Quantitative Estimation of Long-living Fluorescent Molecules from Temporal Fluorescence Intensity Data Corrupted by Nonzero-mean Noise

Abstract Monoclonal antibody OKT11 was found to compete with sheep red blood cells for binding sites on human lymphocytes. Preincubation of lymphocytes with OKT11 eliminated E-rosette formation. In a study of 142 peripheral blood samples ranging from 1% to over 90% E-rosette- positive cells, comparison to the percent OKT11-positive cells yielded a correlation coefficient of 0.93. In normal donors, subsets of OKT11+ cells were identified using two-color immunofluorescent staining methods with OKT3, OKT4, and OKT8. On the average, approximately 13% of OKT11+ lymphocytes were OKT3- and 13% of OKT11+ lymphocytes were OKT4- and OKT8-. Based on our double antibody fluorescence intensity data, low antigen density OKT11+ lymphocytes were OKT3-. OKT4+ and OKT8+ lymphocytes in normal peripheral lymphocytes have similar OKT11 antigen density.

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All-Dielectric Metasurfaces with High-Fluorescence-Enhancing Capability

Applied Sciences ◽

10.3390/app8081328 ◽

2018 ◽

Vol 8 (8) ◽

pp. 1328 ◽

Cited By ~ 5

Author(s):

Masanobu Iwanaga

Keyword(s):

Fluorescence Intensity ◽

Light Wave ◽

Fluorescence Sensing ◽

Matter Interaction ◽

Light Matter Interaction ◽

High Fluorescence ◽

Rod Array ◽

Fluorescent Molecules ◽

Fluorescence Enhancing ◽

Si Wafer

All-dielectric metasurfaces are an emerging subfield in photonics. Light-wave manipulation has been extensively explored in these metasurfaces. Although light–matter interaction has also been investigated in these metasurfaces, only a limited number of studies have been reported to date. Here, we employ Si-rod-array metasurfaces to examine their fluorescence-enhancing capability. They were designed to have prominent resonances at the working wavelengths of fluorescent molecules. As a result, we experimentally observed significant fluorescence intensity enhancement, exceeding 1000-fold for a reference substrate that was a non-enhancing, flat Si wafer. Thus, we conclude that the all-dielectric metasurfaces can potentially serve as highly fluorescence-enhancing platforms. Their performance is comparable to the best performance reported for metallic metasurfaces. These results strongly suggest that all-dielectric metasurfaces can contribute to fluorescence-sensing of diverse molecules, including biomolecules.

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Monitoring Kinetics of Highly Environment Sensitive States of Fluorescent Molecules by Modulated Excitation and Time-Averaged Fluorescence Intensity Recording

Analytical Chemistry ◽

10.1021/ac0622680 ◽

2007 ◽

Vol 79 (9) ◽

pp. 3330-3341 ◽

Cited By ~ 41

Author(s):

Tor Sandén ◽

Gustav Persson ◽

Per Thyberg ◽

Hans Blom ◽

Jerker Widengren

Keyword(s):

Fluorescence Intensity ◽

Fluorescent Molecules ◽

Kinetics Of

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Measuring Diffusion in Non-Sectioned Articular Cartilage: A FRAP Sensitivity Study

ASME 2008 Summer Bioengineering Conference, Parts A and B ◽

10.1115/sbc2008-192375 ◽

2008 ◽

Author(s):

Jennifer E. Docimo ◽

John E. Novotny

Keyword(s):

Articular Cartilage ◽

Laser Power ◽

Fluorescence Recovery After Photobleaching ◽

Diffusion Constant ◽

Region Of Interest ◽

Small Region ◽

Sensitivity Study ◽

Average Intensity ◽

Intensity Data ◽

Fluorescent Molecules

Measuring the diffusion of molecules within articular cartilage is essential in characterizing its behavior. Information about this important mechanism may be useful to understanding changes in cartilage during degeneration or osteoarthritis. One method used in quantifying diffusion is fluorescence recovery after photobleaching (FRAP). The FRAP technique has been used in previous studies for cartilage [1] and various tissues [2]. In FRAP, a small region of interest (ROI) is selected within the tissue and fluorescent molecules are bleached using a higher laser power than would be used for imaging. Immediately following the ROI bleaching, bleached molecules diffuse out of the ROI as unbleached molecules diffuse into it. The average intensity data within the ROI is collected as a function of time. This data is then fit to a diffusion model usually resulting in a calculation of the diffusion constant, D (μm2/second) [3].

