scholarly journals PRELIMINARY OBSERVATIONS ON THE SEMINAL CHARACTERISTICS OF RABBITS RAISED IN TWO LOCATIONS IN SOUTHEASTERN NIGERIA.

2021 ◽  
Vol 22 (2) ◽  
pp. 108-112
Author(s):  
U. Herbert ◽  
C. O. Acha
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Southeastern Nigeria ◽  
Abnormal Sperm ◽  
Port Harcourt ◽  
Semen Volume ◽  
Live Sperm ◽  
Artificial Vagina ◽  
Time Of Collection

A total of sixteen rabbits sourced from two locations in Southeastern Nigeria were used for this study. Semen was collected from all the rabbits twice weekly for eight weeks using an artificial vagina while observation on the effect of time of collection on semen quality were also made. The results obtained indicated that time of collection had no significant effect (P > 0.05) on all the seminal characteristics measured. However, source of the animals showed significant effect (P < 0.05) on semen volume, sperm concentration, total sperm per ejaculate and proportion of abnormal sperm. Average semen volume, sperm concentration and total sperm per ejaculate were: 0.69±0.007 ml, 104.0±1.44 x 106/m1 and 71.40±0.90x106 respectively for the rabbits sourced from Port Harcourt. The corresponding values for rabbits sourced from Owerri were: 0.66±0.008m1, 96.02 ±1.63x106iml and 63.49±1.50 x 106 respectively. Abnormal sperm proportions were 11.05±0.22% and 1216 ± 032% for the rabbits sourced from Port Harcourt and Owerri respectively. There were no significant (P> 0.05) differences observed between locations in live sperm concentration and sperm motility. Live sperm concentration and sperm motility were 87.75±1.09% and 71.62±0.74% for the Port Harcourt group, while the values obtained for the Owerri group were 88.06±034% and 71.19±0.89% respectively.  

scholarly journals Preservation of liquid semen and Artificial Insemination in native sheep

1970 ◽  
Vol 7 (2) ◽  
pp. 305-308 ◽  
Author(s):  
S Pervage ◽  
MR Hassan ◽  
M Ershaduzzaman ◽  
MAMY Khandoker
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Conception Rate ◽  
Abnormal Sperm ◽  
Semen Volume ◽  
Native Sheep ◽  
Artificial Vagina

An experiment was undertaken to determine the conception rate of native sheep by using Artificial Insemination with liquid ram semen. The semen was collected from ram using artificial vagina and the was stored in a refrigerator (4°C) for three days. The volume of semen was extended with egg yolk citrate diluter. A total of 63 ewes were inseminated with stored liquid semen collected from 15 rams by AV method. The total number of spermatozoa, live-dead, normal-abnormal, sperm motility and the pH was observed regularly. The average semen volume per ejaculate was 0.76-1.00ml and the sperm concentration was 2.37x109 - 4.30x109 per ejaculate. The number of normal spermatozoa and the pH was almost similar irrespective of days of storage. Number of live spermatozoa and the sperm motility were reduced with the increasing age of semen. The average conception rate (%) was obtained as 63.61, 61.90, 52.38 and 47.61 with sperm in zero, 1st, 2nd and 3rd day storage respectively. Keywords: Preservation; Liquid semen; Quality; Artificial insemination; Sheep DOI: 10.3329/jbau.v7i2.4739 J. Bangladesh Agril. Univ. 7(2): 305-308, 2009

scholarly journals Raw Semen Characteristics of Three Different Indonesian Local Roosters

2016 ◽  
Vol 18 (3) ◽  
pp. 165
Author(s):  
Emilia Kamung Hambu ◽  
Raden Iis Arifiantini ◽  
Bambang Purwantara ◽  
Sri Darwati
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Mass Movement ◽  
Sperm Concentration ◽  
Semen Volume ◽  
Sperm Abnormality ◽  
Microscopic Evaluation ◽  
Semen Characteristics ◽  
Live Sperm ◽  
Better Than

