Staining Cells with Extracts Prepared from Flowers of Bougainvillea X Buttiana.

2020 ◽  
Author(s):  
◽  
Paul Towet
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Red Blood Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
Human Red Blood Cells ◽  
Human Blood Cells ◽  
Background Staining ◽  
Gram Negative Organisms ◽  
The University

Background: Staining is the application of dyes to specimens to impart colour to cells through a chemical reaction. The study aimed at finding plant extracts to stain human blood cells, stem sections of Amaranthus species, Gram-negative organisms such as Escherichia coli, and Gram-positive organisms such as Staphylococcus aureus. Methodology: Healthy mature flowers of Bougainvillea X buttiana and Amaranthus species plants were picked from gardens around the University of Kisubi. Bracts of Bougainvillea X buttiana were separated from other flower parts and air-dried. Both negative and positive controls for cells were prepared. Results: White blood cells, platelets, and cells of Escherichia Coli and Staphylococcus aureus did not stain under all treatments with the extracts while human red blood cells and stem sections of Amaranthus species stained under certain treatments with the extracts. The extracts were more successful in staining stem sections of Amaranthus species as compared to human red blood cells where staining occurred in very few circumstances. Stem sections of Amaranthus species required shorter to stain effectively while human red blood cells required longer to stain effectively. Conclusion: Extracts of the bracts of Bougainvillea X  can be experimented with various cells when their pH is neutral and alkaline.

scholarly journals Staining Cells with Extracts Prepared from Flowers of Bougainvillea X Buttiana.

2020 ◽  
Author(s):  
◽  
Paul Towet
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Red Blood Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
Human Red Blood Cells ◽  
Human Blood Cells ◽  
Background Staining ◽  
Gram Negative Organisms ◽  
The University

Abstract Background: Staining is the application of dyes to specimens to impart colour to cells through a chemical reaction. The study aimed at finding plant extracts to stain human blood cells, stem sections of Amaranthus species, Gram-negative organisms such as Escherichia coli, and Gram-positive organisms such as Staphylococcus aureus. Methodology: Healthy mature flowers of Bougainvillea X buttiana and Amaranthus species plants were picked from gardens around the University of Kisubi. Bracts of Bougainvillea X buttiana were separated from other flower parts and air-dried. Both negative and positive controls for cells were prepared. Results: White blood cells, platelets, and cells of Escherichia Coli and Staphylococcus aureus did not stain under all treatments with the extracts while human red blood cells and stem sections of Amaranthus species stained under certain treatments with the extracts. The extracts were more successful in staining stem sections of Amaranthus species as compared to human red blood cells where staining occurred in very few circumstances. Stem sections of Amaranthus species required shorter to stain effectively while human red blood cells required longer to stain effectively. Conclusion: Extracts of the bracts of Bougainvillea X can be experimented with various cells when their pH is neutral and alkaline.

scholarly journals STUDIES ON HEMAGGLUTINATION AND HEMOLYSIS BY ESCHERICHIA COLI ANTISERA

1952 ◽  
Vol 96 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Erwin Neter ◽  
Lee F. Bertram ◽  
Dorothy A. Zak ◽  
Miriam R. Murdock ◽  
Carl E. Arbesman
Keyword(s):  
Escherichia Coli ◽  
Guinea Pig ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Homologous Blood ◽  
E Coli ◽  
Blood Group A ◽  
Human Red Blood Cells ◽  
Order Of Magnitude ◽  
Group A

A study on hemagglutination and hemolysis by Escherichia coli O111 and O55 (rabbit) antisera and on hemagglutination and hemolysis inhibition by E. coli O111 and O55 antigens revealed the following facts. 1. Red blood cells of man, dog, rabbit, guinea pig, sheep, rat, and chicken adsorb E. coli O111 and O55 antigens and thus become specifically agglutinable by the homologous E. coli antisera. 2. The adsorption of these E. coli antigens is a function of the concentration of the antigen, the time (from 5 minutes to 2 hours) of treatment of the red blood cells with the antigen, and the concentration of the red blood cells used. 3. Red blood cells of man and sheep adsorb simultaneously both antigens, as indicated by the fact that both antisera give agglutination of all red blood cells. Complete agglutination does not occur when a mixture of red blood cells treated separately with the two antigens is added to one or the other of the two antisera. 4. Treatment of red blood cells of man with one of the antigens does not block the adsorption of the second antigen. Human cells treated with either or both antigens are still agglutinated by the homologous blood group (A, B, and Rh)-specific antibodies. 5. In the presence of guinea pig complement, E. coli O111 and O55 antisera produce hemolysis of modified human red blood cells in titers of the same order of magnitude as those giving hemagglutination and bacterial agglutination. The same antisera produce hemolysis of sheep cells treated with the identical antigens in titers exceeding by far those giving agglutination of modified human or sheep red blood cells. 6. Both sediment and supernate of a boiled E. coli suspension are capable of modifying red blood cells for E. coli hemagglutination; in contrast, the supernate obtained from an unboiled suspension and then heated does not modify red blood cells for hemagglutination, although it contains the antigen which can specifically adsorb E. coli antibodies, as shown by means of the hemagglutination and hemolysis inhibition tests. 7. Both the unheated and the boiled suspensions of E. coli O111 and O55 inhibit hemagglutination and hemolysis specifically. 8. Rabbit red blood cells modified by either E. coli O111 or 055 antigens, upon intravenous injection into rabbits, engender specific E. coli antibodies. The significance of the results is discussed.

