SALT TOLERANCE INDEX OF TWENTY TWO SUGAR BEET (Beta vulgaris L.) VARIETIES AT EARLY STAGES OF GROWTH

Salinity is a serious abiotic stress to Jute and other crop cultivation at saline regions in the world. No salt tolerant Tossa Jute (Corchorus olitorius L.) variety was developed in Bangladesh. Hence, six Tossa Jute accessions were investigated at germination stage against six concentration levels (0.00 or d.H2O, 8.0, 10.0, 12.0, 14.0 and 16.0 dS m-1) of salt (NaCl) using RCB design at Bangladesh Jute Research Institute (BJRI) during March-July, 2020. Jute seeds collected from Gene Bank of BJRI were allowed to germinate under laboratory condition. Seed germination rate was adversely affected as well as delay in germination was prolonged with increasing the salt concentration. In control, seeds were germinated up to 14.0 dS m-1 salt solution. Among six genotypes, Acc. 1141 and Acc. 3801 showed the highest germination rate (86.67 %); Acc. 3801 gave maximum root length (17.0 mm), dry biomass (6.37 mg); and Acc. 1089 showed higher shoot length (10.0 mm), fresh weight (43.93 mg) and salt tolerance index (60.69 %) under 14.0 dS m-1 level. Higher relative salt harm rate (7.14 %) was observed in both Acc. 1141 and Acc. 3801 under 14.0 dS m-1 salinity indicating highly tolerance to salinity. Acc. 3801 and Acc. 1141 were found good for germination under salt stresses; Acc. 3801, Acc. 1089 for fiber yield and salt tolerance; Acc. 3801 and Acc. 1407 for higher fiber yield. Acc. 3801 was found good for salt tolerance and fiber yield content. The genotypes with good desirable characters would be used as breeding materials to develop high yielding salt tolerant Tossa Jute variety.

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IDENTIFICATION OF SEEDS DIVERSITY IN SUGAR BEET HYBRIDS AND LINES

Periódico Tchê Química ◽

10.52571/ptq.v18.n37.2021.12_vostrikova_pgs_179_188.pdf ◽

2021 ◽

Vol 17 (37) ◽

pp. 179-188

Author(s):

Tatiana V. VOSTRIKOVA ◽

Sergey I. SKACHKOV

Keyword(s):

Genetic Diversity ◽

Sugar Beet ◽

Beta Vulgaris ◽

Plant Development ◽

Chemical Compound ◽

Morphological Characteristics ◽

Chemical Substance ◽

Early Stages ◽

Seedling Length ◽

Inorganic Chemical

Background: Seeds diversity manifests in their heterogeneity according to morphological characteristics and sowing qualities, depending on the genotype and growing conditions. One of the main indicators of the different qualities of beet seeds is the germinative energy and germination capacity, which depend on the genotype. The influence of agrotechnical factors on the diversity of seeds exceeds genetic. Aim: The purpose of the study is to identify the effects of the pre-sowing treatment of the inorganic chemical compound on the diversity of sugar beet (Beta vulgaris L.) seeds for lines and hybrids. Methods: As the signs of the seeds diversity is understood, germinative energy, seedling length, seedling mass. It is presented a method for identifying the genetic diversity of seeds of hybrids and lines of Beta vulgaris, including the treatment of seeds with an aqueous solution of the inorganic chemical substance at a concentration of 10 % with an exposure of 15 s, calculation of criteria for the diversity of seeds: germinative energy, seedling length, seedling mass. Results and Discussion: It was used high concentrations of the inorganic chemical compound for revealing the genetic diversity of seeds. Reactions of genotypes of sugar beet on the provocative factor (the inorganic chemical substance) were different from each other. The inorganic chemical compound is used as a provocative factor and agrotechnical method, and the characteristics of seedling length and seedling mass are taken into account as indicators of the heterogeneity of hybrids and lines in the early stages of plant development. Conclusions: The study allows to reveal the genetic diversity of sugar beet seeds according to the criteria "germination energy" and to assess the heterogeneity of hybrids and lines at early stages of plant development according to the characteristics of seedling length, and seedling mass

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iTRAQ-Based Comparative Proteomic Analysis Provides Insights into Molecular Mechanisms of Salt Tolerance in Sugar Beet (Beta vulgaris L.)