Indonesia has agreat variety of roosters, either indigenous type as well as exotic and cross breed. The purpose of this experiment was to study the characteristics of semen from three types of Indonesian local roosters such as Merawang, Kampung and crosses Sentul Kampung with Kedu (SK Kedu). A total of 15 roosters consist of  Merawang roosters,  Kampung, and SK Kedu roosters were 5 each. The semen was collected 3 times a week by dorso-abdominal and cloaca massage method. The parameters evaluation was macroscopic characteristics consist of volume, color, consistency, and pH. Microscopic evaluation of semen such as a mass movement, sperm motility, live sperm, sperm abnormality and sperm concentration. Results of this experiment showed that semen volume of  Merawang  (0.40±0.26 mL) was higher (p<0.05) compare to Kampung (0.24±0.12 mL) or  SK Kedu (0.16±0.10 mL) but no difference on semen color, consistency and semen pH. There were no difference in the mass movement, sperm motility and live sperm as well as on sperm abnormality among three types of roosters.  Sperm  concentration of Merawang (4490 million mL-1) was significantly higher than Kampung (3245 million mL-1) and the SK Kedu roosters (3751 million mL-1). Its was conclude that Merawang roosters had good semen quality better than Kampung and SK Kedu roosters

scholarly journals Cryopreservation and quality assessment of boar semen collected from bulk samples

2019 ◽  
Vol 64 (No. 5) ◽  
pp. 209-216 ◽  
Author(s):  
R Ratchamak ◽  
T Vongpralub ◽  
W Boonkum ◽  
V Chankitisakul
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Bulk Sample ◽  
Energy Status ◽  
Semen Volume ◽  
Boar Semen ◽  
Live Sperm ◽  
Fresh Semen

The purpose of this study was to examine sperm quality after cryopreservation of ejaculates collected as a bulk sample, which is routinely part of semen collection, and to compare this quality with the sperm-rich fraction in boars. Ejaculates were collected as sperm-rich fractions (SRF) and bulk samples (BE) using a gloved-hand technique. Fresh semen quality in terms of semen volume, sperm concentration, total sperm motility and pH were conventionally evaluated. Then, semen was cryopreserved using the liquid nitrogen vapour method. The post-thaw sperm quality was evaluated by assessing sperm motility, live sperm with normal apical ridge and high mitochondrial energy status, lipid peroxidation was evaluated using CASA and fluorescent multiple staining and MDA levels were determined using a spectrophotometer, respectively. In terms of fresh semen quality, sperm motility in fresh semen did not differ significantly between the two groups. The treatment with the greater mean volume (BE; P &lt; 0.05) had a lower mean sperm concentration (P &lt; 0.05); meanwhile, the mean ejaculate pH collected as BE was more basic compared with SRF (P &lt; 0.05). However, there were no significant post-thaw quality changes between sperm-rich fractions and bulk samples of semen. In conclusion, ejaculates can be collected as bulk samples without the need to classify fractions for boar semen cryopreservation.

scholarly journals Study on buck evaluation based on semen quality and fertility

2013 ◽  
Vol 42 (2) ◽  
pp. 101-108 ◽  
Author(s):  
F Sultana ◽  
SS Husain ◽  
A Khatun ◽  
AS Apu ◽  
MAMY Khandoker
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Normal Sperm ◽  
Semen Volume ◽  
Fertility Data ◽  
Three Stages ◽  
Live Sperm

The experiment was conducted to evaluate the performance of buck on the basis of semen quality and fertility. Data on 162 ejaculates from five adult Black Bengal bucks (B1, B2, B3, B4 and B5) were recorded. Individual buck effect was found to be significant on semen volume (p<0.05), three stages of sperm motility (p<0.01) and percentage of live sperm (p<0.01) but insignificant (p>0.05) on sperm concentration and percentage of normal sperm. The results of semen volume per ejaculate, sperm concentration, initial sperm motility, on dilution sperm motility, post-thawing sperm motility, percentage of live sperm and percentage of normal sperm were ranged from 0.58±0.17 to 1.04±1.1 ml, 2827±0.76 to 3132

Sexual Behavior and its Relationship with Semen QualityParameters in Jaffrabadi Breeding Bulls

2019 ◽  
Vol 15 (01) ◽  
pp. 57-60
Author(s):  
J K Chaudhary ◽  
G B Solanki ◽  
H P Vijyeta ◽  
P M Gamit ◽  
K S Murthy
Keyword(s):  
Sexual Behavior ◽  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Mating Ability ◽  
Mean Values ◽  
Semen Volume ◽  
Mass Activity ◽  
Live Sperm

