IDENTIFICATION OF SEEDS DIVERSITY IN SUGAR BEET HYBRIDS AND LINES

2021 ◽  
Vol 17 (37) ◽  
pp. 179-188
Author(s):  
Tatiana V. VOSTRIKOVA ◽  
Sergey I. SKACHKOV

Background: Seeds diversity manifests in their heterogeneity according to morphological characteristics and sowing qualities, depending on the genotype and growing conditions. One of the main indicators of the different qualities of beet seeds is the germinative energy and germination capacity, which depend on the genotype. The influence of agrotechnical factors on the diversity of seeds exceeds genetic. Aim: The purpose of the study is to identify the effects of the pre-sowing treatment of the inorganic chemical compound on the diversity of sugar beet (Beta vulgaris L.) seeds for lines and hybrids. Methods: As the signs of the seeds diversity is understood, germinative energy, seedling length, seedling mass. It is presented a method for identifying the genetic diversity of seeds of hybrids and lines of Beta vulgaris, including the treatment of seeds with an aqueous solution of the inorganic chemical substance at a concentration of 10 % with an exposure of 15 s, calculation of criteria for the diversity of seeds: germinative energy, seedling length, seedling mass. Results and Discussion: It was used high concentrations of the inorganic chemical compound for revealing the genetic diversity of seeds. Reactions of genotypes of sugar beet on the provocative factor (the inorganic chemical substance) were different from each other. The inorganic chemical compound is used as a provocative factor and agrotechnical method, and the characteristics of seedling length and seedling mass are taken into account as indicators of the heterogeneity of hybrids and lines in the early stages of plant development. Conclusions: The study allows to reveal the genetic diversity of sugar beet seeds according to the criteria "germination energy" and to assess the heterogeneity of hybrids and lines at early stages of plant development according to the characteristics of seedling length, and seedling mass

2019 ◽  
Author(s):  
Saurav Das ◽  
Tammy Plyler-Harveson ◽  
Dipak K. Santra ◽  
Robert M. Harveson ◽  
Kathy A. Nielsen

AbstractRoot and stem rot caused by Rhizoctonia solani is a serious fungal disease of sugar beet and dry bean production in Nebraska. Objective was to characterize morpho-genetic diversity of 38 Rhizoctonia solani isolated from sugar beet and dry beans fields in western Nebraska over 10 years. Classical morphological features and ISSR marker was used to study the morphogenetic diversity. Fungal colonies were morphologically diverse in shapes, aerial hyphae formation and colony, sclerotia color. Marker analysis using nineteen polymorphic ISSR marker showed polymorphic bands ranged from 15 - 28 with molecular weight 100bp to 3kb. Polymorphic loci ranged from 43.26 – 92.88%. Nei genetic distance within the population was ranged 0.03 –0.09 and Shannon diversity index varied from 0.24 – 0.28. AMOVA analysis based on ΦPT values showed 87% variation within and 13% among the population with statistical significance. Majority of the isolates from sugar beet showed nearby association within the population. There was significant number of cross crop clustering suggesting their broad pathogenicity. Isolates were grouped into three different clusters in UPGMA based cluster analysis using marker information. Interestingly, there was no specific geographical correlation between the isolates. PCA analysis showed randomized distribution among isolates from same geographical origin. Morphological characteristics showed crop-specific two distinct groups of isolates with few exceptions. While, genetic diversity showed two distinct group of isolates, one crop specific and one with wide pathogenicity. This information may help in molecular pathotyping of the pathogen for better disease management.


Agronomie ◽  
1992 ◽  
Vol 12 (7) ◽  
pp. 515-525 ◽  
Author(s):  
J. Boiffin ◽  
C. Durr ◽  
A. Fleury ◽  
A. Marin-Laflèche ◽  
I. Maillet

Author(s):  
Olga Zemlianukhina ◽  
Natalia Cherkasova ◽  
Tatiana Zhuzhzhalova ◽  
Vladislav Kalaev

1995 ◽  
Vol 94 (2) ◽  
pp. 342-350 ◽  
Author(s):  
Steffen Lenzner ◽  
Kurt Zoglauer ◽  
Otto Schieder

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