Crafting the extraordinary: site-specificity and liveness

2020 ◽  
Author(s):  
María A. Vélez-Serna
Keyword(s):  
Site Specificity ◽  
Power Struggle ◽  
Site Specific ◽  
Film Projection

‘Experiential’ forms of exhibition use liveness and site-specificity as strategies to valorize the eventfulness of an engagement with film. This chapter explores different practices and intentions of eventful cinema. It first examines liveness as a power struggle between exhibitor and text, which needs to be understood in relation to the showmanship tradition of early and classical eras. It then discusses site-specific screenings, considering different ways to modulate the encounter between environment and film projection, from the diegetically immersive to the distracted and relaxed. Finally, it returns to intermediality as a creative opportunity generated by non-theatrical exhibition.

scholarly journals Site-Specific and Trigger-Activated Modification of Proteins by Means of Catalytic Hemin/G-quadruplex (hGQ) DNAzyme Nanostructures

2020 ◽  
Author(s):  
Jordi Keijzer ◽  
Bauke Albada
Keyword(s):  
Heavy Chain ◽  
Protein Modification ◽  
Site Specificity ◽  
Site Specific ◽  
Antibody Drug Conjugates ◽  
Synthetic Dna ◽  
Drug Conjugates ◽  
Sds Page ◽  
G Quadruplex ◽  
Antibody Drug

<div>Synthetic DNA that forms various G-quadruplex nanostructures, in combination with hemin, <i>N</i>-methyl luminol derivatives, and H2O2 can site-specifically modify proteins (i.e. evidence is provided for lysozyme and human alpha-thrombin). The catalytic modification is completed in 15-30 mins, and the site-specificity is influenced by the G-quadruplex topology (a total of 22 G-quadruplex forming sequences was tested). We also show that the heavy chain of the therapeutic antibody trastuzumab is modified, which facilitates the preparation of antibody-drug conjugates. Furthermore, a trigger can be programmed into this synthetic DNA so that the protein modification chemistry is made dependent on an external trigger.</div><div><br></div>Techniques used: HPLC, SDS-PAGE, LC-MS/MS, NMR.

scholarly journals When Public Art Goes Bad: Two Competing Features of Public Art

2019 ◽  
Vol 2 (1) ◽  
pp. 1-9
Author(s):  
Mary Beth Willard
Keyword(s):  
Public Art ◽  
The Other ◽  
Site Specificity ◽  
Specific Work ◽  
Site Specific ◽  
The Public ◽  
Public Work ◽  
A Site ◽  
Compare And Contrast

AbstractNot all public art is bad art, but when public art is bad, it tends to be bad in an identifiable way. In this paper, I develop a Waltonian theory of the category of public art, according to which public art standardly is both accessible to the public and minimally site-specific. When a work lacks the standard features of the category to which it belongs, appreciators tend to perceive the work as aesthetically flawed. I then compare and contrast cases of successful and unsuccessful public art to show that accessibility and site-specificity are features which tend to preclude the other. It is difficult, although hardly impossible, for a site-specific work to remain accessible, and difficult for an accessible work to engage adequately with the site on which it is situated. As a result, while not all public art is bad, the features peculiar to public work encourage a latent tendency toward badness.

scholarly journals The post-translational modification landscape of commercial beers

2021 ◽  
Author(s):  
Edward D. Kerr ◽  
Christopher H. Caboche ◽  
Cassandra L. Pegg ◽  
Toan K. Phung ◽  
Claudia Gonzalez Viejo ◽  
...  
Keyword(s):  
Site Specificity ◽  
Foam Formation ◽  
Post Translational Modification ◽  
Post Translational Modifications ◽  
Site Specific ◽  
Quality Properties ◽  
Surface Active ◽  
Molecular Complexity ◽  
Very High ◽  
High Diversity

