The Restrictive Type, Infectious Complications, a Long Duration of Illness Might Predict Nadir Hematological Values Among Individuals With Anorexia Nervosa During the Refeeding Period: A Retrospective Study

BackgroundAlthough hematological abnormalities in patients with anorexia nervosa have been documented, the mechanisms involved have not been fully clarified, especially during the refeeding period, when hematological values further decrease after admission prior to improving. Here we address potential mechanisms underlying the hematological abnormalities of anorexic inpatients during the refeeding period.MethodsWe recruited patients from 101 admissions corresponding to 55 individual patients with anorexia nervosa with severe malnutrition (body mass index, 13.4 ± 3.4) from the neuropsychiatry unit in Ashikaga Red Cross Hospital during the period from October 1999 to March 2018. We analyzed three hematological cell measures (hemoglobin, white blood cells, platelets) to determine their levels at admission and their lowest levels during the refeeding period and calculated the rate of decrease in those values from admission to the nadir levels. We analyzed each measure using a general mixed model with explanatory variables, including data upon admission and a treatment-related indicator. ResultsThe initial hemoglobin value of 12.1 ± 2.7 g/dl decreased by 22.3% to 9.4 ± 2.5 g/dl; the initial white cell count was 5387 ± 3474/μl, which decreased by 33.6% to 3576 ± 1440/μl; the initial platelet count of 22.6 ± 10.1 ×104/μl decreased by 24.3% to 17.1 ± 8.0 ×104/μl. All nadir levels were observed during the refeeding period from the fifth to tenth day of hospitalization. The anorexia nervosa restrictive type was associated with a lower nadir level of hemoglobin and white blood cell count. Infectious complications were related to a lower nadir level of hemoglobin and platelet count. A longer duration of illness was associated with a lower nadir white blood cell count. ConclusionsNadir hematological cell measures of inpatients with anorexia nervosa during the refeeding period might be predicted by the restrictive type, infectious complications, and a long duration of illness. These factors might be used as indicators to guide clinical approaches for controlling hematological deficiencies during the refeeding period.

Assessing the Heamatology of Uda Rams Fed Graded Levels of Processed Cassava (Manihot esculentus L.) Peels

The experiment was carried out at Kebbi State University of Science and Technology, Aliero using thirty two (32) yearlings Uda Rams in two feeding trials ran concurrently. Sixteen (16) rams were used in each experiment with four treatments replicated four times in a completely randomized factorial design (2 × 4). The animal represents the replicates while the processing method (drying and ensiling) and the level of inclusion represents the treatments respectively. The level of inclusion are 0, 10, 20 and 30% dried cassava peels (DCP) and ensiled cassava peels (ECP) respectively. Data were collected in each trial on hematological characteristics. Data generated was subjected to analysis of variance and least significant difference (LSD) was used to separate the means. Hematological values of rams fed DCP were within the normal range while those fed ECP were below the normal range. The results shows significant difference (P<0.05) between dried and ensiled method of processing in terms of haemoglobin, MCH, WBC and MCV. Rams fed dried cassava peels had lower haemoglobin and PCV compared to normal range. It was concluded that there was no significant difference between rams fed dried cassava peels and those fed ensiled cassava peels at 30% level of inclusion.

P–430 Male fertility preservation: is there a role for cancer-induced inflammation that affects semen quality in oncological patients?

