninocelulolítico em B. thuringiensis. Os proteomas das bactérias utilizadas no estudo foram coletados no banco de dados NCBI e os dados foram pré tratados utilizando linguagem de programação Python. Um script em VBA foi escrito para semi automatizar a procura das enzimas desejadas nos proteomas das bactérias via interface gráfica Excel. Por fim, foi utilizado o programa Clustal Omega para construção de árvore filogenética das espécies coletadas. Foram encontradas 4 diferentes enzimas no proteoma de B. Thuringiensis: 6phospho β glucosidase, ɑ glucosidase, ɑ amilase e laccase. Todas estas com potencial de degradação de biomassa, principalmente amido e lignina. Ao analisar outras espécies do gênero, foi indentificado um maior número e diversidade de enzimas do complexo ligninocelulolítico principalmente em B. amyloliquefaciens, B. licheniformis, B. velezensis e B. subtilis. Conclui se que apesar de B. thuringiensis apresentar um potencial na degradação de biomassa, outras espécies do gênero podem ser mais eficientes em aplicações reais. Esses achados ampliam o potencial biotecnológico de B. thuringiensis, antes restrito à produção de bioinseticidas e plantas resistentes à praga.

scholarly journals Antimicrobial Effects of Potential Probiotics of Bacillus spp. Isolated from Human Microbiota: In Vitro and In Silico Methods

2021 ◽  
Vol 9 (8) ◽  
pp. 1615
Author(s):  
Alfonso Torres-Sánchez ◽  
Jesús Pardo-Cacho ◽  
Ana López-Moreno ◽  
Ángel Ruiz-Moreno ◽  
Klara Cerk ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
In Silico ◽  
Ecological Impacts ◽  
Bacillus Species ◽  
Human Gut ◽  
Contaminant Exposure ◽  
Antimicrobial Effects ◽  
Human Gut Microbiota ◽  
Bacillus Spp ◽  
In Silico Methods

The variable taxa components of human gut microbiota seem to have an enormous biotechnological potential that is not yet well explored. To investigate the usefulness and applications of its biocompounds and/or bioactive substances would have a dual impact, allowing us to better understand the ecology of these microbiota consortia and to obtain resources for extended uses. Our research team has obtained a catalogue of isolated and typified strains from microbiota showing resistance to dietary contaminants and obesogens. Special attention was paid to cultivable Bacillus species as potential next-generation probiotics (NGP) together with their antimicrobial production and ecological impacts. The objective of the present work focused on bioinformatic genome data mining and phenotypic analyses for antimicrobial production. In silico methods were applied over the phylogenetically closest type strain genomes of the microbiota Bacillus spp. isolates and standardized antimicrobial production procedures were used. The main results showed partial and complete gene identification and presence of polyketide (PK) clusters on the whole genome sequences (WGS) analysed. Moreover, specific antimicrobial effects against B. cereus, B. circulans, Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Serratia marcescens, Klebsiella spp., Pseudomonas spp., and Salmonella spp. confirmed their capacity of antimicrobial production. In conclusion, Bacillus strains isolated from human gut microbiota and taxonomic group, resistant to Bisphenols as xenobiotics type endocrine disruptors, showed parallel PKS biosynthesis and a phenotypic antimicrobial effect. This could modulate the composition of human gut microbiota and therefore its functionalities, becoming a predominant group when high contaminant exposure conditions are present.

Potential gene exchange between Bacillus thuringiensis subsp. kurstaki and Bacillus spp. in soil in situ

2010 ◽  
Vol 42 (8) ◽  
pp. 1329-1337 ◽  
Author(s):  
Francesca Donnarumma ◽  
Donatella Paffetti ◽  
Guenther Stotzky ◽  
Raffaello Giannini ◽  
Cristina Vettori
Keyword(s):  
Bacillus Thuringiensis ◽  
Gene Exchange ◽  
Bacillus Thuringiensis Subsp ◽  
Bacillus Spp

Localization and in silico study of the vegetative insecticidal proteins Vip2S-Vip1S of Bacillus thuringiensis

