scholarly journals Identification and characterization of an AFLP marker associated with carcass composition in the pig (Brief report)

2006 ◽  
Vol 49 (4) ◽  
pp. 413-414
Author(s):  
E. Muráni ◽  
S. Ponsuksili ◽  
K. Schellander ◽  
K. Wimmers
Keyword(s):  
Fragment Length Polymorphism ◽  
Aflp Marker ◽  
Population Based ◽  
Carcass Composition ◽  
Dna Polymorphisms ◽  
Miniature Pig ◽  
Nucleotide Substitutions ◽  
Single Nucleotide ◽  
Identification And Characterization

Abstract. Amplified Fragment Length Polymorphism (AFLP) is a DNA fingerprinting technique that allows genotyping of DNA polymorphisms, mainly single nucleotide substitutions (SNP), across the whole genome with high multiplex ratio and without the need of their prior knowledge. We have successfully combined AFLP with selective genotyping for mapping of quantitative trait loci (QTL) in a F2 resource population based on Duroc and Berlin Miniature pig (DUMI) (WIMMERS et al., 2002). Here we report application of this approach for the identification of loci associated with carcass composition in a herd of commercial German Landrace pigs.

Assessment of Genetic Diversity of Rheum species (Endangered Medicinal Herb of Indian Himalayan Region) using Molecular Markers

2021 ◽  
Vol 16 (11) ◽  
pp. 147-154
Author(s):  
Anjali Uniyal ◽  
Akhilesh Kumar ◽  
Sweta Upadhyay ◽  
Vijay Kumar ◽  
Sanjay Gupta
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Fragment Length Polymorphism ◽  
Dna Polymorphisms ◽  
Inter Simple Sequence Repeats ◽  
Pharmaceutical Industries ◽  
Endangered Medicinal Herb ◽  
Identification And Characterization ◽  
Simple Sequence

The Rheum species are important medicinal plants that are facing extinction due to their unplanned development and overexploitation by pharmaceutical industries. DNA polymorphisms are not prone to environmental modifications, thus they are widely used for the identification and characterization of plants. The use of different molecular markers has enabled the researchers for the valuation of genetic variability and diversity in its natural zone of distribution. The conventional approach may take several years to yield this information. For the estimation of molecular and genetic variations in geographical zone of distribution, various molecular markers technique are available like RAPD (Randomly Amplified Polymorphic DNA), RFLP (Restriction fragment length polymorphism), ISSR (Inter-Simple Sequence Repeats), SSR and AFLP. The uses of different molecular markers for the study of genetic diversity have been discussed in the review.

Identification and Characterization of the Miniature Pig Huntington's Disease Gene Homolog: Evidence for Conservation and Polymorphism in the CAG Triplet Repeat

Genomics ◽  
2000 ◽  
Vol 69 (1) ◽  
pp. 72-85 ◽  
Author(s):  
Noriko Matsuyama ◽  
Shinji Hadano ◽  
Kyuichiro Onoe ◽  
Hitoshi Osuga ◽  
Junko Showguchi-Miyata ◽  
...  
Keyword(s):  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Disease Gene ◽  
Triplet Repeat ◽  
Miniature Pig ◽  
Identification And Characterization

Identification and characterization of human xylosyltransferase II promoter single nucleotide variants

2015 ◽  
Vol 458 (4) ◽  
pp. 901-907
Author(s):  
Isabel Faust ◽  
Kai Oliver Böker ◽  
Christina Eirich ◽  
Dagmar Akkermann ◽  
Joachim Kuhn ◽  
...  
Keyword(s):  
Single Nucleotide Variants ◽  
Single Nucleotide ◽  
Identification And Characterization

Characterization of genetic polymorphism in the goat β-lactoglobulin gene

2000 ◽  
Vol 67 (2) ◽  
pp. 217-224 ◽  
Author(s):  
RAMONA N. PENA ◽  
ARMAND SÁNCHEZ ◽  
JOSEP M. FOLCH
Keyword(s):  
Fragment Length Polymorphism ◽  
Gene Frequencies ◽  
Single Nucleotide ◽  
Pcr Product ◽  
Polymorphic Position ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Β Lactoglobulin ◽  
New Alleles

Two new variants have been detected and characterized for the goat β-lactoglobulin gene at the cDNA level and confirmed at the genomic level. The two polymorphisms are located on exon 7 of the gene. One of the polymorphic sites is produced by a single nucleotide substitution in position +4601, allowing a polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) genotyping procedure to be developed using SacII restriction enzyme. The other polymorphic position contains a 10 bp long insertion at position +4641 that can be detected by capillary electrophoresis of the PCR product amplified with a fluorescent primer. The association of these two polymorphisms was also investigated, resulting in the description of two new alleles. Both of these contained the point mutation at the SacII site, with or without the 10 bp insertion at position +4641. The distribution of these new polymorphisms was studied in a population of males of four different goat breeds. The gene frequencies for these variants were similar in Spanish and French breeds.

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