AbstractBeer is one of the most popular beverages worldwide. As a product of variable agricultural ingredients and processes, beer has high molecular complexity. We used DIA/SWATH-MS to investigate the proteomic complexity and diversity of 24 commercial Australian beers. While the overall complexity of the beer proteome was modest, with contributions from barley and yeast proteins, we uncovered a very high diversity of post-translational modifications (PTMs), especially proteolysis, glycation, and glycosylation. Proteolysis was widespread throughout barley proteins, but showed clear site-specificity. Oligohexose modifications were common on lysines in barley proteins, consistent with glycation by maltooligosaccharides released from starch during malting or mashing. O-glycosylation consistent with oligomannose was abundant on secreted yeast glycoproteins. We developed and used data analysis pipelines to efficiently extract and quantify site-specific PTMs from SWATH-MS data, and showed incorporating these features into proteomic analyses extended analytical precision. We found that the key differentiator of the beer glyco/proteome was the brewery, followed by the beer style. Targeting our analyses to beers from a single brewery, Newstead Brewing Co., allowed us to identify beer style-specific features of the glyco/proteome. Specifically, we found that proteins in darker beers tended to have low glycation and high proteolysis. Finally, we objectively quantified features of foam formation and stability, and showed that these quality properties correlated with the concentration of abundant surface-active proteins from barley and yeast.

scholarly journals Site-Specific and Trigger-Activated Modification of Proteins by Means of Catalytic Hemin/G-quadruplex (hGQ) DNAzyme Nanostructures

2020 ◽  
Author(s):  
Jordi Keijzer ◽  
Bauke Albada
Keyword(s):  
Heavy Chain ◽  
Protein Modification ◽  
Site Specificity ◽  
Site Specific ◽  
Antibody Drug Conjugates ◽  
Synthetic Dna ◽  
Drug Conjugates ◽  
Sds Page ◽  
G Quadruplex ◽  
Antibody Drug

<div>Synthetic DNA that forms various G-quadruplex nanostructures, in combination with hemin, <i>N</i>-methyl luminol derivatives, and H2O2 can site-specifically modify proteins (i.e. evidence is provided for lysozyme and human alpha-thrombin). The catalytic modification is completed in 15-30 mins, and the site-specificity is influenced by the G-quadruplex topology (a total of 22 G-quadruplex forming sequences was tested). We also show that the heavy chain of the therapeutic antibody trastuzumab is modified, which facilitates the preparation of antibody-drug conjugates. Furthermore, a trigger can be programmed into this synthetic DNA so that the protein modification chemistry is made dependent on an external trigger.</div><div><br></div>Techniques used: HPLC, SDS-PAGE, LC-MS/MS, NMR.

scholarly journals Stem-loop structure preference for site-specific RNA editing by APOBEC3A and APOBEC3G

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e4136 ◽  
Author(s):  
Shraddha Sharma ◽  
Bora E. Baysal
Keyword(s):  
Nucleotide Sequence ◽  
Rna Editing ◽  
Site Preference ◽  
Site Specificity ◽  
Loop Structure ◽  
The Novel ◽  
Stem Loop ◽  
Site Specific ◽  
Cytidine Deaminases ◽  
Stem Loop Structure

APOBEC3A and APOBEC3G cytidine deaminases inhibit viruses and endogenous retrotransposons. We recently demonstrated the novel cellular C-to-U RNA editing function of APOBEC3A and APOBEC3G. Both enzymes deaminate single-stranded DNAs at multiple TC or CC nucleotide sequences, but edit only a select set of RNAs, often at a single TC or CC nucleotide sequence. To examine the specific site preference for APOBEC3A and -3G-mediated RNA editing, we performed mutagenesis studies of the endogenous cellular RNA substrates of both proteins. We demonstrate that both enzymes prefer RNA substrates that have a predicted stem-loop with the reactive C at the 3′-end of the loop. The size of the loop, the nucleotides immediately 5′ to the target cytosine and stability of the stem have a major impact on the level of RNA editing. Our findings show that both sequence and secondary structure are preferred for RNA editing by APOBEC3A and -3G, and suggest an explanation for substrate and site-specificity of RNA editing by APOBEC3A and -3G enzymes.