2016 ◽  
Vol 91 ◽  
pp. 510-517
Author(s):  
Sameh Sellami ◽  
Sonia Jemli ◽  
Nouha Abdelmalek ◽  
Emna Dabbéche ◽  
Kaïs Jamoussi
Keyword(s):  
Bacillus Thuringiensis ◽  
In Silico ◽  
Insecticidal Proteins ◽  
In Silico Study

THE TAXONOMY OF INSECT PATHOGENS RELATED TO BACILLUS CEREUS FRANKLAND AND FRANKLAND

1958 ◽  
Vol 4 (5) ◽  
pp. 531-541 ◽  
Author(s):  
A. M. Heimpel ◽  
T. A. Angus
Keyword(s):  
New Species ◽  
Bacillus Thuringiensis ◽  
Bacillus Cereus ◽  
Type Species ◽  
Identification Key ◽  
New Variety ◽  
Bacillus Spp ◽  
Insect Pathogens

The characteristics of seven crystal-forming Bacillus spp. are compared and an identification key is presented. It is concluded that the following changes in nomenclature are desirable: Bacillus thuringiensis Berliner becomes the type species Bacillus thuringiensis var. thuringiensis n. comb.; Bacillus sotto Ishiwata becomes Bacillus thuringiensis var. sotto n. comb.; and Bacillus cereus var. alesti Toumanoff and Vago becomes Bacillus thuringiensis var. alesti.Two new species, Bacillus entomocidus n. sp. and Bacillus finitimus n. sp., and a new variety, Bacillus entomocidus var. subtoxicus n. sp., are described.

scholarly journals PROSPEK PEMANFAATAN BIOPESTISIDA BAKTERI ENTOMOPATOGENIK ISOLAT LOKAL SEBAGAI AGEN PENGENDALI HAYATI HAMA TANAMAN SAYURAN

EUGENIA ◽  
2013 ◽  
Vol 19 (1) ◽  
Author(s):  
Ch. L. Salaki ◽  
D. Tarore ◽  
G. Manengkey
Keyword(s):  
Bacillus Thuringiensis ◽  
Plutella Xylostella ◽  
Spodoptera Litura ◽  
Vegetable Crops ◽  
Entomopathogenic Bacteria ◽  
Crocidolomia Binotalis ◽  
Potential Pathogen ◽  
Bacillus Spp

ABSTRACT The utilization efforts of entomopathogenic bacteria as an insecticide is still being developed. One of the potential pathogen, which is developed as a source of insecticide is Bacillus spp. The study aims to determine the level of pathogenicity, and get the pathogenity spectrum isolates in the high virulences against the pests of vegetable crops to be used as a biopesticide candidates. Testing the power to kill larvae isolates of Bacillus thuringiensis against Crocidolomia binotalis, Plutella xylostella and Spodoptera litura was performed by the Method of Ohba and Aizawa. The results showed that, of 21 local isolates B. thuringiensis, there were 15 isolates could cause the mortality of > 50% of the larvae of C. binotalis, 20 isolates toward larvae of P. xylostella and 12 isolates toward larvae of S. litura. The isolates, which could potentially be selected based on the pathogenicity, the candidates will then be developed into a biopesticide for pests control of Crocidolomia binotalis, Plutella xylostella and Spodoptera litura on vegetable crops. Keywords: biopesticide, entomopathogenic bacteria, vegetable plants     ABSTRAK Upaya pemanfaatan bakteri entomopatogenik sebagai insektisida masih terus dikembangkan. Salah satu patogen yang berpotensi untuk dikembangkan sebagai sumber insektisida adalah bakteri Bacillus spp. Penelitian bertujuan untuk mengetahui tingkat patogenisitas, spektrum patogensisitas dan mendapatkan isolat yang memiliki virulensi yang tinggi terhadap hama tanaman sayuran untuk dijadikan sebagai kandidat biopestisida. Pengujian daya bunuh isolat Bacillus thuringiensis terhadap larva uji Crocidolomia binotalis, Plutella xylostella dan Spodoptera litura dilakukan dengan Metode Ohba dan Aizawa. Hasil penelitian menunjukkan bahwa dari 21 isolat B. thuringiensis lokal terdapat 15 isolat yang dapat menyebabkan mortalitas > 50 % terhadap larva C. binotalis, 20 isolat terhadap larva P. xylostella dan 12 isolat terhadap larva S. litura. Isolat yang berpotensi selanjutnya akan diseleksi berdasarkan patogenisitasnya kemudian akan dikembangkan menjadi kandidat biopestisida untuk mengendalikan hama Crocidolomia binotalis, Plutella xylostella dan Spodoptera litura pada tanaman sayuran. Kata kunci : biopestisida, bakteri entomopatogenik, tanaman sayuran

scholarly journals Aplicaciones de la bacteria entomopatógena Bacillus thuringiensis en el control de fitopatógenos