Study question What is the cause of semen quality impairment in oncological patients during fertility preservation programs? The cancer type and stadiation or the resulting inflammatory state? Summary answer The inflammatory state seems to be related to the decrease of sperm concentration, motility, morphology and viability due to the worsening of oxidative stress microenvironment. What is known already Fertility preservation acquired a great importance in the last decades due to increase survival of oncological patients, boost of diagnosis under 40 years and postponement of paternal age. At the time of cryopreservation, only one third of these males are normozoospermic. Tumor itself or other factors, added to psychological reasons, may be involved but there is no clear evidence. An imbalance of ROS (reactive oxygen species) in semen can compromise its quality. However, the correlation between cancer-related generalized stress state and fertility is poorly investigated. Inflammatory conditions induced by infections and pathologies, including cancer, increase ROS. Study design, size, duration Retrospective observational analysis was performed on 45 patients (29.0 ± 6.9 yrs) recruited during their fertility preservation program between 2016 and 2019 with written consent on use of their clinical data for research purpose. Patients presented several oncological diagnoses. Semen samples obtained from multiple collections (N = 58) were analyzed before applying standard freezing protocol. Data on semen parameters, inflammatory indices, hematological values and type/stage of tumors were collected. No exclusion criteria were applied. Participants/materials, setting, methods Routine semen analysis was performed according to the WHO standards. Sperm concentration and motility were evaluated on Makler Chamber, whereas eosin stain and Diff-quick slides were used for viability and morphology, respectively. Lymphoma was present in 72% of cases, leukemia in 8%, seminoma in 7% and other cancers in 13%. Correlations (Pearson/Spearman tests) among principal semen parameters and hematological values (leukocytes, erythrocytes, hemoglobin, RDW, albumin, etc.) were calculated with a P-value &lt;0.05 considered statistically significant. Main results and the role of chance The majority of semen samples showed a severe impairment, with one or more parameters under lower reference limits (WHO): 48.3% had sperm concentration under 15 millions/ml, 43.1% had a progressive motility under 32%, 41.4% had viability under 58% and 91.4% had abnormal morphology (under 4%). The role of potential inflammatory state was analyzed by correlating semen parameters and some hematological values. No correlation was found with cancer type. Negative association resulted between progressive motility (%PR) and leukocytes (p = 0.041) or RDW% (p = 0.015), but positive one with albumin (p = 0.012). Even sperm count, total motility (%PR+NP) and morphology were significantly correlated to RDW% (p = 0.003, p = 0.032, p = 0.034, respectively). These findings suggest a possible role of inflammation and ROS related generation in semen quality impairment. Indeed, albumin exerts a protective action, but leukocytes are known to cause ROS increase. Cancer-induced oxidative stress state may alter red blood cells homeostasis and vitality and increase erythrocytes turnover resulting in high RDW values. It is likely semen is worse when blood values indicate more severe cancer-induced inflammatory condition. Limitations, reasons for caution Significant correlations with type/stage of cancer were not found due to small number of each diagnosis, in spite our study considered 3 years of patients inclusion. Moreover, we lack to analyze the same patient before the cancer onset to avoid the influence of inflammatory state generated by the tumor itself. Wider implications of the findings: Understanding the influence of cancer-induced inflammatory state on semen quality could increase the awareness that clinicians should direct patient to the fertility preservation as soon as possible, even if diagnosis is still ongoing. It should be evaluated whether offering specific treatments may reduce oxidative stress conditions. Trial registration number Not applicable

