Prevalence of human papilloma virus (HPV) and type distribution of genotypes (6, 11, 16 and 18) among Egyptian women

2020 ◽  
Vol EJMM29 (4) ◽  
pp. 45-50
Author(s):  
Sahar A. Ibrahim ◽  
Shimaa A. Abdel-Salam ◽  
Mohamed I. Amer ◽  
Abeer A. El-Sayed ◽  
Rania A. Hassan
Keyword(s):  
Reproductive Tract ◽  
Hpv Infection ◽  
Dna Testing ◽  
Papilloma Virus ◽  
Type Distribution ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Hpv Prevalence ◽  
Egyptian Women ◽  
Polymerase Chain

Background: Human papillomavirus (HPV) is the most common viral infection of the reproductive tract and the causative agent of cervical cancer and genital warts. Objectives: to study prevalence of HPV infection and type distribution of genotypes (6, 11, 16 and 18) among Egyptian females. Methodology: 65 Egyptian women were subjected to conventional Pap cytology, HPV DNA testing by polymerase chain reaction (PCR) and genotyping by multiplex PCR for genotypes (6, 11, 16 and 18) during the period from May 2018 until October 2018. Results: The prevalence of HPV among participants was (23.1%). only 20% of HPV positive cases, were infected by single HPV genotype and 80% were co-infected by more than one genotype. Conclusion: These data expand the knowledge concerning HPV prevalence and type distribution in Egypt which may help to create a national HPV prevention program.

scholarly journals Negative Colposcopic Biopsy After Positive Human Papilloma Virus (HPV) DNA Testing

2006 ◽  
Vol 125 (3) ◽  
pp. 413-418 ◽  
Author(s):  
Amy L. Adams ◽  
Isam Eltoum ◽  
Janie Roberson ◽  
Jun Chen ◽  
Kathy Connolly ◽  
...  
Keyword(s):  
Human Papilloma Virus ◽  
Dna Testing ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Hpv Dna Testing

Human Papillomavirus DNA Testing as an Adjunct to Cytology in Cervical Screening Programs

2003 ◽  
Vol 127 (8) ◽  
pp. 959-968 ◽  
Author(s):  
Attila T. Lörincz ◽  
Ralph M. Richart
Keyword(s):  
High Grade ◽  
Dna Testing ◽  
Papanicolaou Test ◽  
Chain Reaction ◽  
Hpv Dna ◽  
Screening Programs ◽  
Hpv Dna Testing ◽  
Hybrid Capture 2 ◽  
Screening Intervals ◽  
Polymerase Chain

Abstract Our objective was to review current large studies of human papillomavirus (HPV) DNA testing as an adjunct to the Papanicolaou test for cervical cancer screening programs. We analyzed 10 large screening studies that used the Hybrid Capture 2 test and 3 studies that used the polymerase chain reaction test in a manner that enabled reliable estimates of accuracy for detecting or predicting high-grade cervical intraepithelial neoplasia (CIN). Most studies allowed comparison of HPV DNA and Papanicolaou testing and estimates of the performance of Papanicolaou and HPV DNA as combined tests. The studies were selected on the basis of a sufficient number of cases of high-grade CIN and cancer to provide meaningful statistical values. Investigators had to demonstrate the ability to generate reasonably reliable Hybrid Capture 2 or polymerase chain reaction data that were either minimally biased by nature of study design or that permitted analytical techniques for addressing issues of study bias to be applied. Studies had to provide data for the calculation of test sensitivity, specificity, predictive values, odds ratios, relative risks, confidence intervals, and other relevant measures. Final data were abstracted directly from published articles or estimated from descriptive statistics presented in the articles. In some studies, new analyses were performed from raw data supplied by the principal investigators. We concluded that HPV DNA testing was a more sensitive indicator for prevalent high-grade CIN than either conventional or liquid cytology. A combination of HPV DNA and Papanicolaou testing had almost 100% sensitivity and negative predictive value. The specificity of the combined tests was slightly lower than the specificity of the Papanicolaou test alone, but this decrease could potentially be offset by greater protection from neoplastic progression and cost savings available from extended screening intervals. One “double-negative” HPV DNA and Papanicolaou test indicated better prognostic assurance against risk of future CIN 3 than 3 subsequent negative conventional Papanicolaou tests and may safely allow 3-year screening intervals for such low-risk women.

