The value of potassium, pH and D-dimer levels in early diagnosis of acute mesenteric ischemia: an experimental study on rats

IntroductionThe aim of this randomized controlled experimental study was to evaluate the efficacy of potassium, pH and D-dimer levels in blood, as well as potassium and pH levels in peritoneal lavage fluid, in the early diagnosis of acute mesenteric ischemia.Material and methodsThis study was conducted at the Istanbul University Center of Experimental Medicine after having received approval from the Istanbul University animal testing ethics committee. Male albino Wistar rats (n = 24; 250 to 350 g) were divided into two control groups and two ischemic groups. Levels of potassium, pH, and D-dimer in blood and levels of potassium and pH in peritoneal lavage fluid were analyzed for 1 h and 2 h after the induced acute mesenteric ischemia procedure. The degree of ischemic injury was determined using the histopathological damage score in tissue samples taken from the terminal ileum.ResultsIschemic groups had statistically significant differences in potassium and pH in blood and peritoneal lavage fluid compared to non-ischemic groups (p < 0.05). There was no significant difference between control and ischemic groups in terms of D-dimer and histologic grading results after 1 h (p = 0.132, p = 0.475 respectively), while there was a significant difference between control and ischemic groups after 2 h (p < 0.05).ConclusionsThe levels of potassium, pH, and D-dimer could be useful in daily practice for the early diagnosis of acute mesenteric ischemia.

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D-dimer as a marker for early diagnosis of acute mesenteric ischemia

Thrombosis Research ◽

10.1016/j.thromres.2005.04.025 ◽

2006 ◽

Vol 117 (4) ◽

pp. 463-467 ◽

Cited By ~ 37

Author(s):

Hüseyin Altinyollar ◽

Mustafa Boyabatli ◽

Uğur Berberoğlu

Keyword(s):

Early Diagnosis ◽

Mesenteric Ischemia ◽

Acute Mesenteric Ischemia ◽

D Dimer

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D-Dimer in the Early Diagnosis of Acute Mesenteric Ischemia Secondary to Arterial Occlusion in Rats

European Surgical Research ◽

10.1159/000087866 ◽

2005 ◽

Vol 37 (4) ◽

pp. 216-219 ◽

Cited By ~ 21

Author(s):

Y. Kurt ◽

M.L. Akin ◽

S. Demirbas ◽

A.H. Uluutku ◽

M. Gulderen ◽

...

Keyword(s):

Early Diagnosis ◽

Mesenteric Ischemia ◽

Arterial Occlusion ◽

Acute Mesenteric Ischemia ◽

D Dimer

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The value of combined elevation of D-dimer and neopterin as a predictive parameter for early stage acute mesenteric ischemia: An experimental study

Vascular ◽

10.1177/1708538116652267 ◽

2016 ◽

Vol 25 (2) ◽

pp. 163-169 ◽

Cited By ~ 3

Author(s):

Ali Kagan Coskun ◽

Zekai Halici ◽

Akgun Oral ◽

Yasin Bayir ◽

Ferhat Deniz ◽

...

Keyword(s):

Experimental Study ◽

Superior Mesenteric Artery ◽

Mesenteric Artery ◽

Mesenteric Ischemia ◽

Early Stage ◽

Acute Mesenteric Ischemia ◽

Artery Occlusion ◽

Control Group ◽

Blood Samples ◽

D Dimer

Background The diagnosis of acute mesenteric ischemia is variable. Early diagnosis is important for reducing the mortality and morbidity rates. Aim This experimental study aims to investigate the diagnostic utility of D-dimer and neopterin as a marker for the early stage of acute mesenteric ischemia caused by occlusion of superior mesenteric artery. Methods The levels of D-dimer and neopterin were measured using an animal acute mesenteric ischemia model in 21 male rabbits. Superior mesenteric artery occlusion (Group 1, n = 14) and control (Group 2, n = 7) groups were identified. Blood samples at different times are collected from each rabbits. Blood samples from superior mesenteric artery occlusion group were taken 30 min after anesthesia but before laparotomy, 1, 2, and 3 h after superior mesenteric artery ligation. Blood samples from control group were taken 1 h before, 1 and 3 h after anesthesia and laparotomy. The D-dimer and neopterin levels of each blood sample were measured. Results The probability of acute mesenteric ischemia was found to be 36 times higher when the D-dimer level was over 0.125 ng/L, whereas the probability was 19.2 times higher when the neopterin level was over 1.25 nmol/L. Conclusions In this experimental study, the combined elevation of two significant markers, D-dimer and neopterin, may be helpful for the early diagnosis of acute mesenteric ischemia.

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The place of D-dimer and L-lactate levels in the early diagnosis of acute mesenteric ischemia

Bratislava Medical Journal ◽

10.4149/bll_2015_094 ◽

2015 ◽

Vol 116 (05) ◽

pp. 343-350

Author(s):

B. Aydin ◽

M. Ozban ◽

M. Serinken ◽

B. Kaptanoglu ◽

N. C. Demirkan ◽

...

Keyword(s):

Early Diagnosis ◽

Mesenteric Ischemia ◽

Acute Mesenteric Ischemia ◽

Lactate Levels ◽

D Dimer

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Fibrinopeptide-A and fibrinopeptide-B may help to D-dimer as early diagnosis markers for acute mesenteric ischemia

Turkish Journal of Biochemistry ◽

10.1515/tjb-2018-0301 ◽

2019 ◽

Vol 44 (5) ◽

pp. 654-660

Author(s):

Murat Cikot ◽

Eyup Gemici ◽

Nilgun Isiksacan ◽

Osman Kones ◽

Pinar Kasapoglu ◽

...