International Journal of Molecular Sciences ◽

10.3390/ijms19123866 ◽

2018 ◽

Vol 19 (12) ◽

pp. 3866 ◽

Cited By ~ 6

Author(s):

Guo-Qiang Wu ◽

Jin-Long Wang ◽

Rui-Jun Feng ◽

Shan-Jia Li ◽

Chun-Mei Wang

Keyword(s):

Salt Tolerance ◽

Sugar Beet ◽

Beta Vulgaris ◽

Molecular Mechanisms ◽

Stress Factors ◽

Pcr Analysis ◽

Absolute Quantitation ◽

Abiotic Stress Factors ◽

Starting Point ◽

Isobaric Tags

Salinity is one of the major abiotic stress factors that limit plant growth and crop yield worldwide. To understand the molecular mechanisms and screen the key proteins in response of sugar beet (Beta vulgaris L.) to salt, in the present study, the proteomics of roots and shoots in three-week-old sugar beet plants exposed to 50 mM NaCl for 72 h was investigated by isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technology. The results showed that 105 and 30 differentially expressed proteins (DEPs) were identified in roots and shoots of salt-treated plants compared with untreated plants, respectively. There were 46 proteins up-regulated and 59 proteins down-regulated in roots; and 13 up-regulated proteins and 17 down-regulated proteins found in shoots, respectively. These DEPs were mainly involved in carbohydrate metabolism, energy metabolism, lipid metabolism, biosynthesis of secondary metabolites, transcription, translation, protein folding, sorting, and degradation as well as transport. It is worth emphasizing that some novel salt-responsive proteins were identified, such as PFK5, MDH, KAT2, ACAD10, CYP51, F3H, TAL, SRPR, ZOG, V-H+-ATPase, V-H+-PPase, PIPs, TIPs, and tubulin α-2/β-1 chain. qRT-PCR analysis showed that six of the selected proteins, including BvPIP1-4, BvVP and BvVAP in root and BvTAL, BvURO-D1, and BvZOG in shoot, displayed good correlation between the expression levels of protein and mRNA. These novel proteins provide a good starting point for further research into their functions using genetic or other approaches. These findings should significantly improve the understanding of the molecular mechanisms involved in salt tolerance of sugar beet plants.

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Seedling Growth of Wheat as Affected by Soil Salinity

Bangladesh Agronomy Journal ◽

10.3329/baj.v20i2.37087 ◽

2018 ◽

Vol 20 (2) ◽

pp. 53-66

Author(s):

E Sultana ◽

MA Hasan ◽

S Sikder ◽

MS Rana ◽

F Alam

Keyword(s):

Salt Tolerance ◽

Seedling Growth ◽

Soil Salinity ◽

Seedling Emergence ◽

Dry Weight ◽

Stress Sensitivity ◽

Saline Stress ◽

Tolerance Index ◽

Wheat Genotypes ◽

Salt Tolerance Index

An experiment was conducted to evaluate seedling growth of wheat under saline condition. Thirty wheat genotypes were grown in trays containing different levels of salinity (Control, 6 and 12 dSm-1) during October to December, 2013. Seedling emergence index, shoot and root length, shoot and root dry weight were found to be reduced with the increases of soil salinity level but the degree of reduction were not similar for all wheat genotypes. Salt tolerance index (STI) also indicated a wide difference in salt tolerance among the wheat genotypes. Sourav, Gourav, Shatabdi, BAW 1185, BAW 1186, BAW 1187, BAW 1189 and BAW 1193 were more salt tolerance while BAW 1177, BAW 1190 and BAW 1198 showed greater salt sensitivity than the other wheat genotypes at 6 dS m-1. However, at 12 dS m-1, Sourav, Gourav, Shatabdi, Sufi and BAW 1184 showed more salt tolerance and BAW 1183, BAW 1190, BAW 1192, BAW 1194 and BAW 97 provided greater stress sensitivity among the testing wheat genotypes. Considering both saline stress Sourav, Gourav and Shatabdi were found to be salt tolerant and BAW 1190 was saline sensitive wheat genotypes.Bangladesh Agron. J. 2017, 20(2): 53-66

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Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa

Scientific Reports ◽

10.1038/s41598-021-84461-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Rakesh Kaundal ◽

Naveen Duhan ◽

Biswa R. Acharya ◽

Manju V. Pudussery ◽

Jorge F. S. Ferreira ◽

...

Keyword(s):

Salt Tolerance ◽

Molecular Mechanisms ◽

Transcriptional Profiling ◽

Shoot Biomass ◽

Tolerance Index ◽

Valuable Insight ◽

Salt Tolerant ◽

Shoot Number ◽

Salt Tolerance Index ◽

High Level

AbstractAlfalfa is an important forage crop that is moderately tolerant to salinity; however, little is known about its salt-tolerance mechanisms. We studied root and leaf transcriptomes of a salt-tolerant (G03) and a salt-sensitive (G09) genotype, irrigated with waters of low and high salinities. RNA sequencing led to 1.73 billion high-quality reads that were assembled into 418,480 unigenes; 35% of which were assigned to 57 Gene Ontology annotations. The unigenes were assigned to pathway databases for understanding high-level functions. The comparison of two genotypes suggested that the low salt tolerance index for transpiration rate and stomatal conductance of G03 compared to G09 may be due to its reduced salt uptake under salinity. The differences in shoot biomass between the salt-tolerant and salt-sensitive lines were explained by their differential expressions of genes regulating shoot number. Differentially expressed genes involved in hormone-, calcium-, and redox-signaling, showed treatment- and genotype-specific differences and led to the identification of various candidate genes involved in salinity stress, which can be investigated further to improve salinity tolerance in alfalfa. Validation of RNA-seq results using qRT-PCR displayed a high level of consistency between the two experiments. This study provides valuable insight into the molecular mechanisms regulating salt tolerance in alfalfa.

