scholarly journals Screening the key genes of hair follicle growth cycle in Inner Mongolian Cashmere goat based on RNA sequencing

2020 ◽  
Vol 63 (1) ◽  
pp. 155-164
Author(s):  
Rui Su ◽  
Gao Gong ◽  
Lingtian Zhang ◽  
Xiaochun Yan ◽  
Fenghong Wang ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Rna Sequencing ◽  
Growth Cycle ◽  
Raw Material ◽  
High Price ◽  
Cashmere Goat ◽  
Follicle Growth ◽  
Cashmere Goats ◽  
Hair Follicle Growth ◽  
Upregulated Genes

Abstract. Inner Mongolian Cashmere goat is an excellent local breed selected for the dual-purpose of cashmere and meat. There are three lines of Inner Mongolian Cashmere goat: Erlangshan, Alashan and Aerbasi. Cashmere is a kind of precious textile raw material with a high price. Cashmere is derived from secondary hair follicle (SHF), while hair is derived from primary hair follicle (PHF). The growth cycle of SHF of cashmere goat is 1 year, and it can be divided into three different stages: anagen, catagen and telogen. In this study, we tried to find some important influence factors of SHF growth cycle in skin tissue from Inner Mongolian Cashmere goats by RNA sequencing (RNA-Seq). Three female Aerbasi Inner Mongolian Cashmere goats (2 years old) were used as experimental samples in this study. Skin samples were collected in September (anagen), December (catagen) and March (telogen) at dorsal side from cashmere goats. Results showed that over 511 396 044 raw reads and 487 729 890 clean reads were obtained from sequence data. In total, 51 different expression genes (DEGs) including 29 downregulated genes and 22 upregulated genes were enriched in anagen–catagen comparing group. The 443 DEGs contained 117 downregulated genes and 326 upregulated genes that were enriched in catagen–telogen comparing group. In telogen–anagen comparing group, 779 DEGs were enriched including 582 downregulated genes and 197 upregulated genes. The result of gene ontology (GO) annotation showed that DEGs are in different growth cycle periods, and enriched GO items are mostly related to the transformation of cell and protein. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment result indicated that metabolic process has a great impact on SHF growth cycle. Based on the results of a comprehensive analysis of differentially expressed genes, GO enrichment and KEGG enrichment, we found that FGF5, FGFR1 and RRAS had an effect on the hair follicle growth cycle. The results of this study may provide a theoretical basis for further research on the growth and development of SHF in Inner Mongolian Cashmere goats.

scholarly journals Skin transcriptome reveals the periodic changes in genes underlying cashmere (ground hair) follicle transition in Cashmere goats

2020 ◽  
Author(s):  
Feng Yang ◽  
Zhihong Liu ◽  
Meng Zhao ◽  
Qing Mu ◽  
Tianyu Che ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Growth Period ◽  
Growth Cycle ◽  
Cashmere Goat ◽  
Follicle Growth ◽  
Differential Gene ◽  
Cashmere Goats ◽  
Key Genes ◽  
Hair Follicle Growth ◽  
Resting Period

Abstract Background: Cashmere goats make an outstanding contribution to the livestock textile industry and their cashmere is famous for its slenderness and softness and has been extensively studied. However, there are few reports on the molecular regulatory mechanisms of the secondary hair follicle growth cycle in cashmere goats. In order to explore the regular transition through the follicle cycle and the role of key genes in this cycle, we used a transcriptome sequencing technique to sequence the skin of Inner Mongolian cashmere goats during different months. We analyzed the variation and difference in genes throughout the whole hair follicle cycle. We then verified the regulatory mechanism of the cashmere goat secondary hair follicle growth cycle using fluorescence quantitative PCR. Results: The growth cycle of cashmere hair could be divided into three distinct periods: a growth period (March–September), a regression period (September–December), and a resting period (December–March). The results of differential gene analyses showed that March was the most significant month. Cluster analysis of gene expression throughout the whole growth cycle further supported the key nodes of the three periods of cashmere growth, and the differential gene expression of keratin corresponding to the ground haircashmere growth cycle further supported the results from tissue slices. Quantitative fluorescence analysis showed that KAP3-1, KRTAP 8-1, and KRTAP 24-1 genes had close positive correlation with the cashmere growth cycle, and their regulation was consistent with the growth cycle of cashmere. Conclusion: The growth cycle of cashmere cashmere could be divided into three distinct periods: a growth period (March–September), a regression period (September–December) and a resting period (December–March). March was considered to be the beginning of the cycle. KAP and KRTAP showed close positive correlation with the growth cycle of secondary hair follicle cashmere growth, and their regulation was consistent with the cashmere growth cycle. But hair follicle development-related genes are expressed earlier than cashmere growth, indicating that cycle regulation could alter the temporal growth of cashmere. This study laid a theoretical foundation for the study of the cashmere development cycle and provided evidence for key genes during transition through the cashmere cycle. Our study provides a theoretical basis for cashmere goat breeding.