The present study was carried out on 80 ejaculates, 20 each from four healthy Jaffrabadi breeding bulls to see the relationship betweensexual behavior and semen quality parameters. The results depicted that the overall mean values of libido score, mating ability score andsexual libido score between bulls. The overall color of semen ranged from thin white to very thick creamy. The overall values for semen volume(ml), pH, sperm concentration (million/mL), mass activity, individual sperm motility (%) and live spermatozoa (%) were 2.99 ± 0.12, 6.80 ± 0.01, 1439.68 ± 48.46, 3.94 ± 0.03, 89.51 ± 0.21 and 95.00 ± 0.19, respectively. The values for semen volume, sperm concentration,mass activity (%) and live sperm (%) differed significantly (p less than 0.05) between bulls. There were significant positive correlations (p LESS THAN 0.01)of libido with mating ability, sexual behavior score and ejaculate volume of semen (r = 0.37 to 0.87), while a negative correlation wasfound with sperm concentration (-0.30). Mating ability was significantly (p less than 0.01) correlated with sexual behavior score and ejaculatevolume (0.78, 0.67), and sexual behavior was correlated with semen volume (0.84). Sperm concentration was significantly (p less than 0.05 correlated with mass activity (0.29), while individual sperm motility was positively (p less than 0.05) correlated with live sperm percentage (0.25).Correlations of libido, reflected the importance of sexual behavior of Jaffrabadi bulls in predicting their future utility as a proficient breeder under AI program.

scholarly journals Effect of Age and Frequency of Collection on Quality of Jersey Bulls Semen at National Livestock Breeding Center (NLBC), Nepal

2019 ◽  
Vol 7 (1) ◽  
pp. 88-95 ◽  
Author(s):  
S. Sankhi ◽  
K.R. Sapkota ◽  
B. Regmi
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Bull Semen ◽  
Livestock Breeding ◽  
Abnormal Sperm ◽  
Breeding Center ◽  
Live Sperm ◽  
Level Of Significance ◽  
Effect Of Age

Objective: To determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara. Materials and Methodology: Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3-4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan, 2016). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0 Results: Sperm concentration, post- thaw motility, live sperm, and abnormal sperm were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. Significantly higher value for semen volume, sperm concentration and post- thaw motility was obtained on second collection (P<0.05). However, volume initial motility, pre-filling motility, live sperm and abnormal sperm percentage did not differ significantly among different age intervals and between the frequencies of collection. Conclusion: Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentages were higher in 5-7 years old bulls and in second collection of semen. Int. J. Appl. Sci. Biotechnol. Vol 7(1): 88-95

scholarly journals Effect of Age and Frequency of Collection in Quality of Jersey Bulls Semen at National Livestock Breeding Center, Nepal

2018 ◽  
Vol 35 ◽  
pp. 9-22
Author(s):  
S. Sankhi ◽  
K.R. Sapkota ◽  
B. Regmi
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Bull Semen ◽  
Livestock Breeding ◽  
Abnormal Sperm ◽  
Breeding Center ◽  
Live Sperm ◽  
Level Of Significance ◽  
Effect Of Age

The present study was undertaken to determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara, Kaski district. Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3- 4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan 2017). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0. Sperm concentration (0.551 ±0.004 to 1.084 ± 0.014x109), post- thaw motility (42.44±0.11 to 51.72±0.03 %), live sperm (45.62 ± 0.13 to 51.23±0.15 %), and abnormal sperm (11.57±0.12 to 19.59±0.24%) were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. On the other hand, volume (3.98±0.09 to 6.72±0.06 ml), initial motility (72.68±0.02 to 78.64±0.15 %) and pre-filling motility (71.34±0.17 to 77.62±0.02 %) did not differ significantly among different age intervals. Significantly, higher value for semen volume (3.98±0.12 to 6.02±0.14 ml), sperm concentration (0.572±0.119 to 1.095±0.015) and post- thaw motility (40.34±0.21 to 48.54±0.06% was obtained on second collection. However, initial motility (70.58±0.06 to 75.64±0.13 %), prefilling motility (68.11±0.12 to 71.62±0.02 %), live sperm (53.27±0.54 to 56.17±0.02 %) and abnormal sperm (12.31±0.12 to 14.3±0.47 %) did not differ significantly between the frequencies of collection. Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentage were higher in 5-7 years old bulls and in second collection of semen.

scholarly journals Repeatability Estimates For Seminal Traits And Their Phenotypic Relationships With Testes Measurements And Performance Traits In Black Bengal Buck

1970 ◽  
Vol 37 (2) ◽  
pp. 34-41 ◽  
Author(s):  
AK Rajuana ◽  
MR Tayabur ◽  
MA Hoque ◽  
SS Husain ◽  
Z Sultana
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Body Condition ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Normal Sperm ◽  
Semen Volume ◽  
Performance Traits ◽  
Mass Activity ◽  
And Performance

