Effect of different soil carbon amendments on the post-harvest dynamics of soil microbial biomass carbon and -nitrogen in an agricultural field experiment
<p>Every year agricultural soils lose significant amounts of nitrogen (N) over winter through N leaching and gas emissions as a result of freeze-thaw cycles. The incorporation of carbon amendments after harvest, such as crop residues or other carbon rich material, can help to promote soil microbial growth, and in doing so, immobilise N within the microbial biomass. It is still unclear which amendments are most effective at promoting microbial N immobilisation and at what time they should be incorporated into the soil to give best results.</p><p>In order to investigate this, we measured soil microbial biomass carbon (C<sub>mic</sub>) and -nitrogen (N<sub>mic</sub>) at 12 timepoints between harvest and spring in soils from an established agricultural field experiment in Kiel (Germany). We selected plots which had the same fertilisation regime and crop rotation (Faba bean-winter wheat-winter barley rotation) but differed in soil carbon amendment treatment; removal of residues (control), wheat straw, faba bean, and sawdust. &#160;In addition to microbial biomass measurements, we measured microbial nutrient limitation at each timepoint via substrate induced respiration, in order to give a qualitative indication of microbial activity in respect to growth limiting nutrients.</p><p>Our data show that there was little effect of wheat straw in comparison to the control on the microbial biomass carbon or -nitrogen, but different patterns were observed for the latter amendments. C<sub>mic</sub> generally decreased over time after harvest in all treatments, but again the decreases were less pronounced in the faba bean and sawdust treatments. N<sub>mic</sub> decreased over time after harvest in control and wheat straw treatment but increased with time in the faba bean and sawdust treatments, suggesting improved N immobilisation by the microbial biomass for these treatments. We found that all soils were nearly always N limited throughout the winter and were never P limited. However, a shift to C limitation was observed after addition of fertiliser in spring, except for in the sawdust treatment, which remained N limited despite the addition of mineral N in the field. This result suggests that sawdust has a higher potential for N immobilisation compared to the other soil amendments.</p><p>In summary, there was little difference in the microbial post-harvest dynamics between the control and wheat straw treatments but stronger effects were observed in the faba bean and saw dust treatments, which suggested improved microbial N immobilisation. Interestingly, the sawdust amendment seemed to have the highest potential for microbial N immobilisation over winter and enduring into spring.</p>