scholarly journals Skin transcriptome reveals the periodic changes in genes underlying cashmere (ground hair) follicle transition in Cashmere goats

2020 ◽  
Author(s):  
Feng Yang ◽  
Zhihong Liu ◽  
Meng Zhao ◽  
Qing Mu ◽  
Tianyu Che ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Growth Period ◽  
Growth Cycle ◽  
Cashmere Goat ◽  
Follicle Growth ◽  
Differential Gene ◽  
Cashmere Goats ◽  
Key Genes ◽  
Hair Follicle Growth ◽  
Resting Period

Abstract Background: Cashmere goats make an outstanding contribution to the livestock textile industry and their cashmere is famous for its slenderness and softness and has been extensively studied. However, there are few reports on the molecular regulatory mechanisms of the secondary hair follicle growth cycle in cashmere goats. In order to explore the regular transition through the follicle cycle and the role of key genes in this cycle, we used a transcriptome sequencing technique to sequence the skin of Inner Mongolian cashmere goats during different months. We analyzed the variation and difference in genes throughout the whole hair follicle cycle. We then verified the regulatory mechanism of the cashmere goat secondary hair follicle growth cycle using fluorescence quantitative PCR. Results: The growth cycle of cashmere hair could be divided into three distinct periods: a growth period (March–September), a regression period (September–December), and a resting period (December–March). The results of differential gene analyses showed that March was the most significant month. Cluster analysis of gene expression throughout the whole growth cycle further supported the key nodes of the three periods of cashmere growth, and the differential gene expression of keratin corresponding to the ground haircashmere growth cycle further supported the results from tissue slices. Quantitative fluorescence analysis showed that KAP3-1, KRTAP 8-1, and KRTAP 24-1 genes had close positive correlation with the cashmere growth cycle, and their regulation was consistent with the growth cycle of cashmere. Conclusion: The growth cycle of cashmere cashmere could be divided into three distinct periods: a growth period (March–September), a regression period (September–December) and a resting period (December–March). March was considered to be the beginning of the cycle. KAP and KRTAP showed close positive correlation with the growth cycle of secondary hair follicle cashmere growth, and their regulation was consistent with the cashmere growth cycle. But hair follicle development-related genes are expressed earlier than cashmere growth, indicating that cycle regulation could alter the temporal growth of cashmere. This study laid a theoretical foundation for the study of the cashmere development cycle and provided evidence for key genes during transition through the cashmere cycle. Our study provides a theoretical basis for cashmere goat breeding.

Transcriptome sequencing reveals differences between anagen and telogen secondary hair follicle-derived dermal papilla cells of the Cashmere goat (Capra hircus)

2014 ◽  
Vol 46 (3) ◽  
pp. 104-111 ◽  
Author(s):  
Bing Zhu ◽  
Teng Xu ◽  
Zhipeng Zhang ◽  
Na Ta ◽  
Xiaoyu Gao ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Hair Follicle ◽  
Transcriptome Sequencing ◽  
Dermal Papilla ◽  
Growth Cycle ◽  
Hair Follicles ◽  
Capra Hircus ◽  
Cashmere Goat ◽  
Follicle Growth ◽  
Hair Follicle Growth

Dermal papilla is considered the control center of hair follicle growth and hair cycle. The secondary hair follicle (producing cashmere) growth cycle of the Cashmere goat ( Capra hircus) is circannual, and each growth phase can be easily distinguished by its long duration. To identify gene expression patterns and differences of the dermal papilla cell (DPC) between the anagen and telogen phases, we established two DPC lines: ana-DPCs (DPCs derived from the anagen secondary hair follicle) and tel-DPCs (DPCs derived from the telogen secondary hair follicle). Compared with the ana-DPCs, the tel-DPCs lost the capacity to form cell aggregates and showed lower cell proliferation rate. Transcriptome sequencing revealed that 825 genes were differentially expressed by at least threefold between the two DPC lines. These genes were significantly enriched in cell cycle control, cell division, and chromosome partitioning from the Eukaryotic Orthologous Groups of proteins (KOG) database and in cell cycle, cell adhesion molecules, cytokine-cytokine receptor interaction, and p53 signaling pathway from the Kyoto Encyclopedia of Gene and Genomes (KEGG) database. Enrichment analyses revealed that in the middle of the telogen the DPCs of secondary hair follicles (SHFs) seemed on the one hand to promote the degeneration of SHFs and cessation of cashmere growth, while on the other hand to resist self-apoptosis and prepare for the regeneration or revivification of fully functional dermal papillae. These findings provide a better understanding of hair follicle growth and will be useful for identification of novel molecules associated with the control of hair growth cycle.