scholarly journals Long-Term Follow-up of HPV Infection Using Urine and Cervical Quantitative HPV DNA Testing

2016 ◽  
Vol 17 (5) ◽  
pp. 750 ◽  
Author(s):  
Alex Vorsters ◽  
Severien Van Keer ◽  
Samantha Biesmans ◽  
Annick Hens ◽  
Ilse De Coster ◽  
...  
Keyword(s):  
Hpv Infection ◽  
Dna Testing ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Long Term Follow Up

scholarly journals Modern concepts of epidemiologyHPV-infection

2013 ◽  
Vol 62 (5) ◽  
pp. 3-14
Author(s):  
Kseniya Yevgenyevna Semenova ◽  
Armine Robertovna Khachaturyan
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Age Groups ◽  
Treatment Strategies ◽  
Hpv Infection ◽  
Dna Testing ◽  
Transmitted Infection ◽  
Hpv Dna ◽  
Sexually Transmitted ◽  
Hpv Dna Testing

Human papillomavirus (HPV) is widespread sexually transmitted infection, which is defined by high risk of contagion and high cancer potential. HPV extension depends on patiens age, residence, opportunites of diagnostics, etc. There are general methods of HPV-diagnostics such as HPV DNA testing, cytological, hystological methods, which are not always useful in detection of transitory and persistent HPV-infection forms. Nowadays, there is acute need to develop new diagnostic and prognostic methods, treatment strategies for patients from various age groups, having different infection formss.

scholarly journals Prevalence of human papillomavirus genotype among Moroccan women during a local screening program

2010 ◽  
Vol 4 (11) ◽  
pp. 732-739 ◽  
Author(s):  
Zaitouna Alhamany ◽  
Mohammed El Mzibri ◽  
Aicha Kharbach ◽  
Abderrahman Malihy ◽  
Redouane Abouqal ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Hpv Infection ◽  
Dna Testing ◽  
Cervical Lesions ◽  
Abnormal Cytology ◽  
Hpv 16 ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Moroccan Women

Introduction: Many studies have indicated a causal relationship between genital human papillomavirus (HPV) infections and cervical cancer. This study aimed to determine the prevalence and genotypes of six high-risk oncogenic human papillomaviruses in cervical lesions from Moroccan women with normal and abnormal cytology. Methodology: The study included 938 women from the Children's and Mothers' Pathology Department of Ibn Sina Hospital, Rabat. Cytopathology examination was done by routine PAP smear testing. HPV DNA testing was conducted using DNA amplification by Polymerase Chain Reaction with subsequent typing by hybridization with specific probes for HPV types 16, 18, 31, 33, 35 and 45. Results: Cytopathology testing showed that only 16.3 % had an abnormal cytology, with a predominance of atypical squamous cell of undetermined significance (ASCUS) cases. The overall HPV prevalence was 15.7%. According to the cytology results, HPV infection was detected in 15.8% of normal and 14.38% of abnormal cases. Specific HPV genotyping showed a predominance of HPV 16 and 18. Double infection (HPV 16 + 18) was found in two cases whereas multiple infections (HPV 16+18+31) were detected in only one case. Evaluation of the relationship between HPV status and some environmental risk factors, including individual, socio-economic, and hygiene status, showed a significant association between HPV infection and oral contraceptive use. Conclusion: Based on these data, a combination of cytology and HPV DNA testing allows for identification of patients with a high risk of developing high-grade cervical lesions and improves cervical cancer prevention.

scholarly journals Determination of papilloma-virus infection reproductive phase in algorithm of monitoring patients with cervical displasia