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Genome-wide association analysis of cotton salt stress response-related sites

10.1101/2021.09.01.458514 ◽

2021 ◽

Author(s):

Zeliang Zhang ◽

Juyun Zheng ◽

Zhaolong Gong ◽

Yajun Liang ◽

Zhiwei Sang ◽

...

Keyword(s):

Salt Stress ◽

Salt Tolerance ◽

Association Analysis ◽

Upland Cotton ◽

Snp Markers ◽

Tolerance Index ◽

Salt Tolerant ◽

Salt Stress Response ◽

Genome Wide ◽

Salt Tolerance Index

Soil salinization is the main abiotic stress factor affecting agricultural production worldwide, and salt stress has a significant impact on plant growth and development. Cotton is one of the most salt-tolerant crops. Its salt tolerance varies greatly depending on the variety, growth stage, organs, and soil salt types. Therefore, the selection and utilization of excellent salt-tolerant germplasm resources and the excavation of excellent salt-tolerant salt and salt resistance genes play important roles in improving cotton production in saline-alkali soils. In this study, we analysed the population structure and genetic diversity of 144 elite Gossypium hirsutum cultivar accessions collected from around the world, and especially from China. Illumina Cotton SNP 70K was used to obtain genome-wide single-nucleotide polymorphism (SNP) data for 149 experimental materials, and 18,432 highly consistent SNP loci were obtained by filtering. PCA (principal component analysis)indicated that 149 upland cotton materials could be divided into 2 subgroups, including subgroup 1 with 78 materials and subgroup 2 with 71 materials. Using the obtained SNP and other marker genotype test results, under salt stress, the salt tolerance traits 3d_Germination_potential, 3d_Bud_length_drop_rate, 7d_Germination_rate, 7d_Bud_length_drop_rate, 7d_Germination_weight, 3d_Bud_length, 7d_Bud_length, relative_germination_potential, Relative_germination_rate, 7d_Bud_weight_drop_rate, Salt tolerance index 3d_Germination_potential_index, 3d_Bud_length_index, 7d_Bud_length_index, 7d_Bud_weight_index, and 7d_Germination_rate_index were evaluated by genome association analysis. A total of 27 SNP markers closely related to salt tolerance traits and 15 SNP markers closely related to salt tolerance index were detected. At the SNP locus associated with the traits of the bud length decline rate at 7 days, alleles Gh_A01G0034 and Gh_D01G0028 related to plant salt tolerance were detected, and they are related to intracellular transport, membrane microtubule formation and actin network. This study provides a theoretical basis for the selection and breeding of salt-tolerant upland cotton varieties.

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Effects of Nitrogen on Seedling Growth of Wheat Varieties under Salt Stress

Journal of Agricultural Science ◽

10.5539/jas.v8n10p131 ◽

2016 ◽

Vol 8 (10) ◽

pp. 131 ◽

Cited By ~ 1

Author(s):

Muhi Eldeen Hussien Ibrahim ◽

Xinkai Zhu ◽

G. Zhou ◽

Eltayib H. M. A. Abidallhaa

Keyword(s):

Salt Tolerance ◽

Root Length ◽

Dry Weight ◽

Saline Soils ◽

Tolerance Index ◽

Seedling Vigor ◽

Wheat Varieties ◽

Vigor Index ◽

Emergence Percentage ◽

Salt Tolerance Index

<p>Wheat growth is hampered by various environmental stresses including salinity. The aim of the present study was to evaluate the nitrogen effect on seedling emergence and growth under salinity conditions. For this reason the seeds of wheat (<em>Triticum aestivum </em>L.) varieties Argine, and Elnilein from Sudan and Xumai 30, and Yang 10-13 from China were cultured under four NaCl solutions containing (0, 100, 150, and 200 mM NaCl) and three nitrogen (N) fertilizer levels (N0 = 0, N1 = 105, and N2 12 = 210 kg N/h). Emergence percentage and early growth were determined. There were significant differences among salinity and N levels for emergence percentage shoot and root length, dry weight, salt tolerance index, and seedling vigor index. At all salinity levels, the varieties showed similar salt resistance, but each responded differently. Nitrogen affected positively on the seedling characteristics under saline soil. Elnilein had a better emergence percentage, shoot and root length, dry weight, salt tolerance index, and seedling vigor index than the other varieties. Elnilein is recommended for saline soils. We suggest that a simple seedling test would be a useful selection tool in order to develop productive new wheat lines on saline soils.</p>

