scholarly journals Haematology and serum profile of rabbits due to generation interval, housing systems and sex

2020 ◽  
Vol 42 (1) ◽  
Author(s):  
O. Y. Ayo-Ajasa ◽  
A. B. J. Aina ◽  
O. S. Sowande ◽  
L. T. Egbeyale ◽  
M. O. Ozoje ◽  
...  

A total of ninety-six (96) weaner rabbits (Chinchilla and New Zealand White crossbred) were use for this study and were divided into forty-eight (48) per generation. These fortyeight weaner rabbits were further divided into three replicates of four males (12) and four females each (12) housed in cage (24) and deep litter system (24).This experiment was carried out for two generations to determine the effect of generation interval, housing systems and sex on the haematological and serum profile of rabbits. Data obtained were analyzed using Analysis of Variance test in a 2x2x2 factorial arrangement. The study showed that Packed cell volume (PCV), red blood cells (RBC) and haemoglobin (HB) were not significant in both generations while higher significant(p<0.05) values of albumin were observed in first generation and white blood cells (WBC), globulin, aspartate amino transferase (AST) and alanine amino transferase (ALT) were significantly higher in the second generation. Sex had no significant (p>0.05) effect on most of the biochemical parameters studied except for RBC and glucose which had 4.12 x1012/L and 69.23 mg/dl, for males and 3. 8 x1012/L and 78.78mg/dl for females, respectively in the second generation. In addition, the AST and ALT values were also higher in the females than in the males in the first generation but with no significant (p>0.05) difference. Housing system result showed that WBC (6.29 x109/L) and glucose (78.35 mg/dl) of rabbits were significantly (p<0.05) increased in the cage and deep litter system, respectively in the first generation while total serum protein, albumin and globulin were significantly (p<0.05) higher in rabbits housed in the deep litter system in the second generation. Higher values were recorded for AST and ALT in the deep litter system than the cage system in both generations except that the values of ALT were within the same range for both housing systems in the second generation. This study clearly indicated that both housing systems support rabbit production without any negative influence on the immune status of the rabbit.

Author(s):  
Roy Tushar Kanti ◽  
Saha Samir Kumar

In present study an attempt has been made to investigate the combined impact of sub lethal toxicity of Cypermethrin 25% EC and pH on certain blood parameters of Puntius javanicus. Adult fish were exposed to sublethal concentration (0.36 µg/L) of cypermethrin 25% EC in different pH water i.e. pH 5, pH 6, pH 7, pH 8.5 and pH 9.5 for 15 days. Significant changes in red blood cell (RBCs) count, total white blood cells (WBCs), haemoglobin % (Hb%), haematocrit value, total serum protein and blood glucose level were observed in fish exposed to sub-lethal concentration of cypermethrin. Significant decrease in red blood cell (RBCs) count, haemoglobin % (Hb %), haematocrit value and total serum protein content were found in fish exposed to sub-lethal concentration of cypermethrin in pH 5, pH 6 and pH 9.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. A significant increase in these parameters were documented in fish exposed to sub-lethal concentration of cypermethrin in pH 8.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. On the other hand a significant increase of total white blood cells (WBCs) and blood glucose level were recorded in fish subjected to sub-lethal concentration of cypermethrin in pH 5, pH 6 and pH 9.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. Values of these parameters were decreased significantly in fish exposed to sub-lethal concentration of cypermethrin in pH 8.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. The values of all studied blood parameters in fish subjected to sub-lethal concentration of cypermethrin in pH 8.5 were almost found to retain the values of control group (pH 7, without toxicant).


2009 ◽  
Vol 54 (No. 4) ◽  
pp. 175-182 ◽  
Author(s):  
K. Satue ◽  
O. Blanco ◽  
A. Munoz

Normal hematological values need to be defined for each equine breed and age in order to increase diagnostic precision. No published data on hematology exist for Carthusian horses. This research compares the hematological characteristics of pregnant Carthusian broodmares of different ages. Forty-four pregnant Carthusian broodmares were divided into three age Groups: A (4–7 years; <I>n</I> = 18), B (8–12; <I>n</I> = 15) and C (13–17; <I>n</I> = 11). Jugular blood samples were taken every 14 days during pregnancy and data were pooled for each animal. The following hematological variables were determined: red blood cells (RBC), hemoglobin (HB), hematocrit (HCT), volumetric indices, white blood cells (WBC) and platelets (PLT). Furthermore, the numbers and percentages of lymphocytes (LYMP), band (BNL) and total neutrophils (NL), eosinophils (EOS), monocytes (MON), basophils (BAS) and the neutrophil/lymphocyte ratio (N/L) were counted on blood smears. Total serum protein concentrations (TSP) were also measured. The lower values of RBC, WBC, LYMP and PLT in the older broodmares (Group C) possibly reflected a decline in bone marrow activity. The lower RBC of these mares was compensated by an increased MCV. The higher NL values in Group C, both BNL and NL, could have represented subclinical infections, since these animals also presented the highest TSP. Likewise, the animals of Group C showed the highest EOS counts. This research demonstrated that ageing significantly influences the hematological values of Carthusian broodmares, with the most marked differences in mares older than 13 years and that these physiological variations must be taken into account in a clinical context.