scholarly journals Expression of Vimentin in hair follicle growth cycle of inner Mongolian Cashmere goats

BMC Genomics ◽  
2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Nai Rile ◽  
Zhihong Liu ◽  
Lixia Gao ◽  
Jingkai Qi ◽  
Meng Zhao ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Growth Cycle ◽  
Follicle Growth ◽  
Cashmere Goats ◽  
Hair Follicle Growth

scholarly journals Skin transcriptome reveals the Periodic changes in genes underlying hair follicle cycling in Cashmere goats

2019 ◽  
Author(s):  
Zhihong Liu ◽  
Feng Yang ◽  
Meng Zhao ◽  
Qing Mu ◽  
Tianyu Che ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hair Follicle ◽  
Growth Cycle ◽  
Molecular Regulation ◽  
Regulation Mechanism ◽  
Follicle Growth ◽  
Differential Gene ◽  
Cashmere Goats ◽  
Hair Follicle Cycle ◽  
Hair Follicle Growth

AbstractCashmere goats, as an important part of animal husbandry production, make outstanding contributions to animal fiber industry. In recent years, a great deal of research has been done on the molecular regulation mechanism of hair follicle cycle growth. However, there are few reports on the molecular regulation mechanisms of secondary hair follicle growth cycle in cashmere goats. In this study, we used transcriptome sequencing technique to sequence the skin of Inner Mongolia cashmere goats in different periods, Analyze the variation and difference of genes in the whole hair follicle cycle. And then, we verified the regulation mechanism of cashmere goat secondary hair follicle growth cycle by fluorescence quantitative PCR. As the result shows: The results of tissue section showed that the growth cycle of cashmere hair could be divided into three distinct periods: growth period (March-September), regression period (September-December) and resting period (December-March). The results of differential gene analysis showed that March was considered the beginning of the cycle, and the difference of gene expression was the most significant. Cluster analysis of gene expression in the whole growth cycle further supported the key nodes of the three periods of villus growth, and the differential gene expression of keratin corresponding to the villus growth cycle further supported the results of tissue slices. Quantitative fluorescence analysis showed that KAP3.1, KRTAP 8-1 and KRTAP 24-1 genes had close positive correlation with the growth cycle of cashmere, and their regulation was consistent with the growth cycle of cashmere. However, there was a sequence of expression time, indicating that the results of cycle regulation made the growth of cashmere change.

Control of the hair follicle growth cycle

2000 ◽  
pp. 83-94
Author(s):  
Ralf Paus ◽  
Sven Müller-Röver ◽  
Ian McKay
Keyword(s):  
Hair Follicle ◽  
Growth Cycle ◽  
Follicle Growth ◽  
Hair Follicle Growth

scholarly journals Melatonin Regulates the Periodic Growth of Cashmere by Upregulating the Expression of Wnt10b and β-catenin in Inner Mongolia Cashmere Goats

2021 ◽  
Vol 12 ◽  
Author(s):  
Junyang Liu ◽  
Qing Mu ◽  
Zhihong Liu ◽  
Yan Wang ◽  
Jiasen Liu ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Inner Mongolia ◽  
Growth Cycle ◽  
Hair Follicles ◽  
Skin Tissue ◽  
Follicle Development ◽  
Periodic Growth ◽  
Cashmere Goats ◽  
Hair Follicle Development ◽  
Hair Follicle Growth

Secondary hair follicle growth in cashmere goats has seasonal cycle changes, and melatonin (MT) has a regulatory effect on the cashmere growth cycle. In this study, the growth length of cashmere was measured by implanting MT in live cashmere goats. The results indicated that the continuous implantation of MT promoted cashmere to enter the anagen 2 months earlier and induce secondary hair follicle development. HE staining of skin tissues showed that the number of secondary hair follicles in the MT-implanted goats was significantly higher than that in the control goats (P < 0.05). Transcriptome sequencing of the skin tissue of cashmere goats was used to identify differentially expressed genes: 532 in February, 641 in October, and 305 in December. Fluorescence quantitative PCR and Western blotting results showed that MT had a significant effect on the expression of Wnt10b, β-catenin, and proteins in the skin tissue of Inner Mongolia cashmere goats. This finding suggested that MT alters the cycle of secondary hair follicle development by changing the expression of related genes. This research lays the foundation for further study on the mechanism by which MT regulates cashmere growth.

scholarly journals Melatonin promotes Cashmere goat (Capra hircus) secondary hair follicle growth: a view from integrated analysis of long non-coding and coding RNAs