2011 ◽  
Vol 12 (2) ◽  
pp. 129 ◽  
Author(s):  
Bertha Carreras S.
Keyword(s):  
Bacillus Thuringiensis ◽  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Control Strategies ◽  
Wide Spectrum ◽  
Control Plant ◽  
Trichoderma Spp ◽  
Pseudomonas Spp ◽  
Biological Insecticide ◽  
Bacillus Spp

<p>El uso excesivo de plaguicidas químicos provoca resis tencia en los fitopatógenos, influencia negativa sobre el ambiente y la salud humana, por lo que se impone la implantación de estrategias de control de microorganismos benéficos, como Trichoderma spp., Bacillus spp., Pseudomonas spp. y otros agentes promisorios. Bacillus thuringiensis es el insecticida biológico más utilizado en el mundo para controlar diversos insectos y organismos plaga que afectan la agricultura, la actividad forestal y que transmiten patógenos a humanos y animales. En Cuba, los productos a base de B. thuringiensis se obtienen y se utilizan desde la década de los setenta del pasado siglo, y representan más del 40% de todos los controles biológicos. Actualmente, en el Instituto de Investigaciones de Sanidad Vegetal (INISAV) se cuenta con cepas de esta especie con efectos contra diferentes insectos y organismos plaga, lo cual permite disponer de una colección que cubre un espectro de numerosas plagas que afectan varios cultivos agrícolas; sin embargo, a pesar de la especificidad, virulencia, seguridad y potencia de estas cepas contra organismos patógenos, su potencial antifúngico es desconocido, aunque se sabe que esta bacteria produce una gran diversidad de metabolitos que resultan inhibitorios de hongos fitopatógenos. En esta revisión se citan las aplicaciones de B. thuringiensis en el control de organismos fitopatógenos y de la misma se deriva la importancia de explorar estas potencialidades en las cepas que conforman la colección de B. thuringiensis del INISAV como una alternativa más al control de fitopatógenos en Cuba.</p><p> </p><p><strong>Applications of entomopathogenic bacteria Bacillus thuringiensis to control phytopathogens.</strong></p><p>The overuse of chemical pesticides causes esistance in phytopathogens and negative influences on the environment and human health; therefore, the implementation of control strategies of beneficial microorganisms such as Trichoderma spp., Bacillus spp., Pseudomonas spp., and other promising agents is compelling. Bacillus thuringiensis is the most widely used biological insecticide in the world; it controls various insects and pests that affect agriculture and forestry and transmit pathogens to humans and animals. In Cuba, products based on B. thuringiensis were obtained and have been used since the 1970s, and they represent over 40% of all biological controls. Currently, the Plant Health Research Institute (INISAV) has various strains of this species that affect insects and pest organisms in different ways. This means they have a collection that covers a wide spectrum of the many diseases affecting various agricultural crops at their disposal. However, in spite of the recognized specificity, virulence, safety, and potency of these strains against pathogens, their antifungal potential is unknown, although it is known that this bacterium produces a variety of metabolites that inhibit fungal pathogens. In this review, we explore applications B. thuringiensis to control plant pathogenic organisms; from this, we derive the importance of exploring this potential in strains that make up the INISAV collection of B. thuringiensis as an alternative method to control plant pathogens in Cuba.</p>

Biochemical, molecular and in silico characterization of arsenate reductase from Bacillus thuringiensis KPWP1 tolerant to salt, arsenic and a wide range of pH

2021 ◽  
Vol 204 (1) ◽  
Author(s):  
Paromita Banerjee ◽  
Ananya Chatterjee ◽  
Sushmita Jha ◽  
Nirbhay K. Bhadani ◽  
Partha P. Datta ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
In Silico ◽  
Arsenate Reductase ◽  
Wide Range ◽  
In Silico Characterization
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