scholarly journals Prodrugs for Improved Drug Delivery: Lessons Learned from Recently Developed and Marketed Products

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1031
Author(s):  
Milica Markovic ◽  
Shimon Ben-Shabat ◽  
Arik Dahan
Keyword(s):  
Drug Therapy ◽  
Drug Effect ◽  
Parent Drug ◽  
Lessons Learned ◽  
The Body ◽  
Site Specificity ◽  
Site Specific ◽  
Specific Targeting ◽  
The Past ◽  
Optimal Drug

Prodrugs are bioreversible, inactive drug derivatives, which have the ability to convert into a parent drug in the body. In the past, prodrugs were used as a last option; however, nowadays, prodrugs are considered already in the early stages of drug development. Optimal prodrug needs to have effective absorption, distribution, metabolism, and elimination (ADME) features to be chemically stable, to be selective towards the particular site in the body, and to have appropriate safety. Traditional prodrug approach aims to improve physicochemical/biopharmaceutical drug properties; modern prodrugs also include cellular and molecular parameters to accomplish desired drug effect and site-specificity. Here, we present recently investigated prodrugs, their pharmaceutical and clinical advantages, and challenges facing the overall prodrug development. Given examples illustrate that prodrugs can accomplish appropriate solubility, increase permeability, provide site-specific targeting (i.e., to organs, tissues, enzymes, or transporters), overcome rapid drug metabolism, decrease toxicity, or provide better patient compliance, all with the aim to provide optimal drug therapy and outcome. Overall, the prodrug approach is a powerful tool to decrease the time/costs of developing new drug entities and improve overall drug therapy.

scholarly journals Stem-loop structure preference for site-specific RNA editing by APOBEC3A and APOBEC3G

2017 ◽  
Author(s):  
Shraddha Sharma ◽  
Bora E Baysal
Keyword(s):  
Nucleotide Sequence ◽  
Rna Editing ◽  
Site Preference ◽  
Site Specificity ◽  
Loop Structure ◽  
The Novel ◽  
Stem Loop ◽  
Site Specific ◽  
Cytidine Deaminases ◽  
Stem Loop Structure

APOBEC3A and APOBEC3G cytidine deaminases inhibit viruses and endogenous retrotransposons. We recently demonstrated the novel cellular C-to-U RNA editing function of APOBEC3A and APOBEC3G. Both enzymes deaminate single-stranded DNAs at multiple TC or CC nucleotide sequences, but edit only a select set of RNAs, often at a single TC or CC nucleotide sequence. To examine the specific site preference for APOBEC3A and -3G-mediated RNA editing, we performed mutagenesis studies of the endogenous cellular RNA substrates of both proteins. We demonstrate that both enzymes prefer RNA substrates that have a predicted stem-loop with the reactive C at the 3ʹ-end of the loop. The size of the loop, the nucleotides immediately 5ʹ to the target cytosine and stability of the stem have a major impact on the level of RNA editing. Our findings show that both sequence and secondary structure are preferred for RNA editing by APOBEC3A and -3G, and suggest an explanation for substrate and site-specificity of RNA editing by APOBEC3A and -3G enzymes.

scholarly journals Theatrum Mundi and site in four television Shakespeare films

2019 ◽  
Vol 99 (1) ◽  
pp. 76-88
Author(s):  
Victor Huertas Martín
Keyword(s):  
Julius Caesar ◽  
Site Specificity ◽  
Recent Criticism ◽  
Site Specific ◽  
Television Film

This article explores metatheatricality and site specificity in four Shakespeare television films produced by Illuminations Media: Gregory Doran’s Macbeth (2001), Hamlet (2009) and Julius Caesar (2012), and Rupert Goold’s Macbeth (2010). Drawing on metatheatrical theory applied to the screen and recent criticism on site-specific theatre, I explore the films as self-referential and self-conscious works embedded in environments that oppose the artifice of drama to the ‘reality’ of normative television film. Shakespeare’s aesthetic metaphor, presented in self-contained theatrical worlds, does not depict autonomous fictions but is disrupted by outside ‘reality’.

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