Cell Cycle ◽  
2018 ◽  
Vol 17 (10) ◽  
pp. 1255-1267 ◽  
Author(s):  
Wei Ge ◽  
Shan-He Wang ◽  
Bing Sun ◽  
Yue-Lang Zhang ◽  
Wei Shen ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Capra Hircus ◽  
Integrated Analysis ◽  
Cashmere Goat ◽  
Follicle Growth ◽  
Hair Follicle Growth

Promoted Growth of Murine Hair Follicles by Controlled Release of Growth Factors from Biodegradable Hydrogel

2005 ◽  
Vol 288-289 ◽  
pp. 133-138
Author(s):  
Makoto Ozeki ◽  
Yasuhiko Tabata
Keyword(s):  
Growth Factors ◽  
Controlled Release ◽  
Growth Factor ◽  
Hair Follicle ◽  
Skin Color ◽  
Growth Cycle ◽  
Hair Shaft ◽  
Hair Follicles ◽  
Follicle Growth ◽  
Hair Follicle Growth

This study is an investigation to evaluate how the controlled release of different growth factors affects the hair follicle growth of mice in the second anagen stage of hair cycle. For the controlled release of basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF), they were incorporating into biodegradable gelatin hydrogels, while a biodegradable collagen hydrogel was used for incorporation of vascular endothelial growth factor (VEGF). After subcutaneous implantation of the different hydrogels incorporating each growth factor or injection of phosphate buffered saline (PBS) containing the same dose of growth factor into the back of mice, the hair follicle growth was evaluated photometrically and histologically based on four parameters: the skin color of reverse side of the implanted or injected site, the number of vessels newly formed, the area occupied by hair follicle tissue, and the hair length. The area in close proximity to the implanted site of hydrogels incorporating growth factor was still dark in color 10 days after application. The hydrogel incorporating any type of growth factor enabled the hair follicles to increase the size, leading significantly enhanced area occupied by hair follicles per unit area of tissue. Implantation of the hydrogels incorporating growth factor increased significantly the number of blood vessels newly formed. Moreover, the length of hair shaft was elongated by the hydrogel incorporating growth factor to a significantly higher extent than the corresponding growth factor. Neither empty gelatin nor collagen hydrogels affected the hair follicle growth. These results indicate that the hydrogel incorporating growth factor induced the anagen-preservable activity. We conclude that the controlled release enabled growth factors to positively act on the hair growth cycle of mice, irrespective of the factor type.

scholarly journals Skin transcriptome reveals the Periodic changes in genes underlying hair follicle cycling in Cashmere goats

2019 ◽  
Author(s):  
Zhihong Liu ◽  
Feng Yang ◽  
Meng Zhao ◽  
Qing Mu ◽  
Tianyu Che ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hair Follicle ◽  
Growth Period ◽  
Growth Cycle ◽  
Regulation Mechanism ◽  
Wool Follicle ◽  
Differential Gene ◽  
Cashmere Goats ◽  
Hair Follicle Growth ◽  
Cycle Regulation

Abstract Background Cashmere goats, as an important part of animal husbandry production, make outstanding contributions to animal fiber industry. In recent years, a great deal of research has been done on the molecular regulation mechanism of hair follicle cycle growth. However, there are few reports on the molecular regulation mechanisms of secondary hair follicle growth cycle in cashmere goats. In order to explore the changing regularity of wool follicle cycle and the role of key genes in wool follicle cycle of cashmere goats. we used transcriptome sequencing technique to sequence the skin of Inner Mongolia cashmere goats in different periods, Analyze the variation and difference of genes in the whole hair follicle cycle. And then, we verified the regulation mechanism of cashmere goat secondary hair follicle growth cycle by fluorescence quantitative PCR. Results As the result shows: growth period (March-September), regression period (September-December) and resting period (December-March). The results of differential gene analysis showed that March was considered the beginning of the cycle, and the difference of gene expression was the most significant. Cluster analysis of gene expression in the whole growth cycle further supported the key nodes of the three periods of villus growth, and the differential gene expression of keratin corresponding to the villus growth cycle further supported the results of tissue slices. Quantitative fluorescence analysis showed that KAP3-1, KRTAP 8-1 and KRTAP 24-1 genes had close positive correlation with the growth cycle of cashmere, and their regulation was consistent with the growth cycle of cashmere. However, there was a sequence of expression time, indicating that the results of cycle regulation made the growth of cashmere change. Conclusion The growth cycle of cashmere hair could be divided into three distinct periods: growth period (March-September), regression period (September-December) and resting period (December-March).March was considered the beginning of the cycle, KAP and KRTAP had close positive correlation with the growth cycle of cashmere, and their regulation was consistent with the growth cycle of cashmere. However, there was a sequence of expression time, indicating that the results of cycle regulation made the growth of cashmere change.

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