Repeatability for seminal traits and their phenotypic relationships with testes measurements and performance traits in Black Bengal bucks were estimated from a total of 116 repeated observations on 15 young Black Bengal bucks. Performance traits included age, body condition and body weight of bucks. Testes measurements were testes length, breadth and volume, and scrotal circumference, while seminal traits were ejaculate volume, semen density, mass activity, sperm motility, sperm concentration, total sperm per ejaculation and percent of normal sperm. High positive correlations (ranging from 0.81 to 0.90) were found between body weight and testes measurements. Semen volume and percent of normal sperm were positively correlated with age, body condition and body weight of bucks (ranging from 0.24 to 0.60). The testes measurements were strongly and positively correlated with semen volume and total sperm per ejaculation (ranging from 0.53 to 0.61), while the correlations between testes measurement and percent of normal sperm were moderate (ranging from 0.34 to 0.44). Among seminal traits, strong correlations were found between semen volume and total sperm per ejaculation (0.81) and, between mass activity and sperm motility (0.82). Repeatability of ejaculate volume was higher (r = 0.78) which indicated that selection or culling for semen ejaculate volume could be practiced from single or few observations. It could be concluded that bucks’ age, body weight and body condition along with testes volume should be considered as selection criteria for improving semen quality and semen production of breeding bucks. DOI: http://dx.doi.org/10.3329/bjas.v37i2.9879 BJAS 2008; 37(2): 34-41

116. EFFECTS OF SUPPLEMENTAL VITAMIN ON THE SEMEN CHARACTERISTICS OF MARKHOZ GOATS DURING NON-BREEDING SEASON

2009 ◽  
Vol 21 (9) ◽  
pp. 35
Author(s):  
A. Towhidi ◽  
A. Farshad ◽  
M. Dolatpanah ◽  
R. Salehi
Keyword(s):  
Vitamin E ◽  
Breeding Season ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Progressive Motility ◽  
Semen Volume ◽  
Semen Characteristics ◽  
Supplemental Vitamin ◽  
Diet Control ◽  
Live Sperm

The aim of the study was to investigate the effect of dietary supplementation of vitamin E on the semen characteristics of the Markhoz goats. Eighth bucks were randomly allocated into two groups, and received two different diets: unsupplemented diet (control) and supplemented diet with vitamin E (0.30 g/Kg DM). All experimental diets were formulated according to AFRC 1998. Semen was collected at 14-days intervals from June 17, 2006 to September 2, 2006 (non-breeding season) using artificial vagina. Semen characteristics were evaluated including semen volume, sperm concentration, live sperm percentage, percentage of motility and progressive motility. This characteristics were evaluated at two weeks intervals the trial. The total number of spermatozoa per ejaculation was calculated by multiplication of the semen volume with sperm concentration. Sperm motility was also analyzed by placing a sample on pre-warmed (37 º C) microscope slide covered with a cover slip, and examined under a high power microscope at a magnification × 200. Data was analyzed using proc MIXED in SAS program. Significant effect (P≤0.05) of the week (sampling time) was observed for all the parameters except for semen volume. Vitamin E supplementation significantly improved (P≤0.05) total number (Control: 263.7 ×107±17.506 vs. Vit E: 320.95 ×107±17.506) and sperm concentration (Control: 301.79 ×107±13.657 vs. Vit E: 386.57 ×107± 13.657), motility (Control: 77.27% ±0.89 vs. Vit E: 82.6% ±0.89) and progressive motility (Control: 4.208 ±0.138 vs. Vit E: 4.229 ±0.138), percentage of viability (Control: 80.57% ±0.89 vs. Vit E: 85.9% ±0.89). The results suggested that the supplemental Vitamin E may improve the semen quality and fertility in the Markhoz goats.

scholarly journals Breeding soundness of stud bulls

1970 ◽  
Vol 25 (2) ◽  
pp. 51-61 ◽  
Author(s):  
SP Shaha ◽  
MGS Alam ◽  
M Khatun ◽  
JU Ahmed
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Holstein Friesian ◽  
Semen Volume ◽  
Breeding Soundness

Four bulls of mixed breeds (Sahiwal × Zebu, Sindhi × Zebu, Jersey × Zebu and Holstein-Friesian × Zebu )were examined physically and their semen was evaluated in three seasons: I (Rainy): May - November, II (Winter): December - February, III (Summer): March- April. The semen volume, sperm motility, sperm concentration and percentage of dead sperm ranged from 4.1 to 7.6 ml, 56.6 to 76%, 1010.3 to 1290.7 × 106/ml and 18.4 to 24.7%, respectively. All these attributes varied significantly (p<0.05) between breeds and seasons. The variations of semen quality between breeds were not significant (p>0.05). It is suggested that the Holstein - Friesian × Zebu crossbred bull produces better quality semen than others. DOI: 10.3329/bvet.v25i2.4618 Bangl. vet. 2008. Vol. 25, No. 2, 51-61

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