Hematological Studies in Chickens Infected with Hydro Pericardium Syndrome

The present research project was designed to determine the hematological values in infected broiler flocks with hydropericardium syndrome from January to June, 2012 in order to adjoining areas of Hyderabad Sindh. A total of 72 containing population 96,500 broiler chickens were surveyed to observe for hematological variation during the study period. Only 30 (41.66%) flocks were found affected with Hydro pericardium syndrome. Out of these flocks, the incidence of syndrome was recorded as 5.4% during the month of January, 7.41% in February, 10.8% in March, 13% in April, 15.1% in May and 12.8% during the month of June. The hematological studies were conducted in infected broilers with hydropericardium syndrome and found increase in white blood cells (WBC) from 14.500 ± 2.298 to 25.820 ± 844.00 and concentration of erythrocytic sedimentation rate (ESR) from 2.340 ± 0.89 to 3.458 ± 0.291. Amongst Leukocytic count, shThe present research project was designed to determine the hematological values in infected broiler flocks with hydropericardium syndrome from January to June, 2012 in order to adjoining areas of Hyderabad Sindh. A total of 72 containing population 96,500 broiler chickens were surveyed to observe for hematological variation during the study period. Only 30 (41.66%) flocks were found affected with Hydro pericardium syndrome. Out of these flocks, the incidence of syndrome was recorded as 5.4% during the month of January, 7.41% in February, 10.8% in March, 13% in April, 15.1% in May and 12.8% during the month of June. The hematological studies were conducted in infected broilers with hydropericardium syndrome and found increase in white blood cells (WBC) from 14.500 ± 2.298 to 25.820 ± 844.00 and concentration of erythrocytic sedimentation rate (ESR) from 2.340 ± 0.89 to 3.458 ± 0.291. Amongst Leukocytic count, showed marked increase with neutrophils and relative decrease of lymphocytes from 69.360 ± 0.152 to 55.480 ± 2.132, monocytes 4..660 ± 0.598 to 3.340 ± 270, eosinophils 1.496 ± 0.204 to 1.004 ± 0.097 and basophils 0.878 ± 0.55 to 0.734 ± 0.067 in infected birds showed marked increase with neutrophils and relative decrease of lymphocytes from 69.360 ± 0.152 to 55.480 ± 2.132, monocytes 4..660 ± 0.598 to 3.340 ± 270, eosinophils 1.496 ± 0.204 to 1.004 ± 0.097 and basophils 0.878 ± 0.55 to 0.734 ± 0.067 in infected birds.

Molecular Characterization of Chelonid Alphaherpesvirus 5 in a Black Turtle (Chelonia mydas) Fibropapilloma from Baja California Sur, Mexico

During routine monitoring in Ojo de Liebre Lagoon, Mexico, a juvenile black turtle (Chelonia mydas) was captured, physically examined, measured, weighed, sampled, and tagged. The turtle showed no clinical signs suggestive of disease. Eleven months later, this turtle was recaptured in the same area, during which one lesion suggestive of fibropapilloma on the neck was identified and sampled for histopathology and molecular analysis. Histopathology revealed hyperkeratosis, epidermal hyperplasia, acanthosis, papillary differentiation and ballooning degeneration of epidermal cells, increased fibroblasts in the dermis, and angiogenesis, among other things. Hematological values were similar to those reported for clinically healthy black turtles and did not show notable changes between the first capture and the recapture; likewise, clinicopathological evaluation did not show structural or functional damage in the turtle’s systems. The chelonid alphaherpesvirus 5 (ChHV5) UL30 gene was amplified and sequenced for phylogeny; Bayesian reconstruction showed a high alignment with the genus Scutavirus of the Eastern Pacific group. This is one of the first reports of ChHV5 in a cutaneous fibropapilloma of a black turtle in the Baja California peninsula.

The mice’s hematological effect of given the Staphylococcus aureus and Persea americana

Staphylococcus aureus is often the cause of various diseases and food poisoning in animals and humans. Avocado seeds (Persea americana, Mill) contain important compounds that have antimicrobial activity. This study aimed to observe the hematological dynamics of mice (Mus musculus domesticus) infected with S. aureus and induced by P. americana extract. BALB/c male mice aged 12 weeks were divided into 6 groups of 5 in each group, named K-1, K-2, K-3, K-4, K-5, and K-6. All groups were infected with S. aureus 1x108 CFU cell/mL intraperitoneally (IP) on day 0. After the blood sample was taken, then K-1 was given a single dose of tetracyclin 130 mg/kg BW, K-2 was given 130 mg/kb BW oferythromycin, K-3 was given aquabidest (placebo), K-4 was given P. americana extract 300 mg/kg BW, K-5 at a dose 600 mg/kg BW and K-6 at a dose 1200 mg/kg BW. The treatment was given for 7 consecutive days, then a second blood sample was taken and analyzed with the VetScan-HM5 analyzer and interpreted. It was concluded that S. aureus infection caused leukopenia and was potentially followed by lymphopenia, neutrophilia, and monocytosis. The occurrence of microcytosis can lead to anemia, which can be categorized as microcytic hypochromic or normocytic normochromic. Administration of P. americana extract at a dose 300 mg/kg BW (K-4) for 7 days after S. aureus infection was found to be effective in improving hematological values in mice back to normal.