In this article, the position control problem of electro-hydraulic servo systems with feedback signal transmission delay is studied. In order to improve the control accuracy of the system, a hybrid controller which combines a delay observer, a nonlinear disturbance observer and a backstepping controller is proposed. The controller has the characteristics of compensating the delay of signal transmission, restraining the uncertain disturbance of control systems and high control precision. In order to verify the stability and validity of the proposed hybrid controller, a single-degree-of-freedom electro-hydraulic shaking table is used to verify the experimental results. The experimental results show that the proposed controller has better control effects than proportional integral derivative and backstepping controller.