scholarly journals Gold nanoparticles formulated with Nigella aqueous extract having potent antioxidant ‎and ‎anti-human ovarian cancer activities in vitro condition

2021 ◽  
Author(s):  
Ju Li ◽  
Khodabakhsh Rashidi ◽  
Behnam Mahdavi ◽  
Samaneh Goorani ◽  
Mohammad Karimian ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gold Nanoparticles ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Treatment Strategies ◽  
Human Ovarian Cancer ◽  
Dna And Rna ◽  
Ovarian Cancer Cell Lines ◽  
Nigella Damascena

IntroductionRecently, various nanoparticles containing medicinal plants have been specifically designed to deliver anticancer drugs and nucleic acids such as DNA and RNA to cancer cells and as a result, they open up new avenues in cancer treatment strategies. In this study, gold nanoparticles were synthesized in aqueous medium using Nigella damascena extract as ‎stabilizing and reducing agents. ‎Material and methodsThe synthesized nanoparticles (AuNPs) were characterized using different techniques including UV-Vis. and ‎FT-IR spectroscopy, and Transmission electron microscopy (TEM). TEM images exhibited a uniform spherical ‎morphology in size of 21.64 nm for the biosynthesized nanoparticles. In the cellular and molecular part of the ‎recent study, the treated cells with AuNPs were assessed by MTT assay for 48h about the cytotoxicity and anti-‎human ovarian cancer ‎ properties on normal (HUVEC) and ovarian cancer cell lines i.e. PA-1, Caov-3, SW 626, ‎and SK-OV-3. ‎ResultsThe viability of malignant ovarian‎ cell line reduced dose-dependently in the presence of AuNPs. The IC50 of ‎AuNPs were 232‎, ‎204‎, ‎193‎, and ‎288 µg/mL against PA-1, Caov-3, SW 626, and SK-OV-3 cell lines, ‎respectively. In the antioxidant test, the IC50 of AuNPs and BHT against DPPH free radicals were 151 and 142 ‎‎µg/mL, respectively. ‎ConclusionsAfter clinical study, gold nanoparticles containing Nigella damascena leaf aqueous extract may be used to ‎formulate a new chemotherapeutic drug or supplement to treat the several types of human ovarian cancer.‎

scholarly journals Anti-human ovarian cancer, cytotoxicity, and antioxidant effects of Nigella Sativa green-formulated Au nanoparticles: Describing a new chemotherapeutic supplement

2021 ◽  
Author(s):  
Haibo Ruan ◽  
Li Wang ◽  
Minyuan Wang ◽  
Weiwei Mo ◽  
Jichao Jin ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Ovarian Cancer ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Nigella Sativa ◽  
Antioxidant Properties ◽  
Butylated Hydroxytoluene ◽  
Human Ovarian Cancer ◽  
Chemotherapy Drugs

IntroductionThe recent research showed that the Gold nanoparticles (GNPs) formulated with Nigella Sativa aqueous extract having potent antioxidant and anti-human ovarian cancer activities in vitro condition.Material and methodsFor determinate the properties of the GNPs that were produced from the reaction between gold chloride solution with aqueous Nigella Sativa extract, we used UV–Visible Spectroscopy (UV-Vis), Field Emission Scanning Electron Microscopy (FE‐SEM), Fourier Transformed Infrared Spectroscopy (FT‐IR), and Transmission Electron Microscopy (TEM). For evaluating anti-ovarian cancer and cytotoxicity effects of GNPs, Au chloride, and Nigella Sativa aqueous extract, we used MTT assay.ResultsThe result of this test showed that GNPs have no cytotoxicity on normal cell line (HUVEC) and have potent anti-ovarian cancer features dose-dependently against PA-1, SK-OV-3, and SW-626 cell lines. The IC50 of GNPs were 249, 361, and 433 µg/mL against PA-1, SW-626, and SK-OV-3 cell lines, respectively. For evaluating the antioxidant features of GNPs, Au chloride, and Nigella Sativa aqueous extract, we used the DPPH test, in this test butylated hydroxytoluene was a positive control, the results of this test showed that the GNPs have an effective antioxidant feature. In the antioxidant test, the IC50 of GNPs and BHT were 144 and 201 µg/mL, respectively.ConclusionsProbably, potent anti-human ovarian cancer activities of GNPs formulated with Nigella Sativa aqueous seed extract because of antioxidant properties. After evaluating the effectiveness of this formulation in clinical trial researches, it can be a good alternative to chemotherapy drugs.

scholarly journals Difference between genomic actions of estrogen versus raloxifene in human ovarian cancer cell lines

Oncogene ◽  
2008 ◽  
Vol 27 (19) ◽  
pp. 2737-2745 ◽  
Author(s):  
H Sasaki ◽  
J Hayakawa ◽  
Y Terai ◽  
M Kanemura ◽  
A Tanabe-Kimura ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Cancer Cell Lines ◽  
Human Ovarian Cancer ◽  
Human Ovarian Cancer Cell ◽  
Ovarian Cancer Cell Lines

scholarly journals miR-214 promotes radioresistance in human ovarian cancer cells by targeting PETN

2017 ◽  
Vol 37 (4) ◽  
Author(s):  
Qin Zhang ◽  
Shuxiang Zhang
Keyword(s):  
Ovarian Cancer ◽  
Ionizing Radiation ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Cancer Cell Lines ◽  
Ovarian Cancer Cells ◽  
Human Ovarian Cancer ◽  
Ovarian Cancer Cell Lines ◽  
Cancer Tissues

Ovarian cancer is one of the leading causes of death among gynecological malignancies. Increasing evidence indicate that dysregulation of microRNAs (miRNAs) plays an important role in tumor radioresistance. The aim of the present study is to investigate whether microRNA-214 (miR-214) was involved in radioresistance of human ovarian cancer. Here, we showed that miR-214 was significantly up-regulated in ovarian cancer tissues and radioresistance ovarian cancer cell lines. Transfection of miR-214 agomir in radiosensitive ovarian cancer cell lines promoted them for resistance to ionizing radiation, whereas transfection of miR-214 antagomir in radioresistance ovarian cancer cell lines sensitized them to ionizing radiation again. Furthermore, we found miR-214 effectively promoted tumor radioresistance in xenograft animal experiment. Western blotting and quantitative real-time PCR demonstrated that miR-214 negatively regulated PTEN in radioresistance ovarian cancer cell lines and ovarian cancer tissues. Taken together, our data conclude that miR-214 contributes to radioresistance of ovarian cancer by directly targeting PTEN.

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