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Salt Resistance of Tomato (Lycopersicon esculentum Mill.) Cultivars Produced in Benin at Germination Stage

International Journal of Plant & Soil Science ◽

10.9734/ijpss/2019/v28i230102 ◽

2019 ◽

pp. 1-12

Author(s):

Eliane Kinsou ◽

David Montcho ◽

Séraphin Ahissou Zanklan ◽

Julien Koffi Kpinkoun ◽

Françoise Assogba Komlan ◽

...

Keyword(s):

Salt Tolerance ◽

Seed Germination ◽

Germination Rate ◽

Nacl Stress ◽

Salt Resistance ◽

Tolerance Index ◽

Resistance Level ◽

Significant Difference ◽

Salt Tolerance Index ◽

Germination Stage

Aims: In this research study, salt resistance level of seven tomato cultivars grown in Benin, namely Akikon, Tounvi; F1 Mongal, Petomech, Padma, TLCV 15 and Thorgal was evaluated at the germination stage. Study Design: The experiment was laid out as a completely randomized design with four replications. Place and Duration of Study: The experiment was carried out in the Laboratory of Plant Physiology and Abiotic Stresses Study of University of Abomey-Calavi, Republic of Benin from May to June, 2017. Methodology: Seeds were submitted to treatment with four NaCl concentrations (0; 30; 60 and 90 mM NaCl) in Petri dishes. Seed germination was checked every day during ten days incubation period. Four replicates of 40 seeds each were used. Results: NaCl reduced seed germination rate in all cultivars from day 2 to day 10 and the germination index proportionately to NaCl concentration. At the end of the 10 days, salt stress reduced the final germination percentages with a significant difference among cultivars: cultivars F1 Mongal followed by Akikon, Thorgal, TLCV15 and Tounvi were less affected in comparison with the two other cultivars. Salt Tolerance Index was significantly variable according to the cultivar with the highest values for cultivars F1 Mongal (1.086), Akikon (1.028), TLCV15 (1.005) and Tounvi (0.989) and the weakest value for cultivar Petomech (0.436). Conclusion: NaCl stress delayed seed germination and reduced the rate of final germination. Salt Tolerance Index was variable among the seven cultivars. Based on this criterion, cultivars F1 Mongal, Akikon, TLCV15 and Tounvi were the most salt-resistant whereas Petomech was the most salt-sensitive at germination stage.

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Isolation and characterization of halophilic soil fungi from the salt desert of Little Rann of Kutch, India

10.7287/peerj.preprints.1914v1 ◽

2016 ◽

Author(s):

Kartikeya Rajpal ◽

Ram Prasad ◽

Vijai Kumar Gupta ◽

Ajit Varma

Keyword(s):

Salt Tolerance ◽

Morphological Characteristics ◽

Bioactive Molecules ◽

Tolerance Index ◽

Malt Extract ◽

Moderate Halophile ◽

Isolation And Characterization ◽

Rdna Sequencing ◽

The Rich ◽

Salt Tolerance Index

Extremophiles are considered a cornucopian source of chemically diverse and often novel metabolites. Determination of their growth and distribution pattern and their chemical nature is therefore vital to fully gauge their existential uniqueness and potential as a fount of novel compounds. Present study deals with growth characterization and chemoprofiling of halophilic fungi inhabiting a hitherto unexplored terrain of Little Rann of Kutch, India. Thirty five morphologically discrete fungi, primarily belonging to phylum Ascomycota, were isolated from the soil, suggestive of the region’s low microbial diversity. Calculation of fungal salt tolerance index identified one isolate as a halophile with the remaining being halotolerant. Morphological characteristics and 18S rDNA sequencing established the halophile’s identity as Aspergillus versicolor. A. versicolor was further characterized for its salt tolerance potential and degree of halophily. The fungus grew optimally at 7.5% NaCl, thereby classifying it as a moderate halophile. Macro-morphology of the fungus corresponded well with its micro-morphology. At 0% and 10% NaCl, fungal specimen exhibited mycelial distortions, loss of cytoplasm and sparse conidiation; healthy growth features were observed at 7.5% NaCl. For chemoprofiling, A. versicolor was cultured on Czapek Dox broth and Malt Extract broth and the crude extract was analysed by liquid chromatography mass spectrometry (LC-MS). A comparative chemoprofile analysis determined Czapek Dox broth to be a better choice for profiling of this particular strain as it exhibited higher compound diversity. The rich chemical contour of A. versicolor suggests the fungus to be a potential source of novel bioactive molecules of pharmaceutical significance.

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