2021 ◽  
Vol 51 (4) ◽  
pp. 61-63
Author(s):  
S. A. Selkov ◽  
G. N. Vedeneeva ◽  
I. A. Baskakova ◽  
S. R. Baur
Keyword(s):  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Hpv 16 ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Virus Activity ◽  
Polymerase Chain

HPV 16 and 18 are known to be the main cause of cervical intraepithelial neoplasia (CIN) and cervical cancer. The terms of HPV persistence in the host and, coordinately, the risk of cervical neoplasia development and progression are determined in much extent by virus activity. The purpose of this investigation was the detection of HPV DNA presence in cervical epithelium as well as confirmation of its activity by means of immunocytochemistry and reverse transcriptase polymerase chain reaction. The level of HPV inf ection by oncogenic and nononcogenic types in 181 women with different cervical pathology was 55,8%. The active stage of HPV infection was confirmed in 27,5% of HPV-inf ected women mainly with low grades of CIN. The proof of reproductive general HPV infection was more informative with RT PCR just as for HPV 16 and 18 immunocytochemistry and RT PCR completed each anothe.

scholarly journals HPV DNA testing with cytology triage in cervical cancer screening: Influence of revealing HPV infection status

2015 ◽  
Vol 123 (12) ◽  
pp. 745-754 ◽  
Author(s):  
Lyndsay Ann Richardson ◽  
Mariam El-Zein ◽  
Agnihotram V. Ramanakumar ◽  
Samuel Ratnam ◽  
Ghislain Sangwa-Lugoma ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Screening ◽  
Cervical Cancer Screening ◽  
Hpv Infection ◽  
Infection Status ◽  
Dna Testing ◽  
Hpv Dna ◽  
Hpv Dna Testing

scholarly journals Association of serum 25-hydroxyvitamin D with prevalence, incidence, and clearance of vaginal HPV infection in young women

2020 ◽  
Author(s):  
Mariam El-Zein ◽  
Farzin Khosrow-Khavar ◽  
Ann N Burchell ◽  
Pierre-Paul Tellier ◽  
Shaun Eintracht ◽  
...  
Keyword(s):  
Vitamin D ◽  
Proportional Hazards ◽  
Hpv Infection ◽  
Cox Proportional Hazards ◽  
Baseline Serum ◽  
Hpv Dna ◽  
Hydroxyvitamin D ◽  
Hpv Prevalence ◽  
Vitamin D Levels ◽  
25 Hydroxyvitamin D

Abstract Background We assessed the association between serum 25-hydroxyvitamin D levels and genital human papillomavirus (HPV) prevalence, incidence, and clearance among female participants of the HITCH cohort study. Methods We genotyped HPV DNA in vaginal samples and quantified baseline serum 25-hydroxyvitamin D levels using Roche’s Linear Array and Total vitamin D assay, respectively. We used logistic and Cox proportional hazards models to respectively estimate adjusted odds ratios (OR) and hazards ratios (HR) with 95% confidence intervals (CI). Results There was no association between vitamin D levels (every 10ng/mL increase) at baseline and HPV prevalence (OR=0.88, CI:0.73-1.03) or incidence (HR=0.88, CI:0.73-1.06), but we observed a modest negative association with HPV clearance (HR=0.76, CI:0.60-0.96). Vitamin D levels <30ng/mL, compared to ≥30ng/mL, were not associated with HPV prevalence (OR=0.98, CI:0.57-1.69) or incidence (HR=0.87, CI:0.50-1.43), but were associated with a marginally significant increased clearance (OR=2.14, CI:0.99-4.64). We observed consistent results with restricted cubic spline modelling of vitamin D levels and clinically defined categories. HPV type-specific analyses accounting for multiple HPV infections per participant showed no association between vitamin D levels and all study outcomes. Conclusion This study provided no evidence of an association between low vitamin D levels and increased HPV prevalence, acquisition, or clearance.

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