Keyword(s):

Early Diagnosis ◽

Mesenteric Ischemia ◽

Acute Mesenteric Ischemia ◽

Control Group ◽

Albino Rats ◽

Fibrinopeptide A ◽

Mortality And Morbidity ◽

Ischemia Group ◽

Wistar Albino Rats ◽

D Dimer

Abstract Background The aim of this study was to investigate the importance of fibrinopeptide-A and fibrinopeptide-B, which occur during the formation of D-dimer, the most commonly used laboratory parameter, in the early diagnosis of acute mesenteric ischemia (AMI). Materials and methods This experimental study was performed in 30 male pathogen-free Wistar albino rats. The experimental animals were divided into 3 equal groups: Control group (n = 10), Sham group (n = 10) and Ischemia group (n = 10). Blood samples were taken 0, 1, 3, and 6 h after the simulation of mesenteric ischemia. Results Fibrinopeptide-A and fibrinopeptide-B levels increased significantly in the first 6 h in the ischemic group, similar to the increase in D-dimer levels. The statistical change between 0, 1, 3 and 6 h was more significant for fibrinopeptide-A and fibrinopeptide-B. Conclusion Fibrinopeptide-A and fibrinopeptide-B may be markers that can be used for early diagnosis of mesenteric ischemia, early diagnosis is highly important for decreasing mortality and morbidity.

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Role and Regulation of Adipokines during Zymosan-Induced Peritoneal Inflammation in Mice

Endocrinology ◽

10.1210/en.2008-0327 ◽

2008 ◽

Vol 149 (8) ◽

pp. 4080-4085 ◽

Cited By ~ 20

Author(s):

Maria Pini ◽

Melissa E. Gove ◽

Joseph A. Sennello ◽

Jantine W. P. M. van Baal ◽

Lawrence Chan ◽

...

Keyword(s):

Inflammatory Infiltrate ◽

Peritoneal Lavage ◽

Lavage Fluid ◽

Wild Type ◽

Cellular Infiltrate ◽

Peritoneal Lavage Fluid ◽

Peritoneal Inflammation ◽

Leptin Deficiency ◽

Cytokine Levels

Adipokines, cytokines mainly produced by adipocytes, are active participants in the regulation of inflammation. Administration of zymosan (ZY) was used to investigate the regulation and role of adipokines during peritonitis in mice. Injection of ZY led to a significant increase in leptin levels in both serum and peritoneal lavage fluid, whereas a differential trend in local vs. systemic levels was observed for both resistin and adiponectin. The role of leptin in ZY-induced peritonitis was investigated using leptin-deficient ob/ob mice, with and without reconstitution with exogenous leptin. Leptin deficiency was associated with delayed resolution of peritoneal inflammation induced by ZY, because ob/ob mice had a more pronounced cellular infiltrate in the peritoneum as well as higher and prolonged local and systemic levels of IL-6, TNFα, IL-10, and chemokine (C-X-C motif) ligand 2 compared with wild-type mice. Reconstitution with exogenous leptin exacerbated the inflammatory infiltrate and systemic IL-6 levels in ob/ob mice while inhibiting production of TNFα, IL-10, and chemokine (C-X-C motif) ligand 2. In contrast with the important role of leptin in regulating each aspect of ZY-induced peritonitis, adiponectin deficiency was associated only with a decreased inflammatory infiltrate, without affecting cytokine levels. These findings point to a complex role for adipokines in ZY-induced peritonitis and further emphasize the interplay between obesity and inflammation.

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Mean Platelet Volume: Is It a Predictive Parameter in Diagnosis of Acute Mesenteric Ischemia?

International Surgery ◽

10.9738/intsurg-d-14-00268.1 ◽

2015 ◽

Vol 100 (5) ◽

pp. 962-965 ◽

Cited By ~ 3

Author(s):

Ahmet Türkoğlu ◽

Mesut Gül ◽

Abdullah Oğuz ◽

Zübeyir Bozdağ ◽

Burak Veli Ülger ◽

...

Keyword(s):

Early Diagnosis ◽

Mean Platelet Volume ◽

Mesenteric Ischemia ◽

Area Under The Curve ◽

Acute Mesenteric Ischemia ◽

The Elderly ◽

Control Group ◽

Characteristic Analysis ◽

Platelet Volume ◽

Cell Counts

Our objective for this study was to discuss the usability of mean platelet volume, which is associated with numerous vascular pathologies, in the early diagnosis of acute mesenteric ischemia. Acute mesenteric ischemia is an uncommon, life-threatening clinical condition mostly seen in the elderly. Early diagnosis of acute mesenteric ischemia and correction of blood circulation before necrosis occurs are important factors affecting prognosis. A total of 95 patients who underwent emergency surgery for acute mesenteric ischemia and 90 healthy volunteers as control group were included in this study. Age, gender, hemoglobin values, white blood cell counts, mean platelet volume, and platelet counts are recorded for evaluation. The mean platelet volume values were significantly higher in patients with acute mesenteric ischemia than in the controls (9.4 ± 1.1 fL and 7.4 ± 1.4 fL, respectively; P < 0.001). Receiver-operating characteristic analysis demonstrated a cutoff value of mean platelet volume as 8.1 fL (area under the curve, 0.862), a sensitivity of 83.2%, and a specificity of 80%. As a result, in the patients who are admitted to the hospital with acute nonspecific abdominal pain and suspected of having acute mesenteric ischemia, high mean platelet volume values in routine hemograms support the diagnosis of acute mesenteric ischemia.

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