Stabilization of Human Erythrocytes for Lyophilization: Recovery of Biochemical Activities and Membrane Filterability.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3670-3670
Author(s):  
Shang-Zhi Xu ◽  
Arthur P. Bode
Keyword(s):  
Red Blood Cells ◽  
Pore Size ◽  
Blood Cells ◽  
White Blood Cells ◽  
Osmotic Fragility ◽  
Homocysteic Acid ◽  
Human Red Blood Cells ◽  
Before And After ◽  
Pass Through ◽  
2,3 Dpg

Abstract It has much more advantages to preserve human red blood cells (RBC) in freezing and drying form than conventional blood storage, even though it is still under developing. In the present study, we successfully used 0.045% glutaraldehyde(GA) to fix RBCs under appropriate conditions prior to lyophilization and regained the bulk of RBCs membrane filterability. The phlebotomized whole blood was anti-coagulated with CPDA-1 and the plasma was removed by centrifugation. White blood cells were removed using Leucocytes filter (Pall Corporation). 1 x 10 9 cells/ml were separately incubated with 0.03%, 0.045% and 0.05% GA at 37 °C for 10 min; RBCs at concentrations 0.5 x 10 9, 1 x 10 9 and 2 x 10 9 cell/ml were incubated with 0.045% GA at 37 °C for 10 min; Bovine serum albumin (BSA), trehalose, hydroxyethyl starch and dextran were used as protective additive in freezing and drying RBCs, respectively; ethanolamine, glycine, lysine, BSA, glutamic acid and homocysteic acid were used to recover membrane deformability due to GA fixation. Recovery RBCs yield after reconstituion was determined by a cell counter. Fee hemoglobin , glutathione (GSH), 2,3-diphosphoglycerate (2,3-DPG), ATP, and Glucose-6-phosphate dehydrogenase (G-6-PDH) in RBCs were determined before and after lyophilization. Membrane flexibility was assayed by osmotic fragility test. RBCs filterability was determined using positive filter apparatus through 5 micrometer pore size nitrocellulose membrane. Our results suggested that RBCs at 1 x 10 9 cells/ml fixed by 0.045% GA for10 min at pH 7.8 were enough to resist freezing and drying damages and the bulk of RBCs’s membrane filterability were remained. The effective fixation of GA is dependent on GA’s concentration, RBCs concentration, incubation period and pH etc. BSA is the most potential additive in preserving RBCs. More interestingly, the following reagents can recover the reduced RBCs membrane filterability by GA fixation. The potency of recovery is in the order of : 10 mM ethanolamine > 5 mM lysine > 0.5%BSA > 10 mM glutamatic acid > 5 mM homcysteic acid > 2% glycine. After reconstitution, 85 ± 2.3% RBCs yield is achieved, 71 ± 4.1% of these RBCs can freely pass through 5 micrometer pore size filter membrane. Biochemical function indexes as GSH, 2,3-DPG, ATP, and G-6-PDH are saved by 20 ± 1.2%. Lyophilized human red blood cells yields from reconstitution by different treatment. 1x 109 cells/ml fixed at 0.03% glutaraldehyde 44.7 ± 4.1 0.045%glutaraldehyde 84.7 ± 5.8 0.05%glutaraldehyde 88.7 ± 7.6 0.045% glutaraldehyde fix: 0.5x 109 cells/ml 91 ± 4.3 1x 109 cells/ml 84.7 ± 5.8 2 x 109 cells/ml 35.2 ± 2.3 Effects of various treatment on lyophilized RBCs after reconstitution. Group Filterability(%) 1x9 cells/ml fixed by: N = 5. 0.03%glutaraldehyde 88.3 ± 1.2 0.045%glutaraldehyde 73.3 ± 1.8 0.05%glutaraldehyde 44.7 ± 2.8 0.045%glutaraldehyde fixation at: 0.5x109 cells/ml 0 1x109 cells/ml 73.3 ± 1.8 2x109 cells/ml 83.9 ± 4.6