2000 ◽  
Vol 71 (3) ◽  
pp. 561-570 ◽  
Author(s):  
L. A. Boyle ◽  
F. C. Leonard ◽  
P. B. Lynch ◽  
P. Brophy

AbstractThere is some evidence to show that loose housing during gestation has a negative influence on the welfare of sows subsequently in farrowing crates. However, little is known about the effects of the gestation housing on the initial responses of gilts to the farrowing crate or of the effects on gilt welfare throughout lactation. The objective of this experiment was to evaluate three gestation housing systems (1) stalls (ST); (2) loose-bedded (LB) and (3) loose-unbedded (LU) pens for their effect on behaviour, heart rate and skin lesion scores of gilts in farrowing crates. During the 1st h in the farrowing crate, LB gilts were more active, while ST gilts were more vocal. Although heart rates during the 1st h did not differ significantly between treatments, LB and LU gilts had significantly higher mean heart rates during the first 5 min in the crate, compared with 35 min later. Posture changes during the first 24 h did not differ between treatments. However, there was a significant reduction in the number of posture changes made by ST gilts but not LB or LU gilts by day 8. Increases in the skin lesion score of gilts in all three treatments were observed after 24 h in the crate, further increases were observed post farrowing in both loose treatments. Although no significant differences in the skin lesion score were observed during lactation, LB gilts were weaned with lower lesion scores than LU or ST gilts. Gilts from both loose treatments experienced greater distress at first introduction to the farrowing crate. However, the change in environment also had an adverse affect on the welfare of ST gilts. Skin damage and continuing discomfort of the loose-housed gilts post farrowing suggests that they experienced more stress at parturition. Bedding during gestation had a beneficial effect on skin health in the farrowing crate that persisted until weaning.


1925 ◽  
Vol 21 (2) ◽  
pp. 204-204
Author(s):  
J. Daikhovsky

After examining the serum globulin content of several sick and healthy individuals while determining the sedimentation rate of red blood cells (SR), Salomon found that in cases with normal SR the amount of globulin in the serum was about 30% of the total serum protein content; in patients with moderately accelerated SR the globulin content ranged between 36% and 51%; finally in cases with significantly accelerated SR the globulin content ranged between 37% and 70%.


2018 ◽  
Vol 26 (5) ◽  
pp. 103-119
Author(s):  
Dakhel Ghani Omran AL-Watify

Burns are associated with increasing metabolic activities of the body organs. High metabolism rates are initiated by activation of different inflammatory reactions and cellular mediators( cytokines). The present research was conducted to evaluate the inflammatory markers , proteins , and white blood cells in thermal burned patients . A total number of burned patients was 60 from both sexes ( 30 males and 30 females), then, the patients were classified according to severity of burn into four subgroups , the first burned male group involved 15 burned males affected with second degree of burn, and the second burned male group(15) was affected with third degree of burn . Similarly, the same classification was applied on burned females in this study. Thirty healthy subjects (15 males, 15 females) were selected as control groups. All ages of patients and controls were ranged between 2 5-35 years old.   It was well found that the levels of C- reactive protein ( CRP) were markedly increased ( p < 0 . 05 ) in all patient groups in a comparison with their normal counterparts . Results of interleukin – 6 ( IL-6 ) were significantly heightened ( p< 0. 05) in most burned patient groups except male group affected with second degree of burn ,since, they did not show a significant increase ( p>0.0 5 ). Concentrations of total serum protein , albumin , and globulin were tend to decrease significantly ( p < 0 . 0 5 ) in most burned groups when compared with those healthy control groups.   Regarding total white blood cells (WBCs) count, data obtained from the present study indicated a significant increase (p<0.05) of total WBCs in all burned patient groups as matching with control groups. Furthermore, differential WBCs explained the follow-  ing abnormalities: lymphocyte levels were significantly decreased (p<0.05) in all patient groups. Inversely, values of monocytes indicated an insignificant elevation (p>0.05) in most burned groups except burned male group affected with third degree of burn which showed a significant increase (p<0.05) in comparison with healthy groups. Statistical analysis of t-test indicated that granulocytes pointed out a significant elevation (p<0.05) Neutrophils level in all studied groups. Inversely, values of eosinophils were signifi- cantly reduced (p<0.05) in most burned groups compared to healthy control groups. Sig- nificant and insignificant decreases were found in the levels of basophils of all burned groups when compared to those of control group. 