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Method for Analysis and Chemical Confirmation of Sterigmatocystin

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/54.1.86 ◽

1971 ◽

Vol 54 (1) ◽

pp. 86-90 ◽

Cited By ~ 1

Author(s):

Michael Stack ◽

J V Rodricks

Keyword(s):

Silica Gel ◽

Column Chromatography ◽

Fluorescence Intensity ◽

Quantitative Estimation ◽

Spray Reagent ◽

Aqueous Acid ◽

Tlc Plates ◽

Acetate Derivative ◽

Initial Extraction

Abstract A method is described for the analysis of grain samples for the presence and quantitative estimation of sterigmatocystin. Initial extraction with acetonitrile-water (9 + 1) containing KCl is followed by partition of the extract first against hexane and then against CHCl3, and, if needed, silica gel column chromatography. This sequence provides an extract in which the sterigmatocystin can be estimated by fluorescence intensity comparison on TLC plates against a pure sterigmatocystin standard. Visualization of the sterigmatocystin on the TLC plates is enhanced by an AlCl3 spray reagent. After preparative TLC, the identity of sterigmatocystin is confirmed by formation of an acetate derivative and of a derivative formed by treating the suspected extract with aqueous acid.

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Synthesis of new fluorescent molecules having an aggregation-induced emission property derived from 4-fluoroisoxazoles

Beilstein Journal of Organic Chemistry ◽

10.3762/bjoc.16.117 ◽

2020 ◽

Vol 16 ◽

pp. 1411-1417 ◽

Cited By ~ 2

Author(s):

Kazuyuki Sato ◽

Akira Kawasaki ◽

Yukiko Karuo ◽

Atsushi Tarui ◽

Kentaro Kawai ◽

...

Keyword(s):

Fluorescence Intensity ◽

Emission Intensity ◽

Ring Opening ◽

Emission Property ◽

Aggregation Induced Emission ◽

Ring Opening Reaction ◽

Photochemical Properties ◽

Fluorescent Molecules

Fluorescent molecules based on a fluorinated isoxazole scaffold were synthesized and investigated for their photochemical properties. The introduction of a fluorine substituent into 3,5-diarylisoxazoles led to an increase of fluorescence intensity and exhibited a redshift in the emission intensity. α-Fluorinated boron ketoiminates (F-BKIs) were also synthesized via a ring-opening reaction of 4-fluoroisoxazoles and exhibited highly fluorescent luminescence and aggregation-induced emission (AIE), showing promise as a new fluorophore.

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Evaluation of E-rosetting human lymphocytes with OKT11 and other monoclonal antibodies

Blood ◽

10.1182/blood.v60.3.795.bloodjournal603795 ◽

1982 ◽

Vol 60 (3) ◽

pp. 795-799

Author(s):

SH Ip ◽

CW Rittershaus ◽

CC Struzziero ◽

JA Hoxie ◽

RA Hoffman ◽

...

Keyword(s):

Monoclonal Antibody ◽

Monoclonal Antibodies ◽

Fluorescence Intensity ◽

Binding Sites ◽

Blood Cells ◽

Human Lymphocytes ◽

Immunofluorescent Staining ◽

Intensity Data ◽

Blood Samples ◽

Staining Methods

Monoclonal antibody OKT11 was found to compete with sheep red blood cells for binding sites on human lymphocytes. Preincubation of lymphocytes with OKT11 eliminated E-rosette formation. In a study of 142 peripheral blood samples ranging from 1% to over 90% E-rosette- positive cells, comparison to the percent OKT11-positive cells yielded a correlation coefficient of 0.93. In normal donors, subsets of OKT11+ cells were identified using two-color immunofluorescent staining methods with OKT3, OKT4, and OKT8. On the average, approximately 13% of OKT11+ lymphocytes were OKT3- and 13% of OKT11+ lymphocytes were OKT4- and OKT8-. Based on our double antibody fluorescence intensity data, low antigen density OKT11+ lymphocytes were OKT3-. OKT4+ and OKT8+ lymphocytes in normal peripheral lymphocytes have similar OKT11 antigen density.

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