THE ENTEROBACTERIAL HEMAGGLUTINATION TEST AND ITS DIAGNOSTIC POTENTIALITIES

1956 ◽  
Vol 2 (3) ◽  
pp. 232-244 ◽  
Author(s):  
E. Neter ◽  
E. A. Gorzynski ◽  
R. M. Gino ◽  
O. Westphal ◽  
O. Lüderitz
Keyword(s):  
Escherichia Coli ◽  
Red Blood Cells ◽  
Blood Cells ◽  
High Titer ◽  
Agglutination Test ◽  
Salmonella Infection ◽  
Hemagglutination Test ◽  
Human Red Blood Cells ◽  
Sensitive Tool ◽  
Bacterial Agglutination

Crude Salmonella antigens obtained from heated cultures are readily adsorbed on human red blood cells; the latter are specifically agglutinated by homologous bacterial antibodies, This Salmonella hemagglutination test is shown to be a sensitive tool for the detection of antibodies developed during salmonellosis and is distinctly superior to the conventional bacterial agglutination test. Patients with Salmonella infection may develop homologous hemagglutinins in high titer and a variety of heterologous enteric antibodies in low titer. These antibodies can be differentiated by absorption with modified red blood cells. Three different purified Escherichia coli lipopolysaccharides as well as the corresponding crude antigens can be simultaneously adsorbed on red blood cells. A suspension of red blood cells modified with several Salmonella and Shigella antigens is shown to be suitable as a polyvalent antigen for the detection of enterobacterial antibodies. The possible clinical usefulness of the monovalent and polyvalent enterobacterial hemagglutination tests is discussed.

scholarly journals Preparation and Evaluation of a 7-Parameter Intralaboratory Control Blood of 4-Month Stability

1983 ◽  
Vol 20 (5) ◽  
pp. 302-307 ◽  
Author(s):  
A J P F Lombarts ◽  
B Leijnse
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
Established Method ◽  
Cell Parameters ◽  
Long Term Stability ◽  
Human Red Blood Cells ◽  
Preparation And Evaluation

Sterile, fresh concentrates of human red blood cells suspended in a specially designed sterile preservation medium have a stability of all six red blood cell parameters of over four months as measured in the Coulter Model S-Plus II. Substitution under sterile conditions of the unstable white blood cells for (commercially available) fixed (human) red blood cells is a well-established method to obtain simulated white blood cell suspensions of long-term stability.

The Effect of Red Blood Cells on the ADP-induced Aggregation of Human Platelets in Flow Through Tubes

1990 ◽  
Vol 63 (01) ◽  
pp. 112-121 ◽  
Author(s):  
David N Bell ◽  
Samira Spain ◽  
Harry L Goldsmith
Keyword(s):  
Platelet Aggregation ◽  
Shear Rate ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Platelet Rich Plasma ◽  
Mean Transit Time ◽  
White Blood Cells ◽  
Aggregate Size ◽  
Human Platelets ◽  
Induced Aggregation

SummaryThe effect of red blood cells, rbc, and shear rate on the ADPinduced aggregation of platelets in whole blood, WB, flowing through polyethylene tubing was studied using a previously described technique (1). Effluent WB was collected into 0.5% glutaraldehyde and the red blood cells removed by centrifugation through Percoll. At 23°C the rate of single platelet aggregtion was upt to 9× greater in WB than previously found in platelet-rich plasma (2) at mean tube shear rates Ḡ = 41.9,335, and 1,920 s−1, and at both 0.2 and 1.0 µM ADP. At 0.2 pM ADP, the rate of aggregation was greatest at Ḡ = 41.9 s−1 over the first 1.7 s mean transit time through the flow tube, t, but decreased steadily with time. At Ḡ ≥335 s−1 the rate of aggregation increased between t = 1.7 and 8.6 s; however, aggregate size decreased with increasing shear rate. At 1.0 µM ADP, the initial rate of single platelet aggregation was still highest at Ḡ = 41.9 s1 where large aggregates up to several millimeters in diameter containing rbc formed by t = 43 s. At this ADP concentration, aggregate size was still limited at Ḡ ≥335 s−1 but the rate of single platelet aggregation was markedly greater than at 0.2 pM ADP. By t = 43 s, no single platelets remained and rbc were not incorporated into aggregates. Although aggregate size increased slowly, large aggregates eventually formed. White blood cells were not significantly incorporated into aggregates at any shear rate or ADP concentration. Since the present technique did not induce platelet thromboxane A2 formation or cause cell lysis, these experiments provide evidence for a purely mechanical effect of rbc in augmenting platelet aggregation in WB.

Gene Expression Differences in White Blood Cells after Escherichia coli Infection in Chickens

2012 ◽  
Author(s):  
Erin Sandford ◽  
Megan Orr ◽  
Xianyao Li ◽  
Huaijun Zhou ◽  
timothy J. Johnson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Blood Cells ◽  
White Blood Cells ◽  
Escherichia Coli Infection
Sign in / Sign up

Export Citation Format

Share Document