2020 ◽  
Vol 51 (5) ◽  
pp. 1357-1366
Author(s):  
Othman & Kakey

This study was aimed to determine the residual concentrations of pesticides used in greenhouse cucumber fields and their effects on the farmers that spray it. Sixteen active cucumber greenhouse fields with 96 people (75 sprayers and 21 volunteers as control) randomly had been selected. In all selected greenhouses, abamectin, thiamethoxam, pyridabin, and spirodiclofen pesticides were used, their cucumber with leaves and groundwater collected in December 2017, March, June, and September 2018 with workers blood samples, were immediately brought to the laboratory. Results showed the occurrence of residual pesticides in the plant and groundwater samples with a highest level in the plant samples, while decreased in water samples. That is in comparison with control, sprayer blood results showed that cholinesterase activity decreased significantly meanwhile, liver function enzymes: serum glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, alkaline phosphatase, direct and total serum bilirubin increased significantly. The kidney function test revealed that the mean serum urea concentration in the sprayers was considerably increased compared to the controls, while there was no significant change between the sprayers and the control serum creatinine. Slight variations in the declining number of red blood cells; however, white blood cells have significantly risen to their upper limit within normal ranges.


Author(s):  
John W Foote ◽  
Lesley J Hinks

The total, albumin-bound, and α2-macroglobulin-bound zinc concentrations in serum samples obtained from 20 haemodialysis patients have been measured. Blood leucocyte zinc contents were also determined. The total serum zinc concentrations in these samples were low, and in each case this was due to reduction in the zinc associated with albumin, although the concentrations of albumin itself were normal. There was no abnormality of the zinc bound to α2-macroglobulin. These data demonstrate a disturbance of the plasma transport of zinc in haemodialysis patients which might be due either to a reduced affinity of albumin for zinc in uraemic subjects, or the result of zinc deficiency. The zinc contents of white blood cells were also significantly reduced, supporting the possibility that these patients were indeed zinc deficient.


Author(s):  
Delma P. Thomas ◽  
Dianne E. Godar

Ultraviolet radiation (UVR) from all three waveband regions of the UV spectrum, UVA (320-400 nm), UVB (290-320 nm), and UVC (200-290 nm), can be emitted by some medical devices and consumer products. Sunlamps can expose the blood to a considerable amount of UVR, particularly UVA and/or UVB. The percent transmission of each waveband through the epidermis to the dermis, which contains blood, increases in the order of increasing wavelength: UVC (10%) < UVB (20%) < UVA (30%). To investigate the effects of UVR on white blood cells, we chose transmission electron microscopy to examine the ultrastructure changes in L5178Y-R murine lymphoma cells.


1990 ◽  
Vol 63 (01) ◽  
pp. 112-121 ◽  
Author(s):  
David N Bell ◽  
Samira Spain ◽  
Harry L Goldsmith

SummaryThe effect of red blood cells, rbc, and shear rate on the ADPinduced aggregation of platelets in whole blood, WB, flowing through polyethylene tubing was studied using a previously described technique (1). Effluent WB was collected into 0.5% glutaraldehyde and the red blood cells removed by centrifugation through Percoll. At 23°C the rate of single platelet aggregtion was upt to 9× greater in WB than previously found in platelet-rich plasma (2) at mean tube shear rates Ḡ = 41.9,335, and 1,920 s−1, and at both 0.2 and 1.0 µM ADP. At 0.2 pM ADP, the rate of aggregation was greatest at Ḡ = 41.9 s−1 over the first 1.7 s mean transit time through the flow tube, t, but decreased steadily with time. At Ḡ ≥335 s−1 the rate of aggregation increased between t = 1.7 and 8.6 s; however, aggregate size decreased with increasing shear rate. At 1.0 µM ADP, the initial rate of single platelet aggregation was still highest at Ḡ = 41.9 s1 where large aggregates up to several millimeters in diameter containing rbc formed by t = 43 s. At this ADP concentration, aggregate size was still limited at Ḡ ≥335 s−1 but the rate of single platelet aggregation was markedly greater than at 0.2 pM ADP. By t = 43 s, no single platelets remained and rbc were not incorporated into aggregates. Although aggregate size increased slowly, large aggregates eventually formed. White blood cells were not significantly incorporated into aggregates at any shear rate or ADP concentration. Since the present technique did not induce platelet thromboxane A2 formation or cause cell lysis, these experiments provide evidence for a purely mechanical effect of rbc in augmenting platelet aggregation in WB.


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